| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,478,336 | T→C | 31.5% | F103F (TTT→TTC) | pstB → | phosphate transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,478,336 | 0 | T | C | 31.5% | 97.1 / 59.0 | 114 | F103F (TTT→TTC) | pstB | phosphate transporter subunit |
| Reads supporting (aligned to +/- strand): ref base T (30/48); new base C (21/15); total (51/63) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.77e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 119 total aligned reads displayed.
AACTCTCAGGATATCGCACTGCTGCGTGCGAAAGTGGGCATGGTGTTCCAGAAACCGACGCCGTTTCCGATGTCCATCTACGACAACATCGCTTTTGGCGTTCGTCTGTTTGAGAAGCTCTCCCGTGCCGACA > minE/2478271‑2478403 | aaCTCTCAGGATATCGCACTGCTGCGTGCGAAAGTGGGCATGGTGTTCCAGAAACCGACGCCGTTTCCg > 1:3297931/1‑69 (MQ=255)aaCTCTCAGGATATCGCACTGCTGCGTGCGAAAGTGGGCATGGTGTTCCAGAAACCGACGCCGTTTCCg > 1:291837/1‑69 (MQ=255)aaCTCTCAGGATATCGCACTGCTGCGTGCGAAAGTGGGCATGGTGTTCCAGAAACCGACGCCGTTTCCg > 1:3138597/1‑69 (MQ=255)aaCTCTCAGGATATCGCACTGCTGCGTGCGAAAGTGGGCATGGTGTTCCAGAAACCGACGCCGTTTCCg > 1:500033/1‑69 (MQ=255) aGGATATCGCACTGCTGCGTGCGAAAGTGGGCATGGTGTTCCAGAAACCGACGCCGTTTCCGATGTCCa > 1:1075137/1‑69 (MQ=255) gATATCGCACTGCTGCGTGCGAAAGTGGGCATGGTGTTCCAGAAACCGACGCCGTTTCCGATGTCCa < 1:1911965/67‑1 (MQ=255) gATATCGCACTGCTGCGTGCGAAAGTGGGCATGGTGTTCCAGAAACCGACGCCGTTTCCGATGTCCa < 1:3354550/67‑1 (MQ=255) gATATCGCACTGCTGCGTGCGAAAGTGGGCATGGTGTTCCAGAAACCGACGCCGTTTCCGATGTCCATc < 1:2794215/69‑1 (MQ=255) atatCGCACTGCTGCGTGCGAAAGTGGGCATGGTGTTCCAGAAACCGACGCCGTTTCCGATGTCCa < 1:410131/66‑1 (MQ=255) atatCGCACTGCTGCGTGCGAAAGTGGGCATGGTGTTCCAGAAACCGACGCCGTTTCCGATGTCCATc < 1:2380047/68‑1 (MQ=255) tatCGCACTGCTTCGTGCGAAAGTGGGCATGGTGTTCCAGAAACCGACGCCGTTTCCGATGTcc < 1:2701860/64‑1 (MQ=255) tCGCACTTCTGCGTGCGAAAGTGGGCATGGTGTTCCAGAAACCGACGCCGTTTCCGATGTCCATCTACg < 1:1435715/69‑1 (MQ=255) tCGCACTGCTGCGTGCGAAAGTGGGCATGGTGTTCCAGAAACCGACGCCGTTTCCGATGTCCATCTAc > 1:2158630/1‑68 (MQ=255) tCGCACTGCTGCGTGCGAAAGTGGGCATGGTGTTCCAGAAACCGACGCCGTTTCCGATGTCCATCTAc > 1:3287682/1‑68 (MQ=255) tCGCACTGCTGCGTGCGAAAGTGGGCATGGTGTTCCAGAAACCGACGCCGTTTCCGATGTCCATCTAc > 1:2514945/1‑68 (MQ=255) tCGCACTGCTGCGTGCGAAAGTGGGCATGGTGTTCCAGAAACCGACGCCGTTTCCGATGTCCATCTACg < 1:112344/69‑1 (MQ=255) tCGCACTGCTGCGTGCGAAAGTGGGCATGGTGTTCCAGAAACCGACGCCGTTTCCGATGTCCATCTACg < 1:321303/69‑1 (MQ=255) tCGCACTGCTGCGTGCGAAAGTGGGCATGGTGTTCCAGAAACCGACGCCGTTTCCGATGTCCATCTACg < 1:1538540/69‑1 (MQ=255) tCGCACTGCTGCGTGCGAAAGTGGGCATGGTGTTCCAGAAACCGACGCCGTTTCCGATGTCCATCTACg < 1:1607191/69‑1 (MQ=255) tCGCACTGCTGCGTGCGAAAGTGGGCATGGTGTTCCAGAAACCGACGCCGTTTCCGATGTCCATCTACg < 1:243806/69‑1 (MQ=255) tCGCACTGCTGCGTGCGAAAGTGGGCATGGTGTTCCAGAAACCGACGCCGTTTCCGATGTCCATCTACg < 1:247182/69‑1 (MQ=255) cGCACTGCTGCGTGCGAAAGTGGGCATGGTGTTCCAGAAACCGACGCCGTTTCCGATGTCCATCTACg < 1:2799638/68‑1 (MQ=255) cGCACTGCTGCGTGCGAAAGTGGGCATGGTGTTCCAGAAACCGACGCCGTTTCCGATGTCCATCTACg < 1:325345/68‑1 (MQ=255) gctgcGTGCGAAAGTGGGCATGGTGTTCCAGAAACCGACGCCGTTTCCGATGTCCATCTACGacaaca > 1:484944/1‑68 (MQ=255) gctgcGTGCGAAAGTGGGCATGGTGTTCCAGAAACCGACGCCGTTTCCGATGTCCATCTACGACAACAt > 1:495060/1‑69 (MQ=255) gctgcGTGCGAAAGTGGGCATGGTGTTCCAGAAACCGACGCCGTTTCCGATGTCCATCTACGACAACAt > 1:1251749/1‑69 (MQ=255) gctgcGTGCGAAAGTGGGCATGGTGTTCCAGAAACCGACGCCGTTTCCGATGTCCATCTACGACAACAt > 1:3242595/1‑69 (MQ=255) gcgtgcgAAAGTGGGCATGGTGTTCCAGAAACCGACGCCGTTCCCAATGTCCATCTACGACAACATCGc > 1:2557797/1‑69 (MQ=255) gcgtgcgAAAGTGGGCATGGTGTTCCAGAAACCGACGCCGTTCCCAATGTCCATCTACGACAACATCGc > 1:2601063/1‑69 (MQ=255) gcgtgcgAAAGTGGGCATGGTGTTCCAGAAACCGACGCCGTTCCCAATGTCCATCTACGACAACATCGc > 1:2740156/1‑69 (MQ=255) gcgtgcgAAAGTGGGCATGGTGTTCCAGAAACCGACGCCGTTCCCAATGTCCATCTACGACAACATCGc > 1:2431244/1‑69 (MQ=255) gcgtgcgAAAGTGGGCATGGTGTTCCAGAAACCGACGCCGTTCCCAATGTCCATCTACGACAACATCGc > 1:2778535/1‑69 (MQ=255) gcgtgcgAAAGTGGGCATGGTGTTCCAGAAACCGACGCCGTTCCCAATGTCCATCTACGACAACATCGc > 1:3041541/1‑69 (MQ=255) gcgtgcgAAAGTGGGCATGGTGTTCCAGAAACCGACGCCGTTCCCAATGTCCATCTACGACAACATCGc > 1:2260807/1‑69 (MQ=255) gcgtgcgAAAGTGGGCATGGTGTTCCAGAAACCGACGCCGTTCCCAATGTCCATCTACGACAACATCGc > 1:733348/1‑69 (MQ=255) gcgtgcgAAAGTGGGCATGGTGTTCCAGAAACCGACGCCGTTCCCAATGTCCATCTACGACAACATCGc > 1:937963/1‑69 (MQ=255) gcgtgcgAAAGTGGGCATGGTGTTCCAGAAACCGACGCCGTTCCCAATGTCCATCTACGACAACATCGc > 1:1050666/1‑69 (MQ=255) gcgtgcgAAAGTGGGCATGGTGTTCCAGAAACCGACGCCGTTCCCAATGTCCATCTACGACAACATCGc > 1:1067568/1‑69 (MQ=255) gcgtgcgAAAGTGGGCATGGTGTTCCAGAAACCGACGCCGTTCCCAATGTCCATCTACGACAACATCGc > 1:876702/1‑69 (MQ=255) gcgtgcgAAAGTGGGCATGGTGTTCCAGAAACCGACGCCGTTCCCAATGTCCATCTACGACAACATCGc > 1:114828/1‑69 (MQ=255) gcgtgcgAAAGTGGGCATGGTGTTCCAGAAACCGACGCCGTTCCCAATGTCCATCTACGACAACATCGc > 1:1472447/1‑69 (MQ=255) gcgtgcgAAAGTGGGCATGGTGTTCCAGAAACCGACGCCGTTCCCAATGTCCATCTACGACAACATCGc > 1:2234075/1‑69 (MQ=255) gcgtgcgAAAGTGGGCATGGTGTTCCAGAAACCGACGCCGTTCCCAATGTCCATCTACGACAACATCGc > 1:1856444/1‑69 (MQ=255) gcgtgcgAAAGTGGGCATGGTGTTCCAGAAACCGACGCCGTTCCCAATGTCCATCTACGACAACATCGc > 1:2019908/1‑69 (MQ=255) gtgtTCCAGAAACCGACGCCGTTTCCGATGTCCATCTACGACATCATCGCTTTTGGCGTTCGTCTGt > 1:1229997/1‑67 (MQ=255) gtgtTCCAGAAACCGACGCCGTTTCCGATGTCCATCTACGACAACATCGCTTTTGGCGTTCGTCTg > 1:1419036/1‑66 (MQ=255) gtgtTCCAGAAACCGACGCCGTTTCCGATGTCCATCTACGACAACATCGCTTTTGGCGTTCGTCTGttt < 1:2474945/69‑1 (MQ=255) gtgtTCCAGAAACCGACGCCGTTTCCGATGTCCATCTACGACAACATCGCTTTTGGCGTTCGTCTGttt < 1:897785/69‑1 (MQ=255) gtgtTCCAGAAACCGACGCCGTTTCCGATGTCCATCTACGACAACATCGCTTTTGGCGTTCGTCTGttt < 1:735968/69‑1 (MQ=255) gtgtTCCAGAAACCGACGCCGTTTCCGATGTCCATCTACGACAACATCGCTTTTGGCGTTCGTCTGttt < 1:581909/69‑1 (MQ=255) gtgtTCCAGAAACCGACGCCGTTTCCGATGTCCATCTACGACAACATCGCTTTTGGCGTTCGTCTGttt < 1:3396828/69‑1 (MQ=255) gtgtTCCAGAAACCGACGCCGTTTCCGATGTCCATCTACGACAACATCGCTTTTGGCGTTCGTCTGttt < 1:2524008/69‑1 (MQ=255) gtgtTCCAGAAACCGACGCCGTTTCCGATGTCCATCTACGACAACATCGCTTTTGGCGTTCGTCTGt > 1:2626364/1‑67 (MQ=255) gtgtTCCAGAAACCGACGCCGTTTCCGATGTCCATCTACGACAACATCGCTTTTGGCGTTCGTCTGt > 1:3246601/1‑67 (MQ=255) gtgtTCCAGAAACCGACGCCGTTTCCGATGTCCATCTACGACAACATCGCTTTTGGCGTTCGTCTGt > 1:2402123/1‑67 (MQ=255) gtTCCAGAAACCGACGCCGTTTCCGATGTCCATCTACGACAACATCGCTTTTGGCGTTCGTCt < 1:3036037/63‑1 (MQ=255) gtTCCAGAAACCGACGCCGTTTCCGATGTCCATCTACGACAACATCGCTTTTGGCGTTCGTCt < 1:2028598/63‑1 (MQ=255) gtTCCAGAAACCGACGCCGTTTCCGATGTCCATCTACGACAACATCGCTTTTGGCGTTCGTCt < 1:602491/63‑1 (MQ=255) gtTCCAGAAACCGACGCCGTTTCCGATGTCCATCTACGACAACATCGCTTTTGGCGTTCGTCt < 1:3163640/63‑1 (MQ=255) gtTCCAGAAACCGACGCCGTTTCCGATGTCCATCTACGACAACATCGCTTTTGGCGTTCGTCt < 1:1743669/63‑1 (MQ=255) gtTCCAGAAACCGACGCCGTTTCCGATGTCCATCTACGACAACATCGCTTTTGGCGTTCGTCt < 1:1392292/63‑1 (MQ=255) gtTCCAGAAACCGACGCCGTTTCCGATGTCCATCTACGACAACATCGCTTTTGGCGTTCGTCt < 1:1834296/63‑1 (MQ=255) gtTCCAGAAACCGACGCCGTTTCCGATGTCCATCTACGACAACATCGCTTTTGGCGTTCGTCt < 1:2010175/63‑1 (MQ=255) gtTCCAGAAACCGACGCCGTTTCCGATGTCCATCTACGACAACATCGCTTTTGGCGTTCGTCt < 1:1989070/63‑1 (MQ=255) gtTCCAGAAACCGACGCCGTTTCCGATGTCCATCTACGACAACATCGCTTTTGGCGTTCGTCt < 1:1649314/63‑1 (MQ=255) ccAGAAACCGACGCCGTTTCCGATGTCCATCTACGACAACATCGCTTTTGGCGTTCGTCTGTTTGAGaa > 1:3014335/1‑69 (MQ=255) ccAGAAACCGACGCCGTTTCCGATGTCCATCTACGACAACATCGCTTTTGGCGTTCGTCTGTTTGAGaa > 1:3312004/1‑69 (MQ=255) ccAGAAACCGACGCCGTTTCCGATGTCCATCTACGACAACATCGCTTTTGGCGTTCGTCTGTTTGAGaa > 1:745476/1‑69 (MQ=255) ccAGAAACCGACGCCGTTTCCGATGTCCATCTACGACAACATCGCTTTTGGCGTTCGTCTGTTTGAGaa > 1:2719627/1‑69 (MQ=255) ccAGAAACCGACGCCGTTTCCGATGTCCATCTACGACAACATCGCTTTTGGCGTTCGTCTGTTTGAGaa > 1:3297080/1‑69 (MQ=255) ccAGAAACCGACGCCGTTTCCGATGTCCATCTACGACAACATCGCTTTTGGCGTTCGTCTGTTTGAGaa > 1:2258316/1‑69 (MQ=255) aCCGACGCCGTTTCCGATGTCCATCTACGACAACATCGCTTTTGGCGTTCGTCTGTTTGAGAAGCtctc > 1:1018911/1‑69 (MQ=255) aCCGACGCCGTTTCCGATGTCCATCTACGACAACATCGCTTTTGGCGTTCGTCTGTTTGAGAAGCtctc > 1:108628/1‑69 (MQ=255) gACGCCGTTCCCAATGTCCATCTACGACAACATCGCTTTTGGCGTGCGTCTGTTTGAGAAGCTCTCCCg < 1:966883/69‑1 (MQ=255) gACGCCGTTCCCAATGTCCATCTACGACAACATCGCTTTTGGCGTGCGTCTGTTTGAGAAGCTCTCCCg < 1:759340/69‑1 (MQ=255) gACGCCGTTCCCAATGTCCATCTACGACAACATCGCTTTTGGCGTGCGTCTGTTTGAGAAGCTCTCCCg < 1:1128131/69‑1 (MQ=255) gACGCCGTTCCCAATGTCCATCTACGACAACATCGCTTTTGGCGTGCGTCTGTTTGAGAAGCTCTCCCg < 1:1519146/69‑1 (MQ=255) gACGCCGTTCCCAATGTCCATCTACGACAACATCGCTTTTGGCGTGCGTCTGTTTGAGAAGCTCTCCCg < 1:1018086/69‑1 (MQ=255) gACGCCGTTCCCAATGTCCATCTACGACAACATCGCTTTTGGCGTGCGTCTGTTTGAGAAGCTCTCCCg < 1:646796/69‑1 (MQ=255) gACGCCGTTCCCAATGTCCATCTACGACAACATCGCTTTTGGCGTGCGTCTGTTTGAGAAGCTCTCCCg < 1:1853013/69‑1 (MQ=255) gACGCCGTTCCCAATGTCCATCTACGACAACATCGCTTTTGGCGTGCGTCTGTTTGAGAAGCTCTCCCg < 1:2061314/69‑1 (MQ=255) gACGCCGTTCCCAATGTCCATCTACGACAACATCGCTTTTGGCGTGCGTCTGTTTGAGAAGCTCTCCCg < 1:959539/69‑1 (MQ=255) gACGCCGTTCCCAATGTCCATCTACGACAACATCGCTTTTGGCGTGCGTCTGTTTGAGAAGCTCTCCCg < 1:443801/69‑1 (MQ=255) gACGCCGTTCCCAATGTCCATCTACGACAACATCGCTTTTGGCGTGCGTCTGTTTGAGAAGCTCTCCCg < 1:2435200/69‑1 (MQ=255) gACGCCGTTCCCAATGTCCATCTACGACAACATCGCTTTTGGCGTGCGTCTGTTTGAGAAGCTCTCCCg < 1:2542581/69‑1 (MQ=255) gACGCCGTTCCCAATGTCCATCTACGACAACATCGCTTTTGGCGTGCGTCTGTTTGAGAAGCTCTCCCg < 1:2766118/69‑1 (MQ=255) gACGCCGTTCCCAATGTCCATCTACGACAACATCGCTTTTGGCGTGCGTCGGTTTGAGAAGCTCTCCCg < 1:2393645/69‑1 (MQ=255) gTTTCCGATGTCCATCTACGACAACATCGCTTTTGGCGTTCGTCTGTTTGAGAAGCTCTCCCGTGCCGa < 1:495040/69‑1 (MQ=255) gTTTCCGATGTCCATCTACGACAACATCGCTTTTGGCGTTCGTCTGTTTGAGAAGCTCTCCCGTGCCGa < 1:2233484/69‑1 (MQ=255) gTTTCCGATGTCCATCTACGACAACATCGCTTTTGGCGTTCGTCTGTTTGAGAAGCTCTCCCGTGCCGa < 1:580950/69‑1 (MQ=255) gTTTCCGATGTCCATCTACGACAACATCGCTTTTGGCGTTCGTCTGTTTGAGAAGCTCTCCCGTGCCGa < 1:3077737/69‑1 (MQ=255) gTTTCCGATGTCCATCTACGACAACATCGCTTTTGGCGTTCGTCTGTTTGAGAAGCTCTCCCGTGCCGa < 1:3387857/69‑1 (MQ=255) gTTTCCGATGTCCATCTACGACAACATCGCTTTTGGCGTTCGTCTGTTTGAGAAGCTCTCCCGTGCCGa < 1:1164703/69‑1 (MQ=255) gTTTCCGATGTCCATCTACGACAACATCGCTTTTGGCGTTCGTCTGTTTGAGAAGCTCTCCCGTGCCGa < 1:3361053/69‑1 (MQ=255) gTTTCCGATGTCCATCTACGACAACATCGCTTTTGGCGTTCGTCTGTTTGAGAAGCTCTCCCGTGCCGa < 1:835929/69‑1 (MQ=255) tttCCGATGTCCATCTACGACAACATCGCTTTTGGCGTTCGTCTGTTTGAGAAGCTCTCCCGTGCCGa < 1:1076722/68‑1 (MQ=255) tCCGATGTCCATCTACGACAACATCGCTTTTGGCGTTCGTCTGTTTGAGAAGCTCTCCCGTGCCGACa > 1:191237/1‑68 (MQ=255) tCCGATGTCCATCTACGACAACATCGCTTTTGGCGTTCGTCTGTTTGAGAAGCTCTCCCGTGCCGACa > 1:64761/1‑68 (MQ=255) tCCGATGTCCATCTACGACAACATCGCTTTTGGCGTTCGTCTGTTTGAGAAGCTCTCCCGTGCCGACa > 1:445817/1‑68 (MQ=255) tCCGATGTCCATCTACGACAACATCGCTTTTGGCGTTCGTCTGTTTGAGAAGCTCTCCCGTGCCGACa > 1:3338946/1‑68 (MQ=255) | AACTCTCAGGATATCGCACTGCTGCGTGCGAAAGTGGGCATGGTGTTCCAGAAACCGACGCCGTTTCCGATGTCCATCTACGACAACATCGCTTTTGGCGTTCGTCTGTTTGAGAAGCTCTCCCGTGCCGACA > minE/2478271‑2478403 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |