| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,463,067 | A→G | 31.7% | E118E (GAA→GAG) | gidB → | methyltransferase, glucose‑inhibited cell‑division protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,463,067 | 0 | A | G | 31.7% | 66.9 / 42.9 | 85 | E118E (GAA→GAG) | gidB | methyltransferase, glucose‑inhibited cell‑division protein |
| Reads supporting (aligned to +/- strand): ref base A (21/37); new base G (13/14); total (34/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.46e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-02 | |||||||||||
GCCTTGGTAAACGCGTGCGTTTCCTTCGTCAGGTGCAACATGAGCTTAAACTGGAGAATATTGAACCAGTACAGAGCAGGGTAGAAGAGTTTCCTTCAGAGCCGCCATTTGATGGCGTAATT > minE/2463003‑2463124 | gCCTTGGTAAACGCGTGCGTTTCCTTCGTCAGGTGCAACATGAGCTTAAACTGGAGAATATTGAGCCAg < 1:681144/69‑1 (MQ=255)gCCTTGGTAAACGCGTGCGTTTCCTTCGTCAGGTGCAACATGAGCTTAAACTGGAGAATATTGAGCCAg < 1:2908637/69‑1 (MQ=255)gCCTTGGTAAACGCGTGCGTTTCCTTCGTCAGGTGCAACATGAGCTTAAACTGGAGAATATTGAGCCAg < 1:2452462/69‑1 (MQ=255)gCCTTGGTAAACGCGTGCGTTTCCTTCGTCAGGTGCAACATGAGCTTAAACTGGAGAATATTGAGCCAg < 1:587109/69‑1 (MQ=255)gCCTTGGTAAACGCGTGCGTTTCCTTCGTCAGGTGCAACATGAGCTTAAACTGGAGAATATTGAGCCAg < 1:269245/69‑1 (MQ=255)gCCTTGGTAAACGCGTGCGTTTCCTTCGTCAGGTGCAACATGAGCTTAAACTGGAGAATATTGAGCCAg < 1:1441426/69‑1 (MQ=255)gCCTTGGTAAACGCGTGCGTTTCCTTCGTCAGGTGCAACATGAGCTTAAACTGGAGAATATTGAGCCAg < 1:1488395/69‑1 (MQ=255)gCCTTGGTAAACGCGTGCGTTTCCTTCGTCAGGTGCAACATGAGCTTAAACTGGAGAATATTGAGCCAg < 1:2051605/69‑1 (MQ=255)gCCTTGGTAAACGCGTGCGTTTCCTTCGTCAGGTGCAACATGAGCTTAAACTGGAGAATATTGAGCCAg < 1:2014761/69‑1 (MQ=255)gCCTTGGTAAACGCGTGCGTTTCCTTCGTCAGGTGCAACATGAGCTTAAACTGGAGAATATTGAGCCAg < 1:272296/69‑1 (MQ=255)gCCTTGGTAAACGCGTGCGTTTCCTTCGTCAGGTGCAACATGAGCTTAAACTGGAGAATATTGAGCCAg < 1:3166193/69‑1 (MQ=255)gCCTTGGTAAACGCGTGCGTTTCCTTCGTCAGGTGCAACATGAGCTTAAACTGGAGAATATTGAGCCAg < 1:1778419/69‑1 (MQ=255) ccTTGGTAAACGCGTGCGTTTCCTTCGTCAGGTGCAACATGAGCTTAAACTGGAGAATATTGAGCCAg < 1:2733229/68‑1 (MQ=255) ccTTGGTAAACGCGTGCGTTTCCTTCGTCAGGTGCAACATGAGCTTAAACTGGAGAATATTGAGCCAg < 1:3024290/68‑1 (MQ=255) tAAACGCGTGCGTTTCCTTCGTCAGGTGCAACATGAGCTTAAACTGGAGAATATTGAACCAGTACag < 1:2713675/67‑1 (MQ=255) tAAACGCGTGCGTTTCCTTCGTCAGGTGCAACATGAGCTTAAACTGGAGAATATTGAACCAGTACag < 1:3331788/67‑1 (MQ=255) tAAACGCGTGCGTTTCCTTCGTCAGGTGCAACATGAGCTTAAACTGGAGAATATTGAACCAGTACag < 1:2694228/67‑1 (MQ=255) tgcgtTTCCTTCGTCAGGTGCAACATGAGCTTAAACTTGAGAATATTGAACCAGTACAGAGCAGGGt > 1:1828543/1‑67 (MQ=255) tgcgtTTCCTTCGTCAGGTGCAACATGAGCTTAAACTGGAGAATATTGAACCAGTACAGAGCAGGGt > 1:425871/1‑67 (MQ=255) cgtTTCCTTCGTCAGGTGCAACATGAGCTTAATCTGGAGAATATTGAACCAGTACAGAGCAGGGTagaa < 1:430407/69‑1 (MQ=255) cgtTTCCTTCGTCAGGTGCAACATGAGCTTAAACTGGAGAATATTGAACCAGTACAGAGCAGGGTagaa < 1:2997247/69‑1 (MQ=255) cgtTTCCTTCGTCAGGTGCAACATGAGCTTAAACTGGAGAATATTGAACCAGTACAGAGCAGGGTagaa < 1:2215538/69‑1 (MQ=255) cgtTTCCTTCGTCAGGTGCAACATGAGCTTAAACTGGAGAATATTGAACCAGTACAGAGCAGGGTagaa < 1:3037330/69‑1 (MQ=255) cgtTTCCTTCGTCAGGTGCAACATGAGCTTAAACTGGAGAATATTGAACCAGTACAGAGCAGGGTagaa < 1:1697254/69‑1 (MQ=255) cgtTTCCTTCGTCAGGTGCAACATGAGCTTAAACTGGAGAATATTGAACCAGTACAGAGCAGGGTagaa < 1:654394/69‑1 (MQ=255) cgtTTCCTTCGTCAGGTGCAACATGAGCTTAAACTGGAGAATATTGAACCAGTACAGAGCAGGGTagaa < 1:2690220/69‑1 (MQ=255) tCCTTCGTCAGGTGCAACATGAGCTTAAACTGGAGAATATTGAACCAGTACAGAGCAGGGTAGAAGAGt < 1:2557014/69‑1 (MQ=255) tCCTTCGTCAGGTGCAACATGAGCTTAAACTGGAGAATATTGAACCAGTACAGAGCAGGGTAGAAGAGt > 1:3134417/1‑69 (MQ=255) tCCTTCGTCAGGTGCAACATGAGCTTAAACTGGAGAATATTGAACCAGTACAGAGCAGGGTAGAAGAGt < 1:374755/69‑1 (MQ=255) tCCTTCGTCAGGTGCAACATGAGCTTAAACTGGAGAATATTGAACCAGTACAGAGCAGGGTAGAAGAGt > 1:1725900/1‑69 (MQ=255) tCCTTCGTCAGGTGCAACATGAGCTTAAACTGGAGAATATTGAACCAGTACAGAGCAGGGTAGAAGAGt < 1:562182/69‑1 (MQ=255) cTTCGTCAGGTGCAACATTAGCTTAAACTGGAGAATATTGAACCAGTACAGAGCAGGGTAGAAGAGtt < 1:604101/68‑1 (MQ=255) cTTCGTCAGGTGCAACATGAGCTTAAACTGGAGAATATTGAACCAGTACAGAGCAGGGTAGAAGAGtt < 1:796520/68‑1 (MQ=255) cTTCGTCAGGTGCAACATGAGCTTAAACTGGAGAATATTGAACCAGTACAGAGCAGGGTAGAAGAGtt < 1:109096/68‑1 (MQ=255) cTTCGTCAGGTGCAACATGAGCTTAAACTGGAGAATATTGAACCAGTACAGAGCAGGGTAGAAGAGtt < 1:1903988/68‑1 (MQ=255) tCGTCAGGTGCAACATGAGCTTAAACTGGAGAATATTGAACCAGTACAGAGCAGGGTAGAAGAGttta < 1:1378129/68‑2 (MQ=255) tCGTCAGGTGCAACATGAGCTTAAACTGGAGAATATTGAACCAGTACAGAGCAGGGTAGAAGAGTTTc < 1:828490/68‑1 (MQ=255) tCGTCAGGTGCAACATGAGCTTAAACTGGAGAATATTGAACCAGTACAGAGCAGGGTAGAAGAGTTTc < 1:1191164/68‑1 (MQ=255) tCGTCAGGTGCAACATGAGCTTAAACTGGAGAATATTGAACCAGTACAGAGCAGGGTAGAAGAGTTTc < 1:474738/68‑1 (MQ=255) tCGTCAGGTGCAACATGAGCTTAAACTGGAGAATATTGAACCAGTACAGAGCAGGGTAGAAGAGTTTc < 1:301678/68‑1 (MQ=255) tCGTCAGGTGCAACATGAGCTTAAACTGGAGAATATTGAACCAGTACAGAGCAGGGTAGAAGAGTTTc < 1:2722703/68‑1 (MQ=255) cGTCAGGTGCAACATGAGCTTAAACTGGAGAATATTGAACCAGTACAGAGCAGGGTAGAAGAGTTTc < 1:913854/67‑1 (MQ=255) aaCATGAGCTTTTACTGGAGAATATTGAACCAGTACAGAGCAGGGTAGAAGAGTTTc < 1:3256134/57‑1 (MQ=255) aTGAGCTTAAACTGGAGAATATTGAACCAGTACAGAGCAGGGTAGAAGAGTTTCCTTCAGAGCCTCCAt < 1:1204602/69‑1 (MQ=255) gAGCTTAAACTGGAGAATATTGAACCAGTACAGAGCAGGGTAGAAGAGTTTCCTTCAGAGCCGCCAttt < 1:1952438/69‑1 (MQ=255) aGCTTAAACTGGAGAATATTGAACCAGTACAGAGCAGGGTAGAAGAGTTTCCTTCAGAGCTGCCAttt < 1:1996856/68‑1 (MQ=255) aGCTTAAACTGGAGAATATTGAACCAGTACAGAGCAGGGTAGAAGAGTTTCCTTCAGAGCCGCCAttt < 1:677255/68‑1 (MQ=255) aGCTTAAACTGGAGAATATTGAACCAGTACAGAGCAGGGTAGAAGAGTTTCCTTCAGAGCCGCCAttt < 1:3144474/68‑1 (MQ=255) aGCTTAAACTGGAGAATATTGAACCAGTACAGAGCAGGGTAGAAGAGTTTCCTTCAGAGCCGCCATTTg > 1:2456657/1‑69 (MQ=255) aGCTTAAACTGGAGAATATTGAACCAGTACAGAGCAGGGTAGAAGAGTTTCCTTCAGAGCCGCCATTTg > 1:211249/1‑69 (MQ=255) gagaATATTGAACCAGTACAGAGCAGGGTAGAAGTGTTTCCTTCAGAGCCGCCATTTGATGGCGTAAtt > 1:3150063/1‑69 (MQ=255) gagaATATTGAACCAGTACAGAGCAGGGTAGAAGAGTTTCCTTCAGAGCCGCCATTTGATGGCGTAAtt > 1:338224/1‑69 (MQ=255) gagaATATTGAACCAGTACAGAGCAGGGTAGAAGAGTTTCCTTCAGAGCCGCCATTTGATGGCGTAAtt > 1:2585538/1‑69 (MQ=255) gagaATATTGAACCAGTACAGAGCAGGGTAGAAGAGTTTCCTTCAGAGCCGCCATTTGATGGCGTAAtt > 1:3205182/1‑69 (MQ=255) gagaATATTGAACCAGTACAGAGCAGGGTAGAAGAGTTTCCTTCAGAGCCGCCATTTGATGGCGTAAtt > 1:225189/1‑69 (MQ=255) gagaATATTGAACCAGTACAGAGCAGGGTAGAAGAGTTTCCTTCAGAGCCGCCATTTGATGGCGTAAtt > 1:251739/1‑69 (MQ=255) gagaATATTGAACCAGTACAGAGCAGGGTAGAAGAGTTTCCTTCAGAGCCGCCATTTGATGGCGTAAt > 1:2537760/1‑68 (MQ=255) gagaATATTGAACCAGTACAGAGCAGGGTAGAAGAGTTTCCTTCAGAGCCGCCATTTGATGGCGTAAt > 1:2792058/1‑68 (MQ=255) tatTGAACCAGTACAGAGCAGGGTAGAAGAGTTTCCTTCAGAGCCGCCATTTGATGGCGt < 1:3092872/60‑1 (MQ=255) tatTGAACCAGTACAGAGCAGGGTAGAAGAGTTTCCTTCAGAGCCGCCATTTGATGGCGt < 1:2064552/60‑1 (MQ=255) tatTGAACCAGTACAGAGCAGGGTAGAAGAGTTTCCTTCAGAGCCGCCATTTGATGGCGt < 1:3191663/60‑1 (MQ=255) tatTGAACCAGTACAGAGCAGGGTAGAAGAGTTTCCTTCAGAGCCGCCATTTGATGGCGt < 1:2175334/60‑1 (MQ=255) tatTGAACCAGTACAGAGCAGGGTAGAAGAGTTTCCTTCAGAGCCGCCATTTGATGGCGt < 1:858497/60‑1 (MQ=255) tatTGAACCAGTACAGAGCAGGGTAGAAGAGTTTCCTTCAGAGCCGCCATTTGATGGCGt < 1:263317/60‑1 (MQ=255) tatTGAACCAGTACAGAGCAGGGTAGAAGAGTTTCCTTCAGAGCCGCCATTTGATGGCGt < 1:164842/60‑1 (MQ=255) ttGAGCCAGTACATAGTAGGGTAGAAGAGTTTCCGTCAGAGCCGCCATTTGa > 1:3262625/1‑52 (MQ=255) ttGAGCCAGTACAGAGTAGGGTAGAAGAGTTTCCGTCAGAGCCGCCATTTGa > 1:1136190/1‑52 (MQ=255) ttGAGCCAGTACAGAGTAGGGTAGAAGAGTTTCCGTCAGAGCCGCCATTTGa > 1:2515282/1‑52 (MQ=255) ttGAGCCAGTACAGAGTAGGGTAGAAGAGTTTCCGTCAGAGCCGCCATTTGa > 1:564840/1‑52 (MQ=255) ttGAGCCAGTACAGAGTAGGGTAGAAGAGTTTCCGTCAGAGCCGCCATTTGa > 1:1415401/1‑52 (MQ=255) ttGAGCCAGTACAGAGTAGGGTAGAAGAGTTTCCGTCAGAGCCGCCATTTGa > 1:2448611/1‑52 (MQ=255) ttGAGCCAGTACAGAGTAGGGTAGAAGAGTTTCCGTCAGAGCCGCCATTTGa > 1:434724/1‑52 (MQ=255) ttGAGCCAGTACAGAGTAGGGTAGAAGAGTTTCCGTCAGAGCCGCCATTTGa > 1:1694010/1‑52 (MQ=255) ttGAGCCAGTACAGAGTAGGGTAGAAGAGTTTCCGTCAGAGCCGCCATTTGa > 1:38053/1‑52 (MQ=255) ttGAGCCAGTACAGAGTAGGGTAGAAGAGTTTCCGTCAGAGCCGCCATTTGa > 1:1850322/1‑52 (MQ=255) ttGAGCCAGTACAGAGTAGGGTAGAAGAGTTTCCGTCAGAGCCGCCATTTGa > 1:2243143/1‑52 (MQ=255) ttGAGCCAGTACAGAGTAGGGTAGAAGAGTTTCCGTCAGAGCCGCCATTTGa > 1:3038029/1‑52 (MQ=255) ttGAGCCAGTACAGAGTAGGGTAGAAGAGTTTCCGTCAGAGCCGCCATTTGa > 1:2176773/1‑52 (MQ=255) ttGAACCAGTACAGAGCAGGGTAGAAGAGTTTCCtt > 1:3376277/1‑36 (MQ=255) ttGAACCAGTACAGAGCAGGGTAGAAGAGTTTCCtt > 1:2658492/1‑36 (MQ=255) ttGAACCAGTACAGAGCAGGGTAGAAGAGTTTCCtt > 1:329701/1‑36 (MQ=255) ttGAACCAGTACAGAGCAGGGTAGAAGAGTTTCCtt > 1:2382346/1‑36 (MQ=255) ttGAACCAGTACAGAGCAGGGTAGAAGAGTTTCCtt > 1:1014411/1‑36 (MQ=255) ttGAACCAGTACAGAGCAGGGTAGAAGAGTTTCCtt > 1:2845344/1‑36 (MQ=255) ttGAACCAGTACAGAGCAGGGTAGAAGAGTTTCCtt > 1:2182294/1‑36 (MQ=255) | GCCTTGGTAAACGCGTGCGTTTCCTTCGTCAGGTGCAACATGAGCTTAAACTGGAGAATATTGAACCAGTACAGAGCAGGGTAGAAGAGTTTCCTTCAGAGCCGCCATTTGATGGCGTAATT > minE/2463003‑2463124 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |