| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,482,189 | T→C | 45.5% | S225S (TCA→TCG) | mdtL ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,482,189 | 0 | T | C | 45.5% | 15.8 / 94.0 | 112 | S225S (TCA→TCG) | mdtL | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base T (29/32); new base C (7/44); total (36/76) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.03e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 117 total aligned reads displayed.
TCAGCGCCATAATGGTGGCGTATTCACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACGTGTTGACGAAAGTGAGGATCACCGAAACGCTGAGGGTGGTGATAACAACACGGCTGAGGAAAA > minE/2482125‑2482256 | tCAGCGCCATAATGGTGGCGTATTCACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGTTGACg < 1:1329771/69‑1 (MQ=255)tCAGCGCCATAATGGTGGCGTATTCACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACg < 1:1018996/69‑1 (MQ=255)tCAGCGCCATAATGGTGGCGTATTCACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACg < 1:253401/69‑1 (MQ=255)tCAGCGCCATAATGGTGGCGTATTCACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACg < 1:1506291/69‑1 (MQ=255) cAGCGCCATAATGGTGGCGTATTCACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACg < 1:2836380/68‑1 (MQ=255) aTGGTGGCGTATTCACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACGTGTTGACGaa > 1:1975415/1‑68 (MQ=255) ggtggCGTATTCACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGt < 1:1750500/52‑1 (MQ=255) ggtggCGTATTCACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGt < 1:2404522/52‑1 (MQ=255) ggtggCGTATTCACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGt < 1:3233352/52‑1 (MQ=255) ggtggCGTATTCACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGt < 1:1557488/52‑1 (MQ=255) gtggCGTATTCACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACGTGTTGACGAAAGTg < 1:3228511/69‑1 (MQ=255) gtggCGTATTCACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACGTGTTGACGAAAGTg < 1:600268/69‑1 (MQ=255) gtggCGTATTCACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACGTGTTGACGAAAGTg < 1:748463/69‑1 (MQ=255) gtggCGTATTCACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACGTGTTGACGAAAGTg < 1:1960309/69‑1 (MQ=255) gtggCGTATTCACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACGTGTTGACGAAAGTg < 1:3190999/69‑1 (MQ=255) gtggCGTATTCACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACGTGTTGACGAAAGTg < 1:2462341/69‑1 (MQ=255) gtggCGTATTCACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACGTGTTGACGAAAGTg < 1:1915383/69‑1 (MQ=255) gtggCGTATTCACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACGTGTTGACGAAAGTg < 1:1863139/69‑1 (MQ=255) gtggCGTATTCACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACGTGTTGACGAAAGTg < 1:1690509/69‑1 (MQ=255) tggCGTATTCACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACGTGTTGACGAAAGTg < 1:862877/68‑1 (MQ=255) gCGTATTCACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACGTGTTGACGAAAGTGAgg < 1:473788/69‑1 (MQ=255) gCGTATTCACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACGTGTTGACGAAAGTGAgg < 1:1313567/69‑1 (MQ=255) gCGTATTCACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACGTGTTGACGAAAGTGAgg < 1:2437200/69‑1 (MQ=255) gCGTATTCACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACGTGTTGACGAAAGTGAgg < 1:1338316/69‑1 (MQ=255) ttCACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACGTGTTg > 1:1679970/1‑52 (MQ=255) ttCACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACGTGTTg > 1:470366/1‑52 (MQ=255) ttCACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACGTGTTg > 1:247229/1‑52 (MQ=255) ttCACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACGTGTTg > 1:3081156/1‑52 (MQ=255) ttCACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACGTGTTg > 1:146944/1‑52 (MQ=255) ttCACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACGTGTTg > 1:738956/1‑52 (MQ=255) ttCACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACGTGTTg > 1:3100424/1‑52 (MQ=255) cACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACGTGTTGACGAAAGTGAgg < 1:3086857/62‑1 (MQ=255) cACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACGTGTTGACGAAAGTGAgg < 1:2143932/62‑1 (MQ=255) cACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACGTGTTGACGAAAGTGAgg < 1:2114962/62‑1 (MQ=255) cACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACGTGTTGACGAAAGTGAgg < 1:3228614/62‑1 (MQ=255) cACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACGTGTTGACGAAAGTGAgg < 1:3388549/62‑1 (MQ=255) cACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACGTGTTGACGAAAGTGAgg < 1:949479/62‑1 (MQ=255) cACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACGTGTTGACGAAAGTGAGGATCAcc > 1:587092/1‑68 (MQ=255) ccGCGCTCAAAACCCATGATTTCCATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGa < 1:291700/68‑1 (MQ=255) ccGCGCTCAAAACCCATGATTTCCATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGa < 1:2806124/68‑1 (MQ=255) ccGCGCTCAAAACCCATGATTTCCATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGa < 1:857700/68‑1 (MQ=255) ccGCGCTCAAAACCCATGATTTCCATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGa < 1:3094456/68‑1 (MQ=255) ccGCGCTCAAAACCCATGATTTCCATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGa < 1:3382354/68‑1 (MQ=255) ccGCGCTCAAAACCCATGATTTCCATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGa < 1:1128461/68‑1 (MQ=255) ccGCGCTCAAAACCCATGATTTCCATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGa < 1:486938/68‑1 (MQ=255) ccGCGCTCAAAACCCATGATTTCCATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGa < 1:687953/68‑1 (MQ=255) ccGCGCTCAAAACCCATGATTTCCATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGa < 1:55474/68‑1 (MQ=255) ccGCGCTCAAAACCCATGATTTCCATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGa < 1:598624/68‑1 (MQ=255) ccGCGCTCAAAACCCATGATTTCCATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGa < 1:643789/68‑1 (MQ=255) ccGCGCTCAAAACCCATGATTTCCATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGa < 1:2740871/68‑1 (MQ=255) ccGCGCTCAAAACCCATGATTTCCATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGa < 1:1700754/68‑1 (MQ=255) ccGCGCTCAAAACCCATGATTTCCATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGa < 1:2378098/68‑1 (MQ=255) ccGCGCTCAAAACCCATGATTTCCATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGa < 1:1380101/68‑1 (MQ=255) ccGCGCTCAAAACCCATGATTTCCATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGa < 1:2548953/68‑1 (MQ=255) ccGCGCTCAAAACCCATGATTTCCATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGa < 1:1378847/68‑1 (MQ=255) ccGCGCTCAAAACCCATGATTTCCATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGa < 1:263152/68‑1 (MQ=255) ccGCGCTCAAAACCCATGATTTCCATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGa < 1:265459/68‑1 (MQ=255) cgcgCTCAAAACCCATGATTTCCATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGa < 1:743279/67‑1 (MQ=255) cgcgCTCAAAACCCATGATTTCCATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGa < 1:1461750/67‑1 (MQ=255) cgcgCTCAAAACCCATGATTTCCATCAGCAATACTGGCGACGTGGTGACGAAGGTGAGGATCACCGa < 1:2031473/67‑1 (MQ=255) gATTTCCATCAGCAATACCGGTGACGTGTTGACGAAAGTGAGGATCACCGAAACGCTGAGGGTGGTGAt > 1:800277/1‑69 (MQ=255) aTTTCCATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGa < 1:1198781/50‑1 (MQ=255) ttCCATCAGCAATACCGGTGACGTGTTGACGAAAGTGAGGATCACCGAAACGCTGAGGGTGGTGATaac < 1:511183/69‑1 (MQ=255) ccATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGAAACGCTGAGGGTGGTGATaacaa > 1:1350549/1‑69 (MQ=255) ccATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGAAACGCTGAGGGTGGTGATaacaa > 1:277464/1‑69 (MQ=255) ccATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGAAACGCTGAGGGTGGTGATaacaa > 1:978401/1‑69 (MQ=255) ccATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGAAACGCTGAGGGTGGTGATaacaa > 1:3421305/1‑69 (MQ=255) ccATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGAAACGCTGAGGGTGGTGATaacaa > 1:3288348/1‑69 (MQ=255) ccATCAGCAATACTGGCGAAGTGTTGACGAAGGTGAGGATCACCGaa > 1:3296321/1‑47 (MQ=255) cATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGAAACGCTGAGGGTGGTGATaacaa < 1:322371/68‑1 (MQ=255) cATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGAAACGCTGAGGGTGGTGATaacaa < 1:2336841/68‑1 (MQ=255) cATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGAAACGCTGAGGGTGGTGATaacaa < 1:1595561/68‑1 (MQ=255) cATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGAAACGCTGAGGGTGGTGATaacaa < 1:765972/68‑1 (MQ=255) cATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGAAACGCTGAGGGTGGTGATaacaa < 1:1719173/68‑1 (MQ=255) cATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGAAACGCTGAGGGTGGTGATaacaa < 1:81172/68‑1 (MQ=255) cATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGAAACGCTGAGGGTGGTGATaacaa < 1:117549/68‑1 (MQ=255) cATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGAAACGCTGAGGGTGGTGATaacaa < 1:1156413/68‑1 (MQ=255) cATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGAAACGCTGAGGGTGGTGATaacaa < 1:1919110/68‑1 (MQ=255) cATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGAAACGCTGAGGGTGGTGATaacaa < 1:3338045/68‑1 (MQ=255) cATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGAAACGCTGAGGGTGGTGATaacaa < 1:204735/68‑1 (MQ=255) cATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGAAACGCTGAGGGTGGTGATaacaa < 1:3280749/68‑1 (MQ=255) cATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGAAACGCTGAGGGTGGTGATaacaa < 1:1923694/68‑1 (MQ=255) cATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGCATCACCGAAACGCTGAGGGTGGTGATaacaa < 1:2932546/68‑1 (MQ=255) cATCAGCAATACCGGTGACGTGTTGACGAAAGTGAGGATCACCGAAACGCTGAGGGTGGTGATaacaa < 1:2849904/68‑1 (MQ=255) cATCAGCAATACCGGTGACGTGTTGACGAAAGTGAGGATCACCGAAACGCTGAGGGTGGTGATaacaa < 1:3083725/68‑1 (MQ=255) aaTACCGGTGACGTGTTGACGAAAGTGAGGATCACCGAAACGCTGAGGGTGGTGATAACAACACGGCTg > 1:3006054/1‑69 (MQ=255) aaTACCGGTGACGTGTTGACGAAAGTGAGGATCACCGAAACGCTGAGGGTGGTGATAACAACACGGCTg > 1:1270520/1‑69 (MQ=255) aaTACCGGTGACGTGTTGACGAAAGTGAGGATCACCGAAACGCTGAGGGTGGTGATAACAACACGGCTg > 1:2246377/1‑69 (MQ=255) aaTACCGGTGACGTGTTGACGAAAGTGAGGATCACCGAAACGCTGAGGGTGGTGATAACAACACGGCTg > 1:217861/1‑69 (MQ=255) aTACTGGCGACGTGTTGACGAAGGTGAGGATCACCGa < 1:1817353/37‑1 (MQ=25) ccGGTGACGTGTTGACGAAAGTGAGGATCACCGAAACGCTGAGGGTGGTGATAACAACACGGCTGAgg > 1:3095023/1‑68 (MQ=255) ccGGTGACGTGTTGACGAAAGTGAGGATCACCGAAACGCTGAGGGTGGTGATAACAACACGGCTGAgg > 1:1289407/1‑68 (MQ=255) ccGGTGACGTGTTGACGAAAGTGAGGATCACCGAAACGCTGAGGGTGGTGATAACAACACGGCTGAgg > 1:2931141/1‑68 (MQ=255) ccGGTGACGTGTTGACGAAAGTGAGGATCACCGAAACGCTGAGGGTGGTGATAACAACACGGCTGAgg > 1:2930369/1‑68 (MQ=255) ccGGTGACGTGTTGACGAAAGTGAGGATCACCGAAACGCTGAGGGTGGTGATAACAACACGGCTGAgg > 1:2903043/1‑68 (MQ=255) ccGGTGACGTGTTGACGAAAGTGAGGATCACCGAAACGCTGAGGGTGGTGATAACAACACGGCTGAgg > 1:3168240/1‑68 (MQ=255) ccGGTGACGTGTTGACGAAAGTGAGGATCACCGAAACGCTGAGGGTGGTGATAACAACACGGCTGAgg > 1:1175759/1‑68 (MQ=255) ccGGTGACGTGTTGACGAAAGTGAGGATCACCGAAACGCTGAGGGTGGTGATAACAACACGGCTGAg > 1:1431849/1‑67 (MQ=255) ccGATGACGTGTTGACGAAAGTGAGGATCACCGAAACGCTGAGGGTGGTGATAACAACACGGCTGAgg > 1:3414252/1‑68 (MQ=255) gTGACGTGTTGACGAAAGTGAGGATCACCGAAACGCTGAGGGTGGTGATAACAACACGGCTGAGGaaaa < 1:751565/69‑1 (MQ=255) | TCAGCGCCATAATGGTGGCGTATTCACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACGTGTTGACGAAAGTGAGGATCACCGAAACGCTGAGGGTGGTGATAACAACACGGCTGAGGAAAA > minE/2482125‑2482256 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |