| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,495,805 | A→G | 46.7% | A173A (GCT→GCC) | emrD ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,495,805 | 0 | A | G | 46.7% | 9.6 / 91.3 | 105 | A173A (GCT→GCC) | emrD | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base A (42/14); new base G (29/20); total (71/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.76e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01 | |||||||||||
Only 100 of 105 total aligned reads displayed.
GGCGCGTGCGCGGTGCATCGACCGGACGCGTTTCCGGCATCCAGCGGGCCATACTGAAGGTCACACCAGCACAAAGAACCAACAAAAAGAGATAACAGGCGCGCCAGTTCCACATCGTATCCAGCAGACCGCCG > minE/2495738‑2495871 | ggCGCGTGCGCGGTGCATCGACCGGACGCGTTTCCGGCATCCAGCGGGCCATACTGAAGGTCACACCAg < 1:5132/69‑1 (MQ=255)ggCGCGTGCGCGGTGCATCGACCGGACGCGTTTCCGGCATCCAGCGGGCCATACTGAAGGTCACACCAg < 1:222689/69‑1 (MQ=255) tGCATCGACCGGACGCGTTTCCGGCATCCAGCGGGCCATACTGAAGGTCACACCAGCACAAAGAACca > 1:306041/1‑68 (MQ=255) cGGACGCGTTTCCGGCATCCAGCGGTCCATACTGAAGGTCACACCAGCACAAAGAACCAACAAAAagag < 1:666969/69‑1 (MQ=255) cGGACGCGTTTCCGGCATCCAGCGGGCCATACTGAAGGTCACACCAGCACAAAGAACCAACAAAAagag < 1:3015757/69‑1 (MQ=255) ggACGCGTTTCCGGCATCCAGCGGGCCATACTGAAGGTCACACCAGCACAAAGAACCAACAAAAagag < 1:1929263/68‑1 (MQ=255) gCATCCAGCGGGCCATACTGAAGGTCACACCAGCACAAAGAACCAACAAAAAGAGATAACAGGCGCGcc < 1:3226441/69‑1 (MQ=255) gCATCCAGCGGGCCATACTGAAGGTCACACCAGCACAAAGAACCAACAAAAAGAGATAACAGGCGCGcc < 1:963020/69‑1 (MQ=255) gCATCCAGCGGGCCATACTGAAGGTCACACCAGCACAAAGAACCAACAAAAAGAGATAACAGGCGCGcc < 1:3222478/69‑1 (MQ=255) aTCCAGCGGGCCATACTGAAGGTCACACCAGCACAAAGAACCAACAAAAAGAGATAACAGGCGCGCCAg < 1:2747729/69‑1 (MQ=255) aTCCAGCGGGCCATACTGAAGGTCACACCAGCACAAAGAACCAACAAAAAGAGATAACAGGCGCGCCAg < 1:156088/69‑1 (MQ=255) aTCCAGCGGGCCATACTGAAGGTCACACCAGCACAAAGAACCAACAAAAAGAGATAACAGGCGCGCCAg < 1:2052741/69‑1 (MQ=255) cAGCGGGCCATACTGAAGGTCACACCAGCACAAAGAACCAACAAAAAGAGAt > 1:2130457/1‑52 (MQ=255) cAGCGGGCCATACTGAAGGTCACACCAGCACAAAGAACCAACAAAAAGAGAt > 1:2193301/1‑52 (MQ=255) cAGCGGGCCATACTGAAGGTCACACCAGCACAAAGAACCAACAAAAAGAGAt > 1:2343101/1‑52 (MQ=255) cAGCGGGCCATACTGAAGGTCACACCAGCACAAAGAACCAACAAAAAGAGAt > 1:2632045/1‑52 (MQ=255) cAGCGGGCCATACTGAAGGTCACACCAGCACAAAGAACCAACAAAAAGAGAt > 1:2668477/1‑52 (MQ=255) cAGCGGGCCATACTGAAGGTCACACCAGCACAAAGAACCAACAAAAAGAGAt > 1:278711/1‑52 (MQ=255) cAGCGGGCCATACTGAAGGTCACACCAGCACAAAGAACCAACAAAAAGAGAt > 1:1777501/1‑52 (MQ=255) cAGCGGGCCATACTGAAGGTCACACCAGCACAAAGAACCAACAAAAAGAGAt > 1:1658166/1‑52 (MQ=255) cAGCGGGCCATACTGAAGGTCACACCAGCACAAAGAACCAACAAAAAGAGAt > 1:1431967/1‑52 (MQ=255) cAGCGGGCCATACTGAAGGTCACACCAGCACAAAGAACCAACAAAAAGAGAt > 1:1224803/1‑52 (MQ=255) cAGCGGGCCATACTGAAGGTCACACCAGCACAAAGAACCAACAAAAAGAGATAACAGGCGCGCCAGtt > 1:2388689/1‑68 (MQ=255) cAGCGGGCCATACTGAAGGTCACACCAGCACAAAGAACCAACAAAAAGAGATAACAGGCGCGCCAGTTc > 1:3121353/1‑69 (MQ=255) cAGCGGGCCATACTGAAGGTCACACCAGCACAAAGAACCAACAAAAAGAGATAACAGGCGCGCCAGTTc > 1:3058501/1‑69 (MQ=255) cAGCGGGCCATACTGAAGGTCACACCAGCACAAAGAACCAACAAAAAGAGATAACAGGCGCGCCAGTTc > 1:2299709/1‑69 (MQ=255) cAGCGGGCCATACTGAAGGTCACACCAGCACAAAGAACCAACAAAAAGAGATAACAGGCGCGCCAGTTc > 1:1334713/1‑69 (MQ=255) cAGCGGGCCATACTGAAGGTCACACCAGCACAAAGAACCAACAAAAAGAGATAACAGGCGCGCCAGTTc > 1:460342/1‑69 (MQ=255) cAGCGGGCCATACTGAAGGTCACACCAGCACAAAGAACCAACAAAAAGAGATAACAGGCGCGCCAGTTc > 1:1525424/1‑69 (MQ=255) cAGCGGGCCATACTGAAGGTCACACCAGCACAAAGAACCAACAAAAAGAGATAACAGGCGCGCCAGTTc > 1:2112938/1‑69 (MQ=255) cAGCGGGCCATACTGAAGGTCACACCAGCACAAAGAACCAACAAAAAGAGATAACAGGCGCGCCAGTTc > 1:3042678/1‑69 (MQ=255) cAGCGGGCCATACTGAAGGTCACACCAGCACAAAGAACCAACAAAAAGAGATAACAGGCGCGCCAGTTc > 1:1534756/1‑69 (MQ=255) cAGCGGGCCATACTGAAGGTCACACCAGCACAAAGAACCAACAAAAAGAGATAACAGGCGCGCCAGTTc > 1:499243/1‑69 (MQ=255) cAGCGGGCCATACTGAAGGTCACACCAGCACAAAGAACCAACAAAAAGAGATAACAGGCGCGCCAGTTc > 1:1573857/1‑69 (MQ=255) cAGCGGGCCATACTGAAGGTCACACCAGCACAAAGAACCAACAAAAAGAGATAACAGGCGCGCCAGTTc > 1:1899884/1‑69 (MQ=255) cAGCGGGCCATACTGAAGGTCACACCAGCACAAAGAACCAACAAAAAGAGATAACAGGCGCGCCAGTTc > 1:2374508/1‑69 (MQ=255) cAGCGGGCCATACTGAAGGTCACACCAGCACAAAGAACCAACAAAAAGAGATAACAGGCGCGCCAGTTc > 1:3289840/1‑69 (MQ=255) cAGCGGGCCATACTGAAGGTCACACCAGCACAAAGAACCAACAAAAAGAGATAACAGGCGCGCCAGTTc > 1:957537/1‑69 (MQ=255) gCGGGCCATACTGAAGGTCACACCAGCACAAAGAACCAACAAAAAGAGATAACAGGCGCGCCAGTTCca > 1:3087124/1‑69 (MQ=255) gCGGGCCATACTGAAGGTCACACCAGCACAAAGAACCAACAAAAAGAGATAACAGGCGCGCCAGTTCca > 1:2878614/1‑69 (MQ=255) gCGGGCCATACTGAAGGTCACACCAGCACAAAGAACCAACAAAAAGAGATAACAGGCGCGCCAGTTCca > 1:809541/1‑69 (MQ=255) gCGGGCCATACTGAAGGTCACACCAGCACAAAGAACCAACAAAAAGAGATAACAGGCGCGCCAGTTCca > 1:1722535/1‑69 (MQ=255) gCGGGCCATACTGAAGGTCACACCAGCACAAAGAACCAACAAAAAGAGATAACAGGCGCGCCAGTTCca > 1:3352675/1‑69 (MQ=255) ccATACTGAAGGTCACACCAGCACAAAGAACCAACAAAAAGAGATAACAGGCGCGCCAGTTCCACATCg > 1:2079763/1‑69 (MQ=255) aTACTGAAAGTCACACCGGCACAAAGAACCAACAAAAAGAGATAACAGGCACGCCAGTTCCACATCg > 1:1555726/1‑67 (MQ=255) aTACTGAAAGTCACACCGGCACAAAGAACCAACAAAAAGAGATAACAGGCACGCCAGTTCCACATCGt > 1:9663/1‑68 (MQ=255) aTACTGAAAGTCACACCGGCACAAAGAACCAACAAAAAGAGATAACAGGCACGCCAGTTCCACATCGTa > 1:2912159/1‑69 (MQ=255) aTACTGAAAGTCACACCGGCACAAAGAACCAACAAAAAGAGATAACAGGCACGCCAGTTCCACATCGTa > 1:1085166/1‑69 (MQ=255) aTACTGAAAGTCACACCGGCACAAAGAACCAACAAAAAGAGATAACAGGCACGCCAGTTCCACATCGTa > 1:2846673/1‑69 (MQ=255) aTACTGAAAGTCACACCGGCACAAAGAACCAACAAAAAGAGATAACAGGCACGCCAGTTCCACATCGTa > 1:2995633/1‑69 (MQ=255) aTACTGAAAGTCACACCGGCACAAAGAACCAACAAAAAGAGATAACAGGCACGCCAGTTCCACATCGTa > 1:3010094/1‑69 (MQ=255) aTACTGAAAGTCACACCGGCACAAAGAACCAACAAAAAGAGATAACAGGCACGCCAGTTCCACATCGTa > 1:3065942/1‑69 (MQ=255) aTACTGAAAGTCACACCGGCACAAAGAACCAACAAAAAGAGATAACAGGCACGCCAGTTCCACATCGTa > 1:3178733/1‑69 (MQ=255) aTACTGAAAGTCACACCGGCACAAAGAACCAACAAAAAGAGATAACAGGCACGCCAGTTCCACATCGTa > 1:448314/1‑69 (MQ=255) aTACTGAAAGTCACACCGGCACAAAGAACCAACAAAAAGAGATAACAGGCACGCCAGTTCCACATCGTa > 1:3269060/1‑69 (MQ=255) aTACTGAAAGTCACACCGGCACAAAGAACCAACAAAAAGAGATAACAGGCACGCCAGTTCCACATCGTa > 1:435823/1‑69 (MQ=255) aTACTGAAAGTCACACCGGCACAAAGAACCAACAAAAAGAGATAACAGGCACGCCAGTTCCACATCGTa > 1:3346340/1‑69 (MQ=255) aTACTGAAAGTCACACCGGCACAAAGAACCAACAAAAAGAGATAACAGGCACGCCAGTTCCACATCGTa > 1:3347539/1‑69 (MQ=255) aTACTGAAAGTCACACCGGCACAAAGAACCAACAAAAAGAGATAACAGGCACGCCAGTTCCACATCGTa > 1:2657431/1‑69 (MQ=255) aTACTGAAAGTCACACCGGCACAAAGAACCAACAAAAAGAGATAACAGGCACGCCAGTTCCACATCGTa > 1:1068872/1‑69 (MQ=255) aTACTGAAAGTCACACCGGCACAAAGAACCAACAAAAAGAGATAACAGGCACGCCAGTTCCACATCGTa > 1:1178878/1‑69 (MQ=255) aTACTGAAAGTCACACCGGCACAAAGAACCAACAAAAAGAGATAACAGGCACGCCAGTTCCACATCGTa > 1:1490561/1‑69 (MQ=255) aTACTGAAAGTCACACCGGCACAAAGAACCAACAAAAAGAGATAACAGGCACGCCAGTTCCACATCGTa > 1:155532/1‑69 (MQ=255) aTACTGAAAGTCACACCGGCACAAAGAACCAACAAAAAGAGATAACAGGCACGCCAGTTCCACATCGTa > 1:1593942/1‑69 (MQ=255) aTACTGAAAGTCACACCGGCACAAAGAACCAACAAAAAGAGATAACAGGCACGCCAGTTCCACATCGTa > 1:1629266/1‑69 (MQ=255) aTACTGAAAGTCACACCGGCACAAAGAACCAACAAAAAGAGATAACAGGCACGCCAGTTCCACATCGTa > 1:1697568/1‑69 (MQ=255) aTACTGAAAGTCACACCGGCACAAAGAACCAACAAAAAGAGATAACAGGCACGCCAGTTCCACATCGTa > 1:1792592/1‑69 (MQ=255) aTACTGAAAGTCACACCGGCACAAAGAACCAACAAAAAGAGATAACAGGCACGCCAGTTCCACATCGTa > 1:1950237/1‑69 (MQ=255) aTACTGAAAGTCACACCGGCACAAAGAACCAACAAAAAGAGATAACAGGCACGCCAGTTCCACATCGTa > 1:2098681/1‑69 (MQ=255) aTACTGAAAGTCACACCGGCACAAAGAACCAACAAAAAGAGATAACAGGCACGCCAGTTCCACATCGTa > 1:2323394/1‑69 (MQ=255) aTACTGAAAGTCACACCGGCACAAAGAACCAACAAAAAGAGATAACAGGCACGCCAGTTCCACATCGTa > 1:2333141/1‑69 (MQ=255) aTACTGAAAGTCACACCGGCACAAAGAACCAACAAAAAGAGATAACAGGCACGCCAGTTCCACATCGTa > 1:2437442/1‑69 (MQ=255) aCTGAAAGTCACACCGGCACAAAGAACCAACAAAAAGAGATGAc < 1:2880041/44‑1 (MQ=37) aCTGAAAGTCACACCGGCACAAAGAACCAACAAAAAGAGATAAc < 1:2567216/44‑1 (MQ=38) aCTGAAAGTCACACCGGCACAAAGAACCAACAAAAAGAGATAAc < 1:2815050/44‑1 (MQ=38) aCTGAAAGTCACACCGGCACAAAGAACCAACAAAAAGAGATAAc < 1:1177856/44‑1 (MQ=38) aCTGAAAGTCACACCGGCACAAAGAACCAACAAAAAGAGATAAc < 1:1178601/44‑1 (MQ=38) aCTGAAAGTCACACCGGCACAAAGAACCAACAAAAAGAGATAAc < 1:2877012/44‑1 (MQ=38) aCTGAAAGTCACACCGGCACAAAGAACCAACAAAAAGAGATAAc < 1:473862/44‑1 (MQ=38) aCTGAAAGTCACACCGGCACAAAGAACCAACAAAAAGAGATAAc < 1:262165/44‑1 (MQ=38) aCTGAAAGTCACACCGGCACAAAGAACCAACAAAAAGAGATAAc < 1:449111/44‑1 (MQ=38) aCTGAAAGTCACACCGGCACAAAGAACCAACAAAAAGAGATAAc < 1:399925/44‑1 (MQ=38) aCTGAAAGTCACACCGGCACAAAGAACCAACAAAAAGAGATAAc < 1:3389413/44‑1 (MQ=38) aCTGAAAGTCACACCGGCACAAAGAACCAACAAAAAGAGATAAc < 1:2316075/44‑1 (MQ=38) aCTGAAAGTCACACCGGCACAAAGAACCAACAAAAAGAGATAAc < 1:1667151/44‑1 (MQ=38) aCTGAAAGTCACACCGGCACAAAGAACCAACAAAAAGAGATAAc < 1:1862474/44‑1 (MQ=38) aCTGAAAGTCACACCGGCACAAAGAACCAACAAAAAGAGATAAc < 1:3018771/44‑1 (MQ=38) aCTGAAAGTCACACCGGCACAAAGAACCAACAAAAAGAGATAAc < 1:3195899/44‑1 (MQ=38) aCTGAAAGTCACACCGGCACAAAGAACCAACAAAAAGAGATAAc < 1:1962366/44‑1 (MQ=38) aCTGAAAGTCACACCGGCACAAAGAACCAACAAAAAGAGATAAc < 1:1983385/44‑1 (MQ=38) aCTGAAAGTCACACCGGCACAAAGAACCAACAAAAAGAGATAAc < 1:3113728/44‑1 (MQ=38) aCTGAAAGTCACACCGGCACAAAGAACCAACAAAAAGAGATAAc < 1:2233629/44‑1 (MQ=38) cTGAAGGTCACACCAGCACAAAGAACCAACAAAAAGAGATAACAGGCGCGCCAGTTCCACATCGTATcc > 1:1990015/1‑69 (MQ=255) cacaCCAGCACAAAGAACCAACAAAAAGAGATAACAGGCGCGcc < 1:1183929/44‑1 (MQ=255) cacaCCAGCACAAAGAACCAACAAAAAGAGATAACAGGCGCGCCAGTTCCACATCGTATCCAGCAGAcc > 1:3154017/1‑69 (MQ=255) ccAGCACAAAGAACCAACAAAAAGAGATAACAGGCGCGCCAGTTCCACATCGTATCCAGCAGAccgccg > 1:1920469/1‑69 (MQ=255) ccAGCACAAAGAACCAACAAAAAGAGATAACAGGCGCGCCAGTTCCACATCGTATCCAGCAGAccgccg > 1:2599431/1‑69 (MQ=255) ccAGCACAAAGAACCAACAAAAAGAGATAACAGGCGCGCCAGTTCCACATCGTATCCAGCAGAccgccg > 1:1198538/1‑69 (MQ=255) ccAGCACAAAGAACCAACAAAAAGAGATAACAGGCGCGCCAGTTCCACATCGTATCCAGCAGAccgccg > 1:951705/1‑69 (MQ=255) ccAGCACAAAGAACCAACAAAAAGAGATAACAGGCGCGCCAGTTCCACATCGTATCCAGCAGAccgccg > 1:1092748/1‑69 (MQ=255) | GGCGCGTGCGCGGTGCATCGACCGGACGCGTTTCCGGCATCCAGCGGGCCATACTGAAGGTCACACCAGCACAAAGAACCAACAAAAAGAGATAACAGGCGCGCCAGTTCCACATCGTATCCAGCAGACCGCCG > minE/2495738‑2495871 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |