| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,479,154 | G→A | 23.8% | A113A (GCG→GCA) | phoU → | DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,479,154 | 0 | G | A | 23.8% | 97.5 / 22.5 | 75 | A113A (GCG→GCA) | phoU | DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (19/38); new base A (6/12); total (25/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.84e-01 | |||||||||||
TAAAACCATTGCCGAGCTGGAGCGTATTGGCGACGTGGCGGACAAAATCTGCCGTACTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCTGGTAAGTCTGGAGTCGCTGGGCCGTCAT > minE/2479094‑2479217 | tAAAACCATTGCCGAGCTGGAGCGTATTGGCGACGTGGCGGACAAAATCTGCCGTACTGCGCTGgaga < 1:244354/68‑1 (MQ=255) aaaCCATTGCCGAGCTGGAGCGTATTGGCGACGTGGCGGACAAAATCTGCCGTACTGCGCTGGAGaaa > 1:1039069/1‑68 (MQ=255) aaaCCATTGCCGAGCTGGAGCGTATTGGCGACGTGGCGGACAAAATCTGCCGTACTGCGCTGGAGAAAt > 1:1349356/1‑69 (MQ=255) aaaCCATTGCCGAGCTGGAGCGTATTGGCGACGTGGCGGACAAAATCTGCCGTACTGCGCTGGAGAAAt > 1:1541149/1‑69 (MQ=255) aaaCCATTGCCGAGCTGGAGCGTATTGGCGACGTGGCGGACAAAATCTGCCGTACTGCGCTGGAGAAAt > 1:2481336/1‑69 (MQ=255) aaaCCATTGCCGAGCTGGAGCGTATTGGCGACGTGGCGGACAAAATCTGCCGTACTGCGCTGGAGAAAt > 1:2709044/1‑69 (MQ=255) aaaCCATTGCCGAGCTGGAGCGTATTGGCGACGTGGCGGACAAAATCTGCCGTACTGCGCTGGAGAAAt > 1:1115380/1‑69 (MQ=255) gCCGAGCTGGAGCGTATTGGCGACGTGGCGGACAAAATCTGCCGTACTGCGCTGGAGAAATTCTccc > 1:2990508/1‑67 (MQ=255) gCCGAGCTGGAGCGTATTGGCGACGTGGCGGACAAAATCTGCCGTACTGCGCTGGAGAAATTCTccc > 1:2988807/1‑67 (MQ=255) gCCGAGCTGGAGCGTATTGGCGACGTGGCGGACAAAATCTGCCGTACTGCGCTGGAGAAATTCTccc > 1:428341/1‑67 (MQ=255) gCCGAGCTGGAGCGTATTGGCGACGTGGCGGACAAAATCTGCCGTACTGCGCTGGAGAAATTCTccc > 1:547567/1‑67 (MQ=255) gCCGAGCTGGAGCGTATTGGCGACGTGGCGGACAAAATCTGCCGTACTGCGCTGGAGAAATTCTccc > 1:1775180/1‑67 (MQ=255) gCCGAGCTGGAGCGTATTGGCGACGTGGCGGACAAAATCTGCCGTACTGCGCTGGAGAAATACTccc > 1:3062528/1‑67 (MQ=255) aGCTGGAGCGTATTGGCGACGTGGCGGACAAAATCTGCCGTACTGCGCTGGAGAAATTCTCCCagcagc < 1:3169869/69‑1 (MQ=255) aGCTGGAGCGTATTGGCGACGTGGCGGACAAAATCTGCCGTACTGCGCTGGAGAAATTCTCCCagcagc < 1:2643911/69‑1 (MQ=255) aGCTGGAGCGTATTGGCGACGTGGCGGACAAAATCTGCCGTACTGCGCTGGAGAAATTCTCCCagcagc < 1:276698/69‑1 (MQ=255) aGCTGGAGCGTATTGGCGACGTGGCGGACAAAATCTGCCGTACTGCGCTGGAGAAATTCTCCCagcagc < 1:604429/69‑1 (MQ=255) aGCTGGAGCGTATTGGCGACGTGGCGGACAAAATCTGCCGTACTGCGCTGGAGAAATTCTCCCagcagc < 1:1989831/69‑1 (MQ=255) aGCTGGAGCGTATTGGCGACGTGGCGGACAAAATCTGCCGTACTGCGCTGGAGAAATTCTCCCagcagc < 1:1946244/69‑1 (MQ=255) aGCTGGAGCGTATTGGCGACGTGGCGGACAAAATCTGCCGTACTGCGCTGGAGAAATTCTCCCagcagc < 1:1742999/69‑1 (MQ=255) aGCTGGAGCGTATTGGCGACGTGGCGGACAAAATCTGCCGTACTGCGCTGGAGAAATTCTCCCagcagc < 1:1287992/69‑1 (MQ=255) aGCTGGAGCGTATTGGCGACGTGGCGGACAAAATCTGCCGTACTGCGCTGGAGAAATTCTCCCagcagc < 1:1346193/69‑1 (MQ=255) aGCTGGAGCGTATTGGCGACGTGGAGGACAAAATCTGCCGTACTGCGCTGGAGAAATTCTCCCagcagc < 1:77141/69‑1 (MQ=255) gCTGGAGCGTATTGGCGACGTGGCGGACAAAATCTGCCGTACTGCGCTGGAGAAATTCTCCCagcagc < 1:2654266/68‑1 (MQ=255) gCGTATTGGCGACGTGGCGGACAAAATCTGCCGTACTGCACTTGAGAAAtt < 1:3308700/51‑1 (MQ=255) gCGTATTGGCGACGTGGCGGACAAAATCTGCCGTACTGCACTGGAGAAAtt < 1:2776886/51‑1 (MQ=255) gCGTATTGGCGACGTGGCGGACAAAATCTGCCGTACTGCACTGGAGAAAtt < 1:335393/51‑1 (MQ=255) gCGTATTGGCGACGTGGCGGACAAAATCTGCCGTACTGCACTGGAGAAAtt < 1:684239/51‑1 (MQ=255) gCGTATTGGCGACGTGGCGGACAAAATCTGCCGTACTGCACTGGAGAAAtt < 1:3277864/51‑1 (MQ=255) gCGTATTGGCGACGTGGCGGACAAAATCTGCCGTACTGCACTGGAGAAAtt < 1:305730/51‑1 (MQ=255) gCGTATTGGCGACGTGGCGGACAAAATCTGCCGTACTGCACTGGAGAAAtt < 1:2923977/51‑1 (MQ=255) gCGTATTGGCGACGTGGCGGACAAAATCTGCCGTACTGCACTGGAGAAAtt < 1:701667/51‑1 (MQ=255) gCGTATTGGCGACGTGGCGGACAAAATCTGCCGTACTGCACTGGAGAAAtt < 1:1025019/51‑1 (MQ=255) gCGTATTGGCGACGTGGCGGACAAAATCTGCCGTACTGCACTGGAGAAAtt < 1:1606906/51‑1 (MQ=255) gCGTATTGGCGACGTGGCGGACAAAATCTGCCGTACTGCACTGGAGAAAtt < 1:1802003/51‑1 (MQ=255) gCGTATTGGCGACGTGGCGGACAAAATCTGCCGTACTGCACTGGAGAAAtt < 1:2029959/51‑1 (MQ=255) gTATTGGCGACGTGGCGGACAAAATCTGCCGTACTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGt < 1:3350322/69‑1 (MQ=255) ggCGACGTTGCGGACAAAATCTGCCGTACTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCTg < 1:2234054/69‑1 (MQ=255) ggCGACGTGGCGGACAAAATCTGCCGTACTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGc > 1:387198/1‑67 (MQ=255) ggCGACGTGGCGGACAAAATCTGCCGTACTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCt > 1:1450014/1‑68 (MQ=255) ggCGACGTGGCGGACAAAATCTGCCGTACTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCt > 1:770540/1‑68 (MQ=255) ggCGACGTGGCGGACAAAATCTGCCGTACTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCt > 1:2322971/1‑68 (MQ=255) ggCGACGTGGCGGACAAAATCTGCCGTACTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCt > 1:3300391/1‑68 (MQ=255) ggCGACGTGGCGGACAAAATCTGCCGTACTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCt > 1:1352993/1‑68 (MQ=255) ggCGACGTGGCGGACAAAATCTGCCGTACTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCTg < 1:582551/69‑1 (MQ=255) ggCGACGTGGCGGACAAAATCTGCCGTACTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCTg < 1:3436101/69‑1 (MQ=255) cGTGGCGGACAAAATCTGCCGTACTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCTGGTAAg < 1:56639/69‑1 (MQ=255) cGTGGCGGACAAAATCTGCCGTACTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCTGGTAAg < 1:1621491/69‑1 (MQ=255) cGTGGCGGACAAAATCTGCCGTACTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCTGGTAAg < 1:2111993/69‑1 (MQ=255) cGTGGCGGACAAAATCTGCCGTACTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCTGGTAAg < 1:2833099/69‑1 (MQ=255) gTGGCGGACAAAATCTGCCGTACTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCTGGTAAg < 1:2884828/68‑1 (MQ=255) gTGGCGGACAAAATCTGCCGTACTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCTGGTAAg < 1:2702495/68‑1 (MQ=255) aCAAAATCTGCCGTACTGCACTGGAGAAATTCTCCCAGCAGCATCa > 1:1305993/1‑46 (MQ=255) aCAAAATCTGCCGTACTGCACTGGAGAAATTCTCCCAGCAGCATCAGCCATTGCTGGTAAGTCTGGAg > 1:1894821/1‑68 (MQ=255) aCAAAATCTGCCGTACTGCACTGGAGAAATTCTCCCAGCAGCATCAGCCATTGCTGGTAAGTCTGGAGt > 1:2615109/1‑69 (MQ=255) aCAAAATCTGCCGTACTGCACTGGAGAAATTCTCCCAGCAGCATCAGCCATTGCTGGTAAGTCTGGAGt > 1:1627013/1‑69 (MQ=255) aCAAAATCTGCCGTACTGCACTGGAGAAATTCTCCCAGCAGCATCAGCCATTGCTGGTAAGTCTGGAGt > 1:1899125/1‑69 (MQ=255) aCAAAATCTGCCGTACTGCACTGGAGAAATTCTCCCAGCAGCATCAGCCATTGCTGGTAAGTCTGGAGt > 1:679757/1‑69 (MQ=255) cAAAATCTGCCGTACTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCTGGTAAGTCTGGAGTc < 1:3005602/69‑1 (MQ=255) cAAAATCTGCCGTACTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCTGGTAAGTCTGGAGTc < 1:624877/69‑1 (MQ=255) cAAAATCTGCCGTACTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCTGGTAAGTCTGGAGTc < 1:1414510/69‑1 (MQ=255) cAAAATCTGCCGTACTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCTGGTAAGTCTGGAGTc < 1:1815126/69‑1 (MQ=255) cTGCCGTACTCCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCTg < 1:2530073/49‑1 (MQ=255) gTACTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCTGGTAAGTCTGGAGTCGCTGGGc < 1:1169420/65‑1 (MQ=255) tACTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCTTGTAAg < 1:2924973/48‑1 (MQ=255) cTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCTGGTAAGTCTg < 1:861149/50‑1 (MQ=255) cTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCTGGTAAGTCTg < 1:558626/50‑1 (MQ=255) cTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCTGGTAAGTCTg < 1:1202794/50‑1 (MQ=255) cTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCTGGTAAGTCTg < 1:1231437/50‑1 (MQ=255) cTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCTGGTAAGTCTg < 1:3350696/50‑1 (MQ=255) cTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCTGGTAAGTCTg < 1:1376668/50‑1 (MQ=255) cTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCTGGTAAGTCTg < 1:2379838/50‑1 (MQ=255) cTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCTGGTAAGTCTg < 1:2188515/50‑1 (MQ=255) cTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCTGGTAAGTCTg < 1:1643648/50‑1 (MQ=255) cTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCTGGTAAGTCTGGAGTCGCTGGGCCGTCAt > 1:3421268/1‑68 (MQ=255) | TAAAACCATTGCCGAGCTGGAGCGTATTGGCGACGTGGCGGACAAAATCTGCCGTACTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCTGGTAAGTCTGGAGTCGCTGGGCCGTCAT > minE/2479094‑2479217 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |