| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,485,674 | A→T | 17.5% | S192T (TCG→ACG) ‡ | yidC ← | cytoplasmic insertase into membrane protein, Sec system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,485,674 | 0 | A | T | 17.5% | 225.3 / 33.1 | 113 | S192T (TCG→ACG) ‡ | yidC | cytoplasmic insertase into membrane protein, Sec system |
| Reads supporting (aligned to +/- strand): ref base A (48/45); new base T (7/13); total (55/58) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 114 total aligned reads displayed.
GCTGCTTCCGGTATCGAGATGCGGTGGCAGAGTGATGGATTGCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGTTGTAGTTGACGTTGACAGCGTA > minE/2485615‑2485734 | gctgctTCCGGTATCGAGATGCGGTGGCAGAGTGATGGATTGCTTCAACTGACCAAAGGTGGAGAtt > 1:2003316/1‑67 (MQ=255)gctgctTCCGGTATCGAGATGCGGTGGCAGAGTGATGGATTGCTTCAACTGACCAAAGGTGGAGAtt > 1:1776584/1‑67 (MQ=255)gctgctTCCGGTATCGAGATGCGGTGGCAGAGTGATGGATTGCTTCAACTGACCAAAGGTGGAGAtt > 1:1628672/1‑67 (MQ=255) tCGAGATGCGGTGGCAGAGTGATGGATTGCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTtctc > 1:2701595/1‑68 (MQ=255) tCGAGATCCGGTGGCAGAGTGATGGATTGCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTtctc > 1:180431/1‑68 (MQ=255) gagaTGCGGTGGCAGAGTGATGGATTGCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGcc > 1:3114082/1‑69 (MQ=255) gagaTGCGGTGGCAGAGTGATGGATTGCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGcc > 1:1859009/1‑69 (MQ=255) gagaTGCGGTGGCAGAGTGATGGATTGCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGcc > 1:3353352/1‑69 (MQ=255) gagaTGCGGTGGCAGAGTGATGGATTGCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGcc > 1:3147639/1‑69 (MQ=255) gagaTGCGGTGGCAGAGTGATGGATTGCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGcc > 1:732514/1‑69 (MQ=255) gagaTGCGGTGGCAGAGTGATGGATTGCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGc > 1:288003/1‑68 (MQ=255) gagaTGCGGTGGCAGAGTGATGGATTGCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGc > 1:1345762/1‑68 (MQ=255) gagaTGCGGTGGCAGAGTGATGGATTGCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGc > 1:3019530/1‑68 (MQ=255) aTGCGGTGGCAGAGTGATGGATTGCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAg > 1:522373/1‑68 (MQ=255) aTGCGGTGGCAGAGTGATGGATTGCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGc > 1:2559267/1‑69 (MQ=255) aTGCGGTGGCAGAGTGATGGATTGCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGc > 1:3396427/1‑69 (MQ=255) aTGCGGTGGCAGAGTGATGGATTGCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGc > 1:2230534/1‑69 (MQ=255) aTGCGGTGGCAGAGTGATGGATTGCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGc > 1:623282/1‑69 (MQ=255) gCGGTGGCAGAGTGATGGATTGCTTCAACTGACCAAACGAGGAGATTTcc < 1:565879/50‑1 (MQ=255) gCGGTGGCAGAGTGATGGATTGCTTCAACTGACCAAACGAGGAGATTTcc < 1:1846582/50‑1 (MQ=255) gCGGTGGCAGAGTGATGGATTGCTTCAACTGACCAAACGAGGAGATTTcc < 1:3012156/50‑1 (MQ=255) gCGGTGGCAGAGTGATGGATTGCTTCAACTGACCAAACGAGGAGATTTcc < 1:1695937/50‑1 (MQ=255) gCGGTGGCAGAGTGATGGATTGCTTCAACTGACCAAACGAGGAGATTTcc < 1:2165570/50‑1 (MQ=255) gCGGTGGCAGAGTGATGGATTGCTTCAACTGACCAAACGAGGAGATTTcc < 1:2069188/50‑1 (MQ=255) gCGGTGGCAGAGTGATGGATTGCTTCAACTGACCAAACGAGGAGATTTcc < 1:620832/50‑1 (MQ=255) gCGGTGGCAGAGTGATGGATTGCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGCGt < 1:178034/69‑1 (MQ=255) gCGGTGGCAGAGTGATGGATTGCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGCGt < 1:2243591/69‑1 (MQ=255) gCGGTGGCAGAGTGATGGATTGCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGCGt < 1:2282532/69‑1 (MQ=255) gCGGTGGCAGAGTGATGGATTGCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGCGt < 1:1062960/69‑1 (MQ=255) gCGGTGGCAGAGTGATGGATTGCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGCGt < 1:362398/69‑1 (MQ=255) gCGGTGGCAGAGTGATGGATTGCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGCGt < 1:2316142/69‑1 (MQ=255) cGGTGGCAGAGTGATGGATTGCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGCGtt < 1:2273039/69‑1 (MQ=255) cGGTGGCAGAGTGATGGATTGCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGCGtt < 1:794734/69‑1 (MQ=255) agagTGATGGATTGCTTCAACTGACCAAAGGTGGAGATTTCCGGCg > 1:549505/1‑46 (MQ=255) agagTGATGGATTGCTTCAACTGACCAAAGGTGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCAc > 1:1869480/1‑68 (MQ=255) agagTGATGGATTGCTTCAACTGACCAAAGGTGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACg > 1:1863269/1‑69 (MQ=255) gTGATGGATTGCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGt > 1:19994/1‑67 (MQ=255) gTGATGGATTGCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGt > 1:3208421/1‑67 (MQ=255) gTGATGGATTGCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGt > 1:2941719/1‑67 (MQ=255) gTGATGGATTGCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGt > 1:2938195/1‑67 (MQ=255) gTGATGGATTGCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGt > 1:585082/1‑67 (MQ=255) gTGATGGATTGCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGt > 1:3331625/1‑67 (MQ=255) gTGATGGATTGCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGt > 1:1294191/1‑67 (MQ=255) gATGGATTGCTTCTACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCTGCGTTCTGCACGTTGTa < 1:1627918/69‑1 (MQ=255) gATGGATTGCTTCAACTGACCAAAGGTGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGTTGTa < 1:3133929/69‑1 (MQ=255) gATGGATTGCTTCAACTGACCAAAGGTGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGTTGTa < 1:3104844/69‑1 (MQ=255) gATGGATTGCTTCAACTGACCAAAGGTGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGTTGTa < 1:1061567/69‑1 (MQ=255) gATGGATTGCTTCAACTGACCAAAGGTGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGTTGTa < 1:2608909/69‑1 (MQ=255) gATGGATTGCTTCAACTGACCAAAGGTGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGTTGTa < 1:1345180/69‑1 (MQ=255) gATGGATTGCTTCAACTGACCAAAGGTGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGTTGTa < 1:244539/69‑1 (MQ=255) gATGGATTGCTTCAACTGACCAAAGGTGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGTTGTa < 1:1961399/69‑1 (MQ=255) gATGGATTGCTTCAACTGACCAAAGGTGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGTTGTa < 1:513212/69‑1 (MQ=255) gATGGATTGCTTCAACTGACCAAAGGTGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGTTGTa < 1:229701/69‑1 (MQ=255) gATGGATTGCTTCAACTGACCAAAGGTGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGTTGTa < 1:2377246/69‑1 (MQ=255) gATGGATTGCTTCAACTGACCAAAGGTGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGTTGTa < 1:1461552/69‑1 (MQ=255) gATGGATTGCTTCAACTGACCAAAGGTGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGTTGTa < 1:2475479/69‑1 (MQ=255) gATGGATTGCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGTTGTa < 1:3402843/69‑1 (MQ=255) gATGGATTGCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGTTGTa < 1:1160764/69‑1 (MQ=255) gATGGATTGCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGTTGTa < 1:1106280/69‑1 (MQ=255) gATGGATTGCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGTTGTa < 1:3264489/69‑1 (MQ=255) gATGGATTGCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGTTGTa < 1:1852559/69‑1 (MQ=255) gATGGATTGCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGTTGTa < 1:382321/69‑1 (MQ=255) gATGGATTGCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGTTGTa < 1:2966609/69‑1 (MQ=255) gATGGATTGCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGTTGTa < 1:537395/69‑1 (MQ=255) gATGGAATGCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGTTGTa < 1:661073/69‑1 (MQ=255) aTGGATTGCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGTTGt > 1:3056402/1‑67 (MQ=255) aTGGATTGCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGTTGTAg > 1:3316739/1‑69 (MQ=255) aTGGATTGCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGTTGTAg > 1:677007/1‑69 (MQ=255) aTGGATTGCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGTTGTAg > 1:2436163/1‑69 (MQ=255) aTGGATTGCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGTTGTAg > 1:3007732/1‑69 (MQ=255) aTGGATTGCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGTTGTAg > 1:275362/1‑69 (MQ=255) aTGGATTGCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGTTGTAg > 1:1037846/1‑69 (MQ=255) aTGGATTGCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGTTGTAg > 1:1339033/1‑69 (MQ=255) aTGGATTGCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGTTGTAg > 1:930968/1‑69 (MQ=255) aTGGATTGCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGTTGTAg > 1:2304671/1‑69 (MQ=255) aTGGATTGCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGTTGTAg > 1:2245465/1‑69 (MQ=255) aTGGATTGCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGTTGTAg > 1:1892561/1‑69 (MQ=255) aTGGATTGCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGTTGTAg > 1:1913605/1‑69 (MQ=255) ttGCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGTTGTAgttgac > 1:1035684/1‑69 (MQ=255) ttGCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGTTGTAgttgac > 1:1074775/1‑69 (MQ=255) ttGCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGTTGTAgttgac > 1:1986660/1‑69 (MQ=255) gCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTtc > 1:2200526/1‑38 (MQ=255) gCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCATGTTGTAgttgagaa > 1:295003/1‑66 (MQ=255) gCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGTTGTAgttgagaa > 1:1152541/1‑66 (MQ=255) gCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGTTGTAgttgagaa > 1:2592541/1‑66 (MQ=255) gCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGTTGTAgttgagaa > 1:668228/1‑66 (MQ=255) gCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGTTGTAgttgagaa > 1:736301/1‑66 (MQ=255) gCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGTTGTAgttgagaa > 1:1002396/1‑66 (MQ=255) ttCAACTGATCAAACGAGGAGATTTCCAGGGGTTTCTCGCCAGCGTTCTGCACGTTGTAgttgacgtt < 1:924256/68‑1 (MQ=255) ttCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGCGt < 1:2887374/46‑1 (MQ=255) ttCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGTTGTAgttgacgtt < 1:814410/68‑1 (MQ=255) ttCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGTTGTAgttgacgtt < 1:963769/68‑1 (MQ=255) ttCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGTTGTAgttgacgtt < 1:97425/68‑1 (MQ=255) ttCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGTTGTAgttgacgtt < 1:818243/68‑1 (MQ=255) ttCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGTTGTAgttgacgtt < 1:487089/68‑1 (MQ=255) ttCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGTTGTAgttgacgtt < 1:1030172/68‑1 (MQ=255) tCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGTTGTAgttgacgtt < 1:1886051/67‑1 (MQ=255) cAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGTTGTAGTTGACGTTGACAGCGTa < 1:2676677/67‑1 (MQ=255) cAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGTTGTAGTTGACGTTGACAGCGTa < 1:2333703/67‑1 (MQ=255) gaggagATTTCCAGCTGTTTCTCGCCAGCGTTCTGCACGTTGTa < 1:1578834/44‑1 (MQ=255) | GCTGCTTCCGGTATCGAGATGCGGTGGCAGAGTGATGGATTGCTTCAACTGACCAAACGAGGAGATTTCCAGCGGTTTCTCGCCAGCGTTCTGCACGTTGTAGTTGACGTTGACAGCGTA > minE/2485615‑2485734 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |