| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,479,184 | G→A | 27.3% | P123P (CCG→CCA) | phoU → | DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,479,184 | 0 | G | A | 27.3% | 68.5 / 22.8 | 62 | P123P (CCG→CCA) | phoU | DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (9/36); new base A (5/12); total (14/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.02e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTATTGGCGACGTGGCGGACAAAATCTGCCGTACTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCTGGTAAGTCTGGAGTCGCTGGGCCGTCATACCATCCAGATGCTGCACGACGTGCTGGACGCGTT > minE/2479117‑2479252 | gTATTGGCGACGTGGCGGACAAAATCTGCCGTACTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGt < 1:3350322/69‑1 (MQ=255) ggCGACGTTGCGGACAAAATCTGCCGTACTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCTg < 1:2234054/69‑1 (MQ=255) ggCGACGTGGCGGACAAAATCTGCCGTACTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGc > 1:387198/1‑67 (MQ=255) ggCGACGTGGCGGACAAAATCTGCCGTACTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCt > 1:770540/1‑68 (MQ=255) ggCGACGTGGCGGACAAAATCTGCCGTACTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCt > 1:2322971/1‑68 (MQ=255) ggCGACGTGGCGGACAAAATCTGCCGTACTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCt > 1:3300391/1‑68 (MQ=255) ggCGACGTGGCGGACAAAATCTGCCGTACTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCt > 1:1450014/1‑68 (MQ=255) ggCGACGTGGCGGACAAAATCTGCCGTACTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCt > 1:1352993/1‑68 (MQ=255) ggCGACGTGGCGGACAAAATCTGCCGTACTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCTg < 1:3436101/69‑1 (MQ=255) ggCGACGTGGCGGACAAAATCTGCCGTACTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCTg < 1:582551/69‑1 (MQ=255) cGTGGCGGACAAAATCTGCCGTACTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCTGGTAAg < 1:2833099/69‑1 (MQ=255) cGTGGCGGACAAAATCTGCCGTACTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCTGGTAAg < 1:1621491/69‑1 (MQ=255) cGTGGCGGACAAAATCTGCCGTACTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCTGGTAAg < 1:56639/69‑1 (MQ=255) cGTGGCGGACAAAATCTGCCGTACTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCTGGTAAg < 1:2111993/69‑1 (MQ=255) gTGGCGGACAAAATCTGCCGTACTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCTGGTAAg < 1:2884828/68‑1 (MQ=255) gTGGCGGACAAAATCTGCCGTACTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCTGGTAAg < 1:2702495/68‑1 (MQ=255) aCAAAATCTGCCGTACTGCACTGGAGAAATTCTCCCAGCAGCATCAGCCATTGCTGGTAAGTCTGGAg > 1:1894821/1‑68 (MQ=255) aCAAAATCTGCCGTACTGCACTGGAGAAATTCTCCCAGCAGCATCAGCCATTGCTGGTAAGTCTGGAGt > 1:1899125/1‑69 (MQ=255) aCAAAATCTGCCGTACTGCACTGGAGAAATTCTCCCAGCAGCATCAGCCATTGCTGGTAAGTCTGGAGt > 1:2615109/1‑69 (MQ=255) aCAAAATCTGCCGTACTGCACTGGAGAAATTCTCCCAGCAGCATCAGCCATTGCTGGTAAGTCTGGAGt > 1:679757/1‑69 (MQ=255) aCAAAATCTGCCGTACTGCACTGGAGAAATTCTCCCAGCAGCATCAGCCATTGCTGGTAAGTCTGGAGt > 1:1627013/1‑69 (MQ=255) cAAAATCTGCCGTACTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCTGGTAAGTCTGGAGTc < 1:624877/69‑1 (MQ=255) cAAAATCTGCCGTACTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCTGGTAAGTCTGGAGTc < 1:3005602/69‑1 (MQ=255) cAAAATCTGCCGTACTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCTGGTAAGTCTGGAGTc < 1:1414510/69‑1 (MQ=255) cAAAATCTGCCGTACTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCTGGTAAGTCTGGAGTc < 1:1815126/69‑1 (MQ=255) cTGCCGTACTCCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCTg < 1:2530073/49‑1 (MQ=255) gTACTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCTGGTAAGTCTGGAGTCGCTGGGc < 1:1169420/65‑1 (MQ=255) tACTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCTTGTAAg < 1:2924973/48‑1 (MQ=255) cTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCTGGTAAGTCTg < 1:1202794/50‑1 (MQ=255) cTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCTGGTAAGTCTg < 1:558626/50‑1 (MQ=255) cTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCTGGTAAGTCTg < 1:2379838/50‑1 (MQ=255) cTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCTGGTAAGTCTg < 1:2188515/50‑1 (MQ=255) cTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCTGGTAAGTCTg < 1:861149/50‑1 (MQ=255) cTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCTGGTAAGTCTg < 1:1643648/50‑1 (MQ=255) cTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCTGGTAAGTCTg < 1:1231437/50‑1 (MQ=255) cTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCTGGTAAGTCTg < 1:3350696/50‑1 (MQ=255) cTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCTGGTAAGTCTg < 1:1376668/50‑1 (MQ=255) cTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCTGGTAAGTCTGGAGTCGCTGGGCCGTCAt > 1:3421268/1‑68 (MQ=255) cTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCTGGTAAGTCTGGAGTCGCTGGGCCGTCATACCa < 1:1008898/67‑1 (MQ=255) cTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCTGGTAAGTCTGGAGTCGCTGGGCCGTCATACCa < 1:1746646/67‑1 (MQ=255) cagcaTCAGCCGTTGCTGGTAAGTCTGGAGTCGCTGGGCCGTCATACCATCCAGATGCTGCACGACGTg < 1:2854598/69‑1 (MQ=255) cagcaTCAGCCGTTGCTGGTAAGTCTGGAGTCGCTGGGCCGTCATACCATCCAGATGCTGCACGACGTg < 1:2645672/69‑1 (MQ=255) cagcaTCAGCCGTTGCTGGTAAGTCTGGAGTCGCTGGGCCGTCATACCATCCAGATGCTGCACGACGTg < 1:2395702/69‑1 (MQ=255) cagcaTCAGCCGTTGCTGGTAAGTCTGGAGTCGCTGGGCCGTCATACCATCCAGATGCTGCACGACGTg < 1:2190338/69‑1 (MQ=255) gcaTCAGCCATTGCTGGTAAGTCTGGAGTCGCTGGGCCGTCATACCATCCAAATGCTGCACGACGTGCt < 1:527087/69‑1 (MQ=255) gcaTCAGCCATTGCTGGTAAGTCTGGAGTCGCTGGGCCGTCATACCATCCAAATGCTGCACGACGTGCt < 1:416150/69‑1 (MQ=255) gcaTCAGCCATTGCTGGTAAGTCTGGAGTCGCTGGGCCGTCATACCATCCAAATGCTGCACGACGTGCt < 1:3332290/69‑1 (MQ=255) gcaTCAGCCATTGCTGGTAAGTCTGGAGTCGCTGGGCCGTCATACCATCCAAATGCTGCACGACGTGCt < 1:2752855/69‑1 (MQ=255) gcaTCAGCCATTGCTGGTAAGTCTGGAGTCGCTGGGCCGTCATACCATCCAAATGCTGCACGACGTGCt < 1:237557/69‑1 (MQ=255) gcaTCAGCCATTGCTGGTAAGTCTGGAGTCGCTGGGCCGTCATACCATCCAAATGCTGCACGACGTGCt < 1:162985/69‑1 (MQ=255) gcaTCAGCCATTGCTGGTAAGTCTGGAGTCGCTGGGCCGTCATACCATCCAAATGCTGCACGACGTGCt < 1:793074/69‑1 (MQ=255) caTCAGCCGTTGCTGGTAAGTCTGGAGTCGCTGGGCCGTCATACCATCCAGATGCTGCACGACGTGCTg < 1:11596/69‑1 (MQ=255) caTCAGCCGTTGCTGGTAAGTCTGGAGTCGCTGGGCCGTCATACCATCCAGATGCTGCACGACGTGCTg < 1:3271735/69‑1 (MQ=255) caTCAGCCGTTGCTGGTAAGTCTGGAGTCGCTGGGCCGTCATACCATCCAGATGCTGCACGACGTGCTg < 1:2701163/69‑1 (MQ=255) caTCAGCCGTTGCTGGTAAGTCTGGAGGCGCTGGGCCGTCATACCATCCAGATGCTGCACGACGTGCTg < 1:398649/69‑1 (MQ=255) caTCAGCCATTGCTGGTAAGTCTGGAGTCGCTGGGCCGTCATACCATCCAAATGCTGCACGACGTGCt < 1:2824743/68‑1 (MQ=255) caTCAGCCATTGCTGGTAAGTCTGGAGTCGCTGGGCCGTCATACCATCCAAATGCTGCACGACGTGCt < 1:1418075/68‑1 (MQ=255) caTCAGCCATTGCTGGTAAGTCTGGAGTCGCTGGGCCGTCATACCATCCAAATGCTGCACGACGTGCt < 1:92207/68‑1 (MQ=255) aTCAGCCATTGCTGGTAAGTCTGGAGTCGCTGGGCCGTCATACCATCCAAATGCTGCACGACGTGCt < 1:3122561/67‑1 (MQ=255) aTCAGCCATTGCTGGTAAGTCTGGAGTCGCTGGGCCGTCATACCATCCAAATGCTGCACGACGTGCt < 1:1208512/67‑1 (MQ=255) aGCCGTTGCTGGTAAGTCTGGAGTCGCTGGGCCGTCATACCATCCAGATGCTGCACGACGTGCTGGAcg > 1:735870/1‑69 (MQ=255) aGCCGTTGCTGGTAAGTCTGGAGTCGCTGGGCCGTCATACCATCCAGATGCTGCACGACGTGCTGGAcg > 1:1999399/1‑69 (MQ=255) gTTGCTGGTAAGTCTGGAGTCGCTGGGCCGTCATACCATCCAGATGCTGCACGACGTGCTGGACGCGtt < 1:2024967/69‑1 (MQ=255) | GTATTGGCGACGTGGCGGACAAAATCTGCCGTACTGCGCTGGAGAAATTCTCCCAGCAGCATCAGCCGTTGCTGGTAAGTCTGGAGTCGCTGGGCCGTCATACCATCCAGATGCTGCACGACGTGCTGGACGCGTT > minE/2479117‑2479252 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |