| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,464,552 | T→C | 100% | G70G (GGT→GGC) | atpB → | F0 sector of membrane‑bound ATP synthase, subunit a |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,464,552 | 0 | T | C | 75.2% | 88.9 / 22.6 | 72 | G70G (GGT→GGC) | atpB | F0 sector of membrane‑bound ATP synthase, subunit a |
| Reads supporting (aligned to +/- strand): ref base T (1/17); new base C (6/48); total (7/65) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.72e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGTGGTGCTGGGTCTGTTGTTCCTGGTTTTATTCCGTAGCGTAGCCAAAAAGGCGACCAGCGGTGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTAATGGTAGCGTGAAAGAC > minE/2464489‑2464618 | ggtggtGCTGGGTCTGTTGTTCCTGGTTTTATTCCGTAGTGTAGCCAAAAAGGCAACCAGCGGCGTGcc > 1:1951186/1‑69 (MQ=255)ggtggtGCTGGGTCTGTTGTTCCTGGTTTTATTCCGTAGTGTAGCCAAAAAGGCAACCAGCGGCGTGcc > 1:2913635/1‑69 (MQ=255)ggtggtGCTGGGTCTGTTGTTCCTGGTTTTATTCCGTAGTGTAGCCAAAAAGGCAACCAGCGGCGTGcc > 1:1051871/1‑69 (MQ=255)ggtggtGCTGGGTCTGTTGTTCCTGGTTTTATTCCGTAGTGTAGCCAAAAAGGCAACCAGCGGCGTGcc > 1:1236865/1‑69 (MQ=255)ggtggtGCTGGGTCTGTTGTTCCTGGTTTTATTCCGTAGTGTAGCCAAAAAGGCAACCAGCGGCGTGcc > 1:912934/1‑69 (MQ=255)ggtggtGCTGGGTCTGTTGTTCCTGGTTTTATTCCGTAGTGTAGCCAAAAAGGCAACCAGCGCCGTGcc > 1:2631172/1‑69 (MQ=255) tCTGTTGTTCCTGGTTTTATTCCGTAGTGTAGCCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGttt < 1:465947/67‑1 (MQ=255) tCTGTTGTTCCTGGTTTTATTCCGTAGTGTAGCCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGttt < 1:19419/67‑1 (MQ=255) tCTGTTGTTCCTGGTTTTATTCCGTAGTGTAGCCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGttt < 1:2039268/67‑1 (MQ=255) tCTGTTGTTCCTGGTTTTATTCCGTAGTGTAGCCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGttt < 1:2251824/67‑1 (MQ=255) tCTGTTGTTCCTGGTTTTATTCCGTAGTGTAGCCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGttt < 1:2307074/67‑1 (MQ=255) tCTGTTGTTCCTGGTTTTATTCCGTAGTGTAGCCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGttt < 1:2380945/67‑1 (MQ=255) tCTGTTGTTCCTGGTTTTATTCCGTAGTGTAGCCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGttt < 1:2586080/67‑1 (MQ=255) tCTGTTGTTCCTGGTTTTATTCCGTAGTGTAGCCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGttt < 1:3308366/67‑1 (MQ=255) tCTGTTGTTCCTGGTTTTATTCCGTAGTGTAGCCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGttt < 1:2821417/67‑1 (MQ=255) tCTGTTGTTCCTGGTTTTATTCCGTAGTGTAGCCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGttt < 1:417408/67‑1 (MQ=255) tCTGTTGTTCCTGGTTTTATTCCGTAGTGTAGCCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGttt < 1:3137957/67‑1 (MQ=255) tCTGTTGTTCCTGGTTTTATTCCGTAGTGTAGCCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGttt < 1:689547/67‑1 (MQ=255) tCTGTTGTTCCTGGTTTTATTCCGTAGCGTAGCCAAAAAGGCGACCAGCGGTGTGCCAGGTAAGttt < 1:1142123/67‑1 (MQ=255) tCTGTTGTTCCTGGTTTTATTCCGTAGCGTAGCCAAAAAGGCGACCAGCGGTGTGCCAGGTAAGttt < 1:71188/67‑1 (MQ=255) tCCTGGTTTTATTCCGTAGCGTAGCCAAAAAGGCGACCAGCGGTGTGCCAGGTAAGTTTCAGACcgcg < 1:3401413/68‑1 (MQ=255) tCCTGGTTTTATTCCGTAGCGTAGCCAAAAAGGCGACCAGCGGTGTGCCAGGTAAGTTTCAGACcgcg < 1:1633615/68‑1 (MQ=255) ttATTCCGTAGCGTAGCCAAAAAGGCGACCAGCGGTGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTg > 1:1884471/1‑69 (MQ=255) agcgtagcCAAAAAGGCGACCAGCGGTGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGc < 1:2626123/69‑1 (MQ=255) agcgtagcCAAAAAGGCGACCAGCGGTGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGc < 1:402823/69‑1 (MQ=255) gtagcCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCttt < 1:1591593/69‑1 (MQ=255) gtagcCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCttt < 1:3308337/69‑1 (MQ=255) gtagcCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCttt < 1:589068/69‑1 (MQ=255) gtagcCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCttt < 1:2742253/69‑1 (MQ=255) gtagcCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCttt < 1:3281415/69‑1 (MQ=255) gtagcCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCttt < 1:909482/69‑1 (MQ=255) gtagcCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCttt < 1:452975/69‑1 (MQ=255) gtagcCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCttt < 1:2898666/69‑1 (MQ=255) gtagcCAAAAAGGCAACCAGCGGCGTGCCAGGCAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCttt < 1:803635/69‑1 (MQ=255) gtagcCAAAAAGGC‑AACAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCttt < 1:3133564/68‑1 (MQ=255) gaagcCAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCttt < 1:3192376/67‑1 (MQ=255) gcCAAAAAGGCGACCAGCGGTGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGtt < 1:1918205/69‑1 (MQ=255) gcCAAAAAGGCGACCAGCGGTGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGtt < 1:1157046/69‑1 (MQ=255) gcCAAAAAGGCGACCAGCGGTGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGtt < 1:463072/69‑1 (MQ=255) gcCAAAAAGGCGACCAGCGGTGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGtt < 1:2693911/69‑1 (MQ=255) gcCAAAAAGGCGACCAGCGGTGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGtt < 1:3273290/69‑1 (MQ=255) gcCAAAAAGGCGACCAGCGGTGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGtt < 1:2135086/69‑1 (MQ=255) gcCAAAAAGGCGACCAGCGGTGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGtt < 1:1850019/69‑1 (MQ=255) gcCAAAAAGGCGACCAGCGGTGGGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGtt < 1:3318990/69‑1 (MQ=255) ccAAAAAGGCGACCAGCGGTGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGtt < 1:1087085/68‑1 (MQ=255) cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:377234/68‑1 (MQ=255) cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:621347/68‑1 (MQ=255) cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:780850/68‑1 (MQ=255) cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:898740/68‑1 (MQ=255) cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:3360069/68‑1 (MQ=255) cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:950881/68‑1 (MQ=255) cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:2865399/68‑1 (MQ=255) cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:3138731/68‑1 (MQ=255) cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:1075419/68‑1 (MQ=255) cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:1304150/68‑1 (MQ=255) cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:1381379/68‑1 (MQ=255) cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:1419551/68‑1 (MQ=255) cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:1525201/68‑1 (MQ=255) cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:1555423/68‑1 (MQ=255) cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:2738739/68‑1 (MQ=255) cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:274505/68‑1 (MQ=255) cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:2746573/68‑1 (MQ=255) cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:2942948/68‑1 (MQ=255) cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:2946167/68‑1 (MQ=255) cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:2974260/68‑1 (MQ=255) cAAAAAGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:3283693/68‑1 (MQ=255) aaaaaGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:1650167/67‑1 (MQ=255) aaaaGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:2850663/66‑1 (MQ=255) aaGGCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:802090/64‑1 (MQ=255) ggCAACCAGCGGCGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTa < 1:1286539/62‑1 (MQ=255) ggTGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTAATGGTAGCGTGAAAGAc < 1:1713093/69‑1 (MQ=255) ggTGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTAATGGTAGCGTGAAAGAc < 1:877557/69‑1 (MQ=255) | GGTGGTGCTGGGTCTGTTGTTCCTGGTTTTATTCCGTAGCGTAGCCAAAAAGGCGACCAGCGGTGTGCCAGGTAAGTTTCAGACCGCGATTGAGCTGGTGATCGGCTTTGTTAATGGTAGCGTGAAAGAC > minE/2464489‑2464618 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |