| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,469,747 | C→T | 61.2% | D243D (GAC→GAT) | atpD → | F1 sector of membrane‑bound ATP synthase, beta subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,469,747 | 0 | C | T | 61.2% | 38.6 / 56.5 | 85 | D243D (GAC→GAT) | atpD | F1 sector of membrane‑bound ATP synthase, beta subunit |
| Reads supporting (aligned to +/- strand): ref base C (5/28); new base T (13/39); total (18/67) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.74e-01 | |||||||||||
Only 100 of 101 total aligned reads displayed.
GCTCTGACCGGTCTGACCATGGCTGAGAAATTCCGTGACGAAGGTCGTGACGTTCTGCTGTTCGTTGACAACATCTATCGTTACACCCTGGCCGGTACGGAAGTATCCGCACTGCTGGGCCG > minE/2469679‑2469800 | gCTCTGACCGGTCTGACCATGGCTGAGAAATTCCGTGACGAAGGTCGTGACGTTCTGCTGTTCGTTGac > 1:88449/1‑69 (MQ=255)gCTCTGACCGGTCTGACCATGGCTGAGAAATTCCGTGACGAAGGTCGTGACGTTCTGCTGTTCGTTGac > 1:745191/1‑69 (MQ=255)gCTCTGACCGGTCTGACCATGGCTGAGAAATTCCGTGACGAAGGTCGTGACGTTCTGCTGTTCGTTGac > 1:3361608/1‑69 (MQ=255)gCTCTGACCGGTCTGACCATGGCTGAGAAATTCCGTGACGAAGGTCGTGACGTTCTGCTGTTCGTTGac > 1:1502763/1‑69 (MQ=255) ctGACCGGTCTGACCATGGCTGAGAAATTCCGTGACGAAGGTCGTGACGTTCTGCTGTTCGTTGacaac > 1:1717562/1‑69 (MQ=255) ctGACCGGTCTGACCATGGCTGAGAAATTCCGTGACGAAGGTCGTGACGTTCTGCTGTTCGTTGacaac < 1:981960/69‑1 (MQ=255) ctGACCGGTCTGACCATGGCTGAGAAATTCCGTGACGAAGGTCGTGACGTTCTGCTGTTCGTTGacaac < 1:3044856/69‑1 (MQ=255) ctGACCGGTCTGACCATGGCTGAGAAATTCCGTGACGAAGGTCGTGACGTTCTGCTGTTCGTTGacaac > 1:641180/1‑69 (MQ=255) ctGACCGGTCTGACCATGGCTGAGAAATTCCGTGACGAAGGTCGTGACGTTCTGCTGTTCGTTGacaac < 1:1334634/69‑1 (MQ=255) ctGACCGGTCTGACCATGGCTGAGAAATTCCGTGACGAAGGTCGTGACGTTCTGCTGTTCGTTGacaac > 1:549957/1‑69 (MQ=255) ctGACCGGTCTGACCATGGCTGAGAAATTCCGTGACGAAGGTCGTGACGTTCTGCTGTTCGTTGacaac > 1:240338/1‑69 (MQ=255) ctGACCGGTCTGACCATGGCTGAGAAATTCCGTGACGAAGGTCGTGACGTTCTGCTGTTCGTTGacaac > 1:3280125/1‑69 (MQ=255) ctGACCGGTCTGACCATGGCTGAGAAATTCCGTGACGAAGGTCGTGACGTTCTGCTGTTCGTTGacaac < 1:1403505/69‑1 (MQ=255) ctGACCGGTCTGACCATGGCTGAGAAATTCCGTGACGAAGGTCGTGACGTTCTGCTGTTCGTTGacaac > 1:3333934/1‑69 (MQ=255) ctGACCGGTCTGACCATGGCTGAGAAATTCCGTGACGAAGGTCGTGACGTTCTGCTGTTCGTTGacaac > 1:3329624/1‑69 (MQ=255) ctGACCGGTCTGACCATGGCTGAGAAATTCCGTGACGAAGGTCGTGACGTTCTGCTGTTCGTTGacaa > 1:2583950/1‑68 (MQ=255) cGGTCTGACCATGGCTGAGAAATTCCGTGACGAAGGTCGTGACGTTCTGCTGTTCGTTGACAACATCt < 1:375680/68‑1 (MQ=255) ggCTGAGAAATTCCGTGACGAAGGTCGTGACGTTCTGCTGTTCGTTGACAACATCTATCGTTACACCCt > 1:1985016/1‑69 (MQ=255) ggCTGAGAAATTCCGTGACGAAGGTCGTGACGTTCTGCTGTTCGTTGACAACATCTATCGTTACACCCt > 1:1767826/1‑69 (MQ=255) gCTGAGAAATTCCGTGACGAATGTCGTGACGTATTGCTGTTCGTCGATAACATCTATCGTTACACCCTg < 1:2979075/69‑1 (MQ=255) gCTGAGAAATTCCGTGACGAAGGTCGTGACGTATTGCTGTTCGTCGATAACATCTATCGTTACACCCTg < 1:2334185/69‑1 (MQ=255) gCTGAGAAATTCCGTGACGAAGGTCGTGACGTATTGCTGTTCGTCGATAACATCTATCGTTACACCCTg < 1:2739489/69‑1 (MQ=255) gCTGAGAAATTCCGTGACGAAGGTCGTGACGTATTGCTGTTCGTCGATAACATCTATCGTTACACCCTg < 1:2000559/69‑1 (MQ=255) gCTGAGAAATTCCGTGACGAAGGTCGTGACGTATTGCTGTTCGTCGATAACATCTATCGTTACACCCTg < 1:2732760/69‑1 (MQ=255) gCTGAGAAATTCCGTGACGAAGGTCGTGACGTATTGCTGTTCGTCGATAACATCTATCGTTACACCCTg < 1:1054856/69‑1 (MQ=255) gCTGAGAAATTCCGTGACGAAGGTCGTGACGTATTGCTGTTCGTCGATAACATCTATCGTTACACCCTg < 1:3277840/69‑1 (MQ=255) gCTGAGAAATTCCGTGACGAAGGTCGTGACGTATTGCTGTTCGTCGATAACATCTATCGTTACACCCTg < 1:543997/69‑1 (MQ=255) gCTGAGAAATTCCGTGACGAAGGTCGTGACGTATTGCTGTTCGTCGATAACATCTATCGTTACACCCTg < 1:682411/69‑1 (MQ=255) gCTGAGAAATTCCGTGACGAAGGTCGTGACGTATTGCTGTTCGTCGATAACATCTATCGTTACACCCTg < 1:985662/69‑1 (MQ=255) gCTGAGAAATTCCGTGACGAAGGTCGTGACGTATTGCTGTTCGTCGATAACATCTATCGTTACACCCTg < 1:1162189/69‑1 (MQ=255) gCTGAGAAATTCCGTGACGAAGGTCGTGACGTATTGCTGTTCGTCGATAACATCTATCGTTACACCCTg < 1:171194/69‑1 (MQ=255) gCTGAGAAATTCCGTGACGAAGGTCGTGACGTATTGCTGTTCGTCGATAACATCTATCGTTACACCCTg < 1:1998157/69‑1 (MQ=255) gCTGAGAAATTCCGTGACGAAGGTCGTGACGTATTGCTGTTCGTCGATAACATCTATCGTTACACCCTg < 1:1337454/69‑1 (MQ=255) gCTGAGAAATTCCGTGACGAAGGTCGTGACGTATTGCTGTTCGTCGATAACATCTATCGTTACACCCTg < 1:1891726/69‑1 (MQ=255) gCTGAGAAATTCCGTGACGAAGGTCGTGACGTATTGCTGTTCGTCGATAACATCTATCGTTACACCCTg < 1:1863660/69‑1 (MQ=255) aaaTTCCGTGACGAATGTCGTGACGTTCTGCTGTTCGTTGACAACATCTATCGTTACACCCTGGCCgg < 1:3261/68‑1 (MQ=255) aaaTTCCGTGACGAAGGTCGTGACGTTCTGCTGTTCGTTGACAACATCTATCGTTACACCCTGGCCgg < 1:465869/68‑1 (MQ=255) aaaTTCCGTGACGAAGGTCGTGACGTTCTGCTGTTCGTTGACAACATCTATCGTTACACCCTGGCCgg < 1:1621892/68‑1 (MQ=255) aaaTTCCGTGACGAAGGTCGTGACGTTCTGCTGTTCGTTGACAACATCTATCGTTACACCCTGGCCgg < 1:2945464/68‑1 (MQ=255) aaaTTCCGTGACGAAGGTCGTGACGTTCTGCTGTTCGTTGACAACATCTATCGTTACACCCTGGCCgg < 1:2868553/68‑1 (MQ=255) aaaTTCCGTGACGAAGGTCGTGACGTTCTGCTGTTCGTTGACAACATCTATCGTTACACCCTGGCCgg < 1:1181971/68‑1 (MQ=255) aaaTTCCGTGACGAAGGTCGTGACGTTCTGCTGTTCGTTGACAACATCTATCGTTACACCCTGGCCgg < 1:1545250/68‑1 (MQ=255) aaaTTCCGTGACGAAGGTCGTGACGTTCTGCTGTTCGTTGACAACATCTATCGTTACACCCTGGCCgg < 1:3315120/68‑1 (MQ=255) aaaTTCCGTGACGAAGGTCGTGACGTTCTGCTGTTCGTTGACAACATCTATCGTTACACCCTGGCCgg < 1:2628350/68‑1 (MQ=255) aaaTTCCGTGACGAAGGTCGTGACGTTCTGCTGTTCGTTGACAACATCTATCGTTACACCCTGGCCgg < 1:244651/68‑1 (MQ=255) aaaTTCCGTGACGAAGGTCGTGACGTTCTGCTGTTCGTTGACAACATCTATCGTTACACCCTGGCCgg < 1:2438349/68‑1 (MQ=255) aaaTTCCGTGACGAAGGTCGTGACGTTCTGCTGTTCGTTGACAACATCTATCGTTACACCCTGGCCgg < 1:2397592/68‑1 (MQ=255) aaaTTCCGTGACGAAGGTCGTGACGTTCTGCTGTTCGTTGACAACATCTATCGTTACACCCTGGCCgg < 1:2007580/68‑1 (MQ=255) aaaTTCCGTGACGAAGGTCGTGACGTTCTGCTGTTCGTTGACAACATCTATCGTTACACCCTGGCCgg < 1:2459902/68‑1 (MQ=255) aaaTTCCGTGACGAAGGTCGTGACGTTCTGCTGTTCGTTGACAACATCTATCGTTACACCCTGGCCgg < 1:1150013/68‑1 (MQ=255) aaaTTCCGTGACGAAGGTCGTGACGTTCTGCTGTTCGTTGACAACATCTATCGTTACACCCTGGCCgg < 1:2130365/68‑1 (MQ=255) aaaTTCCGTGACGAAGGTCGTGACGTTCTGCTGTTCGTTGACAACATCTATCGTTACACCCTGGCCgg < 1:982531/68‑1 (MQ=255) aaaTTCCGTGACGAAGGTCGTGACGTTCTGCTGTTCGTTCACAACATCTATCGTTACACCCTGGCCgg < 1:2322310/68‑1 (MQ=255) aaTTCGGTGACGAAGGTCGTGACGTATTGCTGTTCGTCGATAACATCTATCGTTACACCCTg < 1:2225012/62‑1 (MQ=255) aaTTCCGTGACGAAGGTCGTGACGTATTGCTGTTCGTCGATAACATCTATCGTTACACCGTg < 1:3240632/62‑1 (MQ=255) aaTTCCGTGACGAAGGTCGTGACGTATTGCTGTTCGTCGATAACATCTATCGTTACACCCTg < 1:601387/62‑1 (MQ=255) aaTTCCGTGACGAAGGTCGTGACGTATTGCTGTTCGTCGATAACATCTATCGTTACACCCTg < 1:1356444/62‑1 (MQ=255) aaTTCCGTGACGAAGGTCGTGACGTATTGCTGTTCGTCGATAACATCTATCGTTACACCCTg < 1:3108674/62‑1 (MQ=255) aaTTCCGTGACGAAGGTCGTGACGTATTGCTGTTCGTCGATAACATCTATCGTTACACCCTg < 1:795524/62‑1 (MQ=255) aaTTCCGTGACGAAGGTCGTGACGTATTGCTGTTCGTCGATAACATCTATCGTTACACCCTg < 1:1403040/62‑1 (MQ=255) aaTTCCGTGACGAAGGTCGTGACGTATTGCTGTTCGTCGATAACATCTATCGTTACACCCTg < 1:661617/62‑1 (MQ=255) aaTTCCGTGACGAAGGTCGTGACGTATTGCTGTTCGTCGATAACATCTATCGTTACACCCTg < 1:131296/62‑1 (MQ=255) aaTTCCGTGACGAAGGTCGTGACGTATTGCTGTTCGTCGATAACATCTATCGTTACACCCTg < 1:3076378/62‑1 (MQ=255) aaTTCCGTGACGAAGGTCGTGACGTATTGCTGTTCGTCGATAACATCTATCGTTACACCCTg < 1:90831/62‑1 (MQ=255) aaTTCCGTGACGAAGGTCGTGACGTATTGCTGTTCGTCGATAACATCTATCGTTACACCCTg < 1:2866633/62‑1 (MQ=255) aaTTCCGTGACGAAGGTCGTGACGTATTGCTGTTCGTCGATAACATCTATCGTTACACCCTg < 1:1066713/62‑1 (MQ=255) aaTTCCGTGACGAAGGTCGTGACGTATTGCTGTTCGTCGATAACATCTATCGTTACACCCTg < 1:931549/62‑1 (MQ=255) aaTTCCGTGACGAAGGTCGTGACGTATTGCTGTTCGTCGATAACATCTATCGTTACACCCTg < 1:944802/62‑1 (MQ=255) aaTTCCGTGACGAAGGTCGTGACGTATTGCTGTTCGTCGATAACATCTATCGTTACACCCTg < 1:178380/62‑1 (MQ=255) aaTTCCGTGACGAAGGTCGTGACGTATTGCTGTTCGTCGATAACATCTATCGTTACACCCTg < 1:2739091/62‑1 (MQ=255) aaTTCCGTGACGAAGGTCGTGACGTATTGCTGTTCGTCGATAACATCTATCGTTACACCCTg < 1:2661365/62‑1 (MQ=255) aaTTCCGTGACGAAGGTCGTGACGTATTGCTGTTCGTCGATAACATCTATCGTTACACCCTg < 1:1872394/62‑1 (MQ=255) aaTTCCGTGACGAAGGTCGTGACGTATTGCTGTTCGTCGATAACATCTATCGTTACACCCTg < 1:1990866/62‑1 (MQ=255) aaTTCCGTGACGAAGGTCGTGACGTATTGCTGTTCGTCGATAACATCTATCGTTACACCCTg < 1:975968/62‑1 (MQ=255) aaTTCCGTGACGAAGGTCGTGACGTATTGCTGTTCGTCGATAACATCTATCGTTACACCCTg < 1:2137895/62‑1 (MQ=255) gTGACGTATTGCTGTTCGTCGATAACATCTATCGTTACACCCTGGCCGGTAcc > 1:665089/1‑52 (MQ=37) gTGACGTATTGCTGTTCGTCGATAACATCTATCGTTACACCCTGGCCGGTACCGAAGTATCCGCActgc > 1:3195414/1‑69 (MQ=255) gTGACGTATTGCTGTTCGTCGATAACATCTATCGTTACACCCTGGCCGGTACCGAAGTATCCGCActgc > 1:1289022/1‑69 (MQ=255) gTGACGTATTGCTGTTCGTCGATAACATCTATCGTTACACCCTGGCCGGTACCGAAGTATCCGCActgc > 1:8883/1‑69 (MQ=255) gTGACGTATTGCTGTTCGTCGATAACATCTATCGTTACACCCTGGCCGGTACCGAAGTATCCGCActgc > 1:3215826/1‑69 (MQ=255) gTGACGTATTGCTGTTCGTCGATAACATCTATCGTTACACCCTGGCCGGTACCGAAGTATCCGCActgc > 1:2004553/1‑69 (MQ=255) gTGACGTATTGCTGTTCGTCGATAACATCTATCGTTACACCCTGGCCGGTACCGAAGTATCCGCActgc > 1:2101761/1‑69 (MQ=255) gTGACGTATTGCTGTTCGTCGATAACATCTATCGTTACACCCTGGCCGGTACCGAAGTATCCGCActgc > 1:2286865/1‑69 (MQ=255) gTGACGTATTGCTGTTCGTCGATAACATCTATCGTTACACCCTGGCCGGTACCGAAGTATCCGCActgc > 1:2295692/1‑69 (MQ=255) gTGACGTATTGCTGTTCGTCGATAACATCTATCGTTACACCCTGGCCGGTACCGAAGTATCCGCActgc > 1:1559150/1‑69 (MQ=255) gTGACGTATTGCTGTTCGTCGATAACATCTATCGTTACACCCTGGCCGGTACCGAAGTATCCGCActgc > 1:1500287/1‑69 (MQ=255) gTGACGTATTGCTGTTCGTCGATAACATCTATCGTTACACCCTGGCCGGTACCGAAGTATCCGCActgc > 1:358878/1‑69 (MQ=255) gTGACGTATTGCTGTTCGTCGATAACATCTATCGTTACACCCTGGCCGGTACCGAAGTATCCGCActgc > 1:1364900/1‑69 (MQ=255) aCGTTCTGCTGTTCGTTGACAACATCTATCGTTACACCCTGGCCGGTACGGAAGTATCCGCACTGCTgg > 1:3222360/1‑69 (MQ=255) aCGTTCTGCTGTTCGTTGACAACATCTATCGTTACACCCTGGCCGGTACGGAAGTATCCGCACTGCTgg > 1:2978763/1‑69 (MQ=255) aCGTTCTGCTGTTCGTTGACAACATCTATCGTTACACCCTGGCCGGTACGGAAGTATCCGCACTGCTgg > 1:552004/1‑69 (MQ=255) tCTGCTGTTCGTTGACAACATCTATCGTTACACCCTGGCCGGTACGGAAGTATCCGCACTGCTGGGcct < 1:516584/69‑2 (MQ=255) tCTGCTGTTCGTTGACAACATCTATCGTTACACCCTGGCCGGTACGGAAGTATCCGCACTGCTGGGCCg < 1:648357/69‑1 (MQ=255) tCTGCTGTTCGTTGACAACATCTATCGTTACACCCTGGCCGGTACGGAAGTATCCGCACTGCTGGGCCg < 1:1481642/69‑1 (MQ=255) tCTGCTGTTCGTTGACAACATCTATCGTTACACCCTGGCCGGTACGGAAGTATCCGCACTGCTGGGCCg < 1:3182365/69‑1 (MQ=255) tCTGCTGTTCGTTGACAACATCTATCGTTACACCCTGGCCGGTACGGAAGTATCCGCACTGCTGGGCCg < 1:3081260/69‑1 (MQ=255) tCTGCTGTTCGTTGACAACATCTATCGTTACACCCTGGCCGGTACGGAAGTATCCGCACTGCTGGGCCg < 1:2980224/69‑1 (MQ=255) tCTGCTGTTCGTTGACAACATCTATCGTTACACCCTGGCCGGTACGGAAGTATCCGCACTGCTGGGCCg < 1:2836560/69‑1 (MQ=255) tCTGCTGTTCGTTGACAACATCTATCGTTACACCCTGGCCGGTACGGAAGTATCCGCACTGCTGGGCCg < 1:491387/69‑1 (MQ=255) ctgctgTTCGTTGACAACATCTATCGTTACACCCTGGCCgg < 1:284579/41‑1 (MQ=255) | GCTCTGACCGGTCTGACCATGGCTGAGAAATTCCGTGACGAAGGTCGTGACGTTCTGCTGTTCGTTGACAACATCTATCGTTACACCCTGGCCGGTACGGAAGTATCCGCACTGCTGGGCCG > minE/2469679‑2469800 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |