| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,472,750 | G→A | 8.7% | A9T (GCG→ACG) | glmS → | L‑glutamine:D‑fructose‑6‑phosphate aminotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,472,750 | 0 | G | A | 8.7% | 328.3 / 10.5 | 156 | A9T (GCG→ACG) | glmS | L‑glutamine:D‑fructose‑6‑phosphate aminotransferase |
| Reads supporting (aligned to +/- strand): ref base G (62/80); new base A (3/11); total (65/91) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.56e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.37e-01 | |||||||||||
Only 100 of 159 total aligned reads displayed.
CAGGTTGACCGACAACGATATAAATCGGAATCAAAAACTATGTGTGGAATTGTTGGCGCGATCGCGCAACGTGATGTAGCAGAAATCCTTCTTGAAGGTTTACGTCGTCTGGAATACCGCGGA > minE/2472687‑2472809 | cAGGTTGACCGACAACGATATAAATCGGAATCAAAAACTATGTGTGGAATTGTTGGCGCGATCGCGCaa > 1:1023883/1‑69 (MQ=255)cAGGTTGACCGACAACGATATAAATCGGAATCAAAAACTATGTGTGGAATTGTTGGCGCGATCGCGCaa > 1:983167/1‑69 (MQ=255)cAGGTTGACCGACAACGATATAAATCGGAATCAAAAACTATGTGTGGAATTGTTGGCGCGATCGCGCaa > 1:776071/1‑69 (MQ=255)cAGGTTGACCGACAACGATATAAATCGGAATCAAAAACTATGTGTGGAATTGTTGGCGCGATCGCGCaa > 1:684663/1‑69 (MQ=255)cAGGTTGACCGACAACGATATAAATCGGAATCAAAAACTATGTGTGGAATTGTTGGCGCGATCGCGCaa > 1:3292323/1‑69 (MQ=255)cAGGTTGACCGACAACGATATAAATCGGAATCAAAAACTATGTGTGGAATTGTTGGCGCGATCGCGCaa > 1:3078604/1‑69 (MQ=255)cAGGTTGACCGACAACGATATAAATCGGAATCAAAAACTATGTGTGGAATTGTTGGCGCGATCGCGCaa > 1:2823269/1‑69 (MQ=255)cAGGTTGACCGACAACGATATAAATCGGAATCAAAAACTATGTGTGGAATTGTTGGCGCGATCGCGCaa > 1:2813729/1‑69 (MQ=255)cAGGTTGACCGACAACGATATAAATCGGAATCAAAAACTATGTGTGGAATTGTTGGCGCGATCGCGCaa > 1:2608703/1‑69 (MQ=255)cAGGTTGACCGACAACGATATAAATCGGAATCAAAAACTATGTGTGGAATTGTTGGCGCGATCGCGCaa > 1:2575130/1‑69 (MQ=255)cAGGTTGACCGACAACGATATAAATCGGAATCAAAAACTATGTGTGGAATTGTTGGCGCGATCGCGCaa > 1:2451012/1‑69 (MQ=255)cAGGTTGACCGACAACGATATAAATCGGAATCAAAAACTATGTGTGGAATTGTTGGCGCGATCGCGCaa > 1:2362243/1‑69 (MQ=255)cAGGTTGACCGACAACGATATAAATCGGAATCAAAAACTATGTGTGGAATTGTTGGCGCGATCGCGCaa > 1:2316941/1‑69 (MQ=255)cAGGTTGACCGACAACGATATAAATCGGAATCAAAAACTATGTGTGGAATTGTTGGCGCGATCGCGCaa > 1:1801611/1‑69 (MQ=255)cAGGTTGACCGACAACGATATAAATCGGAATCAAAAACTATGTGTGGAATTGTTGGCGCGATCGCGCaa > 1:1792186/1‑69 (MQ=255)cAGGTTGACCGACAACGATATAAATCGGAATCAAAAACTATGTGTGGAATTGTTGGCGCGATCGCGCaa > 1:1544704/1‑69 (MQ=255)cAGGTTGACCGACAACGATATAAATCGGAATCAAAAACTATGTGTGGAATTGTTGGCGCGATCGCGCaa > 1:1088437/1‑69 (MQ=255)cAGGTTGACCGACAACGATATAAATCGGAATCAAAAACTATGTGTGGAATTGTTGGCGCGATCGCGCa > 1:829970/1‑68 (MQ=255)cAGGTTGACCGACAACGATATAAATCGGAATCAAAAACTATGTGTGGAATTGTTGGCGCGATCGCGCa > 1:517281/1‑68 (MQ=255)cAGGTTGACCGACAACGATATAAATCGGAATCAAAAACTATGTGTGGAATTGTTGGCGCGATCGCGCa > 1:427897/1‑68 (MQ=255)cAGGTTGACCGACAACGATATAAATCGGAATCAAAAACTATGTGTGGAATTGTTGGCGCGATCGCGCa > 1:350377/1‑68 (MQ=255)cAGGTTGACCGACAACGATATAAATCGGAATCAAAAACTATGTGTGGAATAGTTGGCGCGATCGCGCaa > 1:2145694/1‑69 (MQ=255) gTTGACCGACAACGATATAAATCGGAATCAAAAACTATGTGTGGAATTGTTGGCGCGATCGCGCAACGt < 1:956509/69‑1 (MQ=255) gTTGACCGACAACGATATAAATCGGAATCAAAAACTATGTGTGGAATTGTTGGCGCGATCGCGCAACGt < 1:2337005/69‑1 (MQ=255) gTTGACCGACAACGATATAAATCGGAAT‑AAAAACTATGTGTGGAATTGTTGGCGCGATCGCGCAACGTg < 1:1615095/69‑1 (MQ=255) ttGACCGACAACGATATAAATCGGAATCAAAAACTATGTGTGGAATTGTTGGCGCGATCGCGCAACGt < 1:1478897/68‑1 (MQ=255) ttGACCGACAACGATATAAATCGGAATCAAAAACTATGTGTGGAATTGTTGGCGCGATCGCGCAACGTg < 1:3254931/69‑1 (MQ=255) ttGACCGACAACGATATAAATCGGAATCAAAAACTATGTGTGGAATTGTTGGCGCGATCGCGCAACGTg < 1:2649380/69‑1 (MQ=255) ttGACCGACAACGATATAAATCGGAATCAAAAACTATGTGTGGAATTGTTGGCGCGATCGCGCAACGTg < 1:2701563/69‑1 (MQ=255) ttGACCGACAACGATATAAATCGGAATCAAAAACTATGTGTGGAATTGTTGGCGCGATCGCGCAACGTg < 1:2058006/69‑1 (MQ=255) ttGACCGACAACGATATAAATCGGAATCAAAAACTATGTGTGGAATTGTTGGCGCGATCGCGCAACGTg < 1:2928724/69‑1 (MQ=255) ttGACCGACAACGATATAAATCGGAATCAAAAACTATGTGTGGAATTGTTGGCGCGATCGCGCAACGTg < 1:3208436/69‑1 (MQ=255) ttGACCGACAACGATATAAATCGGAATCAAAAACTATGTGTGGAATTGTTGGCGCGATCGCGCAACGTg < 1:1848631/69‑1 (MQ=255) ttGACCGACAACGATATAAATCGGAATCAAAAACTATGTGTGGAATTGTTGGCGCGATCGCGCAACGTg < 1:3274227/69‑1 (MQ=255) ttGACCGACAACGATATAAATCGGAATCAAAAACTATGTGTGGAATTGTTGGCGCGATCGCGCAACGTg < 1:3347040/69‑1 (MQ=255) ttGACCGACAACGATATAAATCGGAATCAAAAACTATGTGTGGAATTGTTGGCGCGATCGCGCAACGTg < 1:711097/69‑1 (MQ=255) ttGACCGACAACGATATAAATCGGAATCAAAAACTATGTGTGGAATTGTTGGCGCGATCGCGCAACGTg < 1:1569904/69‑1 (MQ=255) tGACCGACAACGATATAAATCGGAATCAAAAACTATGTGTGGAATTGTTGGCGCGATCGCGCAACGTg < 1:2628570/68‑1 (MQ=255) tGACCGACAACGATATAAATCGGAATCAAAAACTATGTGTGGAATTGTTGGCGCGATCGCGCAACGTGa > 1:2929126/1‑69 (MQ=255) cGACAACGATATAAATCGGAATCAAAAACTATGTGTGGAATTGTTGGCGCGATCGCGCAATGTGATGt < 1:1344568/68‑1 (MQ=255) cGACAACGATATAAATCGGAATCAAAAACTATGTGTGGAATTGTTGGCGCGATCGCGCAACGTGATGt < 1:1022325/68‑1 (MQ=255) cGACAACGATATAAATCGGAATCAAAAACTATGTGTGGAATTGTTGGCGCGATCGCGCAACGTGATGt < 1:754503/68‑1 (MQ=255) cGACAACGATATAAATCGGAATCAAAAACTATGTGTGGAATTGTTGGCGCGATCGCGCAACGTGATGt < 1:2661054/68‑1 (MQ=255) cGACAACGATATAAATCGGAATCAAAAACTATGTGTGGAATTGTTGGCGCGATCGCGCAACGTGATGt < 1:914667/68‑1 (MQ=255) atatAAATCGGAATCAAAAACTATGTGTGGAATTGTTGGCGCGATCACGCAACGTGATGTAGCAGAAAt > 1:1298578/1‑69 (MQ=255) atatAAATCGGAATCAAAAACTATGTGTGGAATTGTTGGCGCGATCACGCAACGTGATGTAGCAGAAAt > 1:3282753/1‑69 (MQ=255) atatAAATCGGAATCAAAAACTATGTGTGGAATTGTTGGCGCGATCACGCAACGTGATGTAGCAGAAAt > 1:2626117/1‑69 (MQ=255) ataAATCGGAATCAAAAACTATGTGTGGAATTGTTGGCGCGATCGCGCAACGTGATGTAGCAGAAATcc < 1:288196/69‑1 (MQ=255) ataAATCGGAATCAAAAACTATGTGTGGAATTGTTGGCGCGATCGCGCAACGTGATGTAGCAGAAATcc < 1:474362/69‑1 (MQ=255) ataAATCGGAATCAAAAACTATGTGTGGAATTGTTGGCGCGATCGCGCAACGTGATGTAGCAGAAATcc < 1:2798318/69‑1 (MQ=255) ataAATCGGAATCAAAAACTATGTGTGGAATTGTTGGCGCGATCGCGCAACGTGATGTAGCAGAAATcc < 1:1045716/69‑1 (MQ=255) ataAATCGGAATCAAAAACTATGTGTGGAATTGTTGGCGCGATCGCGCAACGTGATGTAGCAGAAATcc < 1:1245466/69‑1 (MQ=255) taAATCGGAATCAAAAACTATGTGTGGAATTGTTGGCGCGATCGCGCAACGTGATGTAGCAGAAATcc < 1:1698213/68‑1 (MQ=255) aaaaaCTATGTGTGGAATTGTTGGCGCGATCGCGCa > 1:1265404/1‑36 (MQ=255) aaaaaCTATGTGTGGAATTGTTGGCGCGATCGCGCAACGTGATGTAGCAGAAATCCTTCTTGAAGGttt > 1:2359208/1‑69 (MQ=255) aaaaaCTATGTGTGGAATTGTTGGCGCGATCGCGCAACGTGATGTAGCAGAAATCCTTCTTGAAGGttt > 1:1638447/1‑69 (MQ=255) aaaaaCTATGTGTGGAATTGTTGGCGCGATCGCGCAACGTGATGTAGCAGAAATCCTTCTTGAAGGttt > 1:1471749/1‑69 (MQ=255) aaaaaCTATGTGTGGAATTGTTGGCGCGATCGCGCAACGTGATGTAGCAGAAATCCTTCTTGAAGGttt > 1:1752297/1‑69 (MQ=255) aaaaaCTATGTGTGGAATTGTTGGCGCGATCGCGCAACGTGATGTAGCAGAAATCCTTCTTGAAGGttt > 1:2786852/1‑69 (MQ=255) aaaaaCTATGTGTGGAATTGTTGGCGCGATCGCGCAACGTGATGTAGCAGAAATCCTTCTTGAAGGtt > 1:310983/1‑68 (MQ=255) aaaaaCTATGTGTGGAATTGTTGGCGCGATCGCGCAACGTGATGTAGCAGAAATCCTTCTTGAAGGtt > 1:370278/1‑68 (MQ=255) aaaaaCTATGTGTGGAATTGTTGGCGCGATCGCGCAACGTGATGTAGCAGAAATCCTTCTTGAAGGtt < 1:1389252/68‑1 (MQ=255) aaaaaCTATGTGTGGAATTGTTGGCGCGATCGCGCAACGTGATGTAGCAGAAATCCTTCTTGAAGGtt < 1:2209209/68‑1 (MQ=255) aaaaaCTATGTGTGGAATTGTTGGCGCGATCGCGCAACGTGATGTAGCAGAAATCCTTCTTGAAGGt > 1:3171556/1‑67 (MQ=255) aaaaaCTATGTGTGGAATTGTTGGCGCGATCGCGCAACGTGATGTAGCAGAAATCCCTCTTGAAGGttt > 1:1046636/1‑69 (MQ=255) cTATGTGTGGAATTGTTGGCGCGATCGCGCAACGTGATGTAGCAGAAATCCTTCTTGAAGGTTTAcgtc > 1:848219/1‑69 (MQ=255) cTATGTGTGGAATTGTTGGCGCGATCGCGCAACGTGATGTAGCAGAAATCCTTCTTGAAGGTTTAcgtc > 1:2736400/1‑69 (MQ=255) cTATGTGTGGAATTGTTGGCGCGATCGCGCAACGTGATGTAGCAGAAATCCTTCTTGAAGGTTTAcgt > 1:194932/1‑68 (MQ=255) tgtgGAATTGTTGGCGCGATCGCGCAACGTGATGTAGCAGAAATCCTTCTTGAAGGTTTACgtcgtc > 1:71512/1‑67 (MQ=255) tgtgGAATTGTTGGCGCGATCGCGCAACGTGATGTAGCAGAAATCCTTCTTGAAGGTTTACGTCGTCt > 1:488302/1‑68 (MQ=255) tgtgGAATTGTTGGCGCGATCGCGCAACGTGATGTAGCAGAAATCCTTCTTGAAGGTTTACGTCGTCt > 1:143952/1‑68 (MQ=255) tgGAATTGTTGGCGCGATCGCGCAACGTGATGTAGCAGAAATCCTTCTTGAAGGTTTACGTCGTCTgg < 1:2375999/68‑1 (MQ=255) tgGAATTGTTGGCGCGATCGCGCAACGTGATGTAGCAGAAATCCTTCTTGAAGGTTTACGTCGTCTgg < 1:2501099/68‑1 (MQ=255) tgGAATTGTTGGCGCGATCGCGCAACGTGATGTAGCAGAAATCCTTCTTGAAGGTTTACGTCGTCTgg < 1:2505196/68‑1 (MQ=255) tgGAATTGTTGGCGCGATCGCGCAACGTGATGTAGCAGAAATCCTTCTTGAAGGTTTACGTCGTCTgg < 1:2553770/68‑1 (MQ=255) tgGAATTGTTGGCGCGATCGCGCAACGTGATGTAGCAGAAATCCTTCTTGAAGGTTTACGTCGTCTgg < 1:2633561/68‑1 (MQ=255) tgGAATTGTTGGCGCGATCGCGCAACGTGATGTAGCAGAAATCCTTCTTGAAGGTTTACGTCGTCTgg < 1:1376520/68‑1 (MQ=255) gAATTGTTGGCGCGATCGCGCAACGTGATGTAGCAGAAATCCTTCTTGAAGGTTTACGTCGTCTGGaa < 1:1195896/68‑1 (MQ=255) tgGCGCGATCGCGCAACGTGATGTAGCAGAAATCCTTCTTGAAGGTTTACGTCGTCTGGAATACcgcg < 1:3005023/68‑1 (MQ=255) tgGCGCGATCGCGCAACGTGATGTAGCAGAAATCCTTCTTGAAGGTTTACGTCGTCTGGAATACcgcg < 1:1646042/68‑1 (MQ=255) tgGCGCGATCGCGCAACGTGATGTAGCAGAAATCCTTCTTGAAGGTTTACGTCGTCTGGAATACcgcg < 1:1959588/68‑1 (MQ=255) ggCGCGATCGCGCAACGTGATGTAGCAGAAATCCTTCTTGAAGGTTTACGTCGTCTGGAATACCGCgg < 1:2437177/68‑1 (MQ=255) ggCGCGATCGCGCAACGTGATGTAGCAGAAATCCTTCTTGAAGGTTTACGTCGTCTGGAATACCGCgg < 1:1357141/68‑1 (MQ=255) ggCGCGATCGCGCAACGTGATGTAGCAGAAATCCTTCTTGAAGGTTTACGTCGTCTGGAATACCGCgg < 1:1188944/68‑1 (MQ=255) ggCGCGATCGCGCAACGTGATGTAGCAGAAATCCTTCTTGAAGGTTTACGTCGTCTGGAATACCGCgg < 1:3365718/68‑1 (MQ=255) ggCGCGATCGCGCAACGTGATGTAGCAGAAATCCTTCTTGAAGGTTTACGTCGTCTGGAATACCGCgg < 1:2329178/68‑1 (MQ=255) ggCGCGATCGCGCAACGTGATGTAGCAGAAATCCTTCTTGAAGGTTTACGTCGTCTGGAATACCGCGGa < 1:1937459/69‑1 (MQ=255) ggCGCGATCGCGCAACGTGATGTAGCAGAAATCCTTCTTGAAGGTTTACGTCGTCTGGAATACCGCGGa < 1:927613/69‑1 (MQ=255) ggCGCGATCGCGCAACGTGATGTAGCAGAAATCCTTCTTGAAGGTTTACGTCGTCTGGAATACCGCGGa < 1:1612121/69‑1 (MQ=255) ggCGCGATCACGCAACGTGATGTAGCAGAAATCCTTCTTGAAGGTTTACGTCGTCTGGAATACCGCGGa < 1:628313/69‑1 (MQ=255) ggCGCGATCACGCAACGTGATGTAGCAGAAATCCTTCTTGAAGGTTTACGTCGTCTGGAATACCGCGGa < 1:610712/69‑1 (MQ=255) ggCGCGATCACGCAACGTGATGTAGCAGAAATCCTTCTTGAAGGTTTACGTCGTCTGGAATACCGCGGa < 1:3001854/69‑1 (MQ=255) ggCGCGATCACGCAACGTGATGTAGCAGAAATCCTTCTTGAAGGTTTACGTCGTCTGGAATACCGCGGa < 1:1800763/69‑1 (MQ=255) ggCGCGATCACGCAACGTGATGTAGCAGAAATCCTTCTTGAAGGTTTACGTCGTCTGGAATACCGCGGa < 1:422338/69‑1 (MQ=255) ggCGCGATCACGCAACGTGATGTAGCAGAAATCCTTCTTGAAGGTTTACGTCGTCTGGAATACCGCGGa < 1:2957802/69‑1 (MQ=255) ggCGCGAGCGCGCAACGTGATGTAGCAGAAATCCTTCTTGAAGGTTTACGTCGTCTGGAATACCGCGGa < 1:1136616/69‑1 (MQ=255) gcgcGATCGCGCAACGTGATGTAGCAGAAATCCTTCTTGAAGGTTTACGTCGTCTGGAATACCGCGGa < 1:1849832/68‑1 (MQ=255) aTCGCGCAACGTGATGTAGCAGAAATCCTTCTTGAAGGTTTACGTCGTCTGGAATACCGCGGa < 1:1078760/63‑1 (MQ=255) cgcgCAACGTGATGTAGCAGAAATCCTTCTTGAAGGTTTACGTCGTCTGGAATACCGCGGa > 1:2240081/1‑61 (MQ=255) cgcgCAACGTGATCTAGCAGAAATCCTTCTTGAAGGTTTAcgtc > 1:1752935/1‑44 (MQ=255) | CAGGTTGACCGACAACGATATAAATCGGAATCAAAAACTATGTGTGGAATTGTTGGCGCGATCGCGCAACGTGATGTAGCAGAAATCCTTCTTGAAGGTTTACGTCGTCTGGAATACCGCGGA > minE/2472687‑2472809 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |