| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,461,663 | A→G | 50.0% | G301G (GGA→GGG) | gidA → | glucose‑inhibited cell‑division protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,461,663 | 0 | A | G | 50.0% | ‑1.0 / 96.7 | 106 | G301G (GGA→GGG) | gidA | glucose‑inhibited cell‑division protein |
| Reads supporting (aligned to +/- strand): ref base A (49/4); new base G (10/43); total (59/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.64e-15 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 116 total aligned reads displayed.
GATCGAAGACAAAGTCATGCGCTTCGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAG > minE/2461597‑2461723 | gatcgaAGACAAAGTCATGCGCTTCGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGACt > 1:2947022/1‑69 (MQ=255)gatcgaAGACAAAGTCATGCGCTTCGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGACt > 1:2920423/1‑69 (MQ=255)gatcgaAGACAAAGTCATGCGCTTCGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGACt > 1:677003/1‑69 (MQ=255)gatcgaAGACAAAGTCATGCGCTTCGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGACt > 1:2146714/1‑69 (MQ=255)gatcgaAGACAAAGTCATGCGCTTCGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGACt > 1:1244060/1‑69 (MQ=255) cgaAGACAAAGTCATGCGCTTCGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGa < 1:508416/64‑1 (MQ=255) cgaAGACAAAGTCATGCGCTTCGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGa < 1:2860108/64‑1 (MQ=255) cgaAGACAAAGTCATGCGCTTCGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGa < 1:197373/64‑1 (MQ=255) cgaAGACAAAGTCATGCGCTTCGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGa < 1:3172848/64‑1 (MQ=255) cgaAGACAAAGTCATGCGCTTCGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGa < 1:1899397/64‑1 (MQ=255) cgaAGACAAAGTCATGCGCTTCGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGa < 1:1471225/64‑1 (MQ=255) cgaAGACAAAGTCATGCGCTTCGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGa < 1:951855/64‑1 (MQ=255) cgaAGACAAAGTCATGCGCTTCGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGa < 1:1035625/64‑1 (MQ=255) cgaAGACAAAGTCATGCGCTTCGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGACTGAc > 1:2278736/1‑69 (MQ=255) cgaAGACAAAGTCATGCGCTTCGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGACTGAc > 1:1305713/1‑69 (MQ=255) cgaAGACAAAGTCATGCGCTTCGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGACTGAc > 1:1845993/1‑69 (MQ=255) cgaAGACAAAGTCATGCGCTTCGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGACTGAc > 1:3044192/1‑69 (MQ=255) cgaAGACAAAGTCATGCGCTTCGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGACTGAc > 1:2317583/1‑69 (MQ=255) gaAGACAAAGTCATGCGCTTCGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGACTGAc < 1:3226642/68‑1 (MQ=255) cgcTTCGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTAt > 1:2796926/1‑69 (MQ=255) cTTCGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATc > 1:2449035/1‑68 (MQ=255) cTTCGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATc > 1:777639/1‑68 (MQ=255) cTTCGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATc > 1:2704592/1‑68 (MQ=255) cTTCGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATc > 1:1128729/1‑68 (MQ=255) cTTCGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATc > 1:838567/1‑68 (MQ=255) cTTCGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATc > 1:870113/1‑68 (MQ=255) cTTCGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATc > 1:849215/1‑68 (MQ=255) tCGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATCCGa > 1:3409260/1‑69 (MQ=255) tCGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATCCGa > 1:1961185/1‑69 (MQ=255) cGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGAAATTTATCCGaa > 1:661500/1‑69 (MQ=255) cGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGAAATTTATCCGaa > 1:1059796/1‑69 (MQ=255) cGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGAAATTTATCCGaa > 1:3119434/1‑69 (MQ=255) cGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGAAATTTATCCGaa > 1:1003799/1‑69 (MQ=255) cGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGAAATTTATCCGaa > 1:857918/1‑69 (MQ=255) cGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGAAATTTATCCGaa > 1:1062831/1‑69 (MQ=255) cGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGAAATTTATCCGaa > 1:1935978/1‑69 (MQ=255) cGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGAAATTTATCCGaa > 1:513842/1‑69 (MQ=255) cGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGAAATTTATCCGaa > 1:2885619/1‑69 (MQ=255) cGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGGCTGACATCTAACGAAATTTATCCGaa > 1:877584/1‑69 (MQ=255) cGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGAAATTTATCCGAACg < 1:2847136/68‑1 (MQ=255) cGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGAAATTTATCCGAACg < 1:1298133/68‑1 (MQ=255) cGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGAAATTTATCCGAACg < 1:311100/68‑1 (MQ=255) cGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGAAATTTATCCGAACg < 1:1488040/68‑1 (MQ=255) cGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGAAATTTATCCGAACg < 1:771337/68‑1 (MQ=255) cGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGAAATTTATCCGAACg < 1:1064483/68‑1 (MQ=255) cGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGAAATTTATCCGAACg < 1:554866/68‑1 (MQ=255) cGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGAAATTTATCCGAACg < 1:1565415/68‑1 (MQ=255) cGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGAAATTTATCCGAACg < 1:3138459/68‑1 (MQ=255) gACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGAAATTTATCCGAACg < 1:407897/67‑1 (MQ=255) gACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATCCGAACGGt < 1:3093624/69‑1 (MQ=255) aCAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATCCGAACGGTa < 1:43411/69‑1 (MQ=255) aGATCTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATCCGAACGGTAtctc > 1:3419445/1‑58 (MQ=255) aGATCTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATCCGAACGGTAtctc > 1:2790551/1‑58 (MQ=255) aGATCTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATCCGAACGGTAtctc > 1:261406/1‑58 (MQ=255) aGATCTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATCCGAACGGTAtctc > 1:2369010/1‑58 (MQ=255) aGATCTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATCCGAACGGTAtctc > 1:3189238/1‑58 (MQ=255) aGATCTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATCCGAACGGTAtctc > 1:3209019/1‑58 (MQ=255) aGATCTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATCCGAACGGTAtctc > 1:3328173/1‑58 (MQ=255) aGATCTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATCCGAACGGTAtc > 1:3293823/1‑56 (MQ=255) gATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGaaa < 1:421047/38‑1 (MQ=38) gATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGaaa < 1:3037914/38‑1 (MQ=38) gATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGaaa < 1:960816/38‑1 (MQ=38) gATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGaaa < 1:2887380/38‑1 (MQ=38) gATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGaaa < 1:110876/38‑1 (MQ=38) gATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGaaa < 1:949210/38‑1 (MQ=38) gATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGaaa < 1:128514/38‑1 (MQ=38) gATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGaaa < 1:1340552/38‑1 (MQ=38) gATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGaaa < 1:1374013/38‑1 (MQ=38) gATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGaaa < 1:1420104/38‑1 (MQ=38) gATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGaaa < 1:1420283/38‑1 (MQ=38) gATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGaaa < 1:1706440/38‑1 (MQ=38) gATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGaaa < 1:1884817/38‑1 (MQ=38) gATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGaaa < 1:1902937/38‑1 (MQ=38) gATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGaaa < 1:2219123/38‑1 (MQ=38) gATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGaaa < 1:2308313/38‑1 (MQ=38) gATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGaaa < 1:2508339/38‑1 (MQ=38) gATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGaaa < 1:2866280/38‑1 (MQ=38) gATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGaaa < 1:2887348/38‑1 (MQ=38) cTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATCCGATCGGTATCTCCACCAGCCTGCCGt > 1:1643278/1‑68 (MQ=255) cTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATCCGAACGGTAtc > 1:2943073/1‑52 (MQ=255) cTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATCCGAACGGTATCTCCACCCGCCTGCCGt > 1:1218204/1‑68 (MQ=255) cTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATCCGAACGGTATCTCCACCAGCCt > 1:1489215/1‑63 (MQ=255) cTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGt > 1:2753029/1‑68 (MQ=255) cTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGt > 1:482732/1‑68 (MQ=255) cTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGt > 1:470518/1‑68 (MQ=255) cTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGt > 1:466382/1‑68 (MQ=255) cTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGt > 1:2831568/1‑68 (MQ=255) cTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGt > 1:2617141/1‑68 (MQ=255) cTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGt > 1:2294326/1‑68 (MQ=255) cTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGt > 1:2152802/1‑68 (MQ=255) cTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGt > 1:1911685/1‑68 (MQ=255) cTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGt > 1:146878/1‑68 (MQ=255) cTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGt > 1:113703/1‑68 (MQ=255) cTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGt > 1:1004410/1‑68 (MQ=255) ccGGAAGGGCTGACCTCTAACGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAg < 1:3383815/69‑1 (MQ=255) ccGGAAGGGCTGACCTCTAACGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAg < 1:2420923/69‑1 (MQ=255) ccGGAAGGGCTGACCTCTAACGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAg < 1:1045297/69‑1 (MQ=255) ccGGAAGGACTGACCTCTAATGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCa < 1:2319861/68‑1 (MQ=255) cGGAAGGGCTGACCTCTAACGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAg < 1:3274176/68‑1 (MQ=255) cGGAAGGGCTGACCTCTAACGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAg < 1:1911886/68‑1 (MQ=255) | GATCGAAGACAAAGTCATGCGCTTCGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAG > minE/2461597‑2461723 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |