| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,464,543 | G→A | 66.0% | A67A (GCG→GCA) | atpB → | F0 sector of membrane‑bound ATP synthase, subunit a |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,464,543 | 0 | G | A | 66.0% | 60.9 / 44.0 | 81 | A67A (GCG→GCA) | atpB | F0 sector of membrane‑bound ATP synthase, subunit a |
| Reads supporting (aligned to +/- strand): ref base G (12/15); new base A (6/47); total (18/63) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.49e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.00e-01 | |||||||||||
TCCATGTTCTTCTCGGTGGTGCTGGGTCTGTTGTTCCTGGTTTTATTCCGTAGCGTAGCCAAAAAGGCGACCAGCGGTGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTA > minE/2464475‑2464601 | tCCATGTTCTTCTCGGTGGTGCTGGGTCTGTTGTTCCTGGTTTTATTCCGTAGCGTAGCCAAAAAGGCg < 1:749206/69‑1 (MQ=255)tCCATGTTCTTCTCGGTGGTGCTGGGTCTGTTGTTCCTGGTTTTATTCCGTAGCGTAGCCAAAAAGGCg < 1:495661/69‑1 (MQ=255)tCCATGTTCTTCTCGGTGGTGCTGGGTCTGTTGTTCCTGGTTTTATTCCGTAGCGTAGCCAAAAAGGCg < 1:2863718/69‑1 (MQ=255)tCCATGTTCTTCTCGGTGGTGCTGGGTCTGTTGTTCCTGGTTTTATTCCGTAGCGTAGCCAAAAAGGCg < 1:170026/69‑1 (MQ=255) ccATGTTCTTCTCGGTGGTGCTGGGTCTGTTGTTCCTGGTTTTATTCCGTAGCGTAGCCAAAAAGGCg < 1:745138/68‑1 (MQ=255) ccATGTTCTTCTCGGTGGTGCTGGGTCTGTTGTTCCTGGTTTTATTCCGTAGCGTAGCCAAAAAGGCg > 1:10237/1‑68 (MQ=255) cATGTTCTTCTCGGTGGTGCTGGGTCTGTTGTTCCTGGTTTTATTCCGTAGCGTAGCCAAAAAGGCg > 1:1888053/1‑67 (MQ=255) cATGTTCTTCTCGGTGGTGCTGGGTCTGTTGTTCCTGGTTTTATTCCGTAGCGTAGCCAAAAAGGCg > 1:1684890/1‑67 (MQ=255) ttcttcTCGGTGGTGCTGGGTCTGTTGTTCCTGGTTTTATTCCGTAGCGTAGCCAAAAAGGCGACCAGc > 1:1609370/1‑69 (MQ=255) ttcttcTCGGTGGTGCTGGGTCTGTTGTTCCTGGTTTTATTCCGTAGCGTAGCCAAAAAGGCGACCAGc > 1:22680/1‑69 (MQ=255) ttcttcTCGGTGGTGCTGGGTCTGTTGTTCCTGGTTTTATTCCGTAGCGTAGCCAAAAAGGCGACCAGc > 1:2798242/1‑69 (MQ=255) ttcttcTCGGTGGTGCTGGGTCTGTTGTTCCTGGTTTTATTCCGTAGCGTAGCCAAAAAGGCGACCAGc > 1:1931486/1‑69 (MQ=255) ttcttcTCGGTGGTGCTGGGTCTGTTGTTCCTGGTTTTATTCCGTAGCGTAGCCAAAAAGGCGACCAGc > 1:1776264/1‑69 (MQ=255) ttcttcTCGGTGGTGCTGGGTCTGTTGTTCCTGGTTTTATTCCGTAGCGTAGCCAAAAAGGCGACCAGc > 1:3363957/1‑69 (MQ=255) ttcttcTCGGTGGTGCTGGGTCTGTTGTTCCTGGTTTTATTCCGTAGCGTAGCCAAAAAGGCGACCAGc > 1:1581254/1‑69 (MQ=255) ttcttcTCGGTGGTGCTGGGTCTGTTGTTCCTGGTTTTATTCCGTAGCGTAGCCAAAAAGGCGACCAGc > 1:1535536/1‑69 (MQ=255) ttcttcTCGGTGGTGCTGGGTCTGTTGTTCCTGGTTTTATTCCGTAGCGTAGCCAAAAAGGCGACCAGc > 1:559901/1‑69 (MQ=255) ttcttcTCGGTGGTGCTGGGTCTGTTGTTCCTGGTTTTATTCCGTAGCGTAGCCAAAAAGGCGACCAGc > 1:490844/1‑69 (MQ=255) ttattctCGGTGGTGCTGGGTCTGTTGTTCCTGGTTTTATTCCGTAGCGTAGCCAAAAAGGCGACCAGc > 1:466801/4‑69 (MQ=255) ggtggtGCTGGGTCTGTTGTTCCTGGTTTTATTCCGTAGTGTAGCCAAAAAGGCAACCAGCGGCGTGcc > 1:1051871/1‑69 (MQ=255) ggtggtGCTGGGTCTGTTGTTCCTGGTTTTATTCCGTAGTGTAGCCAAAAAGGCAACCAGCGGCGTGcc > 1:1951186/1‑69 (MQ=255) ggtggtGCTGGGTCTGTTGTTCCTGGTTTTATTCCGTAGTGTAGCCAAAAAGGCAACCAGCGGCGTGcc > 1:912934/1‑69 (MQ=255) ggtggtGCTGGGTCTGTTGTTCCTGGTTTTATTCCGTAGTGTAGCCAAAAAGGCAACCAGCGGCGTGcc > 1:1236865/1‑69 (MQ=255) ggtggtGCTGGGTCTGTTGTTCCTGGTTTTATTCCGTAGTGTAGCCAAAAAGGCAACCAGCGGCGTGcc > 1:2913635/1‑69 (MQ=255) ggtggtGCTGGGTCTGTTGTTCCTGGTTTTATTCCGTAGTGTAGCCAAAAAGGCAACCAGCGCCGTGcc > 1:2631172/1‑69 (MQ=255) tCTGTTGTTCCTGGTTTTATTCCGTAGTGTAGCCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGttt < 1:465947/67‑1 (MQ=255) tCTGTTGTTCCTGGTTTTATTCCGTAGTGTAGCCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGttt < 1:3137957/67‑1 (MQ=255) tCTGTTGTTCCTGGTTTTATTCCGTAGTGTAGCCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGttt < 1:689547/67‑1 (MQ=255) tCTGTTGTTCCTGGTTTTATTCCGTAGTGTAGCCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGttt < 1:3308366/67‑1 (MQ=255) tCTGTTGTTCCTGGTTTTATTCCGTAGTGTAGCCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGttt < 1:2821417/67‑1 (MQ=255) tCTGTTGTTCCTGGTTTTATTCCGTAGTGTAGCCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGttt < 1:2586080/67‑1 (MQ=255) tCTGTTGTTCCTGGTTTTATTCCGTAGTGTAGCCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGttt < 1:2380945/67‑1 (MQ=255) tCTGTTGTTCCTGGTTTTATTCCGTAGTGTAGCCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGttt < 1:417408/67‑1 (MQ=255) tCTGTTGTTCCTGGTTTTATTCCGTAGTGTAGCCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGttt < 1:2251824/67‑1 (MQ=255) tCTGTTGTTCCTGGTTTTATTCCGTAGTGTAGCCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGttt < 1:2039268/67‑1 (MQ=255) tCTGTTGTTCCTGGTTTTATTCCGTAGTGTAGCCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGttt < 1:2307074/67‑1 (MQ=255) tCTGTTGTTCCTGGTTTTATTCCGTAGTGTAGCCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGttt < 1:19419/67‑1 (MQ=255) tCTGTTGTTCCTGGTTTTATTCCGTAGCGTAGCCAAAAAGGCGACCAGCGGTGTGCCAGGTAAGttt < 1:1142123/67‑1 (MQ=255) tCTGTTGTTCCTGGTTTTATTCCGTAGCGTAGCCAAAAAGGCGACCAGCGGTGTGCCAGGTAAGttt < 1:71188/67‑1 (MQ=255) tCCTGGTTTTATTCCGTAGCGTAGCCAAAAAGGCGACCAGCGGTGTGCCAGGTAAGTTTCAGACcgcg < 1:1633615/68‑1 (MQ=255) tCCTGGTTTTATTCCGTAGCGTAGCCAAAAAGGCGACCAGCGGTGTGCCAGGTAAGTTTCAGACcgcg < 1:3401413/68‑1 (MQ=255) ttATTCCGTAGCGTAGCCAAAAAGGCGACCAGCGGTGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTg > 1:1884471/1‑69 (MQ=255) agcgtagcCAAAAAGGCGACCAGCGGTGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGc < 1:402823/69‑1 (MQ=255) agcgtagcCAAAAAGGCGACCAGCGGTGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGc < 1:2626123/69‑1 (MQ=255) gtagcCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCttt < 1:589068/69‑1 (MQ=255) gtagcCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCttt < 1:3308337/69‑1 (MQ=255) gtagcCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCttt < 1:3281415/69‑1 (MQ=255) gtagcCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCttt < 1:1591593/69‑1 (MQ=255) gtagcCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCttt < 1:452975/69‑1 (MQ=255) gtagcCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCttt < 1:2898666/69‑1 (MQ=255) gtagcCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCttt < 1:909482/69‑1 (MQ=255) gtagcCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCttt < 1:2742253/69‑1 (MQ=255) gtagcCAAAAAGGCAACCAGCGGCGTGCCAGGCAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCttt < 1:803635/69‑1 (MQ=255) gtagcCAAAAAGGC‑AACAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCttt < 1:3133564/68‑1 (MQ=255) gaagcCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCttt < 1:3192376/67‑1 (MQ=255) gcCAAAAAGGCGACCAGCGGTGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGtt < 1:1850019/69‑1 (MQ=255) gcCAAAAAGGCGACCAGCGGTGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGtt < 1:2693911/69‑1 (MQ=255) gcCAAAAAGGCGACCAGCGGTGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGtt < 1:2135086/69‑1 (MQ=255) gcCAAAAAGGCGACCAGCGGTGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGtt < 1:3273290/69‑1 (MQ=255) gcCAAAAAGGCGACCAGCGGTGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGtt < 1:463072/69‑1 (MQ=255) gcCAAAAAGGCGACCAGCGGTGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGtt < 1:1918205/69‑1 (MQ=255) gcCAAAAAGGCGACCAGCGGTGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGtt < 1:1157046/69‑1 (MQ=255) gcCAAAAAGGCGACCAGCGGTGGGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGtt < 1:3318990/69‑1 (MQ=255) ccAAAAAGGCGACCAGCGGTGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGtt < 1:1087085/68‑1 (MQ=255) cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:1381379/68‑1 (MQ=255) cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:1304150/68‑1 (MQ=255) cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:950881/68‑1 (MQ=255) cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:621347/68‑1 (MQ=255) cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:780850/68‑1 (MQ=255) cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:898740/68‑1 (MQ=255) cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:1075419/68‑1 (MQ=255) cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:274505/68‑1 (MQ=255) cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:3138731/68‑1 (MQ=255) cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:2746573/68‑1 (MQ=255) cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:2865399/68‑1 (MQ=255) cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:2942948/68‑1 (MQ=255) cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:2946167/68‑1 (MQ=255) cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:2974260/68‑1 (MQ=255) cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:1419551/68‑1 (MQ=255) cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:3283693/68‑1 (MQ=255) cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:2738739/68‑1 (MQ=255) cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:3360069/68‑1 (MQ=255) cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:377234/68‑1 (MQ=255) cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:1555423/68‑1 (MQ=255) cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:1525201/68‑1 (MQ=255) aaaaaGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:1650167/67‑1 (MQ=255) aaaaGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:2850663/66‑1 (MQ=255) aaGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:802090/64‑1 (MQ=255) ggCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:1286539/62‑1 (MQ=255) | TCCATGTTCTTCTCGGTGGTGCTGGGTCTGTTGTTCCTGGTTTTATTCCGTAGCGTAGCCAAAAAGGCGACCAGCGGTGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTA > minE/2464475‑2464601 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |