| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,489,649 | A→G | 19.5% | V216V (GTA→GTG) | dnaN → | DNA polymerase III, beta subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,489,649 | 0 | A | G | 19.5% | 78.7 / 11.6 | 56 | V216V (GTA→GTG) | dnaN | DNA polymerase III, beta subunit |
| Reads supporting (aligned to +/- strand): ref base A (20/25); new base G (6/5); total (26/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
GTACCGCGTAAAGGCGTGATTGAACTGATGCGTATGCTCGACGGCGGCGACAATCCGCTGCGCGTACAGATTGGCAGCAACAACATTCGCGCCCACGTTGGCGACTTTATCTTCACCTCCAAACTGG > minE/2489584‑2489710 | gTACCGCGTAAAGGCGTGATTGAACTGATGCGTATGCTCGACGGCGGCGACAATCCGCTGCGCGTACAg < 1:2232767/69‑1 (MQ=255)gTACCGCGTAAAGGCGTGATTGAACTGATGCGTATGCTCGACGGCGGCGACAATCCGCTGCGCGTACAg < 1:2606458/69‑1 (MQ=255)gTACCGCGTAAAGGCGTGATTGAACTGATGCGTATGCTCGACGGCGGCGACAATCCGCTGCGCGTACAg < 1:2805602/69‑1 (MQ=255) aaaGGCGTGATTGAACTGATGCGTATGCTCGACGGCGGCGACAATCCGCTGCGCGTACAGATTGgcagc < 1:108596/69‑1 (MQ=255) aaaGGCGTGATTGAACTGATGCGTATGCTCGACGGCGGCGACAATCCGCTGCGCGTACAGATTGgcagc < 1:1994873/69‑1 (MQ=255) cGTGATTGAACTGATGCGTATGCTCGACGGCGGCGACAATCCGCTGCGCGTACAGATTGGCAGcaacaa < 1:3153467/69‑1 (MQ=255) cGTGATTGAACTGATGCGTATGCTCGACGGCGGCGACAATCCGCTGCGCGTACAGATTGGCAGcaacaa < 1:2828996/69‑1 (MQ=255) cGTGATTGAACTGATGCGTATGCTCGACGGCGGCGACAATCCGCTGCGCGTACAGATTGGCAGcaacaa < 1:1067775/69‑1 (MQ=255) tGATTGAACTGATGCGTATGCTCGACGGCGGCGACAATCCGCTGCGCGTACAGATTGGCAGCAacaaca < 1:458354/69‑1 (MQ=255) tGATTGAACTGATGCGTATGCTCGACGGCGGCGACAATCCGCTGCGCGTACAGATTGGCAGCAacaaca < 1:1518548/69‑1 (MQ=255) tGATTGAACTGATGCGTATGCTCGACGGCGGCGACAATCCGCTGCGCGTACAGATTGGCAGCAacaaca < 1:3388830/69‑1 (MQ=255) tGATTGAACTGATGCGTATGCTCGACGGCGGCGACAATCCGCTGCGCGTACAGATTGGCAGCAacaaca < 1:3045169/69‑1 (MQ=255) tGATTGAACTGATGCGTATGCTCGACGGCGGCGACAATCCGCTGCGCGTACAGATTGGCAGCAacaaca < 1:672418/69‑1 (MQ=255) tGATTGAACTGATGCGTATGCTCGACGGCGGCGACAATCCGCTGCGCGTACAGATTGGCAGCAacaaca < 1:1233385/69‑1 (MQ=255) tGATTGAACTGATGCGTATGCTCGACGGCGGCGACAATCCGCTGCGCGTACAGATTGGCAGCAacaaca < 1:2496430/69‑1 (MQ=255) gATTGAACTGATGCGTATGCTCGACGGCGGCGACAATCCGCTGCGCGTACAGATTGGCAGCAacaaca < 1:1471497/68‑1 (MQ=255) aTTGAACTGATGCGTATGCTCGACGGCGGCGACAATCCGCTGCGCGTACa < 1:1072963/50‑1 (MQ=255) aTTGAACTGATGCGTATGCTCGACGGCGGCGACAATCCGCTGCGCGTACa < 1:3000410/50‑1 (MQ=255) aTTGAACTGATGCGTATGCTCGACGGCGGCGACAATCCGCTGCGCGTACa < 1:268377/50‑1 (MQ=255) aTTGAACTGATGCGTATGCTCGACGGCGGCGACAATCCGCTGCGCGTACa < 1:2362881/50‑1 (MQ=255) aTTGAACTGATGCGTATGCTCGACGGCGGCGACAATCCGCTGCGCGTACa < 1:80891/50‑1 (MQ=255) aTTGAACTGATGCGTATGCTCGACGGCGGCGACAATCCGCTGCGCGTACa < 1:1550051/50‑1 (MQ=255) aTTGAACTGATGCGTATGCTCGACGGCGGCGACAATCCGCTGCGCGTACa < 1:2028181/50‑1 (MQ=255) aTTGAACTGATGCGTATGCTCGACGGCGGCGACAATCCGCTGCGCGTACa < 1:1828437/50‑1 (MQ=255) aTTGAACTGATGCGTATGCTCGACGGCGGCGACAATCCGCTGCGCGTACa < 1:1839206/50‑1 (MQ=255) aTTGAACTGATGCGTATGCTCGACGGCGGCGACAATCCGCTGCGCGTACAGa > 1:1218649/1‑52 (MQ=255) aTTGAACTGATGCGTATGCTCGACGGCGGCGACAATCCGCTGCGCGTACAGATTGgcagc > 1:1620466/1‑60 (MQ=255) aTTGAACTGATGCGTATGCTCGACGGCGGCGACAATCCGCTGCGCGTACAGATTGgcagc > 1:982975/1‑60 (MQ=255) aTTGAACTGATGCGTATGCTCGACGGCGGCGACAATCCGCTGCGCGTACAGATTGgcagc > 1:515413/1‑60 (MQ=255) aTTGAACTGATGCGTATGCTCGACGGCGGCGACAATCCGCTGCGCGTACAGATTGgcagc > 1:460440/1‑60 (MQ=255) aTTGAACTGATGCGTATGCTCGACGGCGGCGACAATCCGCTGCGCGTACAGATTGgcagc > 1:1488450/1‑60 (MQ=255) aTTGAACTGATGCGTATGCTCGACGGCGGCGACAATCCGCTGCGCGTACAGATTGgcagc > 1:381760/1‑60 (MQ=255) aTTGAACTGATGCGTATGCTCGACGGCGGCGACAATCCGCTGCGCGTACAGATTGgcagc > 1:1527405/1‑60 (MQ=255) aTTGAACTGATGCGTATGCTCGACGGCGGCGACAATCCGCTGCGCGTACAGATTGgcagc > 1:2885546/1‑60 (MQ=255) aTTGAACTGATGCGTATGCTCGACGGCGGCGACAATCCGCTGCGCGTACAGATTGgcagc > 1:1899123/1‑60 (MQ=255) aTTGAACTGATGCGTATGCTCGACGGCGGCGACAATCCGCTGCGCGTACAGATTGgcagc > 1:2020122/1‑60 (MQ=255) aTTGAACTGATGCGTATGCTCGACGGCGGCGACAATCCGCTGCGCGTACAGATTGgcagc > 1:280482/1‑60 (MQ=255) aTTGAACTGATGCGTATGCTCGACGGCGGCGACAATCCGCTGCGCGTACAGATTGgcagc > 1:2071597/1‑60 (MQ=255) aTTGAACTGATGCGTATGCTCGACGGCGGCGACAATCCGCTGCGCGTACAGATTGgcagc > 1:2339445/1‑60 (MQ=255) tGAACTGATGCGTATGCTCGACGGCGGCGACAATCCGCTGCGCGTACAGATTGGCAGCAACAACATTcg > 1:3071738/1‑69 (MQ=255) cTCGACGGCGGCGACAATCCGCTGCGCGTACAGATTgg > 1:2902899/1‑38 (MQ=255) cTCGACGGCGGCGACAATCCGCTGCGCGTACAGATTGGCAGCAACAACATTCGCGCCCACGTTGGCGAc > 1:1890864/1‑69 (MQ=255) cTCGACGGCGGCGACAATCCGCTGCGCGTACAGATTGGCAGCAACAACATTCGCGCCCACGTTGGCGAc > 1:1419952/1‑69 (MQ=255) ccGCTGCGCGTACAGATTGGCAGCAACAACATTCGCGCCCACGTTGGCGACTTTATCTTCACCTCCaaa > 1:3119777/1‑69 (MQ=255) ccGCTGCGCGTACAGATTGGCAGCAACAACATTCGCGCCCACGTTGGCGACTTTATCTTCACCTCCaaa > 1:2533158/1‑69 (MQ=255) gCTGCGCGTGCAGATTGGCAGCAACAACATTCGCGCCCACGTTGGCGACTTTATCTTCACCTCGAAAc > 1:1910857/1‑68 (MQ=255) gCTGCGCGTGCAGATTGGCAGCAACAACATTCGCGCCCACGTTGGCGACTTTATCTTCACCTCGAAAc > 1:1919422/1‑68 (MQ=255) gCTGCGCGTGCAGATTGGCAGCAACAACATTCGCGCCCACGTTGGCGACTTTATCTTCACCTCGAAACt > 1:3114784/1‑69 (MQ=255) gCTGCGCGTGCAGATTGGCAGCAACAACATTCGCGCCCACGTTGGCGACTTTATCTTCACCTCGAAACt > 1:386519/1‑69 (MQ=255) gCTGCGCGTGCAGATTGGCAGCAACAACATTCGCGCCCACGTTGGCGACTTTATCTTCACCTCGAAACt > 1:2315356/1‑69 (MQ=255) gCTGCGCGTGCAGATTGGCAGCAACAACATTCGCGCCCACGTTGGCGACTTTATCTTCACCTCGAAACt > 1:487520/1‑69 (MQ=255) tGCGCGTGCAGATTGGCAGCAACAACATTCGCGCCCACGTTGGCGACTTTATCTTCACCTCGAAACtgg < 1:2057545/69‑1 (MQ=255) tGCGCGTGCAGATTGGCAGCAACAACATTCGCGCCCACGTTGGCGACTTTATCTTCACCTCGAAACtgg < 1:3157873/69‑1 (MQ=255) tGCGCGTGCAGATTGGCAGCAACAACATTCGCGCCCACGTTGGCGACTTTATCTTCACCTCGAAACtgg < 1:3098715/69‑1 (MQ=255) tGCGCGTGCAGATTGGCAGCAACAACATTCGCGCCCACGTTGGCGACTTTATCTTCACCTCGAAACtgg < 1:1599602/69‑1 (MQ=255) gcgcGTGCAGATTGGCAGCAACAACATTCGCGCCCACGTTGGCGACTTTATCTTCACCTCGAAACtgg < 1:2234021/68‑1 (MQ=255) | GTACCGCGTAAAGGCGTGATTGAACTGATGCGTATGCTCGACGGCGGCGACAATCCGCTGCGCGTACAGATTGGCAGCAACAACATTCGCGCCCACGTTGGCGACTTTATCTTCACCTCCAAACTGG > minE/2489584‑2489710 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |