| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,483,575 | T→C | 69.3% | T307T (ACA→ACG) | trmE ← | GTPase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,483,575 | 0 | T | C | 69.3% | 20.0 / 8.3 | 26 | T307T (ACA→ACG) | trmE | GTPase |
| Reads supporting (aligned to +/- strand): ref base T (2/6); new base C (13/5); total (15/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.84e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01 | |||||||||||
TTCGCTGGCAGACGGGCAATAAATTCCGGCCAGATCTCTGCCGGATCCACGGCGTCTGTTGTGGTGCCATCGACCATAAACAGCACGCGGTCGGCCTGTTCAATTTCCTGCCACGCGCGCTC > minE/2483516‑2483637 | ttCGCTGGCAGACGGGCAATAAATTCCGGCCAGATCTCTGCCGGATCCACGGCGTCtgttgt < 1:661935/62‑1 (MQ=255)ttCGCTGGCAGACGGGCAATAAATTCCGGCCAGATCTCTGCCGGATCCACGGCGTCtgttgt < 1:2177102/62‑1 (MQ=255) ggCAGACGGGCAATAAATTCCGGCCAGATCTCTGCCGGATCCACGGCGTCTGTTgtggt > 1:2094733/1‑59 (MQ=255) ggCAGACGGGCAATAAATTCCGGCCAGATCTCTGCCGGATCCACGGCGTCTGTTGTGGTGCCATCGAc > 1:3170401/1‑68 (MQ=255) aGACGGGTGATAAATTCCGGCCAGATCTCTGCCGGATCAACGTCGTCTGTCGTGGTGCCATCGACCATa < 1:334976/69‑1 (MQ=255) aGACGGGCGATAAATTCCGGCCAGATCTCTGCCGGATCAACGTCGTCTGTCGTGGTGCCATCGACCATa < 1:257295/69‑1 (MQ=255) aGACGGGCGATAAATTCCGGCCAGATCTCTGCCGGATCAACGTCGTCTGTCGTGGTGCCATCGACCATa < 1:92771/69‑1 (MQ=255) aGACGGGCGATAAATTCCGGCCAGATCTCTGCCGGATCAACGTCGTCTGTCGTGGTGCCATCGACCATa < 1:2281587/69‑1 (MQ=255) aGACGGGCGATAAATTCCGGCCAGATCTCTGCCGGATCAACGTCGTCTGTCGTGGTGCCATCGACCATa < 1:1835681/69‑1 (MQ=255) aGACGGGCAATAAATTCCGGCCAGATCTCTGCCGGATCCACGGCGTCTGTTGTGGTGCCATCGACCATa < 1:2426295/69‑1 (MQ=255) cGGGCAATAAATTCCGGCCAGATCTCTGCCGGATCCACGGCGTCTGTTGTGGTGCCATCGACCATAAAc < 1:3102064/69‑1 (MQ=255) cGGGCAATAAATTCCGGCCAGATCTCTGCCGGATCCACGGCGTCTGTTGTGGTGCCATCGACCATAAAc < 1:2187614/69‑1 (MQ=255) tCCGGCCAGATCTCTGCCGGATCAACGGCGTCTGTCGTGGTGCCATCGACCATaa > 1:3102699/1‑55 (MQ=255) tCCGGCCAGATCTCTGCCGGATCAACGGCGTCTGTCGTGGTGCCATCGACCATaa > 1:1482692/1‑55 (MQ=255) tCCGGCCAGATCTCTGCCGGATCAACGGCGTCTGTCGTGGTGCCATCGACCATaa > 1:915690/1‑55 (MQ=255) tCCGGCCAGATCTCTGCCGGATCAACGGCGTCTGTCGTGGTGCCATCGACCATaa > 1:745260/1‑55 (MQ=255) tCCGGCCAGATCTCTGCCGGATCAACGGCGTCTGTCGTGGTGCCATCGACCATaa > 1:678624/1‑55 (MQ=255) tCCGGCCAGATCTCTGCCGGATCAACGGCGTCTGTCGTGGTGCCATCGACCATaa > 1:1484087/1‑55 (MQ=255) tCCGGCCAGATCTCTGCCGGATCAACGGCGTCTGTCGTGGTGCCATCGACCATaa > 1:514860/1‑55 (MQ=255) tCCGGCCAGATCTCTGCCGGATCAACGGCGTCTGTCGTGGTGCCATCGACCATaa > 1:280552/1‑55 (MQ=255) tCCGGCCAGATCTCTGCCGGATCAACGGCGTCTGTCGTGGTGCCATCGACCATaa > 1:1086402/1‑55 (MQ=255) tCCGGCCAGATCTCTGCCGGATCAACGGCGTCTGTCGTGGTGCCATCGACCATaa > 1:2558059/1‑55 (MQ=255) tCCGGCCAGATCTCTGCCGGATCAACGGCGTCTGTCGTGGTGCCATCGACCATaa > 1:2379900/1‑55 (MQ=255) tCCGGCCAGATCTCTGCCGGATCAACGGCGTCTGTCGTGGTGCCATCGACCATaa > 1:2374309/1‑55 (MQ=255) tCCGGCCAGATCTCTGCCGGATCAACGGCGTCTGTCGTGGTGCCATCGACCATa > 1:2739243/1‑54 (MQ=39) aTCTCTGCCGGATCCACGGCGTCTGTTGTGGTGCCATCGACCATAAACAGCACGCGGTCGGCCTGTTCa < 1:155531/69‑1 (MQ=255) gTCTGTTGTGGTGCCATCGACCATAAACAGCACGCGGTCGGCCTGTTCAATTTCCTGCCACGCGCGCTc < 1:3327922/69‑1 (MQ=255) gTCTGTTGTGGTGCCATCGACCATAAACAGCACGCGGTCGGCCTGTTCAATTTCCTGCCACGCGCGCTc < 1:218048/69‑1 (MQ=255) | TTCGCTGGCAGACGGGCAATAAATTCCGGCCAGATCTCTGCCGGATCCACGGCGTCTGTTGTGGTGCCATCGACCATAAACAGCACGCGGTCGGCCTGTTCAATTTCCTGCCACGCGCGCTC > minE/2483516‑2483637 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |