Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,474,447 G→A 31.9% P574P (CCG→CCA glmS → L‑glutamine:D‑fructose‑6‑phosphate aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,474,4470GA31.9% 117.5 / 66.4 131P574P (CCG→CCAglmSL‑glutamine:D‑fructose‑6‑phosphate aminotransferase
Reads supporting (aligned to +/- strand):  ref base G (63/26);  new base A (30/12);  total (93/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.19e-01

Only 100 of 138 total aligned reads displayed.

TTTGTAAGTAGCGATAACATGCACATCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCACCGACG  >  minE/2474385‑2474511
                                                              |                                                                
tttGTAAGTAGCGATAACATGCACATCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGAtcttc                                                            <  1:2058402/69‑1 (MQ=255)
tttGTAAGTAGCGATAACATGCACATCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGAtcttc                                                            <  1:2132992/69‑1 (MQ=255)
tttGTAAGTAGCGATAACATGCACATCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGAtcttc                                                            <  1:3343264/69‑1 (MQ=255)
tttGTAAGTAGCGATAACATGCACATCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGAtcttc                                                            <  1:2020501/69‑1 (MQ=255)
 ttGTAAGTAGCGATAACATGCACATCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCg                                                                  <  1:672084/62‑1 (MQ=255)
 ttGTAAGTAGCGATAACATGCACATCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCg                                                                  <  1:421010/62‑1 (MQ=255)
 ttGTAAGTAGCGATAACATGCACATCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCg                                                                  <  1:820036/62‑1 (MQ=255)
 ttGTAAGTAGCGATAACATGCACATCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCg                                                                  <  1:2955445/62‑1 (MQ=255)
 ttGTAAGTAGCGATAACATGCACATCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCg                                                                  <  1:135430/62‑1 (MQ=255)
          gCGATAACATGCACATCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTc                                                  >  1:1142332/1‑69 (MQ=255)
          gCGATAACATGCACATCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTc                                                  >  1:2781002/1‑69 (MQ=255)
          gCGATAACATGCACATCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTc                                                  >  1:1944876/1‑69 (MQ=255)
                   tGCACATCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCa                                           >  1:1057484/1‑67 (MQ=255)
                   tGCACATCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAgc                                         >  1:812586/1‑69 (MQ=255)
                   tGCACATCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAgc                                         >  1:635682/1‑69 (MQ=255)
                   tGCACATCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAgc                                         >  1:3175942/1‑69 (MQ=255)
                   tGCACATCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAgc                                         >  1:1059259/1‑69 (MQ=255)
                   tGCACATCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAgc                                         >  1:2375039/1‑69 (MQ=255)
                     cacaTCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAgctg                                       >  1:1776186/1‑69 (MQ=255)
                     cacaTCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAgctg                                       >  1:1925997/1‑69 (MQ=255)
                     cacaTCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAgctg                                       >  1:1529782/1‑69 (MQ=255)
                     cacaTCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAgctg                                       >  1:1677906/1‑69 (MQ=255)
                     cacaTCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAgctg                                       >  1:2579290/1‑69 (MQ=255)
                     cacaTCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAgctg                                       >  1:925708/1‑69 (MQ=255)
                     cacaTCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAgctg                                       >  1:2934127/1‑69 (MQ=255)
                     cacaTCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAgctg                                       >  1:343929/1‑69 (MQ=255)
                     cacaTCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAgctg                                       >  1:1338498/1‑69 (MQ=255)
                     cacaTCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAgctg                                       >  1:969507/1‑69 (MQ=255)
                     cacaTCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAgctg                                       >  1:3339681/1‑69 (MQ=255)
                     cacaTCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAgct                                        >  1:1038286/1‑68 (MQ=255)
                         tcatcGAGATGCCGCATGTGGAAGAGGTTATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTgg                                   >  1:1768700/1‑69 (MQ=255)
                           atcGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCt                                 >  1:2040144/1‑69 (MQ=255)
                           atcGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCt                                 >  1:978065/1‑69 (MQ=255)
                           atcGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCt                                 >  1:576787/1‑69 (MQ=255)
                           atcGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCt                                 >  1:2525098/1‑69 (MQ=255)
                           atcGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCt                                 >  1:3104141/1‑69 (MQ=255)
                           atcGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCt                                 >  1:3203136/1‑69 (MQ=255)
                           atcGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCt                                 >  1:1355912/1‑69 (MQ=255)
                           atcGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCt                                 >  1:1549987/1‑69 (MQ=255)
                           atcGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCt                                 >  1:822839/1‑69 (MQ=255)
                           atcGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCAGCTGGCt                                 >  1:541948/1‑69 (MQ=255)
                             cGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCTCTGCAGCTGCTGGCtt                                <  1:1233775/68‑1 (MQ=255)
                             cGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCtt                                <  1:3265696/68‑1 (MQ=255)
                             cGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCtt                                >  1:2816408/1‑68 (MQ=255)
                             cGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCtt                                <  1:540319/68‑1 (MQ=255)
                             cGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCtt                                <  1:1012889/68‑1 (MQ=255)
                             cGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCtt                                <  1:681900/68‑1 (MQ=255)
                             cGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCt                                 >  1:3176485/1‑67 (MQ=255)
                             cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCTACTACTGGCtt                                <  1:2023679/68‑1 (MQ=255)
                             cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCtt                                <  1:1095687/68‑1 (MQ=255)
                             cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCtt                                >  1:1261618/1‑68 (MQ=255)
                             cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCtt                                >  1:761694/1‑68 (MQ=255)
                             cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCtt                                <  1:142144/68‑1 (MQ=255)
                             cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCtt                                >  1:1425749/1‑68 (MQ=255)
                             cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCtt                                >  1:289809/1‑68 (MQ=255)
                             cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCtt                                <  1:2856883/68‑1 (MQ=255)
                             cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCtt                                >  1:232748/1‑68 (MQ=255)
                             cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCtt                                >  1:483711/1‑68 (MQ=255)
                             cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCtt                                >  1:2131274/1‑68 (MQ=255)
                             cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCtt                                <  1:1062208/68‑1 (MQ=255)
                             cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCtt                                >  1:1777054/1‑68 (MQ=255)
                             cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCtt                                >  1:2226541/1‑68 (MQ=255)
                             cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCtt                                >  1:1640788/1‑68 (MQ=255)
                             cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCtt                                >  1:2512498/1‑68 (MQ=255)
                             cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCtt                                <  1:2500486/68‑1 (MQ=255)
                             cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCtt                                <  1:2474378/68‑1 (MQ=255)
                             cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCtt                                <  1:2244462/68‑1 (MQ=255)
                             cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCtt                                >  1:1707576/1‑68 (MQ=255)
                             cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCt                                 >  1:796220/1‑67 (MQ=255)
                                   gCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGt                         >  1:3128137/1‑69 (MQ=255)
                                   gCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGt                         >  1:1608156/1‑69 (MQ=255)
                                   gCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGt                         >  1:2435094/1‑69 (MQ=255)
                                   gCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGt                         >  1:1689515/1‑69 (MQ=255)
                                       cATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGTcgcg                     <  1:2467606/69‑1 (MQ=255)
                                       cATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGTcgcg                     <  1:3147998/69‑1 (MQ=255)
                                       cATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGTcgcg                     <  1:2984613/69‑1 (MQ=255)
                                       cATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGTcgcg                     <  1:2818374/69‑1 (MQ=255)
                                       cATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGTcgcg                     <  1:270725/69‑1 (MQ=255)
                                        aTGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCTTACCACGTtgcg                     >  1:204386/1‑68 (MQ=255)
                                        aTGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCTTACCACGTtgcg                     >  1:1202671/1‑68 (MQ=255)
                                        aTGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCTTACCACGTtgcg                     >  1:1569497/1‑68 (MQ=255)
                                        aTGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCTTACCACGTtgcg                     >  1:1839316/1‑68 (MQ=255)
                                        aTGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCTTACCACGTtgcg                     >  1:496218/1‑68 (MQ=255)
                                        aTGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCTTACCACGTtgcg                     >  1:3068456/1‑68 (MQ=255)
                                        aTGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCTTACCACGTtgc                      >  1:611489/1‑67 (MQ=255)
                                        aTGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTCCAactact                                     >  1:857312/1‑52 (MQ=37)
                                             gAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGAt                >  1:713376/1‑68 (MQ=255)
                                             gAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCtt                                <  1:2251597/52‑1 (MQ=255)
                                                      aTTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCAcc      <  1:3248055/69‑1 (MQ=255)
                                                        tGCCCCGATCTTCTACACCGTTCCGCTGCCGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCACCGa    >  1:1713729/1‑69 (MQ=255)
                                                        tGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCACCGa    >  1:1854194/1‑69 (MQ=255)
                                                        tGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCACCGa    >  1:1614631/1‑69 (MQ=255)
                                                        tGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTAACCATGTCGCGCTGATCAAAGGCACCGa    >  1:1921150/1‑69 (MQ=255)
                                                         gCACCGATCTTCTACACCTTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCACCGAc   <  1:2844592/69‑1 (MQ=255)
                                                         gCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCACCGAc   >  1:1028603/1‑69 (MQ=255)
                                                         gCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCACCGAc   >  1:2122964/1‑69 (MQ=255)
                                                         gCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCACCGAc   <  1:2424053/69‑1 (MQ=255)
                                                           aCCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAgg          >  1:935837/1‑60 (MQ=255)
                                                           aCCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCACCGACg  >  1:1818720/1‑68 (MQ=255)
                                                           aCCGATCTTCTACACCGTTCCGCTGCAGCTGCTGCCTTACCATGTCGCGCTGATCAAAGGCACCGACg  >  1:3395163/1‑68 (MQ=255)
                                                              |                                                                
TTTGTAAGTAGCGATAACATGCACATCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCACCGACG  >  minE/2474385‑2474511

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: