| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,476,553 | G→A | 32.9% | A186A (GCG→GCA) | pstC → | phosphate transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,476,553 | 0 | G | A | 32.9% | 81.5 / 50.3 | 97 | A186A (GCG→GCA) | pstC | phosphate transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (42/23); new base A (19/13); total (61/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.59e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01 | |||||||||||
Only 100 of 106 total aligned reads displayed.
ATTTGGTATCGGTATCCTCGCGGCAGGCGTGATCCTCGCCATCATGATTATTCCGTACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAAGAGTCGGCCTACGGTATTGGC > minE/2476490‑2476619 | aTTTGGTATCGGTATCCTCGCGGCAGGCGTGATCCTCGCCATCATGATTATTCCGTACATTGCGGCGGt < 1:922566/69‑1 (MQ=255)aTTTGGTATCGGTATCCTCGCGGCAGGCGTGATCCTCGCCATCATGATTATTCCGTACATTGCGGCGGt < 1:470586/69‑1 (MQ=255) tttGGTATCGGTATCCTCGCGGCAGGCGTGATCCTCGCCATCATGATTATTCCGTACATTGCAGCGGTa < 1:3144114/69‑1 (MQ=255) tttGGTATCGGTATCCTCGCGGCAGGCGTGATCCTCGCCATCATGATTATTCCGTACATTGCAGCGGTa < 1:2552796/69‑1 (MQ=255) tttGGTATCGGTATCCTCGCGGCAGGCGTGATCCTCGCCATCATGATTATTCCGTACATTGCAGCGGTa < 1:2637149/69‑1 (MQ=255) tttGGTATCGGTATCCTCGCGGCAGGCGTGATCCTCGCCATCATGATTATTCCGTACATTGCAGCGGTa < 1:545312/69‑1 (MQ=255) tttGGTATCGGTATCCTCGCGGCAGGCGTGATCCTCGCCATCATGATTATTCCGTACATTGCAGCGGTa < 1:2336409/69‑1 (MQ=255) tttGGTATCGGTATCCTCGCGGCAGGCGTGATCCTCGCCATCATGATTATTCCGTACATTGCAGCGGTa < 1:2298143/69‑1 (MQ=255) tttGGTATCGGTATCCTCGCGGCAGGCGTGATCCTCGCCATCATGATTATTCCGTACATTGCAGCGGTa < 1:2765952/69‑1 (MQ=255) tttGGTATCGGTATCCTCGCGGCAGGCGTGATCCTCGCCATCATGATTATTCCGTACATTGCAGCGGTa < 1:2848059/69‑1 (MQ=255) tttGGTATCGGTATCCTCGCGGCAGGCGTGATCCTCGCCATCATGATTATTCCGTACATTGCAGCGGTa < 1:1905293/69‑1 (MQ=255) tttGGTATCGGTATCCTCGCGGCAGGCGTGATCCTCGCCATCATGATTATTCCGTACATTGCAGCGGTa < 1:2943656/69‑1 (MQ=255) tttGGTATCGGTATCCTCGCGGCAGGCGTGATCCTCGCCATCATGATTATTCCGTACATTGCAGCGGTa < 1:423067/69‑1 (MQ=255) tttGGTATCGGTATCCTCGCGGCAGGCGTGATCCTCGCCATCATGATTATTCCGTACATTGCAGCGGTa < 1:192717/69‑1 (MQ=255) tttGGTATCGGTATCCTCGCGGCAGGCGTGATACTCGCCATCATGATTATTCCGTACATTGCAGCGGTa < 1:3023227/69‑1 (MQ=255) ggtatcCTCGCGGCAGGCGTGATCCTCGCCATCATGATTATTCCGTACATTGCGGCGGt < 1:2447242/59‑1 (MQ=255) ggtatcCTCGCGGCAGGCGTGATCCTCGCCATCATGATTATTCCGTACATTGCGGCGGt < 1:46152/59‑1 (MQ=255) ggtatcCTCGCGGCAGGCGTGATCCTCGCCATCATGATTATTCCGTACATTGCGGCGGt < 1:1481239/59‑1 (MQ=255) gtatcCTCGCGGCAGGCGTGATCCTCGCCATCATGATTATTCCGTACATTGCGGCGGt < 1:810893/58‑1 (MQ=255) tcCTCGCGTCAGGCGTGATCCTCGCCATCATGATTATTCCGTACATTGCGGCGGt < 1:1807071/55‑1 (MQ=255) tcCTCGCGGCAGGCGTGATCCTCGCCATCATGATTATTCCGTACATTGCGGCGGt < 1:475424/55‑1 (MQ=255) cTCGCGTCAGGCGTGATCCTCGCCATCATGATTATTCTGTACATAGCGGCGGTAATGCGTGATGTGtt > 1:3943/1‑68 (MQ=255) cTCGCGGCAGGCGTGATCCTCGCCATCATGATTATTCCGTACATTGCGGCGGTAATGCGTGATGTGtt > 1:2966117/1‑68 (MQ=255) cTCGCGGCAGGCGTGATCCTCGCCATCATGATTATTCCGTACATTGCGGCGGTAATGCGTGATGTGtt > 1:1119430/1‑68 (MQ=255) cTCGCGGCAGGCGTGATCCTCGCCATCATGATTATTCCGTACATTGCGGCGGTAATGCGTGATGTGtt > 1:1798921/1‑68 (MQ=255) cTCGCGGCAGGCGTGATCCTCGCCATCATGATTATTCCGTACATTGCGGCGGTAATGCGTGATGTGtt > 1:1782432/1‑68 (MQ=255) gTGATCCTCGCCATCATGATTATTCCGTACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCg > 1:71009/1‑69 (MQ=255) gTGATCCTCGCCATCATGATTATTCCGTACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCg > 1:151939/1‑69 (MQ=255) gTGATCCTCGCCATCATGATTATTCCGTACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCg > 1:308041/1‑69 (MQ=255) gTGATCCTCGCCATCATGATTATTCCGTACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCg > 1:1465283/1‑69 (MQ=255) gTGATCCTCGCCATCATGATTATTCCGTACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCg > 1:2186693/1‑69 (MQ=255) gATCCTCGCCATCATGATTATTGCGTACATTGCGGCGGt < 1:2743586/39‑1 (MQ=255) aTCCTCGCCATCATGATTATTCCGTACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGtg > 1:3402552/1‑69 (MQ=255) tCCTCGCCATCATGATTATTCCGTACATTGCAGCGGTAAt > 1:857674/1‑40 (MQ=255) tCCTCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGa > 1:2569033/1‑55 (MQ=255) tCCTCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGa > 1:83774/1‑55 (MQ=255) tCCTCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGa > 1:686650/1‑55 (MQ=255) tCCTCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGa > 1:765306/1‑55 (MQ=255) tCCTCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGa > 1:529986/1‑55 (MQ=255) tCCTCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGa > 1:775535/1‑55 (MQ=255) tCCTCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGa > 1:3003768/1‑55 (MQ=255) tCCTCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGa > 1:308276/1‑55 (MQ=255) tCCTCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGa > 1:984246/1‑55 (MQ=255) tCCTCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGa > 1:1851411/1‑55 (MQ=255) tCCTCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGa > 1:2469420/1‑55 (MQ=255) tCCTCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGa > 1:2373672/1‑55 (MQ=255) tCCTCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGa > 1:2350430/1‑55 (MQ=255) tCCTCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGa > 1:1810602/1‑55 (MQ=255) tCCTCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGa > 1:981655/1‑55 (MQ=255) tCCTCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGa > 1:1254171/1‑55 (MQ=255) tCCTCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGa > 1:2161345/1‑55 (MQ=255) atGATTATTCCGTACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGtgat < 1:1419628/59‑1 (MQ=255) atGATTATTCCGTACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGtgat < 1:1523727/59‑1 (MQ=255) atGATTATTCCGTACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGtgat < 1:2889615/59‑1 (MQ=255) atGATTATTCCGTACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGtgat < 1:85377/59‑1 (MQ=255) atGATTATTCCGTACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGtgat < 1:2416764/59‑1 (MQ=255) atGATTATTCCGTACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAagag > 1:767642/1‑69 (MQ=255) atGATTATTCCGTACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAagag > 1:630688/1‑69 (MQ=255) atGATTATTCCGTACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAagag > 1:1486008/1‑69 (MQ=255) atGATTATTCCGTACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAagag > 1:951008/1‑69 (MQ=255) atGATTATTCCGTACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAagag > 1:1590535/1‑69 (MQ=255) atGATTATTCCGTACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAagag > 1:3414424/1‑69 (MQ=255) atGATTATTCCGTACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAagag > 1:807133/1‑69 (MQ=255) atGATTATTCCGTACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAagag > 1:2378515/1‑69 (MQ=255) atGATTATTCCGTACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAagag > 1:27139/1‑69 (MQ=255) atGATTATTCCGTACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAagag > 1:1847812/1‑69 (MQ=255) atGATTATTCCGTACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAagag > 1:2070802/1‑69 (MQ=255) atGATTATTCCGTACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAagag > 1:2956410/1‑69 (MQ=255) atGATTATTCCGTACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAagag > 1:267696/1‑69 (MQ=255) atGATTATTCCGTACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAagag > 1:1851480/1‑69 (MQ=255) atGATTATTCCGTACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAaga > 1:2165162/1‑68 (MQ=255) atGATTATTCCGTACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACACCGGTGATGATGAAagag > 1:1004133/1‑69 (MQ=255) attattCCGTACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAAGAGTCg > 1:124747/1‑69 (MQ=255) attCCGTACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAAGAGTCGGcc < 1:890956/69‑1 (MQ=255) attCCGTACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAAGAGTCGGcc > 1:211023/1‑69 (MQ=255) attCCGTACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAAGAGTCGGcc > 1:484876/1‑69 (MQ=255) attCCGTACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAAGAGTCGGcc > 1:526798/1‑69 (MQ=255) attCCGTACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAAGAGTCGGcc < 1:1102769/69‑1 (MQ=255) tACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAAGAGTCGGCCTACgg < 1:737028/68‑1 (MQ=255) tACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAAGAGTCGGCCTACgg < 1:1672028/68‑1 (MQ=255) tACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAAGAGTCGGCCTACgg < 1:76610/68‑1 (MQ=255) cATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGtgatgatga > 1:2257899/1‑50 (MQ=255) cATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGtgatgatga > 1:57974/1‑50 (MQ=255) cATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGtgatgatga > 1:1566672/1‑50 (MQ=255) cATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGtgatgatga > 1:1619349/1‑50 (MQ=255) cATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGtgatgatga > 1:3352345/1‑50 (MQ=255) cATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGtgatgatga > 1:3018630/1‑50 (MQ=255) cATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGtgatgatga > 1:1823958/1‑50 (MQ=255) cATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAAGAGTCGGCCTACGGTAt > 1:2708266/1‑69 (MQ=255) cATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAAGAGTCGGCCTACGGTAt > 1:1232612/1‑69 (MQ=255) cATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAAGAGTCGGCCTACGGTAt > 1:1066113/1‑69 (MQ=255) gcggcgGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAAGAGTCGGCCTACGGTATTgg > 1:3038200/1‑68 (MQ=255) gcggcgGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAAGAGTCGGCCTACGGTATTgg > 1:2892796/1‑68 (MQ=255) gcggcgGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAAGAGTCGGCCTACGGTATTgg > 1:2541834/1‑68 (MQ=255) gcggcgGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAAGAGTCGGCCTACGGTATTGGc > 1:359835/1‑69 (MQ=255) gcggcgGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAAGAGTCGGCCTACGGTATTGGc > 1:3316425/1‑69 (MQ=255) gcggcgGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAAGAGTCGGCCTACGGTATTGGc > 1:2664529/1‑69 (MQ=255) gcggcgGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAAGAGTCGGCCTACGGTATTGGc > 1:1983516/1‑69 (MQ=255) gcggcgGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAAGAGTCGGCCTACGGTATTGGc > 1:1046891/1‑69 (MQ=255) gcggcgGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAAGAGTCGGCCTACGGTATTGGc > 1:1008763/1‑69 (MQ=255) | ATTTGGTATCGGTATCCTCGCGGCAGGCGTGATCCTCGCCATCATGATTATTCCGTACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAAGAGTCGGCCTACGGTATTGGC > minE/2476490‑2476619 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |