| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,461,675 | T→C | 57.0% | N305N (AAT→AAC) | gidA → | glucose‑inhibited cell‑division protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,461,675 | 0 | T | C | 57.0% | 47.1 / 104.1 | 137 | N305N (AAT→AAC) | gidA | glucose‑inhibited cell‑division protein |
| Reads supporting (aligned to +/- strand): ref base T (40/19); new base C (10/68); total (50/87) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.64e-11 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.81e-01 | |||||||||||
Only 100 of 138 total aligned reads displayed.
CGCTTCGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTC > minE/2461616‑2461740 | cgcTTCGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTAt > 1:2796926/1‑69 (MQ=255)cgcTTCGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTAt > 1:1920076/1‑69 (MQ=255) cTTCGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATc > 1:1128729/1‑68 (MQ=255) cTTCGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATc > 1:870113/1‑68 (MQ=255) cTTCGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATc > 1:2449035/1‑68 (MQ=255) cTTCGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATc > 1:2704592/1‑68 (MQ=255) cTTCGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATc > 1:849215/1‑68 (MQ=255) cTTCGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATc > 1:777639/1‑68 (MQ=255) tCGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATCCGa > 1:145319/1‑69 (MQ=255) tCGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATCCGa > 1:3409260/1‑69 (MQ=255) cGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGAAATTTATCCGaa > 1:513842/1‑69 (MQ=255) cGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGAAATTTATCCGaa > 1:1935978/1‑69 (MQ=255) cGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGAAATTTATCCGaa > 1:857918/1‑69 (MQ=255) cGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGAAATTTATCCGaa > 1:1062831/1‑69 (MQ=255) cGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGAAATTTATCCGaa > 1:1003799/1‑69 (MQ=255) cGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGGCTGACATCTAACGAAATTTATCCGaa > 1:877584/1‑69 (MQ=255) cGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGAAATTTATCCGAACg < 1:311100/68‑1 (MQ=255) cGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGAAATTTATCCGAACg < 1:3138459/68‑1 (MQ=255) cGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGAAATTTATCCGAACg < 1:314786/68‑1 (MQ=255) cGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGAAATTTATCCGAACg < 1:2870945/68‑1 (MQ=255) cGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGAAATTTATCCGAACg < 1:2847136/68‑1 (MQ=255) cGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGAAATTTATCCGAACg < 1:1296865/68‑1 (MQ=255) cGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGAAATTTATCCGAACg < 1:1064483/68‑1 (MQ=255) cGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGAAATTTATCCGAACg < 1:554866/68‑1 (MQ=255) cGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGAAATTTATCCGAACg < 1:771337/68‑1 (MQ=255) gACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGAAATTTATCCGAACg < 1:407897/67‑1 (MQ=255) gACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATCCGAACGGt < 1:3093624/69‑1 (MQ=255) aCAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATCCGAACGGTa < 1:43411/69‑1 (MQ=255) aGATCTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATCCGAACGGTAtctc > 1:261406/1‑58 (MQ=255) aGATCTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATCCGAACGGTAtctc > 1:2369010/1‑58 (MQ=255) aGATCTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATCCGAACGGTAtctc > 1:3189238/1‑58 (MQ=255) aGATCTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATCCGAACGGTAtctc > 1:3328173/1‑58 (MQ=255) aGATCTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATCCGAACGGTAtctc > 1:3419445/1‑58 (MQ=255) aGATCTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATCCGAACGGTAtctc > 1:2790551/1‑58 (MQ=255) aGATCTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATCCGAACGGTAtc > 1:3293823/1‑56 (MQ=255) gATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGaaa < 1:3037914/38‑1 (MQ=38) gATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGaaa < 1:2508339/38‑1 (MQ=38) gATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGaaa < 1:2219123/38‑1 (MQ=38) gATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGaaa < 1:2727078/38‑1 (MQ=38) gATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGaaa < 1:2887380/38‑1 (MQ=38) gATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGaaa < 1:2887348/38‑1 (MQ=38) gATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGaaa < 1:2866280/38‑1 (MQ=38) gATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGaaa < 1:2308313/38‑1 (MQ=38) gATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGaaa < 1:652263/38‑1 (MQ=38) gATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGaaa < 1:1374013/38‑1 (MQ=38) gATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGaaa < 1:1420104/38‑1 (MQ=38) gATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGaaa < 1:1420283/38‑1 (MQ=38) gATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGaaa < 1:1902937/38‑1 (MQ=38) gATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGaaa < 1:1884817/38‑1 (MQ=38) gATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGaaa < 1:1706440/38‑1 (MQ=38) gATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGaaa < 1:168197/38‑1 (MQ=38) gATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGaaa < 1:960816/38‑1 (MQ=38) cTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATCCGATCGGTATCTCCACCAGCCTGCCGt > 1:1643278/1‑68 (MQ=255) cTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATCCGAACGGTAtc > 1:2943073/1‑52 (MQ=255) cTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATCCGAACGGTATCTCCACCCGCCTGCCGt > 1:1218204/1‑68 (MQ=255) cTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATCCGAACGGTATCTCCACCAGCCt > 1:1489215/1‑63 (MQ=255) cTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGt > 1:466382/1‑68 (MQ=255) cTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGt > 1:470518/1‑68 (MQ=255) cTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGt > 1:1004410/1‑68 (MQ=255) cTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGt > 1:2753029/1‑68 (MQ=255) cTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGt > 1:113703/1‑68 (MQ=255) cTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGt > 1:2398750/1‑68 (MQ=255) cTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGt > 1:2294326/1‑68 (MQ=255) cTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGt > 1:146878/1‑68 (MQ=255) ccGGAAGGGCTGACCTCTAACGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAg < 1:1045297/69‑1 (MQ=255) ccGGAAGGGCTGACCTCTAACGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAg < 1:1067252/69‑1 (MQ=255) ccGGAAGGGCTGACCTCTAACGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAg < 1:3383815/69‑1 (MQ=255) ccGGAAGGACTGACCTCTAATGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCa < 1:2319861/68‑1 (MQ=255) cGGAAGGGCTGACCTCTAACGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAg < 1:1911886/68‑1 (MQ=255) cGGAAGGGCTGACCTCTAACGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAg < 1:3274176/68‑1 (MQ=255) ggcTGACCTCTAACGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTTCAGATGCaaa < 1:1617145/67‑1 (MQ=255) ggcTGACCTCTAACGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATTCaaa < 1:2098888/67‑1 (MQ=255) ggcTGACCTCTAACGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCaaa < 1:1653421/67‑1 (MQ=255) ggcTGACCTCTAACGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCaaa < 1:2756091/67‑1 (MQ=255) ggcTGACCTCTAACGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCaaa < 1:454294/67‑1 (MQ=255) ggcTGACCTCTAACGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCaaa < 1:459561/67‑1 (MQ=255) ggcTGACCTCTAACGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCaaa < 1:1454396/67‑1 (MQ=255) ggcTGACCTCTAACGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCaaa < 1:1327405/67‑1 (MQ=255) ggcTGACCTCTAACGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCaaa < 1:1256793/67‑1 (MQ=255) ggcTGACCTCTAACGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCaaa < 1:8500/67‑1 (MQ=255) ggcTGACCTCTAACGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCaaa < 1:1168427/67‑1 (MQ=255) ggcTGACCTCTAACGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCaaa < 1:3317589/67‑1 (MQ=255) ggcTGACCTCTAACGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCaaa < 1:2124274/67‑1 (MQ=255) ggcTGACCTCTAACGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCaaa < 1:3292455/67‑1 (MQ=255) ggcTGACCTCTAACGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCaaa < 1:2746966/67‑1 (MQ=255) ggcTGACCTCTAACGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCaaa < 1:222870/67‑1 (MQ=255) ggcTGACCTCTAACGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCaaa < 1:1839684/67‑1 (MQ=255) ggcTGACCTCTAACGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCaaa < 1:2544780/67‑1 (MQ=255) cTGACCTCTAATGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAg > 1:2779356/1‑60 (MQ=255) tGACCTCTAACGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAg < 1:1516176/59‑1 (MQ=255) ctctAATGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTc < 1:70731/67‑1 (MQ=255) ctctAATGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTc < 1:3026910/67‑1 (MQ=255) ctctAATGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTc < 1:1338766/67‑1 (MQ=255) ctctAATGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTc < 1:3306857/67‑1 (MQ=255) ctctAATGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCg < 1:1296113/69‑1 (MQ=255) ctctAATGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCg < 1:2979650/69‑1 (MQ=255) tctAATGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCg < 1:2610912/68‑1 (MQ=255) aTGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGctc < 1:1292337/67‑1 (MQ=255) aTGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGctc < 1:2158749/67‑1 (MQ=255) aTGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGctc < 1:982116/67‑1 (MQ=255) | CGCTTCGCCGACAGAAATCAGCATCAGATCTTCCTTGAACCGGAAGGACTGACCTCTAATGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTC > minE/2461616‑2461740 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |