| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,485,588 | G→A | 37.6% | G220G (GGC→GGT) | yidC ← | cytoplasmic insertase into membrane protein, Sec system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,485,588 | 0 | G | A | 37.6% | 59.9 / 60.0 | 98 | G220G (GGC→GGT) | yidC | cytoplasmic insertase into membrane protein, Sec system |
| Reads supporting (aligned to +/- strand): ref base G (14/47); new base A (27/10); total (41/57) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.86e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01 | |||||||||||
GGTATCGAACTTGTATTTCTCATACTTCTCGTCAGGCGTGGAGTACGCCGCGCCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTCCGGTATCGAGATGCGGTGGCAGAGTGATG > minE/2485537‑2485651 | ggTATCGAACTTGTATTTCTCATACTTCTCGTCAGGCGTGGAGTACGCCGCGCCACGGAAGGTGTGCAg < 1:195723/69‑1 (MQ=255) aTCGAACTTGTATTTCTCGTACTTCTCGTCAGGCGTGGAGTACGCCGCACCACGGAAGGTGTGCAGTg > 1:1347468/1‑68 (MQ=255) aTCGAACTTGTATTTCTCGTACTTCTCGTCAGGCGTGGAGTACGCCGCACCACGGAAGGTGTGCAGTg > 1:1342253/1‑68 (MQ=255) aTCGAACTTGTATTTCTCGTACTTCTCGTCAGGCGTGGAGTACGCCGCACCACGGAAGGTGTGCAGTg > 1:2276306/1‑68 (MQ=255) aTCGAACTTGTATTTCTCGTACTTCTCGTCAGGCGTGGAGTACGCCGCACCACGGAAGGTGTGCAGTg > 1:2739087/1‑68 (MQ=255) aTCGAACTTGTATTTCTCGTACTTCTCGTCAGGCGTGGAGTACGCCGCACCACGGAAGGTGTGCAGTg > 1:2976137/1‑68 (MQ=255) aTCGAACTTGTATTTCTCGTACTTCTCGTCAGGCGTGGAGTACGCCGCACCACGGAAGGTGTGCAGTg > 1:2193331/1‑68 (MQ=255) aTCGAACTTGTATTTCTCGTACTTCTCGTCAGGCGTGGAGTACGCCGCACCACGGAAGGTGTGCAGTg > 1:2034384/1‑68 (MQ=255) aTCGAACTTGTATTTCTCGTACTTCTCGTCAGGCGTGGAGTACGCCGCACCACGGAAGGTGTGCAGTg > 1:1889305/1‑68 (MQ=255) aTCGAACTTGTATTTCTCGTACTTCTCGTCAGGCGTGGAGTACGCCGCACCACGGAAGGTGTGCAGTg > 1:825421/1‑68 (MQ=255) aTCGAACTTGTATTTCTCGTACTTCTCGTCAGGCGTGGAGTACGCCGCACCACGGAAGGTGTGCAGTg > 1:1084689/1‑68 (MQ=255) aTCGAACTTGTATTTCTCGTACTTCTCGTCAGGCGTGGAGTACGCCGCACCACGGAAGGTGTGCAGTg > 1:851708/1‑68 (MQ=255) aTCGAACTTGTATTTCTCGTACTTCTCGTCAGGCGTGGAGTACGCCGCACCACGGAAGGTGTGCAGTg > 1:1058161/1‑68 (MQ=255) aTCGAACTTGTATTTCTCGTACTTCTCGTCAGGCGTGGAGTACGCCGCACCACGGAAGGTGTGCAGTg > 1:853311/1‑68 (MQ=255) aTCGAACTTGTATTTCTCGTACTTCTCGTCAGGCGTGGAGTACGCCGCACCACGGAAGGTGTGCAGTg > 1:1583119/1‑68 (MQ=255) aTCGAACTTGTATTTCTCGTACTTCTCGTCAGGCGTGGAGTACGCAGCACCACGGAAGGTGTGCAGt > 1:2640702/1‑67 (MQ=255) tCGAACTTGTATTTCTCATACTTCTCGTCAGGCGTGGAGTACGCCGCGCCACGGAAGGTGTGCAGTGCg < 1:95687/69‑1 (MQ=255) tCGAACTTGTATTTCTCATACTTCTCGTAAGGCGTGGAGTACGCCGCGCCACGGAAGGTGTGCAGTGCg < 1:3346525/69‑1 (MQ=255) gAACTTGTATTTCTCATACTTCTCGTCAGGCGTGGAGTACGCCGCGCCACGGAAGGTGTGCAGTGCGa < 1:252357/68‑1 (MQ=255) gAACTTGTATTTCTCATACTTCTCGTCAGGCGTGGAGTACGCCGCGCCACGGAAGGTGTGCAGTGCGa < 1:2313186/68‑1 (MQ=255) gAACTTGTATTTCTCATACTTCTCGTCAGGCGTGGAGTACGCCGCGCCACGGAAGGTGTGCAGTGCGa < 1:2716885/68‑1 (MQ=255) gAACTTGTATTTCTCATACTTCTCGTCAGGCGTGGAGTACGCCGCGCCACGGAAGGTGTGCAGTGCGa < 1:2230620/68‑1 (MQ=255) gAACTTGTATTTCTCATACTTCTCGTCAGGCGTGGAGTACGCCGCGCCACGGAAGGTGTGCAGTGCGa < 1:318536/68‑1 (MQ=255) gAACTTGTATTTCTCATACTTCTCGTCAGGCGTGGAGTACGCCGCGCCACGGAAGGTGTGCAGTGCGa < 1:3302029/68‑1 (MQ=255) gAACTTGTATTTCTCATACTTCTCGTCAGGCGTGGAGTACGCCGCGCCACGGAAGGTGTGCAGTGCGa < 1:608173/68‑1 (MQ=255) gAACTTGTATTTCTCATACTTCTCGTCAGGCGTGGAGTACGCCGCGCCACGGAAGGTGTGCAGTGCGa < 1:1036267/68‑1 (MQ=255) gAACTTGTATTTCTCATACTTCTCGTCAGGCGTGGAGTACGCCGCGCCACGGAAGGTGTGCAGTGCGa < 1:1630260/68‑1 (MQ=255) gAACTTGTATTTCTCATACTTCTCGTCAGGCGTGGAGTACGCCGCGCCACGGAAGGTGTGCAGTGCGa < 1:1406621/68‑1 (MQ=255) tGTATTTCTCGTACTTCTCGTCAGGCGTGGAGTACGCCGCACCACGGAAGGTGTGCAGTGCGAAGTtgc > 1:253073/1‑69 (MQ=255) tGTATTTCTCGTACTTCTCGTCAGGCGTGGAGTACGCCGCACCACGGAAGGTGTGCAGTGCGAAGTtgc > 1:3049335/1‑69 (MQ=255) tGTATTTCTCGTACTTCTCGTCAGGCGTGGAGTACGCCGCACCACGGAAGGTGTGCAGTGCGAAGTtgc > 1:3278155/1‑69 (MQ=255) tGTATTTCTCGTACTTCTCGTCAGGCGTGGAGTACGCCGCACCACGGAAGGTGTGCAGTGCGAAGTtgc > 1:852320/1‑69 (MQ=255) tGTATTTCTCGTACTTCTCGTCAGGCGTGGAGTACGCCGCACCACGGAAGGTGTGCAGTGCGAAGTtgc > 1:1503395/1‑69 (MQ=255) tGTATTTCTCGTACTTCTCGTCAGGCGTGGAGTACGCCGCACCACGGAAGGTGTGCAGTGCGAAGTtgc > 1:33654/1‑69 (MQ=255) tGTATTTCTCGTACTTCTCGTCAGGCGTGGAGTACGCCGCACCACGGAAGGTGTGCAGTGCGAAGTtgc > 1:798003/1‑69 (MQ=255) tGTATTTCTCGTACTTCTCGTCAGGCGTGGAGTACGCCGCACCACGGAAGGTGTGCAGTGCGAAGTtgc > 1:1121028/1‑69 (MQ=255) tGTATTTCTCGTACTTCTCGTCAGGCGTGGAGTACGCCGCACCACGGAAGGTGTGCAGTGCGAAGTtg > 1:2206610/1‑68 (MQ=255) tGTATTTCTCGTACTTCTCGTCAGGCGTGGAGTACGCCGCACCACGGAAGGTGTGCAGTGCGAAGTtg > 1:2734114/1‑68 (MQ=255) tGTATTTCTCGTACTTCTCGTCAGGCGTGGAGTACGCCGCACCACGGAAGGTGTGCAGTGCGAAGTtg > 1:470712/1‑68 (MQ=255) tGTATTTCTCGTACTTCTCGTCAGGCGTGGAGTACGCCGCACCACGGAAGGTGTGCAGTGCGAAGTtg > 1:1494616/1‑68 (MQ=255) tctcATACTTCTCGTCAGGCGTGGAGTACGCCGCGCCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTc > 1:2379307/1‑69 (MQ=255) tctcATACTTCTCGTCAGGCGTGGAGTACGCCGCGCCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTc > 1:1677448/1‑69 (MQ=255) tctcATACTTCTCGTCAGGCGTGGAGTACGCCGCGCCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTc > 1:1721891/1‑69 (MQ=255) tctcATACTTCTCGTCAGGCGTGGAGTACGCCGCGCCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTc > 1:2316345/1‑69 (MQ=255) tctcATACTTCTCGTCAGGCGTGGAGTACGCCGCGCCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTc > 1:857838/1‑69 (MQ=255) tctcATACTTCTCGTCAGGCGTGGAGTACGCCGCGCCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTc > 1:2242966/1‑69 (MQ=255) tctcATACTTCTCGTCAGGCGTGGAGTACGCCGCGCCACGGAAGGTGTGCAGTGCGAAGATGCTGCtt > 1:2482961/1‑68 (MQ=255) tctcGTCAGGCGTGGAGTACGCCGCGCCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTCCGGTATCga < 1:1058193/69‑1 (MQ=255) tctcGTCAGGCGTGGAGTACGCCGCGCCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTCCGGTATCga < 1:3321213/69‑1 (MQ=255) tctcGTCAGGCGTGGAGTACGCCGCGCCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTCCGGTATCga < 1:1100808/69‑1 (MQ=255) tctcGTCAGGCGTGGAGTACGCCGCGCCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTCCGGTATCga < 1:79355/69‑1 (MQ=255) tctcGTCAGGCGTGGAGTACGCCGCGCCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTCCGGTATCga < 1:12117/69‑1 (MQ=255) tctcGTCAGGCGTGGAGTACGCCGCGCCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTCCGGTATCga < 1:947471/69‑1 (MQ=255) tctcGTCAGGCGTGGAGTACGCCGCGCCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTCCGGTATCga < 1:1231220/69‑1 (MQ=255) tctcGTCAGGCGTGGAGTACGCCGCGCCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTCCGGTATCga < 1:2808495/69‑1 (MQ=255) tctcGTCAGGCGTGGAGTACGCCGCGCCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTCCGGTATCga < 1:1027142/69‑1 (MQ=255) tctcGTCAGGCGTGGAGTACGCCGCGCCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTCCGGTATCga < 1:2775808/69‑1 (MQ=255) tctcGTCAGGCGTGGAGTACGCCGCGCCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTCCGGTATCga < 1:1304129/69‑1 (MQ=255) tctcGTCAGGCGTGGAGTACGCCGCGCCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTCCGGTATCga < 1:484211/69‑1 (MQ=255) tctcGTCAGGCGTGGAGTACGCCGCGCCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTCCGGTATCga < 1:1418596/69‑1 (MQ=255) tctcGTCAGGCGTGGAGTACGCCGCGCCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTCCGGTATCga < 1:1737303/69‑1 (MQ=255) tctcGTCAGGCGTGGAGTACGCCGCGCCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTCCGGTATCga < 1:1739052/69‑1 (MQ=255) tctcGTCAGGCGTGGAGTACGCCGCGCCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTCCGGTATCga < 1:2072396/69‑1 (MQ=255) tctcGTCAGGCGTGGAGTACGCCGCGCCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTCCGGTATCga < 1:2139046/69‑1 (MQ=255) tctcGTCAGGCGTGGAGTACGCCGCGCCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTCCGGTATCga < 1:1462383/69‑1 (MQ=255) tctcGTCAGGCGTGGAGTACGCCGCGCCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTCCGGTATCga < 1:1367314/69‑1 (MQ=255) tctcGTCAGGCGTGGAGTACGCCGCGCCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTCCGGTATCga < 1:2654744/69‑1 (MQ=255) tctcGTCAGGCGTGGAGTACGCCGCGCCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTCCGGTATCga < 1:2532356/69‑1 (MQ=255) tctcGTCAGGCGTGGAGTACGCCGCGCCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTCCGGTATCga < 1:2431424/69‑1 (MQ=255) tctcGTCAGGCGTGGAGTACGCCGCGCCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTCCGGTATCga < 1:1341582/69‑1 (MQ=255) ctcGTCAGGCGTGGAGTACGCCGCGCCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTCCGGTATCgag > 1:2098056/1‑69 (MQ=255) ctcGTCAGGCGTGGAGTACGCCGCGCCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTCCGGTATCgag > 1:3402669/1‑69 (MQ=255) ctcGTCAGGCGTGGAGTACGCCGCGCCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTCCGGTATCgag > 1:3300325/1‑69 (MQ=255) ctcGTCAGGCGTGGAGTACGCCGCGCCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTCCGGTATCgag > 1:2399735/1‑69 (MQ=255) ctcGTCAGGCGTGGAGTACGCCGCGCCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTCCGGTATCgag > 1:3142708/1‑69 (MQ=255) gattACGCCGCACCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTCCGGTATCGAGATGCGGTGGCaga < 1:241180/66‑1 (MQ=255) gAGTACGCCGCACCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTCCGGTATCGAGATGCGGTGGCaga < 1:3299324/69‑1 (MQ=255) gAGTACGCCGCACCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTCCGGTATCGAGATGCGGTGGCaga < 1:954696/69‑1 (MQ=255) gAGTACGCCGCACCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTCCGGTATCGAGATGCGGTGGCaga < 1:2299977/69‑1 (MQ=255) gAGTACGCCGCACCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTCCGGTATCGAGATGCGGTGGCaga < 1:33302/69‑1 (MQ=255) gAGTACGCCGCACCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTCCGGTATCGAGATGCGGTGGCaga < 1:1168655/69‑1 (MQ=255) gAGTACGCCGCACCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTCCGGTATCGAGATGCGGTGGCaga < 1:1834854/69‑1 (MQ=255) gAGTACGCCGCACCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTCCGGTATCGAGATGCGGTGGCaga < 1:2103129/69‑1 (MQ=255) gAGTACGCCGCACCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTCCGGTATCGAGATGCGGTGGCaga < 1:1524546/69‑1 (MQ=255) gAGTACGCCGCACCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTCCGGTATCGAGATGCGGTGGCaga < 1:1963582/69‑1 (MQ=255) cgccgcGCCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTCCGGTATCGAGATGCGGTGGCAGAGTGa > 1:1052609/1‑68 (MQ=255) cgccgcGCCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTCCGGTATCGAGATGCGGTGGCAGAGTGa > 1:1847183/1‑68 (MQ=255) ccgcGCCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTCCGGTATCga < 1:413829/48‑1 (MQ=255) ccgcGCCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTCCGGTATCGAGATGCGGTGGCAGAGTGATg < 1:436061/68‑1 (MQ=255) ccgcGCCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTCCGGTATCGAGATGCGGTGGCAGAGTGATg < 1:2758290/68‑1 (MQ=255) ccgcGCCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTCCGGTATCGAGATGCGGTGGCAGAGTGATg < 1:689049/68‑1 (MQ=255) ccgcGCCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTCCGGTATCGAGATGCGGTGGCAGAGTGATg < 1:647067/68‑1 (MQ=255) ccgcGCCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTCCGGTATCGAGATGCGGTGGCAGAGTGATg < 1:1354594/68‑1 (MQ=255) ccgcGCCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTCCGGTATCGAGATGCGGTGGCAGAGTGATg < 1:392132/68‑1 (MQ=255) ccgcGCCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTCCGGTATCGAGATGCGGTGGCAGAGTGATg < 1:1316052/68‑1 (MQ=255) ccgcGCCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTCCGGTATCGAGATGCGGTGGCAGAGTGATg < 1:1172311/68‑1 (MQ=255) ccgcGCCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTCCGGTATCGAGATGCGGTGGCAGAGTGATg < 1:2967342/68‑1 (MQ=255) ccgcGCCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTCCGGTATCGAGATGCGGTGGCAGAGTGATg < 1:2984672/68‑1 (MQ=255) | GGTATCGAACTTGTATTTCTCATACTTCTCGTCAGGCGTGGAGTACGCCGCGCCACGGAAGGTGTGCAGTGCGAAGTTGCTGCTTCCGGTATCGAGATGCGGTGGCAGAGTGATG > minE/2485537‑2485651 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |