| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 209,334 | C→T | 39.9% | L124L (TTG→TTA) | yafC ← | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 209,334 | 0 | C | T | 39.9% | 9.7 / 16.7 | 30 | L124L (TTG→TTA) | yafC | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (4/14); new base T (7/5); total (11/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.26e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.91e-01 | |||||||||||
AGCGCGTATCGCGACATCCACTTTTCTTTCGATCAAATTAATAATCGTTTCGGAGGAGACTAGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCATCAGAAAGTGCAGCACC > minE/209268‑209397 | aGCGCGTATCGCGACATCCACTTTTCTTTCGATCAAATTAATAATCGTTTCGGAGGAGACTAGCGACaa < 1:54558/69‑1 (MQ=255)aGCGCGTATCGCGACATCCACTTTTCTTTCGATCAAATTAATAATCGTTTCGGAGGAGACTAGCGACaa < 1:461245/69‑1 (MQ=255)aGCGCGTATCGCGACATCCACTTTTCTTTCGATCAAATTAATAATCGTTTCGGAGGAGACTAGCGACaa < 1:3367180/69‑1 (MQ=255)aGCGCGTATCGCGACATCCACTTTTCTTTCGATCAAATTAATAATCGTTTCGGAGGAGACTAGCGACaa < 1:3103237/69‑1 (MQ=255) gcgACATCCACTTTTCTTTCGATCAAATTAATAATCGTTTCGGAGGAGACTAGCGACAAAGTGACTTc > 1:283823/1‑68 (MQ=255) gcgACATCCACTTTTCTTTCGATCAAATTAATAATCGTTTCGGAGGAGACTAGCGACAAAGTGACTTc > 1:2530767/1‑68 (MQ=255) cACTTTTCTTTCGATCAAATTAATAATCGTTTCGGAGGAGACTAGCGACAAAGTg > 1:1844789/1‑55 (MQ=255) ttctttcGATCAAATTAATAATCGTTTCGGAGGAGACTAGCGACAAAGTGACTTCCGGATAGCGtt > 1:806924/1‑66 (MQ=255) aTCAAATTAATAATCGTTTCGGAGGAGACTAGCGACaa < 1:477168/38‑1 (MQ=255) cAAATTAATAATCGTTTCGGAGGAGACTAGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCtt < 1:1563531/69‑1 (MQ=255) aTTAATAATCGTTTCGGAGGAGACTAGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCttaa < 1:940938/68‑1 (MQ=255) aTTAATAATCGTTTCGGAGGAGACTAGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCttaa < 1:1702679/68‑1 (MQ=255) ttAATAATCGTTTCGGAGGAGACTAGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCttaatt < 1:1163118/69‑1 (MQ=255) taatCGTTTCGGAGGAGACTAGCGATAAAGTGACTTCCGGATAGCGTTCACGAAAAGGCTTAATCAAc < 1:1152451/68‑1 (MQ=255) aatCGTTTCGGAGGAGACTAGCGATAAAGTGACTTCCGGATAGCGTTCACGAAAAGGCTTAATCAAc < 1:1013566/67‑1 (MQ=255) aatCGTTTCGGAGGAGACTAGCGATAAAGTGACTTCCGGATAGCGTTCACGAAAAGGCTTAATCAAc < 1:2634719/67‑1 (MQ=255) aatCGTTTCGGAGGAGACTAGCGATAAAGTGACTTCCGGATAGCGTTCACGAAAAGGCTTAATCAAc < 1:654568/67‑1 (MQ=255) aatCGTTTCGGAGGAGACTAGCGATAAAGTGACTTCCGGATAGCGTTCACGAAAAGGCTTAATCAAc < 1:377306/67‑1 (MQ=255) ggaggaGACTAGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCttaa < 1:343179/53‑1 (MQ=255) gaCTAGCGATAAAGTGACTTCCGGATAGCGTTCAc > 1:2405517/1‑35 (MQ=255) gaCTAGCGATAAAGTGACTTCCGGATAGCGTTCACGAAAAGGCTTAATCAACGGCATCAGAAAGTgcag > 1:1229329/1‑69 (MQ=255) gaCTAGCGATAAAGTGACTTCCGGATAGCGTTCACGAAAAGGCTTAATCAACGGCATCAGAAAGTgcag > 1:3212568/1‑69 (MQ=255) gaCTAGCGATAAAGTGACTTCCGGATAGCGTTCACGAAAAGGCTTAATCAACGGCATCAGAAAGTgcag > 1:1187523/1‑69 (MQ=255) gaCTAGCGATAAAGTGACTTCCGGATAGCGTTCACGAAAAGGCTTAATCAACGGCATCAGAAAGTgcag > 1:26496/1‑69 (MQ=255) gaCTAGCGATAAAGTGACTTCCGGATAGCGTTCACGAAAAGGCTTAATCAACGGCATCAGAAAGTgca > 1:1333605/1‑68 (MQ=255) gaCTAGCGATAAAGTGACTTCCGGATAGCGTTCACGAAAAGGCTTAATCAACGGCATCAGAAAGTgca > 1:1040679/1‑68 (MQ=255) aGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCATCAGAAAGTGCAGcacc < 1:410285/69‑1 (MQ=255) aGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCATCAGAAAGTGCAGcacc < 1:311030/69‑1 (MQ=255) aGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCATCAGAAAGTGCAGcacc < 1:936930/69‑1 (MQ=255) aGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCATCAGAAAGTGCAGcacc < 1:2220082/69‑1 (MQ=255) | AGCGCGTATCGCGACATCCACTTTTCTTTCGATCAAATTAATAATCGTTTCGGAGGAGACTAGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCATCAGAAAGTGCAGCACC > minE/209268‑209397 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |