| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,481,036 | T→C | 37.1% | G226G (GGA→GGG) | yidZ ← | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,481,036 | 0 | T | C | 37.1% | 60.4 / 67.2 | 105 | G226G (GGA→GGG) | yidZ | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (49/17); new base C (27/12); total (76/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.53e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01 | |||||||||||
Only 100 of 105 total aligned reads displayed.
GCGCTGCCATAAACAGTGACTGCTCGAATTCCGGCAGGCTCATAGCAATCGTGCGTTCGCGTCCCAGCTCCTGTAACACATTGTCCAGCGCCCAGGTATCGCTCTGTTCCCAGCAAATGCTGATA > minE/2480975‑2481099 | gcgcTGCCATAAACAGTGACTGCTCGAATTCCGGCAGGCTCATAGCAATCGTGCGTTCGCGTCCCAGCt > 1:1905770/1‑69 (MQ=255)gcgcTGCCATAAACAGTGACTGCTCGAATTCCGGCAGGCTCATAGCAATCGTGCGTTCGCGTCCCAGCt > 1:78768/1‑69 (MQ=255)gcgcTGCCATAAACAGTGACTGCTCGAATTCCGGCAGGCTCATAGCAATCGTGCGTTCGCGTCCCAGCt > 1:410789/1‑69 (MQ=255) aaCAGTGACTGCTCGAATTCCGGCAGGCTCATAGCAATCGTGCGTTCGCGTCCCAGCTCCTGTAacac > 1:2587837/1‑68 (MQ=255) aaCAGTGACTGCTCGAATTCCGGCAGGCTCATAGCAATCGTGCGTTCGCGTCCCAGCTCCTGTAAcaca > 1:1747483/1‑69 (MQ=255) aaCAGTGACTGCTCGAATTCCGGCAGGCTCATAGCAATCGTGCGTTCGCGTCCCAGCTCCTGTAAcaca > 1:1071428/1‑69 (MQ=255) aaCAGTGACTGCTCGAATTCCGGCAGGCTCATAGCAATCGTGCGTTCGCGTCCCAGCTCCTGTAAcaca > 1:3091580/1‑69 (MQ=255) aaCAGTGACTGCTCGAATTCCGGCAGGCTCATAGCAATCGTGCGTTCGCGTCCCAGCTCCTGTAAcaca > 1:2757469/1‑69 (MQ=255) aCAGTGACTGCTCGAATTCCGGCAGGCTCATAGCAATCGTGCGTTCGCGTCCCAg > 1:1815620/1‑55 (MQ=255) aCAGTGACTGCTCGAATTCCGGCAGGCTCATAGCAATCGTGCGTTCGCGTCCCAGCTCCTGTAACACAt > 1:1036701/1‑69 (MQ=255) aCAGTGACTGCTCGAATTCCGGCAGGCTCATAGCAATCGTGCGTTCGCGTCCCAGCTCCTGTAACACAt > 1:1957517/1‑69 (MQ=255) aCAGTGACTGCTCGAATTCCGGCAGGCTCATAGCAATCGTGCGTTCGCGTCCCAGCTCCTGTAACACAt > 1:2114144/1‑69 (MQ=255) tGACTGCTCGAATTCCGGCAGGCTCATAGCAATCGTGCGTTCGCGTCCCAGCTCCTGTAAGACATTg > 1:1595984/1‑67 (MQ=255) tGACTGCTCGAATTCCGGCAGGCTCATAGCAATCGTGCGTTCGCGTCCCAGCTCCTGTAACACATTg > 1:2647353/1‑67 (MQ=255) tGACTGCTCGAATTCCGGCAGGCTCATAGCAATCGTGCGTTCGCGTCCCAGCTCCTGTAACACATTg > 1:2912005/1‑67 (MQ=255) tGACTGCTCGAATTCCGGCAGGCTCATAGCAATCGTGCGTTCGCGTCCCAGCTCCTGTAACACATTg > 1:3405807/1‑67 (MQ=255) tGACTGCTCGAATTCCGGCAGGCTCATAGCAATCGTGCGTTCGCGTCCCAGCTCCTGTAACACATTg > 1:1925574/1‑67 (MQ=255) tGACTGCTCGAATTCCGGCAGGCTCATAGCAATCGTGCGTTCGCGTCCCAGCTCCTGTAACACATTg > 1:3168631/1‑67 (MQ=255) tGACTGCTCGAATTCCGGCAGGCTCATAGCAATCGTGCGTTCGCGTCCCAGCTCCTGTAACACATTg > 1:3341007/1‑67 (MQ=255) tGACTGCTCGAATTCCGGCAGGCTCATAGCAATCGTGCGTTCGCGTCCCAGCTCCTGTAACACATTg > 1:3238652/1‑67 (MQ=255) tGACTGCTCGAATTCCGGCAGGCTCATAGCAATCGTGCGTTCGCGTCCCAGCTCCTGTAACACATTg > 1:812782/1‑67 (MQ=255) tGACTGCTCGAATTCCGGCAGGCTCATAGCAATCGTGCGTTCGAGTCCCAGCTCCTGTAaca > 1:792660/1‑62 (MQ=255) gACTGCTCGAATTCCGGCAGGCTCATAGCAATCGTGCGTTCGCGTCCCAGCTCCTGTAACACATTGTcc > 1:248867/1‑69 (MQ=255) tGCTCGAATTCCGGCAGGCTCATAGCAATCGTGCGTTCGCGTcc > 1:271591/1‑44 (MQ=255) tGCTCGAATTCCGGCAGGCTCATAGCAATCGTGCGTTCGCGTCCCAGCTCCTGTAACACATTGTCCAgc > 1:2763031/1‑69 (MQ=255) tGCTCGAATTCCGGCAGGCTCATAGCAATCGTGCGTTCGCGTCCCAGCTCCTGTAACACATTGTCCAgc > 1:1213096/1‑69 (MQ=255) tGCTCGAATTCCGGCAGGCTCATAGCAATCGTGCGTTCGCGTCCCAGCTCCTGTAACACATTGTCCAgc > 1:2888395/1‑69 (MQ=255) gCTCGAATTCCGGCAGGCTCATAGCAATCGTGCGTTCGCGTCCCAGCTCCTGTAACACATTGTCCAgcg > 1:184976/1‑69 (MQ=255) gCTCGAATTCCGGCAGGCTCATAGCAATCGTGCGTTCGCGTCCCAGCTCCTGTAACACATTGTCCAgcg > 1:2687096/1‑69 (MQ=255) gCTCGAATTCCGGCAGGCTCATAGCAATCGTGCGTTCGCGTCCCAGCTCCTGTAACACATTGTCCAgcg > 1:3059090/1‑69 (MQ=255) gCTCGAATTCCGGCAGGCTCATAGCAATCGTGCGTTCGCGTCCCAGCTCCTGTAACACATTGTCCAgcg > 1:259789/1‑69 (MQ=255) gCTCGAATTCCGGCAGGCTCATAGCAATCGTGCGTTCGCGTCCCAGCTCCTGTAACACATTGTCCAgcg > 1:418536/1‑69 (MQ=255) ccGGCAGGCTCATAGCAATCGTGCGTTCGCGCCCCAGCTCCt > 1:2102574/1‑42 (MQ=255) ccGGCAGGCTCATAGCAATCGTGCGTTCGCGCCCCAGCTCCt > 1:1241895/1‑42 (MQ=255) ccGGCAGGCTCATAGCAATCGTGCGTTCGCGCCCCAGCTCCt > 1:428200/1‑42 (MQ=255) ccGGCAGGCTCATAGCAATCGTGCGTTCGCGCCCCAGCTCCt > 1:3131427/1‑42 (MQ=255) ccGGCAGGCTCATAGCAATCGTGCGTTCGCGCCCCAGCTCCt > 1:1529292/1‑42 (MQ=255) ccGGCAGGCTCATAGCAATCGTGCGTTCGCGCCCCAGCTCCt > 1:3386055/1‑42 (MQ=255) ccGGCAGGCTCATAGCAATCGTGCGTTCGCGCCCCAGCTCCt > 1:1486580/1‑42 (MQ=255) ccGGCAGGCTCATAGCAATCGTGCGTTCCCGCCCCAGCTCCt > 1:3043383/1‑42 (MQ=38) ggCTCATAGCAATCGTGCGTTCGCGTCCCAGCTCCTGTAACACATTGTCCAGCGCCCAGGTATCGctct < 1:205566/69‑1 (MQ=255) tCATAGCAATCGTGCGTTCGCGTCCCAGCTCCTGTAAcaca < 1:2503219/41‑1 (MQ=255) tCATAGCAATCGTGCGTTCGCGTCCCAGCTCCTGTAAcaca < 1:500862/41‑1 (MQ=255) tCATAGCAATCGTGCGTTCGCGTCCCAGCTCCTGTAAcaca < 1:2975064/41‑1 (MQ=255) tCATAGCAATCGTGCGTTCGCGTCCCAGCTCCTGTAAcaca < 1:2831456/41‑1 (MQ=255) tCATAGCAATCGTGCGTTCGCGTCCCAGCTCCTGTAAcaca < 1:1883560/41‑1 (MQ=255) tCATAGCAATCGTGCGTTCGCGTCCCAGCTCCTGTAAcaca < 1:1606258/41‑1 (MQ=255) tCATAGCAATCGTGCGTTCGCGTCCCAGCTCCTGTAAcaca < 1:114333/41‑1 (MQ=255) tCATAGCAATCGTGCGTTCGCGTCCCAGCTCCTGTAAcaca < 1:2895314/41‑1 (MQ=255) tCATAGCAATCGTGCGTTCGCGCCCCAGCTCCTGTAACACATTGTCCAGCGCCCAGGTATCGCTCTGtt < 1:1181195/69‑1 (MQ=255) tCATAGCAATCGTGCGTTCGCGCCCCAGCTCCTGTAACACATTGTCCAGCGCCCAGGTATCGCTCTGtt < 1:402854/69‑1 (MQ=255) tCATAGCAATCGTGCGTTCGCGCCCCAGCTCCTGTAACACATTGTCCAGCGCCCAGGTATCGCTCTGtt < 1:2809279/69‑1 (MQ=255) tCATAGCAATCGTGCGTTCGCGCCCCAGCTCCTGTAACACATTGTCCAGCGCCCAGGTATCGCTCTGtt < 1:1000476/69‑1 (MQ=255) tCATAGCAATCGTGCGTTCGCGCCCCAGCTCCTGTAACACATTGTCCAGCGCCCAGGTATCGCTCTGtt < 1:3120369/69‑1 (MQ=255) tCATAGCAATCGTGCGTTCGCGCCCCAGCTCCTGTAACACATTGTCCAGCGCCCAGGTATCGCTCTGtt < 1:2646229/69‑1 (MQ=255) tCATAGCAATCGTGCGTTCGCGCCCCAGCTCCTGTAACACATTGTCCAGCGCCCAGGTATCGCTCTGtt < 1:1627600/69‑1 (MQ=255) tCATAGCAATCGTGCGTTCGCGCCCCAGCTCCTGTAACACATTGTCCAGCGCCCAGGTATCGCTCTGtt < 1:1445625/69‑1 (MQ=255) tCATAGCAATCGTGCGTTCGCGCCCCAGCTCCTGTAACACATTGTCCAGCGCCCAGGTATCGCTCTGtt < 1:1802260/69‑1 (MQ=255) tCATAGCAATCGTGCGTTCGCGCCCCAGCTCCTGTAACACATTGTCCAGCGCCCAGGTATCGCTCTGtt < 1:751727/69‑1 (MQ=255) tCATAGCAATCGTGCGTTCGCGCCCCAGCTCCTGTAACACATTGTCCAGCGCCCAGGTATCGCTCTGtt < 1:1065188/69‑1 (MQ=255) gCAATCGTGCGTTCGCGTCCCAGCTCCTGTAACACATTGTCCAGCGCCCAGGTATCGCTCTGTTCCCa > 1:1936012/1‑68 (MQ=255) gCAATCGTGCGTTCGCGTCCCAGCTCCTGTAACACATTGTCCAGCGCCCAGGTATCGCTCTGTTCCCa > 1:2044415/1‑68 (MQ=255) aaTCGTGCGTTCGCGTCCCAGCTCCTGTAACACATTGTCCAGCGCCCAGGTATCGCTCTGTTCCCAGCa < 1:1979521/69‑1 (MQ=255) aaTCGTGCGTTCGCGTCCCAGCTCCTGTAACACATTGTCCAGCGCCCAGGTATCGCTCTGTTCCCAGCa < 1:2797262/69‑1 (MQ=255) aaTCGTGCGTTCGCGTCCCAGCTCCTGTAACACATTGTCCAGCGCCCAGGTATCGCTCTGTTCCCAGCa < 1:2106911/69‑1 (MQ=255) aaTCGTGCGTTCGCGTCCCAGCTCCTGTAACACATTGTCCAGCGCCCAGGTATCGCTCTGTTCCCAGCa < 1:1904304/69‑1 (MQ=255) aaTCGTGCGTTCGCGTCCCAGCTCCTGTAACACATTGTCCAGCGCCCAGGTATCGCTCTGTTCCCAGCa < 1:2814532/69‑1 (MQ=255) aaTCGTGCGTTCGCGTCCCAGCTCCTGTAACACATTGTCCAGAGCCCAGGTATCGCTCTGTTCCCAGCa < 1:876743/69‑1 (MQ=255) aTCGTGCGTTCGCGTCCCAGCTCCTGTAACACATTGTCCAGCGCCCAGGTATCGCTCTGTTCCCAGCaa > 1:584880/1‑69 (MQ=255) cGTGCGTTCGCGTCCCAGCTCCTGTAACACATTGTCCAGCGCCCAGGTATCGCTCTGTTCCCAGCaaa < 1:600852/68‑1 (MQ=255) gTGCGTTCGCGTCCCAGCTCCTGTAGCACATTGTCCAGGGCCCAGGTATCGCTCTGTTCCCAGCAAATg > 1:3424812/1‑69 (MQ=255) gTGCGTTCGCGTCCCAGCTCCTGTAACACATTGTCCAGCGCCCAGGTATCGCTCTGTTCCCAGCAAATg > 1:879228/1‑69 (MQ=255) gTGCGTTCGCGTCCCAGCTCCTGTAACACATTGTCCAGCGCCCAGGTATCGCTCTGTTCCCAGCAAATg > 1:598310/1‑69 (MQ=255) gTGCGTTCGCGTCCCAGCTCCTGTAACACATTGTCCAGCGCCCAGGTATCGCTCTGTTCCCAGCAAATg > 1:675529/1‑69 (MQ=255) gTGCGTTCGCGTCCCAGCTCCTGTAACACATTGTCCAGCGCCCAGGTATCGCTCTGTTCCCAGCAAATg > 1:3204362/1‑69 (MQ=255) gTGCGTTCGCGTCCCAGCTCCTGTAACACATTGTCCAGCGCCCAGGTATCGCTCTGTTCCCAGCAAATg > 1:317311/1‑69 (MQ=255) gTGCGTTCGCGTCCCAGCTCCTGTAACACATTGTCCAGCGCCCAGGTATCGCTCTGTTCCCAGCAAATg > 1:1765640/1‑69 (MQ=255) gTGCGTTCGCGTCCCAGCTCCTGTAACACATTGTCCAGCGCCCAGGTATCGCTCTGTTCCCAGCAAATg > 1:2997425/1‑69 (MQ=255) gTGCGTTCGCGTCCCAGCTCCTGTAACACATTGTCCAGCGCCCAGGTATCGCTCTGTTCCCAGCAAATg > 1:1928806/1‑69 (MQ=255) gTGCGTTCGCGTCCCAGCTCCTGTAACACATTGTCCAGCGCCCAGGTATCGCTCTGTTCCCAGCAAATg > 1:3215960/1‑69 (MQ=255) gTGCGTTCGCGTCCCAGCTCCTGTAACACATTGTCCAGCGCCCAGGTATCGCTCTGTTCCCAGCAAATg > 1:2346092/1‑69 (MQ=255) gTGCGTTCGCGTCCCAGCTCCTGTAACACATTGTCCAGCGCCCAGGTATCGCTCTGTTCCCAGCAAATg > 1:2509491/1‑69 (MQ=255) tCGCGCCCCAGCTCCTGTAACACATTGTCCAGCGCCCAGGTTTCGCTCTGTTCCCAGCAAATGCTGat > 1:3327635/1‑68 (MQ=255) tCGCGCCCCAGCTCCTGTAACACATTGTCCAGCGCCCAGGTATTGCTCTGTTCCCAGCAAATGCTGata > 1:561697/1‑69 (MQ=255) tCGCGCCCCAGCTCCTGTAACACATTGTCCAGCGCCCAGGTATCGCTCTGTTCCCAGCAAATGCTGata > 1:2516425/1‑69 (MQ=255) tCGCGCCCCAGCTCCTGTAACACATTGTCCAGCGCCCAGGTATCGCTCTGTTCCCAGCAAATGCTGata > 1:2713420/1‑69 (MQ=255) tCGCGCCCCAGCTCCTGTAACACATTGTCCAGCGCCCAGGTATCGCTCTGTTCCCAGCAAATGCTGata > 1:2820808/1‑69 (MQ=255) tCGCGCCCCAGCTCCTGTAACACATTGTCCAGCGCCCAGGTATCGCTCTGTTCCCAGCAAATGCTGata > 1:343263/1‑69 (MQ=255) tCGCGCCCCAGCTCCTGTAACACATTGTCCAGCGCCCAGGTATCGCTCTGTTCCCAGCAAATGCTGata > 1:584367/1‑69 (MQ=255) tCGCGCCCCAGCTCCTGTAACACATTGTCCAGCGCCCAGGTATCGCTCTGTTCCCAGCAAATGCTGata > 1:197331/1‑69 (MQ=255) tCGCGCCCCAGCTCCTGTAACACATTGTCCAGCGCCCAGGTATCGCTCTGTTCCCAGCAAATGCTGata > 1:2824449/1‑69 (MQ=255) tCGCGCCCCAGCTCCTGTAACACATTGTCCAGCGCCCAGGTATCGCTCTGTTCCCAGCAAATGCTGata > 1:614418/1‑69 (MQ=255) tCGCGCCCCAGCTCCTGTAACACATTGTCCAGCGCCCAGGTATCGCTCTGTTCCCAGCAAATGCTGata > 1:3007384/1‑69 (MQ=255) tCGCGCCCCAGCTCCTGTAACACATTGTCCAGCGCCCAGGTATCGCTCTGTTCCCAGCAAATGCTGata > 1:1476039/1‑69 (MQ=255) tCGCGCCCCAGCTCCTGTAACACATTGTCCAGCGCCCAGGTATCGCTCTGTTCCCAGCAAATGCTGata > 1:1390745/1‑69 (MQ=255) tCGCGCCCCAGCTCCTGTAACACATTGTCCAGCGCCCAGGTATCGCTCTGTTCCCAGCAAATGCTGata > 1:1386899/1‑69 (MQ=255) tCGCGCCCCAGCTCCTGTAACACATTGTCCAGCGCCCAGGTATCGCTCTGTTCCCAGCAAATGCTGata > 1:1160511/1‑69 (MQ=255) tCGCGCCCCAGCTCCTGTAACACATTGTCCAGCGCCCAGGTATCGCTCTGTTCCCAGCAAATGCTGata > 1:1094945/1‑69 (MQ=255) tCGCGCCCCAGCTCCTGTAACACATTGTCCAGCGCCCAGGTATCGCTCTGTTCCCAGCAAATGCTGat > 1:1770376/1‑68 (MQ=255) tCGCGCCCCAGCTCCTGTAACACATTGTCCAGCGCCCAGGTATCGCTCTGTTCCCAGCAAATGCTGat > 1:1592201/1‑68 (MQ=255) | GCGCTGCCATAAACAGTGACTGCTCGAATTCCGGCAGGCTCATAGCAATCGTGCGTTCGCGTCCCAGCTCCTGTAACACATTGTCCAGCGCCCAGGTATCGCTCTGTTCCCAGCAAATGCTGATA > minE/2480975‑2481099 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |