| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,482,204 | A→G | 52.1% | T220T (ACT→ACC) | mdtL ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,482,204 | 0 | A | G | 52.1% | 7.0 / 85.7 | 98 | T220T (ACT→ACC) | mdtL | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base A (21/26); new base G (7/44); total (28/70) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.17e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.16e-01 | |||||||||||
Only 100 of 101 total aligned reads displayed.
GTGGCGTATTCACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACGTGTTGACGAAAGTGAGGATCACCGAAACGCTGAGGGTGGTGATAACAACACGGCTGAGGAAAAAACGGTTAAGCAGCGA > minE/2482139‑2482272 | gtggCGTATTCACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACGTGTTGACGAAAGTg < 1:3190999/69‑1 (MQ=255)gtggCGTATTCACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACGTGTTGACGAAAGTg < 1:600268/69‑1 (MQ=255)gtggCGTATTCACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACGTGTTGACGAAAGTg < 1:1960309/69‑1 (MQ=255)gtggCGTATTCACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACGTGTTGACGAAAGTg < 1:2462341/69‑1 (MQ=255)gtggCGTATTCACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACGTGTTGACGAAAGTg < 1:1915383/69‑1 (MQ=255)gtggCGTATTCACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACGTGTTGACGAAAGTg < 1:1863139/69‑1 (MQ=255)gtggCGTATTCACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACGTGTTGACGAAAGTg < 1:748463/69‑1 (MQ=255)gtggCGTATTCACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACGTGTTGACGAAAGTg < 1:1690509/69‑1 (MQ=255)gtggCGTATTCACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACGTGTTGACGAAAGTg < 1:3228511/69‑1 (MQ=255) tggCGTATTCACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACGTGTTGACGAAAGTg < 1:862877/68‑1 (MQ=255) gCGTATTCACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACGTGTTGACGAAAGTGAgg < 1:1313567/69‑1 (MQ=255) gCGTATTCACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACGTGTTGACGAAAGTGAgg < 1:1338316/69‑1 (MQ=255) gCGTATTCACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACGTGTTGACGAAAGTGAgg < 1:2437200/69‑1 (MQ=255) gCGTATTCACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACGTGTTGACGAAAGTGAgg < 1:473788/69‑1 (MQ=255) gCGTATTCACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACGTGTTGACGAAAGTGAgg < 1:3154711/69‑1 (MQ=255) cACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACGTGTTGACGAAAGTGAgg < 1:3228614/62‑1 (MQ=255) cACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACGTGTTGACGAAAGTGAgg < 1:2143932/62‑1 (MQ=255) cACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACGTGTTGACGAAAGTGAgg < 1:2114962/62‑1 (MQ=255) cACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACGTGTTGACGAAAGTGAgg < 1:949479/62‑1 (MQ=255) cACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACGTGTTGACGAAAGTGAgg < 1:3086857/62‑1 (MQ=255) cACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACGTGTTGACGAAAGTGAgg < 1:3388549/62‑1 (MQ=255) cACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACGTGTTGACGAAAGTGAGGATCAcc > 1:587092/1‑68 (MQ=255) ccGCGCTCATAACCCATGATTTCCATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGa < 1:1721741/68‑1 (MQ=255) ccGCGCTCAAAACCCATGATTTCCATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGa < 1:263152/68‑1 (MQ=255) ccGCGCTCAAAACCCATGATTTCCATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGa < 1:265459/68‑1 (MQ=255) ccGCGCTCAAAACCCATGATTTCCATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGa < 1:3094456/68‑1 (MQ=255) ccGCGCTCAAAACCCATGATTTCCATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGa < 1:2740871/68‑1 (MQ=255) ccGCGCTCAAAACCCATGATTTCCATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGa < 1:2548953/68‑1 (MQ=255) ccGCGCTCAAAACCCATGATTTCCATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGa < 1:2806124/68‑1 (MQ=255) ccGCGCTCAAAACCCATGATTTCCATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGa < 1:486938/68‑1 (MQ=255) ccGCGCTCAAAACCCATGATTTCCATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGa < 1:2440114/68‑1 (MQ=255) ccGCGCTCAAAACCCATGATTTCCATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGa < 1:291700/68‑1 (MQ=255) ccGCGCTCAAAACCCATGATTTCCATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGa < 1:3382354/68‑1 (MQ=255) ccGCGCTCAAAACCCATGATTTCCATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGa < 1:2378098/68‑1 (MQ=255) ccGCGCTCAAAACCCATGATTTCCATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGa < 1:643789/68‑1 (MQ=255) ccGCGCTCAAAACCCATGATTTCCATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGa < 1:55474/68‑1 (MQ=255) ccGCGCTCAAAACCCATGATTTCCATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGa < 1:1380101/68‑1 (MQ=255) ccGCGCTCAAAACCCATGATTTCCATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGa < 1:1128461/68‑1 (MQ=255) ccGCGCTCAAAACCCATGATTTCCATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGa < 1:857700/68‑1 (MQ=255) ccGCGCTCAAAACCCATGATTTCCATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGa < 1:1360103/68‑1 (MQ=255) ccGCGCTCAAAACCCATGATTTCCATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGa < 1:1378847/68‑1 (MQ=255) ccGCGCTCAAAACCCATGATTTCCATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGa < 1:598624/68‑1 (MQ=255) ccGCGCTCAAAACCCATGATTTCCATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGa < 1:1700754/68‑1 (MQ=255) ccGCGCTCAAAACCCATGATTTCCATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGa < 1:687953/68‑1 (MQ=255) cgcgCTCAAAACCCATGATTTCCATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGa < 1:1461750/67‑1 (MQ=255) cgcgCTCAAAACCCATGATTTCCATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGa < 1:743279/67‑1 (MQ=255) cgcgCTCAAAACCCATGATTTCCATCAGCAATACTGGCGACGTGGTGACGAAGGTGAGGATCACCGa < 1:2031473/67‑1 (MQ=255) gATTTCCATCAGCAATACCGGTGACGTGTTGACGAAAGTGAGGATCACCGAAACGCTGAGGGTGGTGAt > 1:800277/1‑69 (MQ=255) gATTTCCATCAGCAATACCGGTGACGTGTTGACGAAAGTGAGGATCACCGAAACGCTGAGGGTGGTGAt > 1:1230940/1‑69 (MQ=255) aTTTCCATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGa < 1:1198781/50‑1 (MQ=255) ttCCATCAGCAATACCGGTGACGTGTTGACGAAAGTGAGGATCACCGAAACGCTGAGGGTGGTGATaac < 1:511183/69‑1 (MQ=255) ccATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGAAACGCTGAGGGTGGTGATaacaa > 1:2673121/1‑69 (MQ=255) ccATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGAAACGCTGAGGGTGGTGATaacaa > 1:978401/1‑69 (MQ=255) ccATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGAAACGCTGAGGGTGGTGATaacaa > 1:3288348/1‑69 (MQ=255) ccATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGAAACGCTGAGGGTGGTGATaacaa > 1:1350549/1‑69 (MQ=255) ccATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGAAACGCTGAGGGTGGTGATaacaa > 1:3421305/1‑69 (MQ=255) ccATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGAAACGCTGAGGGTGGTGATaacaa > 1:277464/1‑69 (MQ=255) ccATCAGCAATACTGGCGAAGTGTTGACGAAGGTGAGGATCACCGaa > 1:3296321/1‑47 (MQ=255) cATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGAAACTCTGAGGGTGGTGATaacaa < 1:808052/68‑1 (MQ=255) cATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGAAACGCTGAGGGTGGTGATaacaa < 1:3338045/68‑1 (MQ=255) cATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGAAACGCTGAGGGTGGTGATaacaa < 1:3280749/68‑1 (MQ=255) cATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGAAACGCTGAGGGTGGTGATaacaa < 1:81172/68‑1 (MQ=255) cATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGAAACGCTGAGGGTGGTGATaacaa < 1:694649/68‑1 (MQ=255) cATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGAAACGCTGAGGGTGGTGATaacaa < 1:765972/68‑1 (MQ=255) cATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGAAACGCTGAGGGTGGTGATaacaa < 1:1156413/68‑1 (MQ=255) cATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGAAACGCTGAGGGTGGTGATaacaa < 1:322371/68‑1 (MQ=255) cATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGAAACGCTGAGGGTGGTGATaacaa < 1:204735/68‑1 (MQ=255) cATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGAAACGCTGAGGGTGGTGATaacaa < 1:1923694/68‑1 (MQ=255) cATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGAAACGCTGAGGGTGGTGATaacaa < 1:2336841/68‑1 (MQ=255) cATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGAAACGCTGAGGGTGGTGATaacaa < 1:1919110/68‑1 (MQ=255) cATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGAAACGCTGAGGGTGGTGATaacaa < 1:1719173/68‑1 (MQ=255) cATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGAAACGCTGAGGGTGGTGATaacaa < 1:1595561/68‑1 (MQ=255) cATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGAAACGCTGAGGGTGGTGATaacaa < 1:3033054/68‑1 (MQ=255) cATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGGATCACCGAAACGCTGAGGGTGGTGATaacaa < 1:117549/68‑1 (MQ=255) cATCAGCAATACTGGCGACGTGTTGACGAAGGTGAGCATCACCGAAACGCTGAGGGTGGTGATaacaa < 1:2932546/68‑1 (MQ=255) cATCAGCAATACCGGTGACGTGTTGACGAAAGTGAGGATCACCGAAACGCTGAGGGTGGTGATaacaa < 1:2849904/68‑1 (MQ=255) cATCAGCAATACCGGTGACGTGTTGACGAAAGTGAGGATCACCGAAACGCTGAGGGTGGTGATaacaa < 1:3083725/68‑1 (MQ=255) aaTACCGGTGACGTGTTGACGAAAGTGAGGATCACCGAAACGCTGAGGGTGGTGATAACAACACGGCTg > 1:3006054/1‑69 (MQ=255) aaTACCGGTGACGTGTTGACGAAAGTGAGGATCACCGAAACGCTGAGGGTGGTGATAACAACACGGCTg > 1:1270520/1‑69 (MQ=255) aaTACCGGTGACGTGTTGACGAAAGTGAGGATCACCGAAACGCTGAGGGTGGTGATAACAACACGGCTg > 1:3394240/1‑69 (MQ=255) aaTACCGGTGACGTGTTGACGAAAGTGAGGATCACCGAAACGCTGAGGGTGGTGATAACAACACGGCTg > 1:2246377/1‑69 (MQ=255) aaTACCGGTGACGTGTTGACGAAAGTGAGGATCACCGAAACGCTGAGGGTGGTGATAACAACACGGCTg > 1:217861/1‑69 (MQ=255) aTACTGGCGACGTGTTGACGAAGGTGAGGATCACCGa < 1:1817353/37‑1 (MQ=25) ccGGTGACGTGTTGACGAAAGTGAGGATCACCGAAACGCTGAGGGTGGTGATAacaaca > 1:3262022/1‑59 (MQ=255) ccGGTGACGTGTTGACGAAAGTGAGGATCACCGAAACGCTGAGGGTGGTGATAACAACACGGCTGAgg > 1:1289407/1‑68 (MQ=255) ccGGTGACGTGTTGACGAAAGTGAGGATCACCGAAACGCTGAGGGTGGTGATAACAACACGGCTGAgg > 1:1175759/1‑68 (MQ=255) ccGGTGACGTGTTGACGAAAGTGAGGATCACCGAAACGCTGAGGGTGGTGATAACAACACGGCTGAgg > 1:3168240/1‑68 (MQ=255) ccGGTGACGTGTTGACGAAAGTGAGGATCACCGAAACGCTGAGGGTGGTGATAACAACACGGCTGAgg > 1:2159130/1‑68 (MQ=255) ccGGTGACGTGTTGACGAAAGTGAGGATCACCGAAACGCTGAGGGTGGTGATAACAACACGGCTGAgg > 1:545469/1‑68 (MQ=255) ccGGTGACGTGTTGACGAAAGTGAGGATCACCGAAACGCTGAGGGTGGTGATAACAACACGGCTGAgg > 1:2903043/1‑68 (MQ=255) ccGGTGACGTGTTGACGAAAGTGAGGATCACCGAAACGCTGAGGGTGGTGATAACAACACGGCTGAgg > 1:2930369/1‑68 (MQ=255) ccGGTGACGTGTTGACGAAAGTGAGGATCACCGAAACGCTGAGGGTGGTGATAACAACACGGCTGAgg > 1:2931141/1‑68 (MQ=255) ccGGTGACGTGTTGACGAAAGTGAGGATCACCGAAACGCTGAGGGTGGTGATAACAACACGGCTGAgg > 1:3095023/1‑68 (MQ=255) ccGGTGACGTGTTGACGAAAGTGAGGATCACCGAAACGCTGAGGGTGGTGATAACAACACGGCTGAg > 1:2868784/1‑67 (MQ=255) ccGGTGACGTGTTGACGAAAGTGAGGATCACCGAAACGCTGAGGGTGGTGATAACAACACGGCTGAg > 1:1431849/1‑67 (MQ=255) ccGATGACGTGTTGACGAAAGTGAGGATCACCGAAACGCTGAGGGTGGTGATAACAACACGGCTGAgg > 1:3414252/1‑68 (MQ=255) gTGACGTGTTGACGAAAGTGAGGATCACCGAAACGCTGAGGGTGGTGATAACAACACGGCTGAGGaaaa < 1:751565/69‑1 (MQ=255) aGTGAGGATCACCGAAACGCTGAGGGTGGTGATAACAACACGGCTGAGGAAAAAACGGTTAAGCAGCGa > 1:2332324/1‑69 (MQ=255) aGTGAGGATCACCGAAACGCTGAGGGTGGTGATAACAACACGGCTGAGGAAAAAACGGTTAAGCAGCGa > 1:3063741/1‑69 (MQ=255) aGTGAGGATCACCGAAACGCTGAGGGTGGTGATAACAACACGGCTGAGGAAAAAACGGTTAAGCAGCGa > 1:1175383/1‑69 (MQ=255) | GTGGCGTATTCACCGCGCTCAAACCCCATGATTTCCATCAGCAATACCGGTGACGTGTTGACGAAAGTGAGGATCACCGAAACGCTGAGGGTGGTGATAACAACACGGCTGAGGAAAAAACGGTTAAGCAGCGA > minE/2482139‑2482272 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |