| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 27,925 | T→C | 49.2% | F199F (TTT→TTC) | carA → | carbamoyl phosphate synthetase small subunit, glutamine amidotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 27,925 | 0 | T | C | 49.2% | ‑5.8 / 53.4 | 59 | F199F (TTT→TTC) | carA | carbamoyl phosphate synthetase small subunit, glutamine amidotransferase |
| Reads supporting (aligned to +/- strand): ref base T (8/22); new base C (13/16); total (21/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTGGCCTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGCTTATGATTTTGGTGCCAAGCGCAACATCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCGG > minE/27864‑27989 | gTGGCCTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGCTTATGATTTTGGTGCCa < 1:1497018/69‑1 (MQ=255)gTGGCCTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGCTTATGATTTTGGTGCCa < 1:1258392/69‑1 (MQ=255) gCCTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGCTTATGATTTTGGTGCCAAg > 1:2198421/1‑68 (MQ=255) gCCTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGCTTATGATTTTGGTGCCAAg > 1:1514062/1‑68 (MQ=255) gCCTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGCTTATGATTTTGGTGCCAAg > 1:1318820/1‑68 (MQ=255) gCCTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGCTTATGATTTTGGTGCCAAg > 1:165973/1‑68 (MQ=255) ccAGAAGCGAAAAAAGAAGACGATCTGCCGTTCCACGTCGTGGCTTATGATTTTGGTGCCAAGCGCAAc > 1:2912213/1‑69 (MQ=255) ccAGAAGCGAAAAAAGAAGACGAGCTGCAGTTCCACGTCGTGGCTTATGATTTTGGTGCCAAGCGCaa > 1:1910957/1‑68 (MQ=255) aGAAGCGAAAAAAGAAGACGATCTGCCGTTCCACGTCGTGGCTTATGATTTTGGTGCCAAGCGCAACAt > 1:335807/1‑69 (MQ=255) aGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGCTTATGATTTTGGTGCCAAGCGCAACAt < 1:3301528/69‑1 (MQ=255) aGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGCTTATGATTTTGGTGCCAAGCGCAACAt < 1:652123/69‑1 (MQ=255) aGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGCTTATGATTTTGGTGCCAAGCGCAACAt < 1:1954986/69‑1 (MQ=255) aGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGCTTATGATTTTGGTGCCAAGCGCAACAt < 1:660742/69‑1 (MQ=255) aGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGCTTATGATTTTGGTGCCAAGCGCAACAt < 1:2224209/69‑1 (MQ=255) aGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGCTTATGATTTTGGTGCCAAGCGCAACAt > 1:820086/1‑69 (MQ=255) taaaaaaGAAGACGAGCTGCCGTTCCACGTCGTGGCTTATGATTTTGGTGCCAAGCGCAACATCCTGCg < 1:511457/68‑1 (MQ=255) gAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGCTTATGATTTTGGTGCCAAGCGCAACATCCTGCg < 1:489108/69‑1 (MQ=255) gAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGCTTATGATTTTGGTGCCAAGCGCAACATCCTGCg < 1:1474538/69‑1 (MQ=255) gAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGCTTATGATTTTGGTGCCAAGCGCAACATCCTGCg < 1:385077/69‑1 (MQ=255) caaaaaaGAAGACGAGCTGCCGTTCCACGTCGTGGCTTATGATTTTGGTGCCAAGCGCAACATCCTGCg < 1:2088484/68‑1 (MQ=255) aaaGAAGACGAGCTGCCGTTCCACGTCGTGGCTTATGATTTTGGTGCCAAGCGCAACAt < 1:773221/59‑1 (MQ=255) gaCGAGCTGCCGTTCCACGTCGTGGCTTATGATTTTGGTGCCAAGCTCAACATCCTGCg < 1:2924259/59‑1 (MQ=255) cGTACCACGTCGTGGCCTACGATTTCGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGc > 1:905167/1‑68 (MQ=255) cGTACCACGTCGTGGCCTACGATTTCGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCt > 1:2080112/1‑69 (MQ=255) cGTACCACGTCGTGGCCTACGATTTCGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCt > 1:983571/1‑69 (MQ=255) cGTACCACGTCGTGGCCTACGATTTCGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCt > 1:2063700/1‑69 (MQ=255) cGTACCACGTCGTGGCCTACGATTTCGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCt > 1:2095878/1‑69 (MQ=255) cGTACCACGTCGTGGCCTACGATTTCGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCt > 1:2306908/1‑69 (MQ=255) cGTACCACGTCGTGGCCTACGATTTCGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCt > 1:2418182/1‑69 (MQ=255) cGTACCACGTCGTGGCCTACGATTTCGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCt > 1:3312210/1‑69 (MQ=255) cGTACCACGTCGTGGCCTACGATTTCGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCt > 1:381746/1‑69 (MQ=255) cGTACCACGTCGTGGCCTACGATTTCGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCt > 1:1226766/1‑69 (MQ=255) cGTACCACGTCGTGGCCTACGATTTCGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCt > 1:1257741/1‑69 (MQ=255) cGTACCACGTCGTGGCCTACGATTTCGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCt > 1:1341460/1‑69 (MQ=255) cGTACCACGTCGTGGCCTACGATTTCGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCt > 1:118354/1‑69 (MQ=255) ccACGTCGTGGCTTATGATTTTGGTGCCAAGCGCAACATCCTGCg < 1:3232360/45‑1 (MQ=255) ccACGTCGTGGCTTATGATTTTGGTGCCAAGCGCAACATCCTGCg < 1:1685783/45‑1 (MQ=255) ccACGTCGTGGCTTATGATTTTGGTGCCAAGCGCAACATCCTGCg < 1:923358/45‑1 (MQ=255) ccACGTCGTGGCTTATGATTTTGGTGCCAAGCGCAACATCCTGCg < 1:37631/45‑1 (MQ=255) ccACGTCGTGGCTTATGATTTTGGTGCCAAGCGCAACATCCTGCg < 1:706895/45‑1 (MQ=255) ccACGTCGTGGCTTATGATTTTGGTGCCAAGCGCAACATCCTGCg < 1:2763343/45‑1 (MQ=255) cACGTCGTGGCTTATGATTTTGGTGCCAAGCGCAACATCCTGCg < 1:1153947/44‑1 (MQ=255) tGGCTTATGATTTTGGTGCCAAGCGCAACATCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGAcc < 1:1524151/68‑1 (MQ=255) aTTTCGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATTGTTcc < 1:536223/67‑1 (MQ=255) aTTTCGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTcc < 1:3159186/67‑1 (MQ=255) aTTTCGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTcc < 1:1172556/67‑1 (MQ=255) aTTTCGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTcc < 1:2585471/67‑1 (MQ=255) aTTTCGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTcc < 1:1255066/67‑1 (MQ=255) aTTTCGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTcc < 1:2855359/67‑1 (MQ=255) aTTTCGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTcc < 1:1277436/67‑1 (MQ=255) aTTTCGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTcc < 1:2621948/67‑1 (MQ=255) aTTTCGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTcc < 1:2570243/67‑1 (MQ=255) aTTTCGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTcc < 1:2481428/67‑1 (MQ=255) aTTTCGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTcc < 1:241167/67‑1 (MQ=255) aTTTCGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTcc < 1:1547208/67‑1 (MQ=255) aTTTCGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTcc < 1:2221872/67‑1 (MQ=255) aTTTCGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTcc < 1:167098/67‑1 (MQ=255) aTTTCGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTcc < 1:1791927/67‑1 (MQ=255) aTTTCGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTcc < 1:1978140/67‑1 (MQ=255) tttcGGTGCCAAGCGCAACATCCTGCGTATGCTGGTTGATAGAGGCTGTCGCCTGACCATCGTTCCgg > 1:2035946/1‑68 (MQ=255) tttcGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGAt > 1:1174494/1‑40 (MQ=255) tttcGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTcc < 1:3174498/66‑1 (MQ=255) tttcGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCgg > 1:1066236/1‑68 (MQ=255) tttcGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCgg > 1:937336/1‑68 (MQ=255) tttcGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCgg > 1:747106/1‑68 (MQ=255) tttcGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCgg > 1:729380/1‑68 (MQ=255) tttcGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCgg > 1:738227/1‑68 (MQ=255) tttcGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCgg > 1:305675/1‑68 (MQ=255) tttcGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCgg > 1:1052809/1‑68 (MQ=255) tttcGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCgg > 1:1107778/1‑68 (MQ=255) tttcGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCgg > 1:1326253/1‑68 (MQ=255) tttcGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCgg > 1:1332106/1‑68 (MQ=255) tttcGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCgg > 1:1866263/1‑68 (MQ=255) tttcGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCgg > 1:203827/1‑68 (MQ=255) tttcGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCgg > 1:2042529/1‑68 (MQ=255) tttcGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCgg > 1:2450550/1‑68 (MQ=255) tttcGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCgg > 1:2626025/1‑68 (MQ=255) tttcGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCgg > 1:2627558/1‑68 (MQ=255) tttcGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCgg > 1:2695621/1‑68 (MQ=255) tttcGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCgg > 1:3045109/1‑68 (MQ=255) tttcGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCgg > 1:3420754/1‑68 (MQ=255) tttcGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCAgg > 1:1669489/1‑68 (MQ=255) tttcGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTAGCCTGACCATCGTTCCgg > 1:987166/1‑68 (MQ=255) tttcGGTGCCAAGAGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCgg > 1:122607/1‑68 (MQ=255) | GTGGCCTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGCTTATGATTTTGGTGCCAAGCGCAACATCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCGG > minE/27864‑27989 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |