| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,461,810 | T→A | 100% | P350P (CCT→CCA) | gidA → | glucose‑inhibited cell‑division protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,461,810 | 0 | T | A | 81.8% | 36.5 / 3.2 | 22 | P350P (CCT→CCA) | gidA | glucose‑inhibited cell‑division protein |
| Reads supporting (aligned to +/- strand): ref base T (4/0); new base A (18/0); total (22/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.83e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGATCCTCGCGACCTGAAACCGACGCTGGAGA > minE/2461745‑2461835 | cAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGATCCt < 1:3322249/66‑1 (MQ=255)cAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGATCCt < 1:2730577/66‑1 (MQ=255) tCGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGATCCTCGCGACCTGAAACCGACGCTGgag > 1:135061/1‑68 (MQ=255) cGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGATCCACGCGACCTGAAACCGACGCTGgaga > 1:25647/1‑68 (MQ=255) cGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGATCCACGCGACCTGAAACCGACGCTGgaga > 1:94946/1‑68 (MQ=255) cGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGATCCACGCGACCTGAAACCGACGCTGgaga > 1:655105/1‑68 (MQ=255) cGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGATCCACGCGACCTGAAACCGACGCTGgaga > 1:589171/1‑68 (MQ=255) cGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGATCCACGCGACCTGAAACCGACGCTGgaga > 1:550616/1‑68 (MQ=255) cGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGATCCACGCGACCTGAAACCGACGCTGgaga > 1:3292497/1‑68 (MQ=255) cGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGATCCACGCGACCTGAAACCGACGCTGgaga > 1:3060688/1‑68 (MQ=255) cGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGATCCACGCGACCTGAAACCGACGCTGgaga > 1:3051864/1‑68 (MQ=255) cGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGATCCACGCGACCTGAAACCGACGCTGgaga > 1:3003717/1‑68 (MQ=255) cGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGATCCACGCGACCTGAAACCGACGCTGgaga > 1:1135684/1‑68 (MQ=255) cGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGATCCACGCGACCTGAAACCGACGCTGgaga > 1:2380279/1‑68 (MQ=255) cGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGATCCACGCGACCTGAAACCGACGCTGgaga > 1:2303731/1‑68 (MQ=255) cGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGATCCACGCGACCTGAAACCGACGCTGgaga > 1:2007175/1‑68 (MQ=255) cGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGATCCACGCGACCTGAAACCGACGCTGgaga > 1:1941655/1‑68 (MQ=255) cGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGATCCACGCGACCTGAAACCGACGCTGgaga > 1:1817694/1‑68 (MQ=255) cGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGATCCACGCGACCTGAAACCGACGCTGgaga > 1:1465353/1‑68 (MQ=255) cGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGATCCACGCGACCTGAAACCGACGCTGgaga > 1:1413720/1‑68 (MQ=255) cGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGATCCACGCGACCTGAAACCGACGCTGgag > 1:2865312/1‑67 (MQ=255) gTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGATCCTc > 1:181825/1‑43 (MQ=255) gTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGATCCTCGCGACCTGAAACCGACGCTGgaga > 1:204547/1‑67 (MQ=255) gTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGATCCTCGCGACCTGAAACCGACGCTGgaga > 1:128305/1‑67 (MQ=255) | CAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGATCCTCGCGACCTGAAACCGACGCTGGAGA > minE/2461745‑2461835 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |