| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,461,741 | T→C | 100% | S327S (TCT→TCC) | gidA → | glucose‑inhibited cell‑division protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,461,741 | 0 | T | C | 78.3% | 89.1 / 16.1 | 64 | S327S (TCT→TCC) | gidA | glucose‑inhibited cell‑division protein |
| Reads supporting (aligned to +/- strand): ref base T (3/11); new base C (50/0); total (53/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.90e-10 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TCCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGA > minE/2461697‑2461806 | tcCACCGGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCCATGCAGGGGATGGAAAAcg > 1:4310/1‑64 (MQ=255)tcCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCCATGCAGGGGATGGAAAAcg > 1:275725/1‑64 (MQ=255)tcCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCCATGCAGGGGATGGAAAAcg > 1:2611104/1‑64 (MQ=255)tcCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCCATGCAGGGGATGGAAAAcg > 1:2586610/1‑64 (MQ=255)tcCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCCATGCAGGGGATGGAAAAcg > 1:2462982/1‑64 (MQ=255)tcCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCCATGCAGGGGATGGAAAAcg > 1:314098/1‑64 (MQ=255)tcCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCCATGCAGGGGATGGAAAAcg > 1:3169241/1‑64 (MQ=255)tcCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCCATGCAGGGGATGGAAAAcg > 1:1859496/1‑64 (MQ=255)tcCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCCATGCAGGGGATGGAAAAcg > 1:399715/1‑64 (MQ=255)tcCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCCATGCAGGGGATGGAAAAcg > 1:547827/1‑64 (MQ=255)tcCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCAGCTCCATGCAGGGGATGGAAAACGCGaa > 1:1513834/1‑68 (MQ=255) ttCGATGTGCATATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGt < 1:3335919/69‑1 (MQ=255) ttCGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGt < 1:1921697/69‑1 (MQ=255) ttCGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGt < 1:3243418/69‑1 (MQ=255) ttCGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGt < 1:1462978/69‑1 (MQ=255) tgCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGt < 1:3306251/62‑1 (MQ=255) tgCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGt < 1:1822708/62‑1 (MQ=255) tgCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGt < 1:2659805/62‑1 (MQ=255) tgCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGt < 1:1149149/62‑1 (MQ=255) tgCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGt < 1:1311780/62‑1 (MQ=255) tgCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGt < 1:3210801/62‑1 (MQ=255) tgCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCa > 1:2962138/1‑69 (MQ=255) tgCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCa > 1:1153640/1‑69 (MQ=255) gCAAATCGTCCGCTCTATGCAGGGGATGTAAAACGCGAAGATCGTGCGTCCGGGt < 1:843553/55‑1 (MQ=255) gCAAATCGTCCGCTCCATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGt > 1:2782671/1‑68 (MQ=255) gCAAATCGTCCGCTCCATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGt > 1:3098757/1‑68 (MQ=255) gCAAATCGTCCGCTCCATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGt > 1:2711509/1‑68 (MQ=255) gCAAATCGTCCGCTCCATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGt > 1:1072484/1‑68 (MQ=255) gCAAATCGTCCGCTCCATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGt > 1:2590895/1‑68 (MQ=255) gCAAATCGTCCGCTCCATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGt > 1:2350577/1‑68 (MQ=255) gCAAATCGTCCGCTCCATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGt > 1:818882/1‑68 (MQ=255) gCAAATCGTCCGCTCCATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGt > 1:1908925/1‑68 (MQ=255) gCAAATCGTCCGCTCCATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGt > 1:1343304/1‑68 (MQ=255) gCAAATCGTCCGCTCCATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGt > 1:1812106/1‑68 (MQ=255) gCAAATCGTCCGCTCCATGAAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGACATTGAGt > 1:926996/1‑68 (MQ=255) cGTCCGCTCCATGGAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGAc > 1:421635/1‑67 (MQ=255) cGTCCGCTCCATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATg > 1:2608362/1‑65 (MQ=255) cGTCCGCTCCATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGAct > 1:794865/1‑68 (MQ=255) cGTCCGCTCCATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGAc > 1:737243/1‑67 (MQ=255) cGTCCGCTCCATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGAc > 1:702522/1‑67 (MQ=255) cGTCCGCTCCATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGAc > 1:353135/1‑67 (MQ=255) cGTCCGCTCCATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGAc > 1:614871/1‑67 (MQ=255) cGTCCGCTCCATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGAc > 1:3221797/1‑67 (MQ=255) cGTCCGCTCCATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGAc > 1:53898/1‑67 (MQ=255) cGTCCGCTCCATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGAc > 1:561742/1‑67 (MQ=255) cGTCCGCTCCATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGAc > 1:375526/1‑67 (MQ=255) cGTCCGCTCCATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGAc > 1:2902990/1‑67 (MQ=255) cGTCCGCTCCATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGAc > 1:1031666/1‑67 (MQ=255) cGTCCGCTCCATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGAc > 1:890141/1‑67 (MQ=255) cGTCCGCTCCATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGAc > 1:1369618/1‑67 (MQ=255) cGTCCGCTCCATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGAc > 1:1484118/1‑67 (MQ=255) cGTCCGCTCCATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGAc > 1:1580020/1‑67 (MQ=255) cGTCCGCTCCATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGAc > 1:1582363/1‑67 (MQ=255) cGTCCGCTCCATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGAc > 1:167301/1‑67 (MQ=255) cGTCCGCTCCATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGAc > 1:1755788/1‑67 (MQ=255) cGTCCGCTCCATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGAc > 1:1791402/1‑67 (MQ=255) cGTCCGCTCCATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGAc > 1:1971344/1‑67 (MQ=255) cGTCCGCTCCATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGAc > 1:2044769/1‑67 (MQ=255) cGTCCGCTCCATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGAc > 1:2077749/1‑67 (MQ=255) cGTCCGCTCCATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGAc > 1:2148001/1‑67 (MQ=255) cGTCCGCTCCATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGAc > 1:2239317/1‑67 (MQ=255) cGTCCGCTCCATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGAc > 1:2724670/1‑67 (MQ=255) cGTCCGCTCCATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGAc > 1:2786325/1‑67 (MQ=255) cGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGACttcttc > 1:477927/1‑69 (MQ=255) ctctTTGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGa < 1:3122300/69‑1 (MQ=255) ctctATGGAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGa < 1:2927695/69‑1 (MQ=255) ctctATGCAGTGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGa < 1:3284576/69‑1 (MQ=255) ctctATGCAGGGGATGGAAAACTCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGa < 1:3392683/69‑1 (MQ=255) ctctATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGa < 1:767604/69‑1 (MQ=255) ctctATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGa < 1:990106/69‑1 (MQ=255) ctctATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGa < 1:3418089/69‑1 (MQ=255) ctctATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGa < 1:3325437/69‑1 (MQ=255) ctctATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGa < 1:3177879/69‑1 (MQ=255) ctctATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGa < 1:300887/69‑1 (MQ=255) ctctATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGa < 1:2932381/69‑1 (MQ=255) ctctATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGa < 1:2021157/69‑1 (MQ=255) ctctATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGa < 1:1971311/69‑1 (MQ=255) ctctATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGa < 1:1305459/69‑1 (MQ=255) ctctATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGa < 1:1190590/69‑1 (MQ=255) ctctATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGa < 1:1042356/69‑1 (MQ=255) ctctATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGa < 1:1035999/69‑1 (MQ=255) tctATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGa < 1:2356508/68‑1 (MQ=255) | TCCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGA > minE/2461697‑2461806 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |