| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,475,894 | T→C | 38.6% | G342G (GGT→GGC) | pstS → | phosphate transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,475,894 | 0 | T | C | 38.6% | 17.5 / 26.6 | 44 | G342G (GGT→GGC) | pstS | phosphate transporter subunit |
| Reads supporting (aligned to +/- strand): ref base T (14/13); new base C (13/4); total (27/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.24e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01 | |||||||||||
TGTAGTTGAACAGGTTCGCGCTGCGTGGAAGACCAATATTAAAGACAGTAGCGGTAAGCCGCTGTACTAATAAAACTCCAGGCCGGGTACGGTGTTTTACGCCGCATCCGG > minE/2475840‑2475950 | cgtAGTTGAACAGGTTCGCGCTGCGTGGAAGACCAATATTAAAGACAGTAGCGGCAAGCCGCTGTaat > 1:1847705/2‑66 (MQ=255)cgtAGTTGAACAGGTTCGCGCTGCGTGGAAGACCAATATTAAAGACAGTAGCGGCAAGCCGCTGTACt > 1:1070836/2‑68 (MQ=255)cgtAGTTGAACAGGTTCGCGCTGCGTGGAAGACCAATATTAAAGACAGTAGCGGCAAGCCGCTGTACt > 1:1048603/2‑68 (MQ=255)cgtAGTTGAACAGGTTCGCGCTGCGTGGAAGACCAATATTAAAGACAGTAGCGGCAAGCCGCTGTACt > 1:903891/2‑68 (MQ=255)cgtAGTTGAACAGGTTCGCGCTGCGTGGAAGACCAATATTAAAGACAGTAGCGGCAAGCCGCTGTACt > 1:769023/2‑68 (MQ=255)cgtAGTTGAACAGGTTCGCGCTGCGTGGAAGACCAATATTAAAGACAGTAGCGGCAAGCCGCTGTACt > 1:497106/2‑68 (MQ=255)cgtAGTTGAACAGGTTCGCGCTGCGTGGAAGACCAATATTAAAGACAGTAGCGGCAAGCCGCTGTACt > 1:2846159/2‑68 (MQ=255)cgtAGTTGAACAGGTTCGCGCTGCGTGGAAGACCAATATTAAAGACAGTAGCGGCAAGCCGCTGTACt > 1:2657719/2‑68 (MQ=255)cgtAGTTGAACAGGTTCGCGCTGCGTGGAAGACCAATATTAAAGACAGTAGCGGCAAGCCGCTGTACt > 1:1645933/2‑68 (MQ=255)cgtAGTTGAACAGGTTCGCGCTGCGTGGAAGACCAATATTAAAGACAGTAGCGGCAAGCCGCTGTACt > 1:2640731/2‑68 (MQ=255)cgtAGTTGAACAGGTTCGCGCTGCGTGGAAGACCAATATTAAAGACAGTAGCGGCAAGCCGCTGTACt > 1:2576746/2‑68 (MQ=255)cgtAGTTGAACAGGTTCGCGCTGCGTGGAAGACCAATATTAAAGACAGTAGCGGCAAGCCGCTGTACt > 1:2443059/2‑68 (MQ=255)cgtAGTTGAACAGGTTCGCGCTGCGTGGAAGACCAATATTAAAGACAGTAGCGGCAAGCCGCTGTACt > 1:2327961/2‑68 (MQ=255) cAGGTTCGCGCTGCGTGGAAGACCAATATTAAAGACAGTAGCGGCAAGCCGCTGTACTAATAAAACTcc < 1:982252/69‑1 (MQ=255) cAGGTTCGCGCTGCGTGGAAGACCAATATTAAAGACAGTAGCGGCAAGCCGCTGTACTAATAAAACTcc < 1:2411608/69‑1 (MQ=255) cAGGTTCGCGCTGCGTGGAAGACCAATATTAAAGACAGTAGCGGCAAGCCGCTGTACTAATAAAACTcc < 1:2091154/69‑1 (MQ=255) aGGTTCGCGCTGCGTGGAAGACCAATATTAAAGACAGTAGCGGCAAGCCGCTGTACTAATAAAACTcc < 1:570448/68‑1 (MQ=255) cgcgCTGCGTGGAAGACCAATATTAAAGACAGTAGCGGTAAGCCGCTGTACTAATaaaa < 1:2098308/59‑1 (MQ=255) cgcgCTGCGTGGAAGACCAATATTAAAGACAGTAGCGGTAAGCCGCTGTACTAATaaaa < 1:869780/59‑1 (MQ=255) cgcgCTGCGTGGAAGACCAATATTAAAGACAGTAGCGGTAAGCCGCTGTACTAATaaaa < 1:2997036/59‑1 (MQ=255) cgcgCTGCGTGGAAGACCAATATTAAAGACAGTAGCGGTAAGCCGCTGTACTAATaaaa < 1:297575/59‑1 (MQ=255) cgcgCTGCGTGGAAGACCAATATTAAAGACAGTAGCGGTAAGCCGCTGTACTAATaaaa < 1:2078442/59‑1 (MQ=255) cgcgCTGCGTGGAAGACCAATATTAAAGACAGTAGCGGTAAGCCGCTGTACTAATaaaa < 1:2346959/59‑1 (MQ=255) gAAGACCAATATTAAAGACAGTAGCGGTAAGCCGCTGTACTAATAAAACTCCAGGCCGGGTACGgtgt > 1:946749/1‑68 (MQ=255) ccAATATTAAAGACAGTAGCGGTAAGCCGCTGTACTAATAAAACt > 1:2039805/1‑45 (MQ=255) ccAATATTAAAGACAGTAGCGGTAAGCCGCTGTACTAATAAAACTCCAGGCCGGGTACGGTGTTTTAcg > 1:185087/1‑69 (MQ=255) ccAATATTAAAGACAGTAGCGGTAAGCCGCTGTACTAATAAAACTCCAGGCCGGGTACGGTGTTTTAcg > 1:1473846/1‑69 (MQ=255) ccAATATTAAAGACAGTAGCGGTAAGCCGCTGTACTAATAAAACTCCAGGCCGGGTACGGTGTTTTAcg > 1:1353648/1‑69 (MQ=255) ccAATATTAAAGACAGTAGCGGTAAGCCGCTGTACTAATAAAACTCCAGGCCGGGTACGGTGTTTTAcg > 1:727033/1‑69 (MQ=255) ccAATATTAAAGACAGTAGCGGTAAGCCGCTGTACTAATAAAACTCCAGGCCGGGTACGGTGTTTTAc > 1:2127817/1‑68 (MQ=255) atTAAAGACAGTAGCGGTAAGCCGCTGTACTAATAAAACTCCAGGCCGGGTACGGTGTTTTACGCCGCa < 1:1575876/69‑1 (MQ=255) atTAAAGACAGTAGCGGTAAGCCGCTGTACTAATAAAACTCCAGGCCGGGTACGGTGTTTTACGCCGCa < 1:520589/69‑1 (MQ=255) ttAAAGACAGTAGCGGTAAGCCGCTGTACtaataa > 1:1276337/1‑35 (MQ=255) ttAAAGACAGTAGCGGTAAGCCGCTGTACtaataa > 1:1429555/1‑35 (MQ=255) ttAAAGACAGTAGCGGTAAGCCGCTGTACTAATAAAACTCCAGGCCGGGTACGGTGTTTTATGCCGCAt > 1:859472/1‑69 (MQ=255) ttAAAGACAGTAGCGGTAAGCCGCTGTACTAATAAAACTCCAGGCCGGGTACGGTGTTTTACGCCGCAt > 1:1267950/1‑69 (MQ=255) ttAAAGACAGTAGCGGTAAGCCGCTGTACTAATAAAACTCCAGGCCGGGTACGGTGTTTTACGCCGCAt > 1:795569/1‑69 (MQ=255) ttAAAGACAGTAGCGGTAAGCCGCTGTACTAATAAAACTCCAGGCCGGGTACGGTGTTTTACGCCGCAt > 1:2634133/1‑69 (MQ=255) gACAGTAGCGGTAAGCCGCTGTACTAATAAAACTCCAGGCCGGGTACGGTGTTTTACGCCGCATCCgg < 1:2379422/68‑1 (MQ=255) gACAGTAGCGGTAAGCCGCTGTACTAATAAAACTCCAGGCCGGGTACGGTGTTTTACGCCGCATCCgg < 1:846885/68‑1 (MQ=255) gACAGTAGCGGTAAGCCGCTGTACTAATAAAACTCCAGGCCGGGTACGGTGTTTTACGCCGCATCCgg < 1:24330/68‑1 (MQ=255) gACAGTAGCGGTAAGCCGCTGTACTAATAAAACTCCAGGCCGGGTACGGTGTTTTACGCCGCATCCgg < 1:2701050/68‑1 (MQ=255) gACAGTAGCGGTAAGCCGCTGTACTAATAAAACTCCAGGCCGGGTACGGTGTTTTACGCCGCATCCgg < 1:2546514/68‑1 (MQ=255) aGCGGTAAGCCGCTGTACTAATAAAACTCCAGGCCGGGTACGGTGTTTTACGCCGCATCCgg > 1:521334/1‑62 (MQ=255) tAAGCCGCTGTACTAATAAAACTCCAGGCCGGGTACGGTGTTTTACGCCGCATCCgg < 1:2508910/57‑1 (MQ=255) | TGTAGTTGAACAGGTTCGCGCTGCGTGGAAGACCAATATTAAAGACAGTAGCGGTAAGCCGCTGTACTAATAAAACTCCAGGCCGGGTACGGTGTTTTACGCCGCATCCGG > minE/2475840‑2475950 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |