Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,498,633 T→G 34.3% V391V (GTT→GTG ilvB → acetolactate synthase I, large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,498,6330TG34.3% 53.0 / 49.2 67V391V (GTT→GTGilvBacetolactate synthase I, large subunit
Reads supporting (aligned to +/- strand):  ref base T (25/19);  new base G (7/16);  total (32/35)
Fisher's exact test for biased strand distribution p-value = 7.03e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGGCCTGATCAACGCCGTTGCCGCCTGTGTCGATGACAATGCAATTATCACCACCGACGTTGGTCAGCATCAGATGTGGACCGCGCAAGCTTATCCGCTCAATCGCCCACGCCAGTGGCTGACCTCCGG  >  minE/2498572‑2498701
                                                             |                                                                    
aCGGCCTGATCAACGCCGTTGCCGCCTGTGTCGATGACAATGCAATTATCACCACCGACGTTg                                                                     >  1:3127527/1‑63 (MQ=255)
aCGGCCTGATCAACGCCGTTGCCGCCTGTGTCGATGACAATGCAATTATCACCACCGACGTTg                                                                     >  1:198969/1‑63 (MQ=255)
aCGGCCTGATCAACGCCGTTGCCGCCTGTGTCGATGACAATGCAATTATCACCACCGACGTTg                                                                     >  1:1690332/1‑63 (MQ=255)
aCGGCCTGATCAACGCCGTTGCCGCCTGTGTCGATGACAATGCAATTATCACCACCGACGTTg                                                                     >  1:1416763/1‑63 (MQ=255)
aCGGCCTGATCAACGCCGTTGCCGCCTGTGTCGATGACAATGCAATTATCACCACCGACGTTg                                                                     >  1:3418462/1‑63 (MQ=255)
     cTGATCAACGCCGTTGCCGCCTGTGTCGATGACAATGCAATTATCACCACCGACGTTGGTCAGCATCa                                                           >  1:2533154/1‑68 (MQ=255)
     cTGATCAACGCCGTTGCCGCCTGTGTCGATGACAATGCAATTATCACCACCGACGTTGGTCAGCATCAg                                                          >  1:2475401/1‑69 (MQ=255)
          cAACGCCGTTGCCGCCTGTGTCGATGACAATGCAATTATCACCACCGACGTTGGTCAGCATCAGAtgtg                                                     <  1:426780/69‑1 (MQ=255)
          cAACGCCGTTGCCGCCTGTGTCGATGACAATGCAATTATCACCACCGACGTTGGTCAGCATCAGAtg                                                       <  1:2639264/67‑1 (MQ=255)
          cAACGCCGTTGCCGCCTGTGTCGATGACAATGCAATTATCACCACCGACGTTGGTCAGCATCAGAtg                                                       <  1:2994658/67‑1 (MQ=255)
          cAACGCCGTTGCCGCCTGTGTCGATGACAATGCAATTATCACCACCGACGTTGGTCAGCATCAGAtg                                                       <  1:1934816/67‑1 (MQ=255)
          cAACGCCGTTGCCGCCTGTGTCGATGACAATGCAATTATCACCACCGACGTTGGTCAGCATCAGAtg                                                       <  1:2136006/67‑1 (MQ=255)
          cAACGCCGTTGCCGCCTGTGTCGATGACAATGCAATTATCACCACCGACGTTGGTCAGCATCAGAtg                                                       <  1:2939234/67‑1 (MQ=255)
                   tGCCGCCTGTGTCGATGACAATGCAATTATCACCACCGACGTTGGTCAGCATCAGATGTGGACCGCGCa                                            <  1:3373606/69‑1 (MQ=255)
                   tGCCGCCTGTGTCGATGACAATGCAATTATCACCACCGACGTTGGTCAGCATCAGATGTGGACCGCGCa                                            <  1:1726679/69‑1 (MQ=255)
                         cTGTGTCGATGACAATGCAATTATCACCACCGACGTTGGTCAGCATCAGATGTGGACCGCGCAAGCTTa                                      <  1:429952/69‑1 (MQ=255)
                         cTGTGTCGATGACAATGCAATTATCACCACCGACGTTGGTCAGCATCAGATGTGGACCGCGCAAGCTTa                                      <  1:3342673/69‑1 (MQ=255)
                         cTGTGTCGATGACAATGCAATTATCACCACCGACGTTGGTCAGCATCAGATGTGGACCGCGCAAGCTTa                                      >  1:323637/1‑69 (MQ=255)
                         cTGTGTCGATGACAATGCAATTATCACCACCGACGTTGGTCAGCATCAGATGTGGACCGCGCAAGCTTa                                      >  1:1141094/1‑69 (MQ=255)
                         cTGTGTCGATGACAATGCAATTATCACCACCGACGTTGGTCAGCATCAGATGTGGACCGCGCAAGCTTa                                      <  1:2076421/69‑1 (MQ=255)
                           gtgtCGATGACAATGCGATTATCACCACCGACGTGGGTCAGCATCAGATGTGGACCGCGCAAGCCTATc                                    >  1:498126/1‑69 (MQ=255)
                           gtgtCGATGACAATGCGATTATCACCACCGACGTGGGTCAGCATCAGATGTGGACCGCGCAAGCCTATc                                    >  1:1488751/1‑69 (MQ=255)
                           gtgtCGATGACAATGCGATTATCACCACCGACGTGGGTCAGCATCAGATGTGGACCGCGCAAGCCTATc                                    >  1:2763560/1‑69 (MQ=255)
                           gtgtCGATGACAATGCGATTATCACCACCGACGTGGGTCAGCATCAGATGTGGACCGCGCAAGCCTATc                                    >  1:275021/1‑69 (MQ=255)
                           gtgtCGATGACAATGCGATTATCACCACCGACGTGGGTCAGCATCAGATGTGGACCGCGCAAGCCTATc                                    >  1:1180360/1‑69 (MQ=255)
                           gtgtCGATGACAATGCGATTATCACCACCGACGTGGGTCAGCATCAGATGTGGACCGCGCAAGCCTATc                                    >  1:297135/1‑69 (MQ=255)
                           gtgtCGATGACAATGCGATTATCACCACCGACGTGGGTCAGCATCAGATGTGGACCGCGCAAGCCTATc                                    >  1:1179183/1‑69 (MQ=255)
                                gATGACAATGCAATTATCACCACCGACGTTGGTCAg                                                                >  1:3364768/1‑36 (MQ=255)
                                gATGACAATGCAATTATCACCACCGACGTTGGTCAg                                                                >  1:1275492/1‑36 (MQ=255)
                                gATGACAATGCAATTATCACCACCGACGTTGGTCAGGATCAGATGTGGACCGCGCAAGCTTATCCGCTc                               >  1:1968724/1‑69 (MQ=255)
                                gATGACAATGCAATTATCACCACCGACGTTGGTCAGCATCAGATGTGGACCGCGCAAGCTTATCCGCt                                >  1:2834901/1‑68 (MQ=255)
                                gATGACAATGCAATTATCACCACCGACGTTGGTCAGCATCAGATGTGGACCGCGCAAGCTTATCCGCTc                               >  1:1562188/1‑69 (MQ=255)
                                gATGACAATGCAATTATCACCACCGACGTTGGTCAGCATCAGATGTGGACCGCGCAAGCTTATCCGCTc                               >  1:598057/1‑69 (MQ=255)
                                gATGACAATGCAATTATCACCACCGACGTTGGTCAGCATCAGATGTGGACCGCGCAAGCTTATCCGCTc                               >  1:652412/1‑69 (MQ=255)
                                gATGACAATGCAATTATCACCACCGACGTTGGTCAGCATCAGATGTGGACCGCGCAAGCTTATCCGCTc                               >  1:1291139/1‑69 (MQ=255)
                                gATGACAATGCAATTATCACCACCGACGTTGGTCAGCATCAGATGTGGACCGCGCAAGCTTATCCGCTc                               >  1:2658164/1‑69 (MQ=255)
                                      aaTGCAATTATCACCACCGACGTTGGTCAGCATCAGATGTGGACCGCGCaa                                           >  1:1053287/1‑51 (MQ=255)
                                      aaTGCAATTATCACCACCGACGTTGGTCAGCATCAGATGTGGACCGCGCAAg                                          >  1:989706/1‑52 (MQ=255)
                                      aaTGCAATTATCACCACCGACGTTGGTCAGCATCAGATGTGGACCGCGCAAg                                          <  1:2623376/52‑1 (MQ=255)
                                      aaTGCAATTATCACCACCGACGTTGGTCAGCATCAGATGTGGACCGCGCAAg                                          <  1:1391755/52‑1 (MQ=255)
                                      aaTGCAATTATCACCACCGACGTTGGTCAGCATCAGATGTGGACCGCGCAAg                                          >  1:1648264/1‑52 (MQ=255)
                                      aaTGCAATTATCACCACCGACGTTGGTCAGCATCAGATGTGGACCGCGCAAg                                          >  1:1781710/1‑52 (MQ=255)
                                         gCAATTATCACCACCGACGTTGGTCAGCATCAGATGTGGACCGCGCAAGCTTATCCGCTCAATAGcc                        >  1:1019774/1‑67 (MQ=255)
                                            aTTATCACCACCGACGTGGGTCAGCATCAGATGTGGACCGCGCAAGCCTATCCTCTCAATCGccc                       <  1:204628/65‑1 (MQ=255)
                                            aTTATCACCACCGACGTGGGTCAGCATCAGATGTGGACCGCGCAAGCCTATCCGCTCACTCGccc                       <  1:3463/65‑1 (MQ=255)
                                            aTTATCACCACCGACGTGGGTCAGCATCAGATGTGGACCGCGCAAGCCTATCCGCTCAATCGccc                       <  1:3319656/65‑1 (MQ=255)
                                            aTTATCACCACCGACGTGGGTCAGCATCAGATGTGGACCGCGCAAGCCTATCCGCTCAATCGccc                       <  1:1578970/65‑1 (MQ=255)
                                            aTTATCACCACCGACGTGGGTCAGCATCAGATGTGGACCGCGCAAGCCTATCCGCTCAATCGccc                       <  1:1577656/65‑1 (MQ=255)
                                            aTTATCACCACCGACGTGGGTCAGCATCAGATGTGGACCGCGCAAGCCTATCCGCTCAATCGccc                       <  1:373539/65‑1 (MQ=255)
                                            aTTATCACCACCGACGTGGGTCAGCATCAGATGTGGACCGCGCAAGCCTATCCGCTCAATCGccc                       <  1:479309/65‑1 (MQ=255)
                                            aTTATCACCACCGACGTGGGTCAGCATCAGATGTGGACCGCGCAAGCCTATCCGCTCAATCGccc                       <  1:3415227/65‑1 (MQ=255)
                                            aTTATCACCACCGACGTGGGTCAGCATCAGATGTGGACCGCGCAAGCCTATCCGCTCAATCGccc                       <  1:533057/65‑1 (MQ=255)
                                            aTTATCACCACCGACGTGGGTCAGCATCAGATGTGGACCGCGCAAGCCTATCCGCTCAATCGccc                       <  1:2693/65‑1 (MQ=255)
                                            aTTATCACCACCGACGTGGGTCAGCATCAGATGTGGACCGCGCAAGCCTATCCGCTCAATCGccc                       <  1:1917228/65‑1 (MQ=255)
                                            aTTATCACCACCGACGTGGGTCAGCATCAGATGTGGACCGCGCAAGCCTATCCGCTCAATCGccc                       <  1:1975392/65‑1 (MQ=255)
                                            aTTATCACCACCGACGTGGGTCAGCATCAGATGTGGACCGCGCAAGCCTATCCGCTCAATCGccc                       <  1:3087664/65‑1 (MQ=255)
                                            aTTATCACCACCGACGTGGGTCAGCATCAGATGTGGACCGCGCAAGCCTATCCGCTCAATCGccc                       <  1:2085378/65‑1 (MQ=255)
                                            aTTATCACCACCGACGTGGGTCAGCATCAGATGTGGACCGCGCAAGCCTATCCGCTCAATCGccc                       <  1:2665709/65‑1 (MQ=255)
                                            aTTATAACCACCGACGTGGGTCAGCATCAGATGTGGACCGCGCAAGACTATCCGCTCAATCGccc                       <  1:67214/65‑1 (MQ=255)
                                              tATCACCACCGACGTTGGTCAGCATCTGATGTGGACCGCGCAAGCTTATCCGCTCAATCGCCCACGcc                  <  1:2051233/68‑1 (MQ=255)
                                              tATCACCACCGACGTTGGTCAGCATCAGATGTGGACCGCGCAAGCTTATCCGCTCAATCGCCCACGcc                  <  1:1613160/68‑1 (MQ=255)
                                              tATCACCACCGACGTTGGTCAGCATCAGATGTGGACCGCGCAAGCTTATCCGCTCAATCGCCCACGcc                  <  1:1321695/68‑1 (MQ=255)
                                               aTCACCACTGACGTTGGTCAGCATCAGATGTGGACCGCGCAAGCTTATCCGCTCAATCGCCCACGCCAg                >  1:2314814/1‑69 (MQ=255)
                                               aTCACCACCGACGTTGGTCAGCATCAGATGTGGACCGCGCAAGCTTATCCGCTCAATCGCCCACGCCAg                >  1:217958/1‑69 (MQ=255)
                                                    ctccGACGTTGGTCAGCATCAGATGTGGACCGCGCAAGCTTATCCGCGCAATCGCCCACGCCAGTGGCt           <  1:383380/67‑1 (MQ=255)
                                                    caccGACGTTGGTCAGCATCAGATGTGGACCGCGCAAGCTTATCCGCTCAATCGCCCACGCCAGTGGCt           <  1:39184/69‑1 (MQ=255)
                                                    caccGACGTTGGTCAGCATCAGATGTGGACCGCGCAAGCTTATCCGCTCAATCGCCCACGCCAGTGGCt           <  1:1527736/69‑1 (MQ=255)
                                                             tGGTCAGCATCAGATGTGGACCGCGCAAGCTTATCCGCTCAATCGCCCACGCCAGTGGCTGACCTCCgg  >  1:258777/1‑69 (MQ=255)
                                                             tGGTCAGCATCAGATGTGGACCGCGCAAGCTTATCCGCTCAATCGCCCACGCCAGTGGCTGACCTCCgg  >  1:251198/1‑69 (MQ=255)
                                                             tGGTCAGCATCAGATGTGGACCGCGCAAGCTTATCCGCTCAATCGCCCACGCCAGTGGCTGACCTCCgg  >  1:3114867/1‑69 (MQ=255)
                                                             tGGTCAGCATCAGATGTGGACCGCGCAAGCTTATCCGCTCAATCGCCCACGCCAGTGGCTGACCTCCgg  >  1:3096622/1‑69 (MQ=255)
                                                             |                                                                    
ACGGCCTGATCAACGCCGTTGCCGCCTGTGTCGATGACAATGCAATTATCACCACCGACGTTGGTCAGCATCAGATGTGGACCGCGCAAGCTTATCCGCTCAATCGCCCACGCCAGTGGCTGACCTCCGG  >  minE/2498572‑2498701

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: