| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,482,270 | C→T | 27.4% | S198S (TCG→TCA) | mdtL ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,482,270 | 0 | C | T | 27.4% | 63.1 / 20.7 | 58 | S198S (TCG→TCA) | mdtL | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base C (19/23); new base T (4/12); total (23/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGTGAGGATCACCGAAACGCTGAGGGTGGTGATAACAACACGGCTGAGGAAAAAACGGTTAAGCAGCGACTCGCTATTTTCTCGTGGTTTATCCGAAGCTGCGGGGGC > minE/2482204‑2482311 | aGTGAGGATCACCGAAACGCTGAGGGTGGTGATAACAACACGGCTGAGGAAAAAACGGTTAAGCAGCGa > 1:1175383/1‑69 (MQ=255)aGTGAGGATCACCGAAACGCTGAGGGTGGTGATAACAACACGGCTGAGGAAAAAACGGTTAAGCAGCGa > 1:3063741/1‑69 (MQ=255)aGTGAGGATCACCGAAACGCTGAGGGTGGTGATAACAACACGGCTGAGGAAAAAACGGTTAAGCAGCGa > 1:2332324/1‑69 (MQ=255) cACCGAAACGCTGAGGGTGGTGATAACAACACGGCTGAGGAAAAACCGGTTAAGCAGTGACTCGCTAtt > 1:584686/1‑69 (MQ=255) cACCGAAACGCTGAGGGTGGTGATAACAACACGGCTGAGGAAAAACCGGTTAAGCAGTGACTCGCTAtt > 1:557914/1‑69 (MQ=255) cACCGAAACGCTGAGGGTGGTGATAACAACACGGCTGAGGAAAAACCGGTTAAGCAGTGACTCGCTAtt > 1:2437668/1‑69 (MQ=255) cACCGAAACGCTGAGGGTGGTGATAACAACACGGCTGAGGAAAAACCGGTTAAGCAGTGACTCGCTAt > 1:2464514/1‑68 (MQ=255) cACCGAAACGCTGAGGGTGGTGATAACAACACGGCTGAGGAAAAAACGGTTAAGCAGCGACTCGCTAtt > 1:1723446/1‑69 (MQ=255) gAGGGTGGTGATAACAACACGGCTGAGGAAAAAACGGTTAAGCAGCGACTCGCTATTTTCTCGTGGttt < 1:2940164/69‑1 (MQ=255) gAGGGTGGTGATAACAACACGGCTGAGGAAAAAACGGTTAAGCAGCGACTCGCTATTTTCTCGTGGttt < 1:2956887/69‑1 (MQ=255) gAGGGTGGTGATAACAACACGGCTGAGGAAAAAACGGTTAAGCAGCGACTCGCTATTTTCTCGTGGttt < 1:219820/69‑1 (MQ=255) gAGGGTGGTGATAACAACACGGCTGAGGAAAAAACGGTTAAGCAGCGACTCGCTATTTTCTCGTGGttt < 1:3397205/69‑1 (MQ=255) gAGGGTGGTGATAACAACACGGCTGAGGAAAAAACGGTTAAGCAGCGACTCGCTATTTTCTCGTGGttt < 1:904602/69‑1 (MQ=255) gtggtgATAACAACACGGCTGAGGAAAAAACGGTTAAGCAGCGACTCGCTATTTTCTCGTGGTTTATcc < 1:1970604/69‑1 (MQ=255) gtggtgATAACAACACGGCTGAGGAAAAAACGGTTAAGCAGCGACTCGCTATTTTCTCGTGGTTTATcc < 1:2346173/69‑1 (MQ=255) gtggtgATAACAACACGGCTGAGGAAAAAACGGTTAAGCAGCGACTCGCTATTTTCTCGTGGTTTATcc < 1:1641972/69‑1 (MQ=255) gtggtgATAACAACACGGCTGAGGAAAAAACGGTTAAGCAGCGACTCGCTATTTTCTCGTGGTTTATcc < 1:678493/69‑1 (MQ=255) gtgATAACAACACGGCTGAGGAAAAAACGGTTAAGCAGCGACTCGCTATTTTCTCGTGGTTTATCCGaa > 1:1621028/1‑69 (MQ=255) gATAACAACACGGCTGAGGAAAAAACGGTTAAGCAGCGACTCGCTATTTTCTCGTGGTTTATCCGAAGc < 1:2074823/69‑1 (MQ=255) gATAACAACACGGCTGAGGAAAAAACGGTTAAGCAGCGACTCGCTATTTTCTCGTGGTTTATCCGAAGc < 1:2805325/69‑1 (MQ=255) aTAACAACACGGCTGAGGAAAAAACGGTTAAGCAGCGACTCGCTATTTTCTCGTGGTTTATcc < 1:431761/63‑1 (MQ=255) aTAACAACACGGCTGAGGAAAAAACGGTTAAGCAGCGACTCGCTATTTTCTCGTGGTTTATCCGAAGc > 1:122533/1‑68 (MQ=255) aTAACAACACGGCTGAGGAAAAAACGGTTAAGCAGCGACTCGCTATTTTCTCGTGGTTTATCCGAAGCt > 1:428201/1‑69 (MQ=255) aTAACAACACGGCTGAGGAAAAAACGGTTAAGCAGCGACTCGCTATTTTCTCGTGGTTTATCCGAAGCt > 1:1068315/1‑69 (MQ=255) aTAACAACACGGCTGAGGAAAAAACGGTTAAGCAGCGACTCGCTATTTTCTCGTGGTTTATCCGAAGCt > 1:534834/1‑69 (MQ=255) aTAACAACACGGCTGAGGAAAAAACGGTTAAGCAGCGACTCGCTATTTTCTCGTGGTTTATCCGAAGCt > 1:754244/1‑69 (MQ=255) aTAACAACACGGCTGAGGAAAAAACGGTTAAGCAGCGACTCGCTATTTTCTCGTGGTTTATCCGAAGCt > 1:2696632/1‑69 (MQ=255) aTAACAACACGGCTGAGGAAAAAACGGTTAAGCAGCGACTCGATATTTTCTCGTGGTTTATCCGAAGCt > 1:1488553/1‑69 (MQ=255) aacaacACGGCTGAGGAAAAAACGGTTAAGCAGCGACTCGCTATTTtctc < 1:3236166/50‑1 (MQ=255) aacaacACGGCTGAGGAAAAAACGGTTAAGCAGCGACTCGCTATTTTCTCGTGGTTTATCCGAAGCTGc < 1:259402/69‑1 (MQ=255) aacaacACGGCTGAGGAAAAAACGGTTAAGCAGCGACTCGCTATTTTCTCGTGGTTTATCCGAAGCTGc > 1:2801323/1‑69 (MQ=255) aacaacACGGCTGAGGAAAAAACGGTTAAGCAGCGACTCGCTATTTTCTCGTGGTTTATCCGAAGCTGc < 1:2117345/69‑1 (MQ=255) aacaacACGGCTGAGGAAAAAACGGTTAAGCAGCGACTCGCTATTTTCTCGTGGTTTATCCGAAGCTGc < 1:3102952/69‑1 (MQ=255) aacaacACGGCTGAGGAAAAAACGGTTAAGCAGCGACTCGCTATTTTCTCGTGGTTTATCCGAAGCTGc > 1:3099881/1‑69 (MQ=255) acaacaCGGCTGAGGAAAAAACGGTTAAGCAGCGACTCGCTATTTTCTCGTGGTTTATCCGa < 1:2673332/62‑1 (MQ=255) acaacaCGGCTGAGGAAAAAACGGTTAAGCAGCGACTCGCTATTTTCTCGTGGTTTATCCGa < 1:2201608/62‑1 (MQ=255) acaacaCGGCTGAGGAAAAAACGGTTAAGCAGCGACTCGCTATTTTCTCGTGGTTTATCCGAAGCTGc < 1:2744763/68‑1 (MQ=255) acaacaCGGCTGAGGAAAAAACGGTTAAGCAGCGACTCGCTATTTTCTCGTGGTTTATCCGAAGCTGc < 1:2167016/68‑1 (MQ=255) acaacaCGGCTGAGGAAAAAACGGTTAAGCAGCGACTCGCTATTTTCTCGTGGTTTATCCGAAGCTGc < 1:2856963/68‑1 (MQ=255) acaacaCGGCTGAGGAAAAAACGGTTAAGCAGCGACTCGCTATTTTCTCGTGGTTTATCCGAAGCTGc < 1:2208445/68‑1 (MQ=255) acaacaCGGCTGAGGAAAAAACGGTTAAGCAGCGACTCGCTATTTTCTCGTGGTTTATCCGAAGCTGCg > 1:944372/1‑69 (MQ=255) acaacaCGGCTGAGGAAAAAACGGTTAAGCAGCGACTCGCTATTTTCTCGTGGTTTATCCGAAGCTGCg > 1:1242509/1‑69 (MQ=255) acaacaCGGCTGAGGAAAAAACGGTTAAGCAGCGACTCGCTATTTTCTCGTGGTTTATCCGAAGCTGCg > 1:3371213/1‑69 (MQ=255) acaacaCGGCTGAGGAAAAAACGGTTAAGCAGCGACTCGCTATTTTCTCGTGGTTTATCCGAAGCTGCg > 1:3057937/1‑69 (MQ=255) acGGCTGAGGAAAAAACGGTTAAGCAGCGACTCGCTATTTTCTCGTGGTTTATCCGAAGCTGCGGGGGc > 1:2677657/1‑69 (MQ=255) ggAAAAAACGGTTAAGCAGCGACTCGCTATTTTCTCGTGGTTTATCCGa < 1:928044/49‑1 (MQ=255) aaaacCTGTTAAGCAGTGACTCGCTATTTTCTCGTGGTTTGTCCGa < 1:3303664/46‑1 (MQ=255) aaaacCGGTTAAGCAGTGACTCGCTATTTTCTCGTGGTTTGTCCGa < 1:2884773/46‑1 (MQ=255) aaaacCGGTTAAGCAGTGACTCGCTATTTTCTCGTGGTTTGTCCGa < 1:2824119/46‑1 (MQ=255) aaaacCGGTTAAGCAGTGACTCGCTATTTTCTCGTGGTTTGTCCGa < 1:1996437/46‑1 (MQ=255) aaaacCGGTTAAGCAGTGACTCGCTATTTTCTCGTGGTTTGTCCGa < 1:1907851/46‑1 (MQ=255) aaaacCGGTTAAGCAGTGACTCGCTATTTTCTCGTGGTTTGTCCGa < 1:2740910/46‑1 (MQ=255) aaaacCGGTTAAGCAGTGACTCGCTATTTTCTCGTGGTTTGTCCGa < 1:2719513/46‑1 (MQ=255) aaaacCGGTTAAGCAGTGACTCGCTATTTTCTCGTGGTTTGTCCGa < 1:1198889/46‑1 (MQ=255) aaaacCGGTTAAGCAGTGACTCGCTATTTTCTCGTGGTTTGTCCGa < 1:1027772/46‑1 (MQ=255) aaacCGGTTAAGCAGTGACTCGCTATTTTCTCGTGGTTTGTCCGa < 1:2175236/45‑1 (MQ=255) aaacCGGTTAAGCAGTGACTCGCTATTTTCTCGTGGTTTGTCCGa < 1:2454630/45‑1 (MQ=255) aaacCGGTTAAGCAGTGACTCGCTATTTTCTCGTGGTTTGTCCGa < 1:1092945/45‑1 (MQ=255) | AGTGAGGATCACCGAAACGCTGAGGGTGGTGATAACAACACGGCTGAGGAAAAAACGGTTAAGCAGCGACTCGCTATTTTCTCGTGGTTTATCCGAAGCTGCGGGGGC > minE/2482204‑2482311 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |