| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,480,719 | T→C | 35.3% | intergenic (‑110/+35) | yieE ← / ← yidZ | predicted phosphopantetheinyl transferase/predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,480,719 | 0 | T | C | 35.3% | 89.2 / 79.0 | 130 | intergenic (‑110/+35) | yieE/yidZ | predicted phosphopantetheinyl transferase/predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (33/51); new base C (28/18); total (61/69) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.70e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.20e-01 | |||||||||||
Only 100 of 150 total aligned reads displayed.
AATGATGGACAGACTAAAGCGGCTAAAAGTTCGATTCAGACAAGCTTATTACAAC‑TTTTTTACATTTTATTGTGAAATTGGGTCGCGTTTCATACGATTCGGTTATGCCATCGACGCGTAAAGGTTTTTAATG > minE/2480652‑2480784 | aaTGATGGACAGACTAAAGCGGCTAAAAGTTCGATTCAGACAAGCTTATTACAAC‑TTTTTTACAtttt > 1:395073/1‑68 (MQ=255)aaTGATGGACAGACTAAAGCGGCTAAAAGTTCGATTCAGACAAGCTTATTACAAC‑TTTTTTACATTTTa > 1:691696/1‑69 (MQ=255) cAGACTAAAGCGGCTAAAAGTTCGATTCAGACAAGCTTATTACAACTTTTTTTACATTTTATTGTGaaa > 1:430386/1‑69 (MQ=255) cAGACTAAAGCGGCTAAAAGTTCGATTCAGACAAGCTTATTACAACTTTTTTTACATTTTATTGTGaaa > 1:2972121/1‑69 (MQ=255) aCTAAAGCGGCTAAAAGTTCGATTCAGACAAGCTTATTACAAC‑TTTTTTACATTTTATTGTGAAATTgg > 1:2863880/1‑69 (MQ=255) cTAAAGCGGCTAAAAGTTCGATTCAGACAAGCTTATTACAAC‑TTTTTTACATTTTATTGTGAAATTggg > 1:1153933/1‑69 (MQ=255) tAAAGCGGCTAAAAGTTCGATTCAGACAAGCTTATTACAAC‑TTTTTTACAtttt > 1:2489012/1‑54 (MQ=255) tAAAGCGGCTAAAAGTTCGATTCAGACAAGCTTATTACAAC‑TTTTTTACATTTTATTGTGAAATTggg > 1:2891607/1‑68 (MQ=255) tAAAGCGGCTAAAAGTTCGATTCAGACAAGCTTATTACAAC‑TTTTTTACATTTTATTGTGAAATTGGGt > 1:3357252/1‑69 (MQ=255) tAAAGCGGCTAAAAGTTCGATTCAGACAAGCTTATTACAAC‑TTTTTTACATTTTATTGTGAAATTGGGt > 1:527461/1‑69 (MQ=255) tAAAGCGGCTAAAAGTTCGATTCAGACAAGCTTATTACAAC‑TTTTTTACATTTTATTGTGAAATTGGGt > 1:663227/1‑69 (MQ=255) gCTAAAAGTTCGATTCAGACAAGCTTATTACAAC‑TTTTTTACATTTTATTGTGAAATTGGGTCGCGttt < 1:655921/69‑1 (MQ=255) gCTAAAAGTTCGATTCAGACAAGCTTATTACAAC‑TTTTTTACATTTTATTGTGAAATTGGGTCGCGttt < 1:231996/69‑1 (MQ=255) gCTAAAAGTTCGATTCAGACAAGCTTATTACAAC‑TTTTTTACATTTTATTGTGAAATTGGGTCGCGttt < 1:1820689/69‑1 (MQ=255) gCTAAAAGTTCGATTCAGACAAGCTTATTACAAC‑TTTTTTACATTTTATTGTGAAATTGGGTCGCGttt < 1:1720578/69‑1 (MQ=255) gCTAAAAGTTCGATTCAGACAAGCTTATTACAAC‑TTTTTTACATTTTATTGTGAAATTAGGTCGCGttt < 1:2719284/69‑1 (MQ=255) cTAAAAGTTGGATTCAGACAAGCTTATTACAAC‑TTTTTTACATTTTATTGTGAAATTGGGTCGCGttt < 1:205490/68‑1 (MQ=255) cTAAAAGTTCGATTCAGACAAGCTTATTACAAC‑TTTTTTACATTTTATTGTGAAATTGGGTCGCGttt < 1:1076783/68‑1 (MQ=255) tAAAAGTTCGATTCAGACAAGCTTATTACAAC‑TTTTTTACATTTTATTGTGAAATTGGGTCGCGTTTCa < 1:381150/69‑1 (MQ=255) tAAAAGTTCGATTCAGACAAGCTTATTACAAC‑TTTTTTACATTTTATTGTGAAATTGGGTCGCGTTTCa < 1:1888681/69‑1 (MQ=255) tAAAAGTTCGATTCAGACAAGCTTATTACAAC‑TTTTTTACATTTTATTGTGAAATTGGGTCGCGTTTCa < 1:2234116/69‑1 (MQ=255) aaaaGTTCGATTCAGACAAGCTTATTACAAC‑TTTTTTACATTTTATTGTGAAATTGGGTCGCGTTTCa < 1:1636512/68‑1 (MQ=255) gTTCGATTCAGATAAGTTTATTACAAC‑TTTTTTACATTTCATTGTGAAATTGGGTCGCGTTTCATACGa < 1:100366/69‑1 (MQ=255) gTTCGATTCAGATAAGTTTATTACAAC‑TTTTTTACATTTCATTGTGAAATTGGGTCGCGTTTCATACGa < 1:596959/69‑1 (MQ=255) gTTCGATTCAGATAAGTTTATTACAAC‑TTTTTTACATTTCATTGTGAAATTGGGTCGCGTTTCATACGa < 1:2810877/69‑1 (MQ=255) gTTCGATTCAGATAAGTTTATTACAAC‑TTTTTTACATTTCATTGTGAAATTGGGTCGCGTTTCATACGa < 1:2511447/69‑1 (MQ=255) gTTCGATTCAGATAAGTTTATTACAAC‑TTTTTTACATTTCATTGTGAAATTGGGTCGCGTTTCATACGa < 1:323515/69‑1 (MQ=255) gTTCGATTCAGATAAGTTTATTACAAC‑TTTTTTACATTTCATTGTGAAATTGGGTCGCGTTTCATACGa < 1:468673/69‑1 (MQ=255) gTTCGATTCAGATAAGTTTATTACAAC‑TTTTTTACATTTCATTGTGAAATTGGGTCGCGTTTCATACGa < 1:702004/69‑1 (MQ=255) gTTCGATTCAGATAAGTTTATTACAAC‑TTTTTTACATTTCATTGTGAAATTGGGTCGCGTTTCATACGa < 1:2368015/69‑1 (MQ=255) gTTCGATTCAGATAAGTTTATTACAAC‑TTTTTTACATTTCATTGTGAAATTGGGTCGCGTTTCATACGa < 1:89637/69‑1 (MQ=255) gTTCGATTCAGATAAGTTTATTACAAC‑TTTTTTACATTTCATTGTGAAATTGGGTCGCGTTTCATACGa < 1:1118323/69‑1 (MQ=255) gTTCGATTCAGATAAGTTTATTACAAC‑TTTTTTACATTTCATTGTGAAATTGGGTCGCGTTTCATACGa < 1:1424205/69‑1 (MQ=255) gTTCGATTCAGATAAGTTTATTACAAC‑TTTTTTACATTTCATTGTGAAATTGGGTCGCGTTTCATACGa < 1:1218002/69‑1 (MQ=255) gTTCGATTCAGATAAGTTTATTACAAC‑TTTTTTACATTTCATTGTGAAATTGGGTCGCGTTTCATACGa < 1:2005691/69‑1 (MQ=255) gTTCGATTCAGATAAGTTTATTACAAC‑TTTTTTACATTTCATTGTGAAATTGGGTCGCGTTTCATACGa < 1:1866628/69‑1 (MQ=255) gTTCGATTCAGATAAGTTTATTACAAC‑TTTTTTACATTTCATTGTGAAATTGGGTCGCGTTTCATACGa < 1:1798962/69‑1 (MQ=255) gTTCGATTCAGATAAGTTTATTACAAC‑ATTTTTACATTTCATTGTGAAATTGGGTCGCGTTTCATACGa < 1:2790852/69‑1 (MQ=255) ttCAGACAAGCTTATTACAAC‑TTTTTTACATTTTATTGTGAAATTGGGTCGCGTTTCATACGATTCGGt < 1:1518546/69‑1 (MQ=255) ttCAGACAAGCTTATTACAAC‑TTTTTTACATTTTATTGTGAAATTGGGTCGCGTTTCATACGATTCGGt < 1:2819065/69‑1 (MQ=255) ttCAGACAAGCTTATTACAAC‑TTTTTTACATTTTATTGTGAAATTGGGTCGCGTTTCATACGATTCGGt < 1:579114/69‑1 (MQ=255) ttCAGACAAGCTTATTACAAC‑TTTTTTACATTTTATTGTGAAATTGGGTCGCGTTTCATACGATTCGGt < 1:2871803/69‑1 (MQ=255) ttCAGACAAGCTTATTACAAC‑TTTTTTACATTTTATTGTGAAATTGGGTCGCGTTTCATACGATTCGGt < 1:2879384/69‑1 (MQ=255) ttCAGACAAGCTTATTACAAC‑TTTTTTACATTTTATTGTGAAATTGGGTCGCGTTTCATACGATTCGGt < 1:1359177/69‑1 (MQ=255) ttCAGACAAGCTTATTACAAC‑TTTTTTACATTTTATTGTGAAATTGGGTCGCGTTTCATACGATTCGGt < 1:2921725/69‑1 (MQ=255) ttCAGACAAGCTTATTACAAC‑TTTTTTACATTTTATTGTGAAATTGGGTCGCGTTTCATACGATTCGGt < 1:3055304/69‑1 (MQ=255) ttCAGACAAGCTTATTACAAC‑TTTTTTACATTTTATTGTGAAATTGGGTCGCGTTTCATACGATTCGGt < 1:3218841/69‑1 (MQ=255) ttCAGACAAGCTTATTACAAC‑TTTTTTACATTTTATTGTGAAATTGGGTCGCGTTTCATACGATTCGGt < 1:120849/69‑1 (MQ=255) ttCAGACAAGCTTATTACAAC‑TTTTTTACATTTTATTGTGAAATTGGGTCGCGTTTCATACGATTCGGt < 1:3414645/69‑1 (MQ=255) ttCAGACAAGCTTATTACAAC‑TTTTTTACATTTTATTGTGAAATTGGGTCGCGTTTCATACGATTCGGt < 1:2068779/69‑1 (MQ=255) ttCAGACAAGCTTATTACAAC‑TTTTTTACATTTTATTGTGAAATTGGGTCGCGTTTCATACGATTCGGt < 1:1567241/69‑1 (MQ=255) ttCAGACAAGCTTATTACAAC‑TTTTTTACATTTTATTGTGAAATTGGGTCGCGTTTCATACGATTCGGt < 1:1595472/69‑1 (MQ=255) ttCAGACAAGCTTATTACAAC‑TTTTTTACATTTTATTGTGAAATTGGGTCGCGTTTCATACGATTCGGt < 1:16082/69‑1 (MQ=255) ttCAGACAAGCTTATTACAAC‑TTTTTTACATTTTATTGTGAAATTGGGTCGCGTTTCATACGATTCGGt < 1:1655498/69‑1 (MQ=255) ttCAGACAAGCTTATTACAAC‑TTTTTTACATTTTATTGTGAAATTGGGTCGCGTTTCATACGATTCGGt < 1:729573/69‑1 (MQ=255) ttCAGACAAGCTTATTACAAC‑TTTTTTACATTTTATTGTGAAATTGGGTCGCGTTTCATACGATTCGGt < 1:2495152/69‑1 (MQ=255) ttCAGACAAGCTTATTACAAC‑TTTTTTACATTTTATTGTGAAATTGGGTCGCGTTTCATACGATTCGGt < 1:2444035/69‑1 (MQ=255) ttCAGACAAGCTTATTACAAC‑TTTTTTACATTTTATTGTGAAATTGGGTCGCGTTTCATACGATTCGGt < 1:2430574/69‑1 (MQ=255) ttCAGACAAGCTTATTACAAC‑TTTTTTACATTTTATTGTGAAATTGGGTCGCGTTTCATACGATTCGGt < 1:2166340/69‑1 (MQ=255) ttCAGACAAGCTTATTACAAC‑TTTTTTACATTTTATTGTGAAATTGGGTCGCGTTTCATACGATTCGGt < 1:2167689/69‑1 (MQ=255) ttCAGACAAGCTTATTACAAC‑TTTTTTACATTTTATTGTGAAATTGGGTCGCGTTTCATACGATTCGGt < 1:1991251/69‑1 (MQ=255) ttCAGACAAGCTTATTACAAC‑TTTTTTACATTTTATTGTGAAATTGCGTCGCGTTTCATACGATTCGGt < 1:757799/69‑1 (MQ=255) cAGACAAGCTTATTACAAC‑TTTTTTACATTTTATTGTGa > 1:532369/1‑39 (MQ=255) cAGACAAGCTTATTACAAC‑TTTTTTACATTTTATTGTGAAATTGGGTCGCGTTTCATACGATTCGGTTa > 1:1165081/1‑69 (MQ=255) aCAAGCTTATTACAAC‑TTTTTTACATTTTATTGTGAAATTGGGTCGCGTTTCATACGATTCGGTTATg < 1:42364/68‑1 (MQ=255) cAAGCTTATTACAAC‑TTTTTTACATTTTATTGTGAAATTGGGt > 1:321305/1‑43 (MQ=255) cAAGCTTATTACAAC‑TTTTTTACATTTTATTGTGAAATTGGGTCGCGTTTCATACGATTCGGTTATGcc > 1:2756688/1‑69 (MQ=255) cAAGCTTATTACAAC‑TTTTTTACATTTTATTGTGAAATTGGGTCGCGTTTCATACGATTCGGTTATGc > 1:3178191/1‑68 (MQ=255) aCAAC‑TTTTTTACATTTTATTGTGAAATTGGGTCGCGTTTCATACGATTCGGTTATGCCATCGAcgcg > 1:94642/1‑68 (MQ=255) aaC‑TTTTTTACATTTCATTGTGAAATTGGGTcgcg > 1:1871473/1‑35 (MQ=255) aaC‑TTTTTTACATTTCATTGTGAAATTGGGTCGCGTTTCa > 1:1927179/1‑40 (MQ=255) aaC‑TTTTTTACATTTCATTGTGAAATTGGGTCGCGTTTCATAc > 1:1337218/1‑43 (MQ=255) aaC‑TTTTTTACATTTCATTGTGAAATTGGGTCGCGTTTCATACg > 1:533572/1‑44 (MQ=255) aaC‑TTTTTTACATTTCATTGTGAAATTGGGTCGCGTTTCATACg > 1:779346/1‑44 (MQ=255) aaC‑TTTTTTACATTTCATTGTGAAATTGGGTCGCGTTTCATACg > 1:730261/1‑44 (MQ=255) aaC‑TTTTTTACATTTCATTGTGAAATTGGGTCGCGTTTCATACg > 1:1064612/1‑44 (MQ=255) aaC‑TTTTTTACATTTCATTGTGAAATTGGGTCGCGTTTCATACg > 1:3425904/1‑44 (MQ=255) aaC‑TTTTTTACATTTCATTGTGAAATTGGGTCGCGTTTCATACg > 1:3328797/1‑44 (MQ=255) aaC‑TTTTTTACATTTCATTGTGAAATTGGGTCGCGTTTCATACg > 1:3319182/1‑44 (MQ=255) aaC‑TTTTTTACATTTCATTGTGAAATTGGGTCGCGTTTCATACg > 1:3283814/1‑44 (MQ=255) aaC‑TTTTTTACATTTCATTGTGAAATTGGGTCGCGTTTCATACg > 1:3279953/1‑44 (MQ=255) aaC‑TTTTTTACATTTCATTGTGAAATTGGGTCGCGTTTCATACg > 1:3241820/1‑44 (MQ=255) aaC‑TTTTTTACATTTCATTGTGAAATTGGGTCGCGTTTCATACg > 1:1114632/1‑44 (MQ=255) aaC‑TTTTTTACATTTCATTGTGAAATTGGGTCGCGTTTCATACg > 1:313762/1‑44 (MQ=255) aaC‑TTTTTTACATTTCATTGTGAAATTGGGTCGCGTTTCATACg > 1:2979763/1‑44 (MQ=255) aaC‑TTTTTTACATTTCATTGTGAAATTGGGTCGCGTTTCATACg > 1:153580/1‑44 (MQ=255) aaC‑TTTTTTACATTTCATTGTGAAATTGGGTCGCGTTTCATACg > 1:2098202/1‑44 (MQ=255) aaC‑TTTTTTACATTTCATTGTGAAATTGGGTCGCGTTTCATACg > 1:2771356/1‑44 (MQ=255) aaC‑TTTTTTACATTTCATTGTGAAATTGGGTCGCGTTTCATACg > 1:2144720/1‑44 (MQ=255) aaC‑TTTTTTACATTTCATTGTGAAATTGGGTCGCGTTTCATACg > 1:2561055/1‑44 (MQ=255) ttATTGTGAAATTGGGTCGCGTTTCATACGATTCGGt < 1:1082229/37‑1 (MQ=255) ttATTGTGAAATTGGGTCGCGTTTCATACGATTCGGt < 1:3379262/37‑1 (MQ=255) ttATTGTGAAATTGGGTCGCGTTTCATACGATTCGGt < 1:1417797/37‑1 (MQ=255) ttATTGTGAAATTGGGTCGCGTTTCATACGATTCGGt < 1:2633510/37‑1 (MQ=255) ttATTGTGAAATTGGGTCGCGTTTCATACGATTCGGt < 1:2790712/37‑1 (MQ=255) ttATTGTGAAATTGGGTCGCGTTTCATACGATTCGGt < 1:3101691/37‑1 (MQ=255) ttATTGTGAAATTGGGTCGCGTTTCATACGATTCGGTTATGCCATCGACGCGTAAAGGTTTTTAAt < 1:2382195/66‑1 (MQ=255) ttATTGTGAAATTGGGTCGCGTTTCATACGATTCGGTTATGCCATCGACGCGTAAAGGTTTTTAAt < 1:285737/66‑1 (MQ=255) ttATTGTGAAATTGGGTCGCGTTTCATACGATTCGGTTATGCCATCGACGCGTAAAGGTTTTTAAt < 1:488038/66‑1 (MQ=255) ttATTGTGAAATTGGGTCGCGTTTCATACGATTCGGTTATGCCATCGACGCGTAAAGGTTTTTAAt < 1:449417/66‑1 (MQ=255) | AATGATGGACAGACTAAAGCGGCTAAAAGTTCGATTCAGACAAGCTTATTACAAC‑TTTTTTACATTTTATTGTGAAATTGGGTCGCGTTTCATACGATTCGGTTATGCCATCGACGCGTAAAGGTTTTTAATG > minE/2480652‑2480784 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |