| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 208,602 | A→T | 27.1% | A204A (GCA→GCT) | dkgB → | 2,5‑diketo‑D‑gluconate reductase B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 208,602 | 0 | A | T | 27.1% | 95.9 / 37.8 | 70 | A204A (GCA→GCT) | dkgB | 2,5‑diketo‑D‑gluconate reductase B |
| Reads supporting (aligned to +/- strand): ref base A (20/31); new base T (11/8); total (31/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.90e-01 | |||||||||||
GTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGCTATGGGGGAAGGTTACTCAGTAATTCCTTCTTCTACTAAACGTAAAAA > minE/208536‑208670 | gTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGcaca < 1:3212439/69‑1 (MQ=255)gTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGcaca < 1:1082987/69‑1 (MQ=255)gTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGcaca < 1:1441760/69‑1 (MQ=255) aGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTc > 1:2383945/1‑69 (MQ=255) aGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTc > 1:1674433/1‑69 (MQ=255) cTGAAAGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCg > 1:2706009/1‑69 (MQ=255) cTGAAAGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCg > 1:1261858/1‑69 (MQ=255) cTGAAAGATAAGGTTATTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCTCAAGTGATTCTGGCg < 1:1027493/69‑1 (MQ=255) cTGAAAGATAAGGTTATTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCTCAAGTGATTCTGGCg < 1:34977/69‑1 (MQ=255) cTGAAAGATAAGGTTATTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCTCAAGTGATTCTGGCg < 1:2300912/69‑1 (MQ=255) cTGAAAGATAAGGTTATTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCTCAAGTGATTCTGGCg < 1:876586/69‑1 (MQ=255) cTGAAAGATAAGGTTATTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCTCAAGTGATTCTGGCg < 1:1080711/69‑1 (MQ=255) cTGAAAGATAAGGTTATTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCTCAAGTGATTCTGGCg < 1:91375/69‑1 (MQ=255) tGAAAGATAAGGTTATTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCTCAAGTGATTCTGGCg < 1:864947/68‑1 (MQ=255) aGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTgg < 1:297059/67‑1 (MQ=255) aGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTgg < 1:962383/67‑1 (MQ=255) aGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTgg < 1:1570975/67‑1 (MQ=255) aGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTgg < 1:2378600/67‑1 (MQ=255) aGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTgg < 1:2223739/67‑1 (MQ=255) aGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTgg < 1:1850376/67‑1 (MQ=255) aGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTgg < 1:2015316/67‑1 (MQ=255) ttaGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGCTAt < 1:2482348/67‑1 (MQ=255) tGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGCTAt < 1:1987090/69‑1 (MQ=255) tGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGCTAt < 1:2538913/69‑1 (MQ=255) tGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGCTAt < 1:916283/69‑1 (MQ=255) tGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGCTAt < 1:271161/69‑1 (MQ=255) tGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGCTAt < 1:2742195/69‑1 (MQ=255) tGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGCTAt < 1:171100/69‑1 (MQ=255) tGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGCTAt < 1:3299781/69‑1 (MQ=255) tGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGCTAt < 1:167051/69‑1 (MQ=255) aGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGCTATgg > 1:2624774/1‑69 (MQ=255) aGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGCTATgg > 1:430610/1‑69 (MQ=255) aGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGCTATgg > 1:1359603/1‑69 (MQ=255) aGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGCTATgg > 1:1005314/1‑69 (MQ=255) aGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGCTATgg > 1:785242/1‑69 (MQ=255) aGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGCTATgg > 1:2820492/1‑69 (MQ=255) aGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGCTATgg > 1:640211/1‑69 (MQ=255) aGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGCTATgg > 1:223263/1‑69 (MQ=255) aGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGCTATgg > 1:739788/1‑69 (MQ=255) aGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGCTATgg > 1:781231/1‑69 (MQ=255) tATTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCTCAAGTGATTCTGGCGTGGGCTATGGGGGa > 1:3023188/1‑69 (MQ=255) tATTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCTCAAGTGATTCTGGCGTGGGCTATGGGGGa > 1:2642548/1‑69 (MQ=255) tATTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCTCAAGTGATTCTGGCGTGGGCTATGGGGGa > 1:1185317/1‑69 (MQ=255) tATTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCTCAAGTGATTCTGGCGTGGGCTATGGGGGa > 1:479746/1‑69 (MQ=255) tATTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCTCAAGTGATTCTGGCGTGGGCTATGGGGGa > 1:568438/1‑69 (MQ=255) tATTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCTCAAGTGATTCTGGCGTGGGCTATGGGGGa > 1:2090795/1‑69 (MQ=255) tATTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCTCAAGTGATTCTGGCGTGGGCTATGGGGGa > 1:1815465/1‑69 (MQ=255) tATTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCTCAAGTGATTCTGGCGTGGGCTATGGGGGa > 1:1701040/1‑69 (MQ=255) tATTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCTCAAGTGATTCTGGCGTGGGCTATGGGGGa > 1:1449512/1‑69 (MQ=255) tATTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCTCAAGTGATTCTGGCGTGGGCTATGGGGGa > 1:1129860/1‑69 (MQ=255) tATTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCTCAAGTGATTCTGGCGTGGGCTATGGGGGa > 1:1376429/1‑69 (MQ=255) tATTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGCTATGGGGGa > 1:2394173/1‑69 (MQ=255) aTTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGc < 1:3371596/59‑1 (MQ=255) aTTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGc < 1:967182/59‑1 (MQ=255) aTTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGc < 1:2901320/59‑1 (MQ=255) aTTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGc < 1:2756094/59‑1 (MQ=255) aTTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGc < 1:2667459/59‑1 (MQ=255) aTTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGc < 1:2436012/59‑1 (MQ=255) aTTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGc < 1:2288097/59‑1 (MQ=255) aTTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGc < 1:2208407/59‑1 (MQ=255) aTTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGc < 1:1946676/59‑1 (MQ=255) aTTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGc > 1:1036083/1‑59 (MQ=255) aTTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGc > 1:1054642/1‑59 (MQ=255) aTTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGc > 1:1099813/1‑59 (MQ=255) aTTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGc > 1:1348831/1‑59 (MQ=255) aTTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGc > 1:1537295/1‑59 (MQ=255) aTTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGc < 1:25109/59‑1 (MQ=255) aTTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGc < 1:357582/59‑1 (MQ=255) ttgCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGc < 1:3206116/47‑1 (MQ=255) gCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGCTATGGGGGAAGGTTACTCAGt < 1:2617151/68‑1 (MQ=255) aaaCACAATGCGACTCCGGCTCAAGTGATTCTGGCg < 1:1207830/36‑1 (MQ=255) tGCGACTCCGGCACAAGTGATTCTGGCGTGGGCTATGGGGGAAGGTTACTCAGt < 1:3055347/54‑1 (MQ=255) gACTCCGGCACAAGTGATTCTGGCGTGGGCTATGGGGGAAGGTTACTCAGt < 1:781029/51‑1 (MQ=255) acaAGTGATTCTGGCGTGGGCTATGGGGGAAGGTTACTCAGTAATTCCTTCTTCTACTAAACGTaaaaa < 1:824086/69‑1 (MQ=255) acaAGTGATTCTGGCGTGGGCTATGGGGGAAGGTTACTCAGTAATTCCTTCTTCTACTAAACGTaaaaa < 1:1127238/69‑1 (MQ=255) | GTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGCTATGGGGGAAGGTTACTCAGTAATTCCTTCTTCTACTAAACGTAAAAA > minE/208536‑208670 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |