| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,474,474 | G→A | 31.9% | L583L (CTG→CTA) | glmS → | L‑glutamine:D‑fructose‑6‑phosphate aminotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,474,474 | 0 | G | A | 31.9% | 115.7 / 64.7 | 128 | L583L (CTG→CTA) | glmS | L‑glutamine:D‑fructose‑6‑phosphate aminotransferase |
| Reads supporting (aligned to +/- strand): ref base G (54/33); new base A (29/12); total (83/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.28e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01 | |||||||||||
Only 100 of 142 total aligned reads displayed.
CACATCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCACCGACGTTGACCAGCCGCGTA > minE/2474406‑2474526 | cacaTCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAgctg > 1:58056/1‑69 (MQ=255)cacaTCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAgctg > 1:343929/1‑69 (MQ=255)cacaTCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAgctg > 1:1529782/1‑69 (MQ=255)cacaTCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAgctg > 1:1338498/1‑69 (MQ=255)cacaTCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAgctg > 1:1776186/1‑69 (MQ=255)cacaTCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAgctg > 1:2934127/1‑69 (MQ=255)cacaTCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAgctg > 1:1925997/1‑69 (MQ=255)cacaTCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAgctg > 1:925708/1‑69 (MQ=255)cacaTCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAgctg > 1:969507/1‑69 (MQ=255) tcatcGAGATGCCGCATGTGGAAGAGGTTATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTgg > 1:1768700/1‑69 (MQ=255) atcGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCt > 1:822839/1‑69 (MQ=255) atcGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCt > 1:1391662/1‑69 (MQ=255) atcGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCt > 1:2525098/1‑69 (MQ=255) atcGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCt > 1:576787/1‑69 (MQ=255) atcGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCt > 1:2040144/1‑69 (MQ=255) atcGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCt > 1:1549987/1‑69 (MQ=255) atcGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCt > 1:978065/1‑69 (MQ=255) cGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCTCTGCAGCTGCTGGCtt < 1:1233775/68‑1 (MQ=255) cGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCtt < 1:3265696/68‑1 (MQ=255) cGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCtt < 1:540319/68‑1 (MQ=255) cGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCtt > 1:2816408/1‑68 (MQ=255) cGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCtt < 1:681900/68‑1 (MQ=255) cGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCtt < 1:1012889/68‑1 (MQ=255) cGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCt > 1:3176485/1‑67 (MQ=255) cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCTACTACTGGCtt < 1:2023679/68‑1 (MQ=255) cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCtt > 1:2226541/1‑68 (MQ=255) cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCtt < 1:2248472/68‑1 (MQ=255) cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCtt > 1:2700521/1‑68 (MQ=255) cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCtt < 1:2856883/68‑1 (MQ=255) cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCtt < 1:2500486/68‑1 (MQ=255) cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCtt > 1:232748/1‑68 (MQ=255) cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCtt > 1:2512498/1‑68 (MQ=255) cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCtt < 1:2474378/68‑1 (MQ=255) cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCtt < 1:1095687/68‑1 (MQ=255) cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCtt > 1:761694/1‑68 (MQ=255) cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCtt > 1:1707576/1‑68 (MQ=255) cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCtt < 1:1692149/68‑1 (MQ=255) cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCtt < 1:142144/68‑1 (MQ=255) cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCtt > 1:1425749/1‑68 (MQ=255) cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCtt > 1:1640788/1‑68 (MQ=255) cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCtt > 1:483711/1‑68 (MQ=255) cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCtt > 1:1578485/1‑68 (MQ=255) gCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGt > 1:3015631/1‑69 (MQ=255) gCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGt > 1:2435094/1‑69 (MQ=255) gCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGt > 1:1689515/1‑69 (MQ=255) gCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGt > 1:3368971/1‑69 (MQ=255) gCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGt > 1:3128137/1‑69 (MQ=255) cATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGTcgcg < 1:270725/69‑1 (MQ=255) cATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGTcgcg < 1:534199/69‑1 (MQ=255) cATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGTcgcg < 1:2818374/69‑1 (MQ=255) cATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGTcgcg < 1:3177/69‑1 (MQ=255) cATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGTcgcg < 1:816678/69‑1 (MQ=255) cATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGTcgcg < 1:2467606/69‑1 (MQ=255) cATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGTcgcg < 1:1992231/69‑1 (MQ=255) aTGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCTTACCACGTtgcg > 1:833720/1‑68 (MQ=255) aTGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCTTACCACGTtgcg > 1:496218/1‑68 (MQ=255) aTGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCTTACCACGTtgcg > 1:1304298/1‑68 (MQ=255) aTGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCTTACCACGTtgcg > 1:1839316/1‑68 (MQ=255) aTGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCTTACCACGTtgcg > 1:3068456/1‑68 (MQ=255) aTGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCTTACCACGTtgcg > 1:1067231/1‑68 (MQ=255) aTGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCTTACCACGTtgc > 1:611489/1‑67 (MQ=255) gAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGAt > 1:713376/1‑68 (MQ=255) gAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCtt < 1:2251597/52‑1 (MQ=255) aTTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCAcc < 1:2948561/69‑1 (MQ=255) aTTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCAcc < 1:3248055/69‑1 (MQ=255) tGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCACCGa > 1:1434079/1‑69 (MQ=255) tGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCACCGa > 1:1854194/1‑69 (MQ=255) tGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCACCGa > 1:1614631/1‑69 (MQ=255) tGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCACCGa > 1:3142998/1‑69 (MQ=255) tGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTAACCATGTCGCGCTGATCAAAGGCACCGa > 1:1921150/1‑69 (MQ=255) gCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCACCGAc > 1:2122964/1‑69 (MQ=255) gCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCACCGAc > 1:1028603/1‑69 (MQ=255) gCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCACCGAc < 1:2424053/69‑1 (MQ=255) aCCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAgg > 1:935837/1‑60 (MQ=255) aCCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCACCGACg > 1:1818720/1‑68 (MQ=255) aCCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCACCGACg > 1:2845565/1‑68 (MQ=255) cttctACACCGTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCACCGACGTTGAcc > 1:2308908/1‑68 (MQ=255) ttctACACCGTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCACCGACGTTGACCAg > 1:2587824/1‑69 (MQ=255) ccGTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCACCGACGTTGACCAGCCGCGTa < 1:1140540/69‑1 (MQ=255) ccGTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCACCGACGTTGACCAGCCGCGTa < 1:1185404/69‑1 (MQ=255) ccGTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCACCGACGTTGACCAGCCGCGTa < 1:745457/69‑1 (MQ=255) ccGTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCACCGACGTTGACCAGCCGCGTa < 1:1137419/69‑1 (MQ=255) ccGTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCACCGACGTTGACCAGCCGCGTa < 1:1217289/69‑1 (MQ=255) ccGTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCACCGACGTTGACCAGCCGCGTa < 1:1015686/69‑1 (MQ=255) ccGTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCACCGACGTTGACCAGCCGCGTa < 1:1499070/69‑1 (MQ=255) ccGTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCACCGACGTTGACCAGCCGCGTa < 1:407242/69‑1 (MQ=255) ccGTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCACCGACGTTGACCAGCCGCGTa < 1:3414794/69‑1 (MQ=255) ccGTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCACCGACGTTGACCAGCCGCGTa < 1:3280147/69‑1 (MQ=255) ccGTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCACCGACGTTGACCAGCCGCGTa < 1:3257212/69‑1 (MQ=255) ccGTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCACCGACGTTGACCAGCCGCGTa < 1:3138811/69‑1 (MQ=255) ccGTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCACCGACGTTGACCAGCCGCGTa < 1:2091644/69‑1 (MQ=255) gTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCACCGACGtt > 1:1447620/1‑54 (MQ=255) gTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCACCGACGtt > 1:1588300/1‑54 (MQ=255) gTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCACCGACGtt > 1:1791268/1‑54 (MQ=255) gTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCACCGACGtt > 1:3365324/1‑54 (MQ=255) gTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCACCGACGtt > 1:3275949/1‑54 (MQ=255) gTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCACCGACGtt > 1:2919327/1‑54 (MQ=255) gTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCACCGACGtt > 1:2134798/1‑54 (MQ=255) gTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCACCGACGtt > 1:2251819/1‑54 (MQ=255) gTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCACCGACGt > 1:1816355/1‑53 (MQ=255) | CACATCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCACCGACGTTGACCAGCCGCGTA > minE/2474406‑2474526 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |