| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,482,042 | C→T | 31.5% | S274S (TCG→TCA) | mdtL ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,482,042 | 0 | C | T | 31.5% | 90.2 / 47.5 | 98 | S274S (TCG→TCA) | mdtL | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base C (48/19); new base T (12/19); total (60/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.37e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01 | |||||||||||
AGAAACCGCATGGGAAGGTGAAACGGCAAGAGTGATCCCCGCCGCCAGGAATAACACCTGCGAGGTGATCATCAACGTACGTGGCTTAAAAATTCCCAGCGCAAATGGCGTGGAGAATGAAACGGTC > minE/2481982‑2482108 | agaAACCGCATGGGAAGGTGAAACGGCAAGAGTGATCCCCGCCGCCAGGAATAACACCTGCGAGGTGat > 1:9460/1‑69 (MQ=255)agaAACCGCATGGGAAGGTGAAACGGCAAGAGTGATCCCCGCCGCCAGGAATAACACCTGCGAGGTGat > 1:2114005/1‑69 (MQ=255)agaAACCGCATGGGAAGGTGAAACGGCAAGAGTGATCCCCGCCGCCAGGAATAACACCTGCGAGGTGat > 1:2999288/1‑69 (MQ=255)agaAACCGCATGGGAAGGTGAAACGGCAAGAGTGATCCCCGCCGCCAGGAATAACACCTGCGAGGTGat > 1:394961/1‑69 (MQ=255)agaAACCGCATGGGAAGGTGAAACGGCAAGAGTGATCCCCGCCGCCAGGAATAACACCTGCGAGGTGat > 1:1306544/1‑69 (MQ=255)agaAACCGCATGGGAAGGTGAAACGGCAAGAGTGATCCCCGCCGCCAGGAATAACACCTGCGAGGTGat > 1:1278551/1‑69 (MQ=255)agaAACCGCATGGGAAGGTGAAACGGCAAGAGTGATCCCCGCCGCCAGGAATAACACCTGCGAGGTGat > 1:731080/1‑69 (MQ=255)agaAACCGCATGGGAAGGTGAAACGGCAAGAGTGATCCCCGCCGCCAGGAATAACACCTGCGAGGTGat > 1:859670/1‑69 (MQ=255)agaAACCGCATGGGAAGGTGAAACGGCAAGAGTGATCCCCGCCGCCAGGAATAACACCTGCGAGGTGat > 1:1072154/1‑69 (MQ=255)agaAACCGCATGGGAAGGTGAAACGGCAAGAGTGATCCCCGCCGCCAGGAATAACACCTGCGAGGTGa > 1:308374/1‑68 (MQ=255) gaAACCGCATGGGAAGGTGAAACGGCAAGAGTGATCCCCGCCGCCAGGAATAACACCTGCGAGGTGatc < 1:2614429/69‑1 (MQ=255) gaAACCGCATGGGAAGGTGAAACGGCAAGAGTGATCCCCGCCGCCAGGAATAACACCTGCGAGGTGatc < 1:3434273/69‑1 (MQ=255) aCCGCATGGGAAGGTGAAACGGCAAGAGTGATCCCCGCCGCCAGGAATAACACCTGCGAGGTGatcatc > 1:2496813/1‑69 (MQ=255) aCCGCATGGGAAGGTGAAACGGCAAGAGTGATCCCCGCCGCCAGGAATAACACCTGCGAGGTGatcatc > 1:564543/1‑69 (MQ=255) aCCGCATGGGAAGGTGAAACGGCAAGAGTGATCCCCGCCGCCAGGAATAACACCTGCGAGGTGatcatc > 1:48690/1‑69 (MQ=255) aCCGCATGGGAAGGTGAAACGGCAAGAGTGATCCCCGCCGCCAGGAATAACACCTGCGAGGTGatcatc > 1:381238/1‑69 (MQ=255) cATGGGAAGGTGAAACGGCAAGAGTGATCCCCGCCGCCAGGAATAACACCTGCGAGGTGATCATCAacg < 1:1401925/69‑1 (MQ=255) cATGGGAAGGTGAAACGGCAAGAGTGATCCCCGCCGCCAGGAATAACACCTGCGAGGTGATCATCAacg < 1:1882033/69‑1 (MQ=255) cATGGGAAGGTGAAACGGCAAGAGTGATCCCCGCCGCCAGGAATAACACCTGCGAGGTGATCATCAacg < 1:748532/69‑1 (MQ=255) tGGGAAGGTGAAACGGCAAGAGTGATCCCCGCCGCCAGGAATAACACCTGCGAGGTGATCATCAacgta < 1:1286640/69‑1 (MQ=255) gggAAGGTGAAACGGCAAGAGTGATCCCCGCCGCCAGGAATAACACCTGCGAGGTGATCATCAacgta < 1:1461019/68‑1 (MQ=255) gggAAGGTGAAACGGCAAGAGTGATCCCCGCCGCCAGGAATAACACCTGCGAGGTGATCATCAacgta < 1:1205902/68‑1 (MQ=255) gggAAGGTGAAACGGCAAGAGTGATCCCCGCCGCCAGGAATAACACCTGCGAGGTGATCATCAacgta < 1:722509/68‑1 (MQ=255) ggAAGGTGAAACGGCAAGAGTGATCCCCGCCGCCAGGAATAACACCTGCGAGGTGatca > 1:2425619/1‑59 (MQ=255) ggAAGGTGAAACGGCAAGAGTGATCCCCGCCGCCAGGAATAACACCTGCGAGGTGATCATCAacgtacg > 1:110173/1‑69 (MQ=255) ggAAGGTGAAACGGCAAGAGTGATCCCCGCCGCCAGGAATAACACCTGCGAGGTGATCATCAacgtac > 1:3077292/1‑68 (MQ=255) aaGGTGAAACGGCAAGGGTGATCCCCGCCGCCAGGAATAACACCTGTGAGGTGATCATCAACGTACGTg > 1:979822/1‑69 (MQ=255) aaGGTGAAACGGCAAGGGTGATCCCCGCCGCCAGGAATAACACCTGTGAGGTGATCATCAACGTACGTg > 1:2458419/1‑69 (MQ=255) aaGGTGAAACGGCAAGGGTGATCCCCGCCGCCAGGAATAACACCTGTGAGGTGATCATCAACGTACGTg > 1:2174230/1‑69 (MQ=255) aaGGTGAAACGGCAAGGGTGATCCCCGCCGCCAGGAATAACACCTGTGAGGTGATCATCAACGTACGTg > 1:2687913/1‑69 (MQ=255) aaGGTGAAACGGCAAGGGTGATCCCCGCCGCCAGGAATAACACCTGTGAGGTGATCATCAACGTACGTg > 1:2688990/1‑69 (MQ=255) aaGGTGAAACGGCAAGGGTGATCCCCGCCGCCAGGAATAACACCTGTGAGGTGATCATCAACGTACGTg > 1:2833445/1‑69 (MQ=255) aaGGTGAAACGGCAAGGGTGATCCCCGCCGCCAGGAATAACACCTGTGAGGTGATCATCAACGTACGTg > 1:2888367/1‑69 (MQ=255) aaGGTGAAACGGCAAGGGTGATCCCCGCCGCCAGGAATAACACCTGTGAGGTGATCATCAACGTACGTg > 1:329237/1‑69 (MQ=255) aaGGTGAAACGGCAAGGGTGATCCCCGCCGCCAGGAATAACACCTGTGAGGTGATCATCAACGTACGTg > 1:648878/1‑69 (MQ=255) aaGGTGAAACGGCAAGGGTGATCCCCGCCGCCAGGAATAACACCTGTGAGGTGATCATCAACGTACGTg > 1:720824/1‑69 (MQ=255) aaGGTGAAACGGCAAGGGTGATCCCCGCCGCCAGGAATAACACCTGTGAGGTGATCATCAACGTACGTg > 1:2076200/1‑69 (MQ=255) aaGGTGAAACGGAAAGGGTGATCCCCGCCGCCAGGAATAACACCTGTGAGGTGATCATCAACGTACGTg > 1:2729063/1‑69 (MQ=255) aGGTGAAACGGCAAGGGTGATCCCCGCCTCCAGGAATAACACCTGTGAGGTGat < 1:1351615/54‑1 (MQ=255) aGGTGAAACGGCAAGGGTGATCCCCGCCGCCAGGAATAACACCTGTGAGGTGat < 1:1060977/54‑1 (MQ=255) aGGTGAAACGGCAAGGGTGATCCCCGCCGCCAGGAATAACACCTGTGAGGTGat < 1:2685738/54‑1 (MQ=255) aGGTGAAACGGCAAGGGTGATCCCCGCCGCCAGGAATAACACCTGTGAGGTGat < 1:2763644/54‑1 (MQ=255) aGGTGAAACGGCAAGGGTGATCCCCGCCGCCAGGAATAACACCTGTGAGGTGat < 1:2978668/54‑1 (MQ=255) aGGTGAAACGGCAAGGGTGATCCCCGCCGCCAGGAATAACACCTGTGAGGTGat < 1:1542561/54‑1 (MQ=255) aGGTGAAACGGCAAGGGTGATCCCCGCCGCCAGGAATAACACCTGTGAGGTGat < 1:3090771/54‑1 (MQ=255) aGGTGAAACGGCAAGGGTGATCCCCGCCGCCAGGAATAACACCTGTGAGGTGat < 1:1525164/54‑1 (MQ=255) aGGTGAAACGGCAAGGGTGATCCCCGCCGCCAGGAATAACACCTGTGAGGTGat < 1:1830842/54‑1 (MQ=255) aGGTGAAACGGCAAGGGTGATCCCCGCCGCCAGGAATAACACCTGTGAGGTGat < 1:3428771/54‑1 (MQ=255) aGGTGAAACGGCAAGGGTGATCCCCGCCGCCAGGAATAACACCTGTGAGGTGat < 1:1456763/54‑1 (MQ=255) aGGTGAAACGGCAAGGGTGATCCCCGCCGCCAGGAATAACACCTGTGAGGTGat < 1:203581/54‑1 (MQ=255) aGGTGAAACGGCAAGGGTGATCCCCGCCGCCAGGAATAACACCTGTGAGGTGat < 1:1328397/54‑1 (MQ=255) aGGTGAAACGGCAAGGGTGATCCCCGCCGCCAGGAATAACACCTGTGAGGTGat < 1:119141/54‑1 (MQ=255) aGGTGAAACGGCAAGGGTGATCCCCGCCGCCAGGAATAACACCTGTGAGGTGat < 1:1080847/54‑1 (MQ=255) aGGTGAAACGGCAAGGGTGATCCCCGCCGCCAGGAATAACACCTGTGAGGTGat < 1:782103/54‑1 (MQ=255) aGGTGAAACGGCAAGGGTGATCCCCGCCGCCAGGAATAACACCTGTGAGGTGat < 1:959204/54‑1 (MQ=255) aGGTGAAACGGCAAGGGTGATCCCCGCCACCAGGAATAACACCTGTGAGGTGat < 1:1035185/54‑1 (MQ=255) aGGGGAAACGGCAAGGCTGATCCCCGCCGCCAGGAATAACACCTGTGAGGGGat < 1:2684112/54‑1 (MQ=25) gTGAAACGGCAAGAGTGATCCCCGCCGCCAGGAATAACACCTGCGAGGTGATCATCAACGTACGTGGc > 1:1144929/1‑68 (MQ=255) gTGAAACGGCAAGAGTGATCCCCGCCGCCAGGAATAACACCTGCGAGGTGATCATCAACGTACGTGGc > 1:1778963/1‑68 (MQ=255) gTGAAACGGCAAGAGTGATCCCCGCCGCCAGGAATAACACCTGCGAGGTGATCATCAACGTACGTGGc > 1:1545318/1‑68 (MQ=255) aCGGCAAGAGTGATCCCCGCCGCCAGGAATAACACCTGCGAGGTTATCATCAACGTACGTGGCTTaaa < 1:3321248/68‑1 (MQ=255) aCGGCAAGAGTGATCCCCGCCGCCAGGAATAACACCTGCGAGGTGATCATCAACGTACGTGGCTTaaa < 1:3374453/68‑1 (MQ=255) aCGGCAAGAGTGATCCCCGCCGCCAGGAATAACACCTGCGAGGTGATCATCAACGTACGTGGCTTaaa < 1:1714474/68‑1 (MQ=255) aCGGCAAGAGTGATCCCCGCCGCCAGGAATAACACCTGCGAGGTGATCATCAACGTACGTGGCTTaaa < 1:3274504/68‑1 (MQ=255) aCGGCAAGAGTGATCCCCGCCGCCAGGAATAACACCTGCGAGGTGATCATCAACGTACGTGGCTTaaa < 1:412428/68‑1 (MQ=255) aCGGCAAGAGTGATCCCCGCCGCCAGGAATAACACCTGCGAGGTGATCATCAACGTACGTGGCTTaaa < 1:477424/68‑1 (MQ=255) aCGGCAAGAGTGATCCCCGCCGCCAGGAATAACACCTGCGAGGTGATCATCAACGTACGTGGCTTaaa < 1:1608461/68‑1 (MQ=255) aCGGCAAGAGTGATCCCCGCCGCCAGGAATAACACCTGCGAGGTGATCATCAACGTACGTGGCTTaaa < 1:12370/68‑1 (MQ=255) aCGGCAAGAGTGATCCCCGCCGCCAGGAATAACACCTGCGAGGTGATCATCAACGTACGTGGCTTaaa < 1:1200600/68‑1 (MQ=255) tGATCCCCGCCGCCAGGAATAACACCTGCGAGGTGATCATCAACGTACGTGGCTTAAAAATTCCCAgc > 1:1071076/1‑68 (MQ=255) tGATCCCCGCCGCCAGGAATAACACCTGCGAGGTGATCATCAACGTACGTGGCTTAAAAATTCCCAgc > 1:1735485/1‑68 (MQ=255) aTCCCCGCCGCCAGGAATAACACCTGCGAGGTGATCATCAacgt > 1:3337649/1‑44 (MQ=255) aTCCCCGCCGCCAGGAATAACACCTGCGAGGTGATCATCAACGTACGTGGCTTAAAAATTCCCAGCGCa > 1:2546910/1‑69 (MQ=255) aTCCCCGCCGCCAGGAATAACACCTGCGAGGTGATCATCAACGTACGTGGCTTAAAAATTCCCAGCGCa > 1:2391426/1‑69 (MQ=255) aTCCCCGCCGCCAGGAATAACACCTGCGAGGTGATCATCAACGTACGTGGCTTAAAAATTCCCAGCGCa > 1:2393539/1‑69 (MQ=255) ccccGCCGCCAGGAATAACACCTGCGAGGTGATCATCAACGTACGTGGCTTAAAAATTCCCAGCGCtaa > 1:2428436/1‑69 (MQ=255) ccccGCCGCCAGGAATAACACCTGCGAGGTGATCATCAACGTACGTGGCTTAAAAATTCCCAGCGCaaa > 1:1790330/1‑69 (MQ=255) ccccGCCGCCAGGAATAACACCTGCGAGGTGATCATCAACGTACGTGGCTTAAAAATTCCCAGCGCaaa > 1:444155/1‑69 (MQ=255) gccAGGAATAACACCTGCGAGGTGATCATCAACGTACGTGGCTTAAAAATTCCCAGCGCAAATGGCGTg < 1:2738953/69‑1 (MQ=255) ggAATAACACCTGCGAGGTGATCATCAACGTACGTGGCTTAAAAATTCCCAGCGCa > 1:2131690/1‑56 (MQ=255) ggAATAACACCTGCGAGGTGATCATCAACGTACGTGGCTTAAAAATTCCCAGCGCa > 1:618780/1‑56 (MQ=255) aaTAACACCTGCGAGGTGATCATCAACGTACGTGGCTTAAAAATTCCCAGCGCAAATGGCGTGgag > 1:2868210/1‑66 (MQ=255) aaTAACACCTGCGAGGTGATCATCAACGTACGTGGCTTAAAAATTCCCAGCGCAAATGGCGTGgag > 1:2358616/1‑66 (MQ=255) aaTAACACCTGCGAGGTGATCATCAACGTACGTGGCTTAAAAATTCCCAGCGCAAATGGCGTGgag > 1:1171552/1‑66 (MQ=255) aaTAACACCTGCGAGGTGATCATCAACGTACGTGGCTTAAAAATTCCCAGCGCAAATGGCGTGgag > 1:675208/1‑66 (MQ=255) aaTAACACCTGCGAGGTGATCATCAACGTACGTGGCTTAAAAATTCCCAGCGCAAATGGCGTGgag > 1:2448838/1‑66 (MQ=255) aaTAACACCTGCGAGGTGATCATCAACGTACGTGGCTTAAAAATTCCCAGCGCAAATGGCGTGgag > 1:1620714/1‑66 (MQ=255) aaTAACACCTGCGAGGTGATCATCAACGTACGTGGCTTAAAAATTCCCAGCGCAAATGGCGTGgag > 1:2662055/1‑66 (MQ=255) aaTAACACCTGCGAGGTGATCATCAACGTACGTGGCTTAAAAATTCCCAGCGCAAATGGCGTGgag > 1:3156716/1‑66 (MQ=255) aaTAACACCTGCGAGGTGATCATCAACGTACGTGGCTTAAAAATTCCCAGCGCAAATGGCGTGgag > 1:3213797/1‑66 (MQ=255) aaTAACACCTGCGAGGTGATCATCAACGTACGTGGCTTAAAAATTCCCAGCGCAAATGGCGTGGAGaa > 1:145455/1‑68 (MQ=255) acacCTGCGAGGTGATCATCAACGTACGTGGCTTAAAAATTCCCAGCGCAAATGGCGTGGAGAATGaaa > 1:2380329/1‑69 (MQ=255) acacCTGCGAGGTGATCATCAACGTACGTGGCTTAAAAATTCCCAGCGCAAATGGCGTGGAGAATGaaa > 1:462229/1‑69 (MQ=255) acacCTGCGAGGTGATCATCAACGTACGTGGCTTAAAAATTCCCAGCGCAAATGGCGTGGAGAATGaaa > 1:1714086/1‑69 (MQ=255) acacCTGCGAGGTGATCATCAACGTACGTGGCTTAAAAATTCCCAGCGCAAATGGCGTGGAGAATGaaa > 1:3172008/1‑69 (MQ=255) acacCTGCGAGGTGATCATCAACGTACGTGGCTTAAAAATTCCCAGCGCAAATGGCGTGGAGAATGaaa > 1:2050335/1‑69 (MQ=255) acacCTGCGAGGTGATCATCAACGTACGTGGCTTAAAAATTCCCAGCGCAAATGGCGTGGAGAATGaaa > 1:2131311/1‑69 (MQ=255) tGCGAGGTGATCATCAACGTACGTGGCTTAAAAATTCCCAGCGCAAATGGCGTGGAGAATGAAACGGTc > 1:2186128/1‑69 (MQ=255) | AGAAACCGCATGGGAAGGTGAAACGGCAAGAGTGATCCCCGCCGCCAGGAATAACACCTGCGAGGTGATCATCAACGTACGTGGCTTAAAAATTCCCAGCGCAAATGGCGTGGAGAATGAAACGGTC > minE/2481982‑2482108 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |