| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,464,777 | G→T | 53.2% | A145A (GCG→GCT) | atpB → | F0 sector of membrane‑bound ATP synthase, subunit a |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,464,777 | 0 | G | T | 53.2% | ‑2.9 / 169.8 | 141 | A145A (GCG→GCT) | atpB | F0 sector of membrane‑bound ATP synthase, subunit a |
| Reads supporting (aligned to +/- strand): ref base G (15/51); new base T (25/50); total (40/101) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 | |||||||||||
Only 100 of 143 total aligned reads displayed.
TCGACCTGCTGCCGTACATTGCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCGGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTATTTATCCTGATTCTGTTCTACAGCATCAA > minE/2464710‑2464842 | tCGACCTGCTGCCGTACATTGCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCgg > 1:3257733/1‑69 (MQ=255)tCGACCTGCTGCCGTACATTGCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCg > 1:1064944/1‑68 (MQ=255) gACCTGCTGCCGTACATTGCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCGGAc > 1:353165/1‑69 (MQ=255) gACCTGCTGCCGTACATTGCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCGGAc > 1:234453/1‑69 (MQ=255) ctgctgCCGTACATTGCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCGGACGTg < 1:779092/69‑1 (MQ=255) ctgctgCCGTACATTGCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCGGACGTg < 1:387471/69‑1 (MQ=255) ctgctgCCGTACATTGCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCGGACGTg < 1:1759456/69‑1 (MQ=255) ctgctgCCGTACATTGCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCGGACGTg < 1:224178/69‑1 (MQ=255) ctgctgCCGTACATTGCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCGGACGTg < 1:1514104/69‑1 (MQ=255) ctgctgCCGTACATTGCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCGGACGTg < 1:2857345/69‑1 (MQ=255) gTACATTGCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTaa < 1:1756251/68‑1 (MQ=255) gTACATTGCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTaa < 1:2365390/68‑1 (MQ=255) gTACATTGCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTaa < 1:2445931/68‑1 (MQ=255) gTACATTGCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTaa < 1:1409122/68‑1 (MQ=255) gTACATTGCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTaa < 1:2917558/68‑1 (MQ=255) gTACATTGCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTaa < 1:293296/68‑1 (MQ=255) gTACATTGCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTaa < 1:1357666/68‑1 (MQ=255) gTACATTGCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTaa < 1:113247/68‑1 (MQ=255) gTACATTGCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTaa < 1:1157903/68‑1 (MQ=255) tACATTGCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTaa < 1:2970134/67‑1 (MQ=255) tACATTGCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTaa < 1:3380714/67‑1 (MQ=255) tACATTGCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTaa < 1:3247106/67‑1 (MQ=255) aCATTGCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCGGACGTGAACGTAACGc < 1:2685051/69‑1 (MQ=255) aCATTGCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCGGACGTGAACGTAACGc < 1:240198/69‑1 (MQ=255) aCATTGCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCGGACGTGAACGTAACGc < 1:3414515/69‑1 (MQ=255) tGCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCGGACGTGAACGTAACGCTGTc < 1:1228290/69‑1 (MQ=255) gCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCGGACGTGAACGTAACGCTGTc < 1:1503133/68‑1 (MQ=255) gCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCGGACGTGAACGTAACGCTGTc < 1:1441355/68‑1 (MQ=255) gAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCGGACGTGAACGTAACGCTGTCTATg < 1:1443686/69‑1 (MQ=255) cATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCGGACGTGAACGTAACGCTGTCTATGGc < 1:2229543/68‑1 (MQ=255) cATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCGGACGTGAACGTAACGCTGTCTATGGc < 1:3242575/68‑1 (MQ=255) cATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCGGACGTGAACGTAACGCTGTCTATGGc < 1:972370/68‑1 (MQ=255) cATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCGGACGTGAACGTAACGCTGTCTATGGc < 1:108346/68‑1 (MQ=255) aTGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCGGACGTGAACGTAACGCTGTc < 1:1379554/61‑1 (MQ=255) gTCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa > 1:1724494/1‑68 (MQ=255) gTCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa > 1:2728735/1‑68 (MQ=255) gTCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa > 1:2090457/1‑68 (MQ=255) gTCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa > 1:1324500/1‑68 (MQ=255) gTCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa > 1:3006451/1‑68 (MQ=255) gTCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa > 1:794675/1‑68 (MQ=255) gTCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa > 1:494429/1‑68 (MQ=255) gTCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa > 1:1121885/1‑68 (MQ=255) gTCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa > 1:490378/1‑68 (MQ=255) tCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa < 1:2798551/67‑1 (MQ=255) tCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa < 1:2673414/67‑1 (MQ=255) tCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa < 1:2849916/67‑1 (MQ=255) tCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa < 1:1398580/67‑1 (MQ=255) tCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa < 1:1286090/67‑1 (MQ=255) tCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa < 1:3362584/67‑1 (MQ=255) tCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa < 1:3281362/67‑1 (MQ=255) tCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa < 1:1369818/67‑1 (MQ=255) tCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa < 1:3051789/67‑1 (MQ=255) tCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa < 1:3251417/67‑1 (MQ=255) tCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa < 1:3191295/67‑1 (MQ=255) tCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa < 1:135690/67‑1 (MQ=255) tCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa < 1:339813/67‑1 (MQ=255) tCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa < 1:2714079/67‑1 (MQ=255) tCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa < 1:1263612/67‑1 (MQ=255) tCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa < 1:2636539/67‑1 (MQ=255) tCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa < 1:2507744/67‑1 (MQ=255) tCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa < 1:1589490/67‑1 (MQ=255) tCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa < 1:811166/67‑1 (MQ=255) tCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa < 1:2338158/67‑1 (MQ=255) tCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa < 1:841341/67‑1 (MQ=255) tCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa < 1:8610/67‑1 (MQ=255) tCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa < 1:877005/67‑1 (MQ=255) tCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa < 1:2082807/67‑1 (MQ=255) tCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa < 1:1893855/67‑1 (MQ=255) tCTGCCTGCACTGCGTGTGGTTCCGTCTGCGGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTAtt > 1:1026867/1‑69 (MQ=255) tCTGCCTGCACTGCGTGTGGTTCCGTCTGCGGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTAtt > 1:3209189/1‑69 (MQ=255) tCTGCCTGCACTGCGTGTGGTTCCGTCTGCGGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTAtt > 1:2147109/1‑69 (MQ=255) tCTGCCTGCACTGCGTGTGATTCCGTCTGCGGACGTGAAc > 1:1489472/1‑40 (MQ=255) tgcctgcACTGCGTGTGGTTCCGTCTGCGGACGTGAACGTAACGc < 1:1634245/45‑1 (MQ=255) tgcACTGTGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTATTTATCCt > 1:1327993/1‑69 (MQ=255) tgcACTGTGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTATTTATCCt > 1:3037378/1‑69 (MQ=255) tgcACTGTGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTATTTATCCt > 1:921364/1‑69 (MQ=255) tgcACTGTGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTATTTATCCt > 1:2885698/1‑69 (MQ=255) tgcACTGTGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTATTTATCCt > 1:696513/1‑69 (MQ=255) tgcACTGTGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTATTTATCCt > 1:629639/1‑69 (MQ=255) tgcACTGTGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTATTTATCCt > 1:453189/1‑69 (MQ=255) tgcACTGTGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGAACTGGGCGTATTTATCCt > 1:1590053/1‑69 (MQ=255) gCGTGTGGTTCCGTCTGCGGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTATTTATCCTGATTCt < 1:1954295/69‑1 (MQ=255) gCGTGTGGTTCCGTCTGCGGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTATTTATCCTGATTCt < 1:711964/69‑1 (MQ=255) gCGTGTGGTTCCGTCTGCGGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTATTTATCCTGATTCt < 1:3125383/69‑1 (MQ=255) gCGTGTGGTTCCGTCTGCGGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTATTTATCCTGATTCt < 1:2767602/69‑1 (MQ=255) gCGTGTGGTTCCGTCTGCGGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTATTTATCCTGATTCt < 1:2546649/69‑1 (MQ=255) gCGTGTGGTTCCGTCTGCGGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTATTTATCCTGATTCt < 1:369863/69‑1 (MQ=255) cGTGTGGTTCCGTCTGCGGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTATTTATCCTGATTCt < 1:742568/68‑1 (MQ=255) gTTCCGTCTGCGGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTATTTATCCTGATTCTg < 1:45987/63‑1 (MQ=255) gTTCCGTCTGCGGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTATTTATCCTGATTCTg < 1:1557490/63‑1 (MQ=255) gTTCCGTCTGCGGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTATTTATCCTGATTCTg < 1:2381528/63‑1 (MQ=255) gTTCCGTCTGCGGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTATTTATCCTGATTCTg < 1:1547988/63‑1 (MQ=255) gTTCCGTCTGCGGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTATTTATCCTGATTCTg < 1:2928518/63‑1 (MQ=255) gTTCCGTCTGCGGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTATTTATCCTGATTCTg < 1:3295937/63‑1 (MQ=255) gTTCCGTCTGCGGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTATTTATCCTGATTCTg < 1:1372253/63‑1 (MQ=255) gTTCCGTCTGCGGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTATTTATCCTGATTCTg < 1:2538358/63‑1 (MQ=255) cTGCGGACGTGAACGTAACGCTGTCTATGGCACTGGGCGt < 1:1688437/40‑1 (MQ=255) tGCGGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTATTTATCCTGATTCTGTTCTACAGCATCaa > 1:634568/1‑69 (MQ=255) tGCGGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTATTTATCCTGATTCTGTTCTACAGCATCaa > 1:1112329/1‑69 (MQ=255) tGCGGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTATTTATCCTGATTCTGTTCTACAGCATCaa > 1:521368/1‑69 (MQ=255) | TCGACCTGCTGCCGTACATTGCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCGGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTATTTATCCTGATTCTGTTCTACAGCATCAA > minE/2464710‑2464842 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |