| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,484,871 | C→A | 46.4% | S459S (TCG→TCT) | yidC ← | cytoplasmic insertase into membrane protein, Sec system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,484,871 | 0 | C | A | 46.4% | 33.4 / 100.0 | 97 | S459S (TCG→TCT) | yidC | cytoplasmic insertase into membrane protein, Sec system |
| Reads supporting (aligned to +/- strand): ref base C (20/32); new base A (20/25); total (40/57) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
Only 100 of 112 total aligned reads displayed.
GACATCTTCTGAATGAAGAACATCGTTACGCCCATCAGGATCGGCAGGATGTAGTACGGGTCCTGTGCCGACAGGTCGTGGATCCACAGTGCAAACGGTGCCTGACGCAGTTCAACGGAACCCATCAGCATGTAG > minE/2484803‑2484937 | gACATCTTCTGAATGAAGAACATCGTTACGCCCATCAGGATCGGCAGGATGTAGTACGGGTCCTGTGcc < 1:3037365/69‑1 (MQ=255) ttctGAATGAAGAACATCGTTACGCCCATCAGGATCGGCAGGATGTAGTACGGGTCCTGTGCCGACAgg > 1:2343144/1‑69 (MQ=255) ttctGAATGAAGAACATCGTTACGCCCATCAGGATCGGCAGGATGTAGTACGGGTCCTGTGCCGACAgg > 1:1043051/1‑69 (MQ=255) ttctGAATGAAGAACATCGTTACGCCCATCAGGATCGGCAGGATGTAGTACGGGTCCTGTGCCGACAgg > 1:2056907/1‑69 (MQ=255) ttctGAATGAAGAACATCGTTACGCCCATCAGGATCGGCAGGATGTAGTACGGGTCCTGTGCCGACAgg > 1:758063/1‑69 (MQ=255) ttctGAATGAAGAACATCGTTACGCCCATCAGGATCGGCAGGATGTAGTACGGGTCCTGTGCCGACAgg > 1:530042/1‑69 (MQ=255) ttctGAATGAAGAACATCGTTACGCCCATCAGGATCGGCAGGATGTAGTACGGGTCCTGTGCCGACAgg > 1:588374/1‑69 (MQ=255) ttctGAATGAAGAACATCGTTACGCCCATCAGGATCGGCAGGATGTAGTACGGGTCCTGTGCCGACAgg > 1:2908874/1‑69 (MQ=255) ttctGAATGAAGAACATCGTTACGCCCATCAGGATCGGCAGGATGTAGTACGGGTCCTGTGCCGACAg > 1:2048259/1‑68 (MQ=255) atgaaGAACATCGTTACGCCCATCAGGATCGGCAGGATGTAGTACGGGTCCTGTGCCGACAGGTCGTg < 1:904992/68‑1 (MQ=255) tgaaGAACATCGTTACGCCCATCAGGATCGGCAGGATGTAGTACGGGTCCTGTGCCGACAGGTCGTg < 1:2199643/67‑1 (MQ=255) tgaaGAACATCGTTACGCCCATCAGGATCGGCAGGATGTAGTACGGGTCCTGTGCCGACAGGTCGTg < 1:1058298/67‑1 (MQ=255) tgaaGAACATCGTTACGCCCATCAGGATCGGCAGGATGTAGTACGGGTCCTGTGCCGACAGGTCGTg < 1:710190/67‑1 (MQ=255) tgaaGAACATCGTTACGCCCATCAGGATCGGCAGGATGTAGTACGGGTCCTGTGCCGACAGGTCGTGGa < 1:2906798/69‑1 (MQ=255) tgaaGAACATCGTTACGCCCATCAGGATCGGCAGGATGTAGTACGGGTCCTGTGCCGACAGGTCGTGGa < 1:1324521/69‑1 (MQ=255) tgaaGAACATCGTTACGCCCATCAGGATCGGCAGGATGTAGTACGGGTCCTGTGCCGACAGGTCGTGGa < 1:2950907/69‑1 (MQ=255) tgaaGAACATCGTTACGCCCATCAGGATCGGCAGGATGTAGTACGGGTCCTGTGCCGACAGGTCGTGGa < 1:648443/69‑1 (MQ=255) cATCGTTACGCCCATCAGGATCGGCAGGATGTAGTACGGGTCCTGTGCCGACAGGTCGTGGATCcac < 1:1068274/67‑1 (MQ=255) cATCGTTACGCCCATCAGGATCGGCAGGATGTAGTACGGGTCCTGTGCCGACAGGTCGTGGATCcac < 1:3271105/67‑1 (MQ=255) cATCGTTACGCCCATCAGGATCGGCAGGATGTAGTACGGGTCCTGTGCCGACAGGTCGTGGATCcac < 1:3306336/67‑1 (MQ=255) aTCGTTACGCCCATCAGGATCGGCAGGATGTAGTACGGGTCCTGTGCCGACAGGTCGTGGATCcac < 1:1313184/66‑1 (MQ=255) aCGCCCATCAGGATCGGCAGGATGTAGTACGGGTCCTGTGCCGACAGGTCGTGGATCCACAGTGCAAAc > 1:1468191/1‑69 (MQ=255) aCGCCCATCAGGATCGGCAGGATGTAGTACGGGTCCTGTGCCGACAGGTCGTGGATCCACAGTGCAAAc > 1:199179/1‑69 (MQ=255) aCGCCCATCAGGATCGGCAGGATGTAGTACGGGTCCTGTGCCGACAGGTCGTGGATCCACAGTGCAAAc < 1:2292373/69‑1 (MQ=255) aCGCCCATCAGGATCGGCAGGATGTAGTACGGGTCCTGTGCCGACAGGTCGTGGATCCACAGTGCAAAc > 1:364074/1‑69 (MQ=255) aCGCCCATCAGGATCGGCAGGATGTAGTACGGGTCCTGTGCCGACAGGTCGTGGATCCACAGTGCAAAc < 1:1020553/69‑1 (MQ=255) aCGCCCATCAGGATCGGCAGGATGTAGTACGGGTCCTGTGCCGACAGGTCGTGGATCCACAGTGCAAAc > 1:734401/1‑69 (MQ=255) aCGCCCATCAGGATCGGCAGGATGTAGTACGGGTCCTGTGCCGACAGGTCGTGGATCCACAGTGCAAAc > 1:807228/1‑69 (MQ=255) cATCAGGATCGGCAGGATGTAGTACGGGTCCTGTGCAGACAGGTCGTGGATCCACAGTGCAAACGGTGc > 1:2363354/1‑69 (MQ=255) cATCAGGATCGGCAGGATGTAGTACGGGTCCTGTGCAGACAGGTCGTGGATCCACAGTGCAAACGGTGc > 1:1656009/1‑69 (MQ=255) cATCAGGATCGGCAGGATGTAGTACGGGTCCTGTGCAGACAGGTCGTGGATCCACAGTGCAAACGGTGc > 1:2301208/1‑69 (MQ=255) cATCAGGATCGGCAGGATGTAGTACGGGTCCTGTGCAGACAGGTCGTGGATCCACAGTGCAAACGGTGc > 1:2493985/1‑69 (MQ=255) cATCAGGATCGGCAGGATGTAGTACGGGTCCTGTGCAGACAGGTCGTGGATCCACAGTGCAAACGGTGc > 1:1291004/1‑69 (MQ=255) cATCAGGATCGGCAGGATGTAGTACGGGTCCTGTGCAGACAGGTCGTGGATCCACAGTGCAAACGGTGc > 1:1220624/1‑69 (MQ=255) cATCAGGATCGGCAGGATGTAGTACGGGTCCTGTGCAGACAGGTCGTGGATCCACAGTGCAAACGGTGc > 1:2531089/1‑69 (MQ=255) tCAGGATCGGCAGGATGTAGTACGGGTCCTGTGCCGACAGGTCGTGGATCcac < 1:1028559/53‑1 (MQ=255) tCAGGATCGGCAGGATGTAGTACGGGTCCTGTGCCGACAGGTCGTGGATCCACAGTGCAAACGGTGCCt > 1:1793997/1‑69 (MQ=255) tCGGCAGGATGTAGTACGGGTCCTGTGCAGACAGGTCGTGGATCCACAGTGCAAACGGTGCCTGACGCa < 1:2693118/69‑1 (MQ=255) tCGGCAGGATGTAGTACGGGTCCTGTGCAGACAGGTCGTGGATCCACAGTGCAAACGGTGCCTGACGCa < 1:904504/69‑1 (MQ=255) tCGGCAGGATGTAGTACGGGTCCTGTGCAGACAGGTCGTGGATCCACAGTGCAAACGGTGCCTGACGCa < 1:822820/69‑1 (MQ=255) tCGGCAGGATGTAGTACGGGTCCTGTGCAGACAGGTCGTGGATCCACAGTGCAAACGGTGCCTGACGCa < 1:2574834/69‑1 (MQ=255) tCGGCAGGATGTAGTACGGGTCCTGTGCAGACAGGTCGTGGATCCACAGTGCAAACGGTGCCTGACGCa < 1:1003589/69‑1 (MQ=255) tCGGCAGGATGTAGTACGGGTCCTGTGCAGACAGGTCGTGGATCCACAGTGCAAACGGTGCCTGACGCa < 1:624578/69‑1 (MQ=255) tCGGCAGGATGTAGTACGGGTCCTGTGCAGACAGGTCGTGGATCCACAGTGCAAACGGTGCCTGACGCa < 1:295587/69‑1 (MQ=255) tCGGCAGGATGTAGTACGGGTCCTGTGCAGACAGGTCGTGGATCCACAGTGCAAACGGTGCCTGACGCa < 1:493586/69‑1 (MQ=255) tCGGCAGGATGTAGTACGGGTCCTGTGCAGACAGGTCGTGGATCCACAGTGCAAACGGTGCCTGACGCa < 1:3047354/69‑1 (MQ=255) tCGGCAGGATGTAGTACGGGTCCTGTGCAGACAGGTCGTGGATCCACAGTGCAAACGGTGCCTGACGCa < 1:40060/69‑1 (MQ=255) tCGGCAGGATGTAGTACGGGTCCTGTGCAGACAGGTCGTGGATCCACAGTGCAAACGGTGCCTGACGCa < 1:3391429/69‑1 (MQ=255) tCGGCAGGATGTAGTACGGGTCCTGTGCAGACAGGTCGTGGATCCACAGTGCAAACGGTGCCTGACGCa < 1:235175/69‑1 (MQ=255) tCGGCAGGATGTAGTACGGGTCCTGTGCAGACAGGTCGTGGATCCACAGTGCAAACGGTGCCTGACGCa < 1:2235911/69‑1 (MQ=255) tCGGCAGGATGTAGTACGGGTCCTGTGCAGACAGGTCGTGGATCCACAGTGCAAACGGTGCCTGACGCa < 1:1062755/69‑1 (MQ=255) tCGGCAGGATGTAGTACGGGTCCTGTGCAGACAGGTCGTGGATCCACAGTGCAAACGGTGCCTGACGCa < 1:1436066/69‑1 (MQ=255) tCGGCAGGATGTAGTACGGGTCCTGTGCAGACAGGTCGTGGATCCACAGTGCAAACGGTGCCTGACGCa < 1:1304909/69‑1 (MQ=255) tCGGCAGGATGTAGTACGGGTCCTGTGCAGACAGGTCGTGGATCCACAGTGCAAACGGTGCCTGACGCa < 1:1220978/69‑1 (MQ=255) tCGGCAGGATGTAGTACGGGTCCTGTGCAGACAGGTCGTGGATCCACAGTGCAAACGGTGCCTGACGCa < 1:1735860/69‑1 (MQ=255) tCGGCAGGATGTAGTACGGGTCCTGTGCAGACAGGTCGTGGATCCACAGTGCAAACGGTGCCTGACGCa < 1:1241298/69‑1 (MQ=255) tCGGCAGGATGTAGTACGGGTCCTGTGCAGACAGGTCGTGGATCCACAGTGCAAACGGTGCCTGACGCa < 1:1240092/69‑1 (MQ=255) cGGCAGGATGTAGTACGGGTCCTGTGCAGACAGGTCGTg > 1:3424567/1‑39 (MQ=255) cGGCAGGATGTAGTACGGGTCCTGTGCAGACAGGTCGTg > 1:3344714/1‑39 (MQ=255) cGGCAGGATGTAGTACGGGTCCTGTGCAGACAGGTCGTg > 1:331383/1‑39 (MQ=255) cGGCAGGATGTAGTACGGGTCCTGTGCAGACAGGTCGTg > 1:166474/1‑39 (MQ=255) cGGCAGGATGTAGTACGGGTCCTGTGCAGACAGGTCGTg > 1:3194362/1‑39 (MQ=255) cGGCAGGATGTAGTACGGGTCCTGTGCAGACAGGTCGTg > 1:1800319/1‑39 (MQ=255) cGGCAGGATGTAGTACGGGTCCTGTGCAGACAGGTCGTg > 1:2955605/1‑39 (MQ=255) cGGCAGGATGTAGTACGGGTCCTGTGCAGACAGGTCGTg > 1:2845617/1‑39 (MQ=255) cGGCAGGATGTAGTACGGGTCCTGTGCAGACAGGTCGTg > 1:105476/1‑39 (MQ=255) cGGCAGGATGTAGTACGGGTCCTGTGCAGACAGGTCGTg > 1:649475/1‑39 (MQ=255) cGGCAGGATGTAGTACGGGTCCTGTGCAGACAGGTCGTg > 1:1996416/1‑39 (MQ=255) cAGGATGTAGTACGGGTCCTGTGCCGACAGGTCGTGGATCCACAGTGCAAACGGTGCCTGACTCAGTTc < 1:2352907/69‑1 (MQ=255) cAGGATGTAGTACGGGTCCTGTGCCGACAGGTCGTGGATCCACAGTGCAAACGGTGCCTGACGCAGTTc < 1:1294373/69‑1 (MQ=255) cAGGATGTAGTACGGGTCCTGTGCCGACAGGTCGTGGATCCACAGTGCAAACGGTGCCTGACGCAGTTc < 1:1691061/69‑1 (MQ=255) cAGGATGTAGTACGGGTCCTGTGCCGACAGGTCGTGGATCCACAGTGCAAACGGTGCCTGACGCAGTTc < 1:1179541/69‑1 (MQ=255) cAGGATGTAGTACGGGTCCTGTGCCGACAGGTCGTGGATCCACAGTGCAAACGGTGCCTGACGCAGTTc < 1:1971791/69‑1 (MQ=255) cAGGATGTAGTACGGGTCCTGTGCCGACAGGTCGTGGATCCACAGTGCAAACGGTGCCTGACGCAGTTc < 1:1278840/69‑1 (MQ=255) cAGGATGTAGTACGGGTCCTGTGCCGACAGGTCGTGGATCCACAGTGCAAACGGTGCCTGACGCAGTTc < 1:1986803/69‑1 (MQ=255) cAGGATGTAGTACGGGTCCTGTGCCGACAGGTCGTGGATCCACAGTGCAAACGGTGCCTGACGCAGTTc < 1:3118172/69‑1 (MQ=255) cAGGATGTAGTACGGGTCCTGTGCCGACAGGTCGTGGATCCACAGTGCAAACGGTGCCTGACGCAGTTc < 1:1766235/69‑1 (MQ=255) cAGGATGTAGTACGGGTCCTGTGCCGACAGGTCGTGGATCCACAGTGCAAACGGTGCCTGACGCAGTTc < 1:278702/69‑1 (MQ=255) aGGATGTAGTACGGGTCCTGTGCCGACAGGTCGTGGATCCACAGTGCAAACGGTGCCTGACGCAGTTc < 1:878024/68‑1 (MQ=255) tGTAGTACGGGTCCTGTGCAGACAGGTCGTGGATCCACAGTGCAAACGGTGCCTGACGCa < 1:3301278/60‑1 (MQ=255) ggTCCTGTGCCGACAGGTCGTGGATCCACAGTGCAAACGGTGCCTGACGCAGTTCAACGGAACCCATc > 1:1253739/1‑68 (MQ=255) ggTCCTGTGCCGACAGGTCGTGGATCCACAGTGCAAACGGTGCCTGACGCAGTTCAACGGAACCCATc > 1:1000049/1‑68 (MQ=255) ggTCCTGTGCCGACAGGTCGTGGATCCACAGTGCAAACGGTGCCTGACGCAGTTCAACGGAACCCATc > 1:1862961/1‑68 (MQ=255) ggTCCTGTGCCGACAGGTCGTGGATCCACAGTGCAAACGGTGCCTGACGCAGTTCAACGGAACCCATc > 1:2738099/1‑68 (MQ=255) gTCCTGTGCCGACAGGTCGTGGATCCACAGTGCAAACGGTGCCTGACGCAGTTc < 1:3252837/54‑1 (MQ=255) gTCCTGTGCAGACAGGTCGTGGATCCACAGTGCAAACGGTGCCTGACGCa < 1:1998421/50‑1 (MQ=255) ccGACAGGTCGTGGATCCACAGTGCAAACGGTGCCTGACGCAGTTCAACGGAACCCATCAGCAt > 1:12199/1‑64 (MQ=255) ccGACAGGTCGTGGATCCACAGTGCAAACGGTGCCTGACGCAGTTCAACGGAACCCATCAGCATgtag > 1:2409461/1‑68 (MQ=255) ccGACAGGTCGTGGATCCACAGTGCAAACGGTGCCTGACGCAGTTCAACGGAACCCATCAGCATgtag > 1:1616671/1‑68 (MQ=255) ccGACAGGTCGTGGATCCACAGTGCAAACGGTGCCTGACGCAGTTCAACGGAACCCATCAGCATgtag < 1:2414387/68‑1 (MQ=255) ccGACAGGTCGTGGATCCACAGTGCAAACGGTGCCTGACGCAGTTCAACGGAACCCATCAGCATgtag > 1:2426526/1‑68 (MQ=255) ccGACAGGTCGTGGATCCACAGTGCAAACGGTGCCTGACGCAGTTCAACGGAACCCATCAGCATgtag > 1:2637574/1‑68 (MQ=255) ccGACAGGTCGTGGATCCACAGTGCAAACGGTGCCTGACGCAGTTCAACGGAACCCATCAGCATgtag > 1:1222404/1‑68 (MQ=255) ccGACAGGTCGTGGATCCACAGTGCAAACGGTGCCTGACGCAGTTCAACGGAACCCATCAGCATgtag < 1:1834190/68‑1 (MQ=255) ccGACAGGTCGTGGATCCACAGTGCAAACGGTGCCTGACGCAGTTCAACGGAACCCATCAGCATgtag > 1:3174852/1‑68 (MQ=255) ccGACAGGTCGTGGATCCACAGTGCAAACGGTGCCTGACGCAGTTCAACGGAACCCATCAGCATgtag > 1:3244695/1‑68 (MQ=255) ccGACAGGTCGTGGATCCACAGTGCAAACGGTGCCTGACGCAGTTCAACGGAACCCATCAGCATgtag < 1:3249769/68‑1 (MQ=255) ccGACAGGTCGTGGATCCACAGTGCAAACGGTGCCTGACGCAGTTCAACGGAACCCATCAGCATgtag > 1:3269323/1‑68 (MQ=255) ccGACAGGTCGTGGATCCACAGTGCAAACGGTGCCTGACGCAGTTCAACGGAACCCATCAGCATgtag < 1:3388542/68‑1 (MQ=255) ccGACAGGTCGTGGATCCACAGTGCAAACGATGCCTGACGCAGTTCAACGGAACCCATCAGCATgta > 1:2716203/1‑67 (MQ=255) | GACATCTTCTGAATGAAGAACATCGTTACGCCCATCAGGATCGGCAGGATGTAGTACGGGTCCTGTGCCGACAGGTCGTGGATCCACAGTGCAAACGGTGCCTGACGCAGTTCAACGGAACCCATCAGCATGTAG > minE/2484803‑2484937 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |