| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,480,838 | C→A | 18.8% | L292L (CTG→CTT) | yidZ ← | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,480,838 | 0 | C | A | 18.8% | 116.3 / 14.4 | 58 | L292L (CTG→CTT) | yidZ | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (33/14); new base A (5/6); total (38/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.63e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 | |||||||||||
TAAAGGTTTTTAATGGTTTCCCGTAACCAGACGATCTTCGGATTATGGCTGTTCCGTTTATGCCACAGCAGGGTAAAAGGAACTTCCAGCTTTTTTTGCTGGCTTTCGTCAAACGGGAGAGGAAGA > minE/2480770‑2480895 | tAAAGGTTTTTAATGGTTTCCCGTAACCAGACGATCTTCGGATTATGGCTGTTCCGTTTATGCCAcagc < 1:730213/69‑1 (MQ=255)tAAAGGTTTTTAATGGTTTCCCGTAACCAGACGATCTTCGGATTATGGCTGTTCCGTTTATGCCAcagc < 1:2282562/69‑1 (MQ=255) aaaGGTTTTTAATGGTTTCCCGTAACCAGACGATCTTCGGATTATGGCTGTTCCGTTTATGCCAcagc < 1:1892778/68‑1 (MQ=255) aaGGTTTTTAATGGTTTCCCGTAACCAGACGATCTTCGGATTATGGCTGTTCCGTTTATGCCAcagcag > 1:2516515/1‑69 (MQ=255) aaGGTTTTTAATGGTTTCCCGTAACCAGACGATCTTCGGATTATGGCTGTTCCGTTTATGCCAcagcag > 1:2546268/1‑69 (MQ=255) aaGGTTTTTAATGGTTTCCCGTAACCAGACGATCTTCGGATTATGGCTGTTCCGTTTATGCCAcagcag > 1:1791906/1‑69 (MQ=255) tGGTTTCCCGTAACCAGACGATCTTCGGATTATGGCTGTTCCGTTTATGGCACAGCAGGGTAAAAGGa < 1:2413912/68‑1 (MQ=255) tGGTTTCCCGTAACCAGACGATCTTCGGATTATGGCTGTTCCGTTTATGCCACAGCAGGGTAAAAGGa < 1:1179073/68‑1 (MQ=255) tGGTTTCCCGTAACCAGACGATCTTCGGATTATGGCTGTTCCGTTTATGCCACAGCAGGGTAAAAGGa < 1:2531365/68‑1 (MQ=255) tGGTTTCCCGTAACCAGACGATCTTCGGATTATGGCTGTTCCGTTTATGCCACAGCAGGGTAAAAGGa < 1:2781006/68‑1 (MQ=255) tGGTTTCCCGTAACCAGACGATCTTCGGATTATGGCTGTTCCGTTTATGCCACAGCAGGGTAAAAGGa < 1:286105/68‑1 (MQ=255) tGGTTTCCCGTAACCAGACGATCTTCGGATTATGGCTGTTCCGTTTATGCCACAGCAGGGTAAAAGGa < 1:2215445/68‑1 (MQ=255) tGGTTTCCCGTAACCAGACGATCTTCGGATTATGGCTGTTCCGTTTATGCCACAGCAGGGTAAAAGGa < 1:2194652/68‑1 (MQ=255) tGGTTTCCCGTAACCAGACGATCTTCGGATTATGGCTGTTCCGTTTATGCCACAGCAGGGTAAAAGGa < 1:1995002/68‑1 (MQ=255) tGGTTTCCCGTAACCAGACGATCTTCGGATTATGGCTGTTCCGTTTATGCCACAGCAGGGTAAAAGGa < 1:1697771/68‑1 (MQ=255) tGGTTTCCCGTAACCAGACGATCTTCGGATTATGGCTGTTCCGTTTATGCCACAGCAGGGTAAAAGGa < 1:1222913/68‑1 (MQ=255) ggTTTCCCGTAACCAGACGATCTTCGGATTATGGCTGTTCCGTTTATGCCACAGCAGGGTAAAAGGa < 1:684052/67‑1 (MQ=255) ggTTTCCCGTAACCAGACGATCTTCGGATTATGGCTGTTCCGTTTATGCCACAGCAGGGTAAAAGGa < 1:2715292/67‑1 (MQ=255) aCCAGACGATCTTCGGATTATGGCTGTTCCGTTTATGCCACAGCAGGGTAAAAGGAACTTcc > 1:3380724/1‑62 (MQ=255) aCCAGACGATCTTCGGATTATGGCTGTTCCGTTTATGCCACAGCAGGGTAAAAGGAACTTcc > 1:3304162/1‑62 (MQ=255) aCCAGACGATCTTCGGATTATGGCTGTTCCGTTTATGCCACAGCAGGGTAAAAGGAACTTcc > 1:3407761/1‑62 (MQ=255) aCCAGACGATCTTCGGATTATGGCTGTTCCGTTTATGCCACAGCAGGGTAAAAGGAACTTcc < 1:288195/62‑1 (MQ=255) aCCAGACGATCTTCGGATTATGGCTGTTCCGTTTATGCCACAGCAGGGTAAAAGGAACTTcc > 1:361470/1‑62 (MQ=255) aCCAGACGATCTTCGGATTATGGCTGTTCCGTTTATGCCACAGCAGGGTAAAAGGAACTTcc > 1:524699/1‑62 (MQ=255) aCCAGACGATCTTCGGATTATGGCTGTTCCGTTTATGCCACAGCAGGGTAAAAGGAACTTcc > 1:551465/1‑62 (MQ=255) aCCAGACGATCTTCGGATTATGGCTGTTCCGTTTATGCCACAGCAGGGTAAAAGGAACTTcc < 1:826629/62‑1 (MQ=255) aCCAGACGATCTTCGGATTATGGCTGTTCCGTTTATGCCACAGCAGGGTAAAAGGAACTTcc > 1:922312/1‑62 (MQ=255) aCCAGACGATCTTCGGATTATGGCTGTTCCGTTTATGCCACAGCAGGGTAAAAGGAACTTcc > 1:270516/1‑62 (MQ=255) aCCAGACGATCTTCGGATTATGGCTGTTCCGTTTATGCCACAGCAGGGTAAAAGGAACTTcc > 1:2372326/1‑62 (MQ=255) aCCAGACGATCTTCGGATTATGGCTGTTCCGTTTATGCCACAGCAGGGTAAAAGGAACTTcc > 1:1669578/1‑62 (MQ=255) aCCAGACGATCTTCGGATTATGGCTGTTCCGTTTATGCCACAGCAGGGTAAAAGGAACTTcc > 1:1673496/1‑62 (MQ=255) aCCAGACGATCTTCGGATTATGGCTGTTCCGTTTATGCCACAGCAGGGTAAAAGGAACTTcc > 1:2335817/1‑62 (MQ=255) aCCAGACGATCTTCGGATTATGGCTGTTCCGTTTATGCCACAGCAGGGTAAAAGGAACTTcc > 1:1914955/1‑62 (MQ=255) aCGATCTTCGGATTACGGCTGTTCCGTTTATGCCACAGAAGGGTAAAAgg > 1:2512016/1‑50 (MQ=255) aCGATCTTCGGATTACGGCTGTTCCGTTTATGCCACAGAAGGGTAAAAgg > 1:715453/1‑50 (MQ=255) aCGATCTTCGGATTACGGCTGTTCCGTTTATGCCACAGAAGGGTAAAAgg > 1:1637372/1‑50 (MQ=255) aCGATCTTCGGATTACGGCTGTTCCGTTTATGCCACAGAAGGGTAAAAgg > 1:895086/1‑50 (MQ=255) aCGATCTTCGGATTACGGCTGTTCCGTTTATGCCACAGAAGGGTAAAAgg > 1:1915919/1‑50 (MQ=255) ttATGGCTGTTCCGTTTATGCCACAGCAGGGTAAAAGGAACTTCCAGCTTTTTTTGCTGGCTTTCGTCa > 1:2156361/1‑69 (MQ=255) ttATGGCTGTTCCGTTTATGCCACAGCAGGGTAAAAGGAACTTCCAGCTTTTTTTGCTGGCTTTCGTCa > 1:872210/1‑69 (MQ=255) ttATGGCTGTTCCGTTTATGCCACAGCAGGGTAAAAGGAACTTCCAGCTTTTTTTGCTGGCTTTCGTCa > 1:3309059/1‑69 (MQ=255) ttATGGCTGTTCCGTTTATGCCACAGCAGGGTAAAAGGAACTTCCAGCTTTTTTTGCTGGCTTTCGTCa > 1:2785576/1‑69 (MQ=255) ttATGGCTGTTCCGTTTATGCCACAGCAGGGTAAAAGGAACTTCCAGCTTTTTTTGCTGGCTTTCGTCa > 1:2781040/1‑69 (MQ=255) ttATGGCTGTTCCGTTTATGCCACAGCAGGGTAAAAGGAACTTCCAGCTTTTTTTGCTGGCTTTCGTCa > 1:1799434/1‑69 (MQ=255) ttATGGCTGTTCCGTTTATGCCACAGCAGGGTAAAAGGAACTTCCAGCTTTTTTTGCTGGCTTTCGTCa > 1:2343468/1‑69 (MQ=255) tacggCTGTTCCGTTTATGCCACAGAAGGGTAAAAGGGACTTCCAGCTTTTTTTGCTGGCTTTCATCaa < 1:2944905/66‑1 (MQ=255) tacggCTGTTCCGTTTATGCCACAGAAGGGTAAAAGGGACTTCCAGCTTTTTTTGCTGGCTTTCATCaa < 1:1228365/66‑1 (MQ=255) tacggCTGTTCCGTTTATGCCACAGAAGGGTAAAAGGGACTTCCAGCTTTTTTTGCTGGCTTTCATCaa < 1:948519/66‑1 (MQ=255) tacggCTGTTCCGTTTATGCCACAGAAGGGTAAAAGGGACTTCCAGCTTTTTTTGCTGGCTTTCATCaa < 1:2640301/66‑1 (MQ=255) tacggCTGTTCCGTTTATGCCACAGAAGGGTAAAAGGGACTTCCAGCTTTTTTTGCTGGCTTTCATCaa < 1:697212/66‑1 (MQ=255) acggCTGTTCCGTTTATGCCACAGAAGGGTAAAAGGGACTTCCAGCTTTTTTTGCTGGCTTTCATCaa < 1:704574/66‑1 (MQ=255) ccGTTTATGCCACAGCAGGGTAAAAGGAACTTCCAGCTTTTTTTGCTGGCTTTCGTCa > 1:1475232/1‑58 (MQ=255) ccGTTTATGCCACAGCAGGGTAAAAGGAACTTCCAGCTTTTTTTGCTGGCTTTCGTCa > 1:522805/1‑58 (MQ=255) ccGTTTATGCCACAGCAGGGTAAAAGGAACTTCCAGCTTTTTTTGCTGGCTTTCGTCa > 1:3142864/1‑58 (MQ=255) ccGTTTATGCCACAGCAGGGTAAAAGGAACTTCCAGCTTTTTTTGCTGGCTTTCGTCa > 1:911002/1‑58 (MQ=255) ccGTTTATGCCACAGCAGGGTAAAAGGAACTTCCAGCTTTTTTTGCTGGCTTTCGTCAAACGGgaga > 1:984685/1‑67 (MQ=255) ccGTTTATGCCACAGCAGGGTAAAAGGAACTTCCAGCTTTTTTTGCTGGCTTTCGTCAAACGGgaga > 1:1891406/1‑67 (MQ=255) ccGTTTATGCCACAGCAGGGTAAAAGGAACTTCCAGCTTTTTTTGCTGGCTTTCGTCAAACGGgaga > 1:3039990/1‑67 (MQ=255) ccGTTTATGCCACAGCAGGGTAAAAGGAACTTCCAGCTTTTTTTGCTGGCTTTCGTCAAACGGgaga > 1:1122028/1‑67 (MQ=255) ccGTTTATGCCACAGCAGGGTAAAAGGAACTTCCAGCTTTTTTTGCTGGCTTTCGTCAAACGGgaga > 1:2495395/1‑67 (MQ=255) tATGCCACAGCAGGGTAAAAGGAACTTCCAGCTTTTTTTGCTGGCTTTCGTCAAACGGGAGAGGAaga > 1:810857/1‑68 (MQ=255) tATGCCACAGCAGGGTAAAAGGAACTTCCAGCTTTTTTTGCTGGCTTTCGTCAAACGGGAGAGGAaga > 1:1540132/1‑68 (MQ=255) tATGCCACAGCAGGGTAAAAGGAACTTCCAGCTTTTTTTGCTGGCTTTCGTCAAACGGGAGAGGAaga > 1:917385/1‑68 (MQ=255) tATGCCACAGCAGGGTAAAAGGAACTTCCAGCTTTTTTTGCTGGCTTTCGTCAAACGGGAGAGGAaga > 1:2240524/1‑68 (MQ=255) | TAAAGGTTTTTAATGGTTTCCCGTAACCAGACGATCTTCGGATTATGGCTGTTCCGTTTATGCCACAGCAGGGTAAAAGGAACTTCCAGCTTTTTTTGCTGGCTTTCGTCAAACGGGAGAGGAAGA > minE/2480770‑2480895 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |