| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,492,469 | G→T | 100% | L422L (CTG→CTT) | gyrB → | DNA gyrase, subunit B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,492,469 | 0 | G | T | 83.9% | 55.6 / 5.6 | 31 | L422L (CTG→CTT) | gyrB | DNA gyrase, subunit B |
| Reads supporting (aligned to +/- strand): ref base G (5/0); new base T (16/10); total (21/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.47e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.44e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CTGCCGGGCAAACTGGCAGACTGCCAGGAACGCGATCCGGCGCTTTCCGAACTGTACCTGGTGGAAGGGGACTCCGCGGGCGGCTCTGCGAAGCAGGGGCGTAAC > minE/2492410‑2492514 | cTGCCGGGCAAACTGGCAGACTGCCAGGAACGCGATCCGGCGCTTTCCGAACTGTACCTTGTGGAAggg > 1:1132391/1‑69 (MQ=255)cTGCCGGGCAAACTGGCAGACTGCCAGGAACGCGATCCGGCGCTTTCCGAACTGTACCTTGTGGAAggg > 1:240695/1‑69 (MQ=255)cTGCCGGGCAAACTGGCAGACTGCCAGGAACGCGATCCGGCGCTTTCCGAACTGTACCTTGTGGAAggg > 1:2498280/1‑69 (MQ=255)cTGCCGGGCAAACTGGCAGACTGCCAGGAACGCGATCCGGCGCTTTCCGAACTGTACCTTGTGGAAggg > 1:2027634/1‑69 (MQ=255)cTGCCGGGCAAACTGGCAGACTGCCAGGAACGCGATCCGGCGCTTTCCGAACTGTACCTTGTGGAAggg > 1:1817490/1‑69 (MQ=255)cTGCCGGGCAAACTGGCAGACTGCCAGGAACGCGATCCGGCGCTTTCCGAACTGTACCTTGTGGAAggg > 1:3161098/1‑69 (MQ=255)cTGCCGGGCAAACTGGCAGACTGCCAGGAACGCGATCCGGCGCTTTCCGAACTGTACCTTGTGGAAggg > 1:1385621/1‑69 (MQ=255)cTGCCGGGCAAACTGGCAGACTGCCAGGAACGCGATCCGGCGCTTTCCGAACTGTACCTTGTGGAAggg > 1:3165201/1‑69 (MQ=255)cTGCCGGGCAAACTGGCAGACTGCCAGGAACGCGATCCGGCGCTTTCCGAACTGTACCTTGTGGAAggg > 1:1313171/1‑69 (MQ=255)cTGCCGGGCAAACTGGCAGACTGCCAGGAACGCGATCCGGCGCTTTCCGAACTGTACCTTGTGGAAggg > 1:392851/1‑69 (MQ=255)cTGCCGGGCAAACTGGCAGACTGCCAGGAACGCGATCCGGCGCTTTCCGAACTGTACCTTGTGGAAggg > 1:552728/1‑69 (MQ=255)cTGCCGGGCAAACTGGCAGACTGCCAGGAACGCGATCCGGCGCTTTCCGAACTGTACCTTGTGGAAggg > 1:1245456/1‑69 (MQ=255)cTGCCGGGCAAACTGGCAGACTGCCAGGAACGCGATCCGGCGCTTTCCGAACTGTACCTTGTGGAAggg > 1:1231757/1‑69 (MQ=255)cTGCCGGGCAAACTGGCAGACTGCCAGGAACGCGATCCGGCGCTTTCCGAACTGTACCTTGTGGAAggg > 1:1226560/1‑69 (MQ=255)cTGCCGGGCAAACTGGCAGACTGCCAGGAACGCGATCCGGCGCTTTCCGAACTGTACCTTGTGGAAggg > 1:116165/1‑69 (MQ=255)cTGCCGGGCAAACTGGCAGACTGCCAGGAACGCGATCCGGCGCTTTCCGAACTGTACCTTGTGGAAgg > 1:2218235/1‑68 (MQ=255) cTGCCAGGAACGCGATCCGGCGCTTTCCGAACTGTACCtggtg < 1:2505807/43‑1 (MQ=255) cTGCCAGGAACGCGATCCGGCGCTTTCCGAACTGTACCtggtg < 1:2503701/43‑1 (MQ=255) cTGCCAGGAACGCGATCCGGCGCTTTCCGAACTGTACCtggtg < 1:717213/43‑1 (MQ=255) cTGCCAGGAACGCGATCCGGCGCTTTCCGAACTGTACCtggtg < 1:2076242/43‑1 (MQ=255) cTGCCAGGAACGCGATCCGGCGCTTTCCGAACTGTACCtggtg < 1:121533/43‑1 (MQ=255) cTGCCAGGAACGCGATCCGGCGCTTTCCGAACTGTACCtggtg < 1:1764037/43‑1 (MQ=255) aaCGCGATCCGGCGCTTTCCGAACTGTACCTGGTGGAAGGGGACTCCGCGGGCGGCTCTGCGAAGCAgg > 1:2930123/1‑69 (MQ=255) aaCGCGATCCGGCGCTTTCCGAACTGTACCTGGTGGAAGGGGACTCCGCGGGCGGCTCTGCGAAGCAgg > 1:1366793/1‑69 (MQ=255) cgATCCGGCGCTTTCCGAACTGTACCTTGTGGAAgg < 1:2047451/36‑1 (MQ=255) cgATCCGGCGCTTTCCGAACTGTACCTTGTGGAAgg < 1:1265933/36‑1 (MQ=255) cgATCCGGCGCTTTCCGAACTGTACCTTGTGGAAgg < 1:3115450/36‑1 (MQ=255) cgATCCGGCGCTTTCCGAACTGTACCTTGTGGAAgg < 1:3105990/36‑1 (MQ=255) cgATCCGGCGCTTTCCGAACTGTACCTTGTGGAAgg < 1:2954555/36‑1 (MQ=255) cgATCCGGCGCTTTCCGAACTGTACCTTGTGGAAgg < 1:1918552/36‑1 (MQ=255) cgATCCGGCGCTTTCCGAACTGTACCTTGTGGAAgg < 1:1074935/36‑1 (MQ=255) cgATCCGGCGCTTTCCGAACTGTACCTTGTCGAAgg < 1:143417/36‑1 (MQ=37) gATCCGGCGCTTTCCGAACTGTACCTTGTGGAAgg < 1:3360573/35‑1 (MQ=255) gATCCGGCGCTTTCCGAACTGTACCTTGTGGAAgg < 1:78433/35‑1 (MQ=255) ccGGCGCTTTCCGAACTGTACCTGGTGGAAGGGGACTCCGCGGGCGGCTCTGCGAAGCAGGGGCGTaa > 1:305218/1‑68 (MQ=255) ccGGCGCTTTCCGAACTGTACCTGGTGGAAGGGGACTCCGCGGGCGGCTCTGCGAAGCAGGGGCGTaa > 1:1310254/1‑68 (MQ=255) ccGGCGCTTTCCGAACTGTACCTGGTGGAAGGGGACTCCGCGGGCGGCTCTGCGAAGCAGGGGCGTAAc > 1:798102/1‑69 (MQ=255) | CTGCCGGGCAAACTGGCAGACTGCCAGGAACGCGATCCGGCGCTTTCCGAACTGTACCTGGTGGAAGGGGACTCCGCGGGCGGCTCTGCGAAGCAGGGGCGTAAC > minE/2492410‑2492514 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |