| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,489,703 | C→G | 26.6% | S234S (TCC→TCG) | dnaN → | DNA polymerase III, beta subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,489,703 | 0 | C | G | 26.6% | 64.7 / 21.8 | 45 | S234S (TCC→TCG) | dnaN | DNA polymerase III, beta subunit |
| Reads supporting (aligned to +/- strand): ref base C (26/7); new base G (6/6); total (32/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.57e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01 | |||||||||||
CCGCTGCGCGTACAGATTGGCAGCAACAACATTCGCGCCCACGTTGGCGACTTTATCTTCACCTCCAAACTGGTGGATGGTCGCTTCCCGGATTATCGCCGCGTTCTGCCGAAGAACCCGGACAAACATC > minE/2489638‑2489767 | ccGCTGCGCGTACAGATTGGCAGCAACAACATTCGCGCCCACGTTGGCGACTTTATCTTCACCTCCaaa > 1:2533158/1‑69 (MQ=255)ccGCTGCGCGTACAGATTGGCAGCAACAACATTCGCGCCCACGTTGGCGACTTTATCTTCACCTCCaaa > 1:3119777/1‑69 (MQ=255) gCTGCGCGTGCAGATTGGCAGCAACAACATTCGCGCCCACGTTGGCGACTTTATCTTCACCTCGAAAc > 1:1910857/1‑68 (MQ=255) gCTGCGCGTGCAGATTGGCAGCAACAACATTCGCGCCCACGTTGGCGACTTTATCTTCACCTCGAAAc > 1:1919422/1‑68 (MQ=255) gCTGCGCGTGCAGATTGGCAGCAACAACATTCGCGCCCACGTTGGCGACTTTATCTTCACCTCGAAACt > 1:2315356/1‑69 (MQ=255) gCTGCGCGTGCAGATTGGCAGCAACAACATTCGCGCCCACGTTGGCGACTTTATCTTCACCTCGAAACt > 1:487520/1‑69 (MQ=255) gCTGCGCGTGCAGATTGGCAGCAACAACATTCGCGCCCACGTTGGCGACTTTATCTTCACCTCGAAACt > 1:3114784/1‑69 (MQ=255) gCTGCGCGTGCAGATTGGCAGCAACAACATTCGCGCCCACGTTGGCGACTTTATCTTCACCTCGAAACt > 1:386519/1‑69 (MQ=255) tGCGCGTGCAGATTGGCAGCAACAACATTCGCGCCCACGTTGGCGACTTTATCTTCACCTCGAAACtgg < 1:1599602/69‑1 (MQ=255) tGCGCGTGCAGATTGGCAGCAACAACATTCGCGCCCACGTTGGCGACTTTATCTTCACCTCGAAACtgg < 1:2057545/69‑1 (MQ=255) tGCGCGTGCAGATTGGCAGCAACAACATTCGCGCCCACGTTGGCGACTTTATCTTCACCTCGAAACtgg < 1:3157873/69‑1 (MQ=255) tGCGCGTGCAGATTGGCAGCAACAACATTCGCGCCCACGTTGGCGACTTTATCTTCACCTCGAAACtgg < 1:3098715/69‑1 (MQ=255) gcgcGTGCAGATTGGCAGCAACAACATTCGCGCCCACGTTGGCGACTTTATCTTCACCTCGAAACtgg < 1:2234021/68‑1 (MQ=255) acaacaTTCGCGCCCACGTTGGCGACTTTATCTTCACCTCGAAACtgg < 1:1363656/48‑1 (MQ=255) aacaTTCGCGCCCACGTTGGCGACTTTATCTTCACCTCCAAACTGGTGGATGGTCGCTTCCCGGATTAt > 1:1371542/1‑69 (MQ=255) aacaTTCGCGCCCACGTTGGCGACTTTATCTTCACCTCCAAACTGGTGGATGGTCGCTTCCCGGATTAt > 1:1915529/1‑69 (MQ=255) aacaTTCGCGCCCACGTTGGCGACTTTATCTTCACCTCCAAACTGGTGGATGGTCGCTTCCCGGATTAt > 1:2148251/1‑69 (MQ=255) cgcgCCCACGTTGGCGACTTTATCTTCACCTCCAAACTGGTGGATGGTCGCTTCCCg > 1:2876741/1‑57 (MQ=255) cgcgCCCACGTTGGCGACTTTATCTTCACCTCCAAACTGGTGGATGGTCGCTTCCCg > 1:482303/1‑57 (MQ=255) cgcgCCCACGTTGGCGACTTTATCTTCACCTCCAAACTGGTGGATGGTCGCTTCCCg > 1:762657/1‑57 (MQ=255) cgcgCCCACGTTGGCGACTTTATCTTCACCTCCAAACTGGTGGATGGTCGCTTCCCGGATTATcgccgc > 1:1543351/1‑69 (MQ=255) cgcgCCCACGTTGGCGACTTTATCTTCACCTCCAAACTGGTGGATGGTCGCTTCCCGGATTATcgccgc > 1:2325158/1‑69 (MQ=255) cgcgCCCACGTTGGCGACTTTATCTTCACCTCCAAACTGGTGGATGGTCGCTTCCCGGATTATcgccgc > 1:2988931/1‑69 (MQ=255) cgcgCCCACGTTGGCGACTTTATCTTCACCTCCAAACTGGTGGATGGTCGCTTCCCGGATTATcgccgc > 1:946363/1‑69 (MQ=255) cgcgCCCACGTAGGCGACTTTATCTTCACCTCCaa > 1:2477124/1‑35 (MQ=255) ggCGACTTTATCTTCACCTCCAAACTGGTGGATGGTCGCTTCCCg > 1:1051656/1‑45 (MQ=255) ggCGACTTTATCTTCACCTCCAAACTGGTGGATGGTCGCTTCCCGGATTATCGCCGCGTTCTGCCgaag > 1:3052631/1‑69 (MQ=255) ggCGACTTTATCTTCACCTCCAAACTGGTGGATGGTCGCTTCCCGGATTATCGCCGCGTTCTGCCgaag > 1:853053/1‑69 (MQ=255) ggCGACTTTATCTTCACCTCCAAACTGGTGGATGGTCGCTTCCCGGATTATCGCCGCGTTCTGCCgaag > 1:1673945/1‑69 (MQ=255) ggCGACTTTATCTTCACCTCCAAACTGGTGGATGGTCGCTTCCCGGATTATCGCCGCGTTCTGCCgaag > 1:440666/1‑69 (MQ=255) ggCGACTTTATCTTCACCTCCAAACTGGTGGATGGTCGCTTCCCGGAATATcgc > 1:2188388/1‑54 (MQ=255) tATCTTCACCTCCAAACTGGTGGATGGTCGCTTCCCGGATTATCGCCGCGTTCTGCCGAAGAACCCgg > 1:2179300/1‑68 (MQ=255) tATCTTCACCTCCAAACTGGTGGATGGTCGCTTCCCGGATTATCGCCGCGTTCTGCCGAAGAACCCgg > 1:346152/1‑68 (MQ=255) aTCTTCACCTCCAAACTGGTGGATGGTCGCTTCCCGGATTATCGCCGCGTTCTGCCTAAGAACCCGGa > 1:1998384/1‑68 (MQ=255) aTCTTCACCTCCAAACTGGTGGATGGTCGCTTCCCGGATTATCGCCGCGTTCTGCCGAAGAACCCGGa > 1:2909612/1‑68 (MQ=255) aTCTTCACCTCCAAACTGGTGGATGGTCGCTTCCCGGATTATCGCCGCGTTCTGCCGAAGAACCCGGa > 1:935356/1‑68 (MQ=255) aTCTTCACCTCCAAACTGGTGGATGGTCGCTTCCCGGATTATCGCCGCGTTCTGCCGAAGAACCCGGa > 1:3206577/1‑68 (MQ=255) aTCTTCACCTCCAAACTGGTGGATGGTCGCTTCCCGGATTATCGCCGCGTTCTGCCGAAGAACCCGGa > 1:2444721/1‑68 (MQ=255) tCCAAACTGGTGGATGGTCGCTTCCCGGATTATCGCCGCGTTCTGCCGAAGAACCCGGACAAACATc < 1:2307380/67‑1 (MQ=255) tCCAAACTGGTGGATGGTCGCTTCCCGGATTATCGCCGCGTTCTGCCGAAGAACCCGGACAAACATc < 1:1701820/67‑1 (MQ=255) tCCAAACTGGTGGATGGTCGCTTCCCGGATTATCGCCGCGTTCTGCCGAAGAACCCGGACAAACATc < 1:1324137/67‑1 (MQ=255) tCCAAACTGGTGGATGGTCGCTTCCCGGATTATCGCCGCGTTCTGCCGAAGAACCCGGACAAACATc < 1:814059/67‑1 (MQ=255) tCCAAACTGGTGGATGGTCGCTTCCCGGATTATCGCCGCGTTCTGCCGAAGAACCCGGACAAACATc < 1:308392/67‑1 (MQ=255) tCCAAACTGGTGGATGGTCGCTTCCCGGATTATCGCCGCGTTCTGCCGAAGAACCCGGACAAACATc < 1:2737662/67‑1 (MQ=255) tCCAAACTGGTGGATGGTCGCTTCCCGGATTATCGCCGCGTTCTGCCGAAGAACCCGGACAAACATc < 1:1191271/67‑1 (MQ=255) | CCGCTGCGCGTACAGATTGGCAGCAACAACATTCGCGCCCACGTTGGCGACTTTATCTTCACCTCCAAACTGGTGGATGGTCGCTTCCCGGATTATCGCCGCGTTCTGCCGAAGAACCCGGACAAACATC > minE/2489638‑2489767 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |