| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 209,373 | T→C | 50.7% | L111L (TTA→TTG) | yafC ← | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 209,373 | 0 | T | C | 50.7% | ‑1.3 / 62.8 | 71 | L111L (TTA→TTG) | yafC | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (27/8); new base C (6/30); total (33/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.14e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.04e-01 | |||||||||||
TTAATAATCGTTTCGGAGGAGACTAGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCATCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACAGTCCACGCG > minE/209305‑209434 | ttAATAATCGTTTCGGAGGAGACTAGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCttaatt < 1:1163118/69‑1 (MQ=255) taatCGTTTCGGAGGAGACTAGCGATAAAGTGACTTCCGGATAGCGTTCACGAAAAGGCTTAATCAAc < 1:1152451/68‑1 (MQ=255) aatCGTTTCGGAGGAGACTAGCGATAAAGTGACTTCCGGATAGCGTTCACGAAAAGGCTTAATCAAc < 1:1013566/67‑1 (MQ=255) aatCGTTTCGGAGGAGACTAGCGATAAAGTGACTTCCGGATAGCGTTCACGAAAAGGCTTAATCAAc < 1:2634719/67‑1 (MQ=255) aatCGTTTCGGAGGAGACTAGCGATAAAGTGACTTCCGGATAGCGTTCACGAAAAGGCTTAATCAAc < 1:377306/67‑1 (MQ=255) aatCGTTTCGGAGGAGACTAGCGATAAAGTGACTTCCGGATAGCGTTCACGAAAAGGCTTAATCAAc < 1:654568/67‑1 (MQ=255) gaCTAGCGATAAAGTGACTTCCGGATAGCGTTCACGAAAAGGCTTAATCAACGGCATCAGAAAGTgcag > 1:1187523/1‑69 (MQ=255) gaCTAGCGATAAAGTGACTTCCGGATAGCGTTCACGAAAAGGCTTAATCAACGGCATCAGAAAGTgcag > 1:1229329/1‑69 (MQ=255) gaCTAGCGATAAAGTGACTTCCGGATAGCGTTCACGAAAAGGCTTAATCAACGGCATCAGAAAGTgcag > 1:26496/1‑69 (MQ=255) gaCTAGCGATAAAGTGACTTCCGGATAGCGTTCACGAAAAGGCTTAATCAACGGCATCAGAAAGTgcag > 1:3212568/1‑69 (MQ=255) gaCTAGCGATAAAGTGACTTCCGGATAGCGTTCACGAAAAGGCTTAATCAACGGCATCAGAAAGTgca > 1:1333605/1‑68 (MQ=255) gaCTAGCGATAAAGTGACTTCCGGATAGCGTTCACGAAAAGGCTTAATCAACGGCATCAGAAAGTgca > 1:1040679/1‑68 (MQ=255) aGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCATCAGAAAGTGCAGcacc < 1:936930/69‑1 (MQ=255) aGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCATCAGAAAGTGCAGcacc < 1:311030/69‑1 (MQ=255) aGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCATCAGAAAGTGCAGcacc < 1:410285/69‑1 (MQ=255) aGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCATCAGAAAGTGCAGcacc < 1:2220082/69‑1 (MQ=255) gTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCATCAGAAAGTGCAGCACCACTGGAGtt > 1:2911670/1‑69 (MQ=255) gTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCATCAGAAAGTGCAGCACCACTGGAGtt > 1:2018700/1‑69 (MQ=255) gTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCATCAGAAAGTGCAGCACCACTGGAGtt > 1:3262833/1‑69 (MQ=255) gTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCATCAGAAAGTGCAGCACCACTGGAGtt > 1:3306021/1‑69 (MQ=255) gTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCATCAGAAAGTGCAGCACCACTGGAGtt > 1:33154/1‑69 (MQ=255) gTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCATCAGAAAGTGCAGCACCACTGGAGtt > 1:963912/1‑69 (MQ=255) gTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCATCAGAAAGTGCAGCACCACTGGAGtt > 1:487837/1‑69 (MQ=255) gTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCATCAGAAAGTGCAGCACCACTGGAGtt > 1:1121030/1‑69 (MQ=255) aCTTCCGGATATCGTTCACGAAAAGGCTTAATCAACGGCATCAGAAAGTGCAGCACCACTGGAGTTGCg < 1:155184/69‑1 (MQ=255) aCTTCCGGATAGCGTTCACGAAAAGGCTTAATCAACGGCATCAGAAAGTGCAGCACCACTGGAGTTGCg < 1:2794134/69‑1 (MQ=255) aCTTCCGGATAGCGTTCACGAAAAGGCTTAATCAACGGCATCAGAAAGTGCAGCACCACTGGAGTTGCg < 1:1018506/69‑1 (MQ=255) aCTTCCGGATAGCGTTCACGAAAAGGCTTAATCAACGGCATCAGAAAGTGCAGCACCACTGGAGTTGCg < 1:2486163/69‑1 (MQ=255) aCTTCCGGATAGCGTTCACGAAAAGGCTTAATCAACGGCATCAGAAAGTGCAGCACCACTGGAGTTGCg < 1:3015857/69‑1 (MQ=255) aCTTCCGGATAGCGTTCACGAAAAGGCTTAATCAACGGCATCAGAAAGTGCAGCACCACTGGAGTTGCg < 1:3321795/69‑1 (MQ=255) aCTTCCGGATAGCGTTCACGAAAAGGCTTAATCAACGGCATCAGAAAGTGCAGCACCACTGGAGTTGCg < 1:3433369/69‑1 (MQ=255) aCTTCCGGATAGCGTTCACGAAAAGGCTTAATCAACGGCATCAGAAAGTGCAGCACCACTGGAGTTGCg < 1:56830/69‑1 (MQ=255) aCTTCCGGATAGCGTTCACGAAAAGGCTTAATCAACGGCATCAGAAAGTGCAGCACCACTGGAGTTGCg < 1:681001/69‑1 (MQ=255) aCTTCCGGATAGCGTTCACGAAAAGGCTTAATCAACGGCATCAGAAAGTGCAGCACCACTGGAGTTGCg < 1:731244/69‑1 (MQ=255) aCTTCCGGATAGCGTTCACGAAAAGGCTTAATCAACGGCATCAGAAAGTGCAGCACCACTGGAGTTGCg < 1:135504/69‑1 (MQ=255) aCTTCCGGATAGCGTTCACGAAAAGGCTTAATCAACGGCATCAGAAAGTGCAGCACCACTGGAGTTGCg < 1:1409816/69‑1 (MQ=255) aCTTCCGGATAGCGTTCACGAAAAGGCTTAATCAACGGCATCAGAAAGTGCAGCACCACTGGAGTTGCg < 1:157128/69‑1 (MQ=255) aCTTCCGGATAGCGTTCACGAAAAGGCTTAATCAACGGCATCAGAAAGTGCAGCACCACTGGAGTTGCg < 1:2412759/69‑1 (MQ=255) aCTTCCGGATAGCGTTCACGAAAAGGCTTAATCAACGGCATCAGAAAGTGCAGCACCACTGGAGTTGCg < 1:215074/69‑1 (MQ=255) aCTTCCGGATAGCGTTCACGAAAAGGCTTAATCAACGGCATCAGAAAGTGCAGCACCACTGGAGTTGCg < 1:1749720/69‑1 (MQ=255) aCTTCCGGATAGCGTTCACGAAAAGGCTTAATCAACGGCATCAGAAAGTGCAGCACCACTGGAGTTGCg < 1:1803311/69‑1 (MQ=255) aCTTCCGGATAGCGTTCACGAAAAGGCTTAATCAACGGCATCAGAAAGTGCAGCACCACTGGAGTTGCg < 1:1863007/69‑1 (MQ=255) aCTTCCGGATAGCGTTCACGAAAAGGCTTAATCAACGGCATCAGAAAGTGCAGCACCACTGGAGTTGCg < 1:2064912/69‑1 (MQ=255) aCTTCCGGATAGCGTTCACGAAAAGGCTTAATCAACGGCATCAGAAAGTGCAGCACCACTGGAGTTGCg < 1:1993744/69‑1 (MQ=255) aCTTCCGGATAGCGTTCACGAAAAGGCTTAATCAACGGCATCAGAAAGTGCAGCACCACTGGAGATGCg < 1:353316/69‑1 (MQ=255) cTTCCGGATAGCGTTCACGAAAAGGCTTAATCAACGGCATCAGAAAGTGCAGCACCACTGGAGTTGCg < 1:1679639/68‑1 (MQ=255) cTTCCGGATAGCGTTCACGAAAAGGCTTAATCAACGGCATCAGAAAGTGCAGCACCACTGGAGTTGCg < 1:986309/68‑1 (MQ=255) cTTCCGGATAGCGTTCACGAAAAGGCTTAATCAACGGCATCAGAAAGTGCAGCACCACTGGAGTTGCg < 1:1697598/68‑1 (MQ=255) tCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCATCAGAAAGTGCAGCACCACTGGAGTTGCGGCa < 1:891330/69‑1 (MQ=255) tCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCATCAGAAAGTGCAGCACCACTGGAGTTGCGGCa < 1:1314802/69‑1 (MQ=255) gATAGCGTTCACGGAAAGGCTTAATTAACGGCATCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGa < 1:3369445/69‑1 (MQ=255) gATAGCGTTCACGGAAAGGCTTAATTAACGGCATCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGa < 1:2768837/69‑1 (MQ=255) cACGGAAAGGCTTAATTAACGGCATCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACa > 1:1457808/1‑69 (MQ=255) cACGGAAAGGCTTAATTAACGGCATCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACa > 1:2486560/1‑69 (MQ=255) cACGGAAAGGCTTAATTAACGGCATCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACa > 1:1023861/1‑69 (MQ=255) cACGGAAAGGCTTAATTAACGGCATCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACa > 1:881218/1‑69 (MQ=255) cACGGAAAGGCTTAATTAACGGCATCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACa > 1:1305014/1‑69 (MQ=255) cACGGAAAGGCTTAATTAACGGCATCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACa > 1:2361209/1‑69 (MQ=255) cACGGAAAGGCTTAATTAACGGCATCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACa > 1:1444865/1‑69 (MQ=255) cACGGAAAGGCTTAATTAACGGCATCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACa > 1:1487498/1‑69 (MQ=255) cACGGAAAGGCTTAATTAACGGCATCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACa > 1:2690593/1‑69 (MQ=255) cACGGAAAGGCTTAATTAACGGCATCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACa > 1:2786711/1‑69 (MQ=255) cACGGAAAGGCTTAATTAACGGCATCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACa > 1:2804840/1‑69 (MQ=255) cACGGAAAGGCTTAATTAACGGCATCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACa > 1:2818146/1‑69 (MQ=255) cACGGAAAGGCTTAATTAACGGCATCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACa > 1:3260718/1‑69 (MQ=255) cACGGAAAGGCTTAATTAACGGCATCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATACGTAACa > 1:10766/1‑69 (MQ=255) gCTTAATTAACGGCATCAGAAAGTGCAGCACCACTGGAg > 1:328469/1‑39 (MQ=255) gCTTAATTAACGGCATCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACAGTCCAcgcg > 1:2969295/1‑69 (MQ=255) gCTTAATTAACGGCATCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACAGTCCAcgcg > 1:834933/1‑69 (MQ=255) gCTTAATTAACGGCATCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACAGTCCAcgcg > 1:926675/1‑69 (MQ=255) gCTTAATTAACGGCATCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACAGTCCAcgcg > 1:2115018/1‑69 (MQ=255) cTTAATCAACGGCATCAGAAAGTGCAGCACCACTGGAGTTGCg < 1:3408584/43‑1 (MQ=255) aattaaCGGCATCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGa < 1:406807/47‑1 (MQ=255) aatcaaCGGCATCAGAAAGTGCAGCACCACTGGAGTTGCg < 1:2309038/40‑1 (MQ=255) | TTAATAATCGTTTCGGAGGAGACTAGCGACAAAGTGACTTCCGGATAGCGTTCACGGAAAGGCTTAATTAACGGCATCAGAAAGTGCAGCACCACTGGAGTTGCGGCATCGATCCGTAACAGTCCACGCG > minE/209305‑209434 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |