| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,499,173 | C→T | 60.8% | D7D (GAC→GAT) | ilvN → | acetolactate synthase I, small subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,499,173 | 0 | C | T | 60.8% | 46.6 / 69.9 | 102 | D7D (GAC→GAT) | ilvN | acetolactate synthase I, small subunit |
| Reads supporting (aligned to +/- strand): ref base C (22/18); new base T (27/35); total (49/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.12e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
Only 100 of 102 total aligned reads displayed.
GGTGCCGCCAGGTGCGGCGAATACTGAAATGGTGGGGGAATAAGCCATGCAAAACACAACTCATGACAACGTAATTCTGGAGCTCACCGTTCGCAACCATCCGGGCGTAATGACCCACGTTTGTGGCCTTTTTG > minE/2499107‑2499240 | ggTGCCGCCAGGTGCGGCGAATACTGAAATGGTGGGGGAATAAGCCATGCAAAACACAACTCATGACaa < 1:1748466/69‑1 (MQ=255)ggTGCCGCCAGGTGCGGCGAATACTGAAATGGTGGGGGAATAAGCCATGCAAAACACAACTCATGACaa < 1:424476/69‑1 (MQ=255)ggTGCCGCCAGGTGCGGCGAATACTGAAATGGTGGGGGAATAAGCCATGCAAAACACAACTCATGACaa < 1:2857466/69‑1 (MQ=255)ggTGCCGCCAGGTGCGGCGAATACTGAAATGGTGGGGGAATAAGCCATGCAAAACACAACTCATGACaa < 1:1508413/69‑1 (MQ=255) ccAGGTGCGGCGAATACTGAAATGGTGGGGGAATAAGCCATGCAAAACACAACTCATGATAACGTGAtt > 1:1415525/1‑69 (MQ=255) ccAGGTGCGGCGAATACTGAAATGGTGGGGGAATAAGCCATGCAAAACACAACTCATGATAACGTGAtt > 1:1691289/1‑69 (MQ=255) ccAGGTGCGGCGAATACTGAAATGGTGGGGGAATAAGCCATGCAAAACACAACTCATGATAACGTGAtt > 1:1827545/1‑69 (MQ=255) ccAGGTGCGGCGAATACTGAAATGGTGGGGGAATAAGCCATGCAAAACACAACTCATGATAACGTGAtt > 1:2258454/1‑69 (MQ=255) ccAGGTGCGGCGAATACTGAAATGGTGGGGGAATAAGCCATGCAAAACACAACTCATGATAACGTGAtt > 1:2398606/1‑69 (MQ=255) ccAGGTGCGGCGAATACTGAAATGGTGGGGGAATAAGCCATGCAAAACACAACTCATGATAACGTGAtt > 1:2584561/1‑69 (MQ=255) ccAGGTGCGGCGAATACTGAAATGGTGGGGGAATAAGCCATGCAAAACACAACTCATGATAACGTGAtt > 1:2592320/1‑69 (MQ=255) ccAGGTGCGGCGAATACTGAAATGGTGGGGGAATAAGCCATGCAAAACACAACTCATGATAACGTGAtt > 1:2690880/1‑69 (MQ=255) ccAGGTGCGGCGAATACTGAAATGGTGGGGGAATAAGCCATGCAAAACACAACTCATGATAACGTGAtt > 1:2740320/1‑69 (MQ=255) ccAGGTGCGGCGAATACTGAAATGGTGGGGGAATAAGCCATGCAAAACACAACTCATGATAACGTGAtt > 1:1412466/1‑69 (MQ=255) ccAGGTGCGGCGAATACTGAAATGGTGGGGGAATAAGCCATGCAAAACACAACTCATGATAACGTGAtt > 1:2793210/1‑69 (MQ=255) ccAGGTGCGGCGAATACTGAAATGGTGGGGGAATAAGCCATGCAAAACACAACTCATGATAACGTGAtt > 1:2813635/1‑69 (MQ=255) ccAGGTGCGGCGAATACTGAAATGGTGGGGGAATAAGCCATGCAAAACACAACTCATGATAACGTGAtt > 1:319136/1‑69 (MQ=255) ccAGGTGCGGCGAATACTGAAATGGTGGGGGAATAAGCCATGCAAAACACAACTCATGATAACGTGAtt > 1:3323559/1‑69 (MQ=255) ccAGGTGCGGCGAATACTGAAATGGTGGGGGAATAAGCCATGCAAAACACAACTCATGATAACGTGAtt > 1:575768/1‑69 (MQ=255) ccAGGTGCGGCGAATACTGAAATGGTGGGGGAATAAGCCATGCAAAACACAACTCATGATAACGTGAtt > 1:62829/1‑69 (MQ=255) ccAGGTGCGGCGAATACTGAAATGGTGGGGGAATAAGCCATGCAAAACACAACTCATGATAACGTGAtt > 1:1089139/1‑69 (MQ=255) ccAGGTGCGGCGAATACTGAAATGGTGGGGGAATAAGCCATGCAAAACACAACTCATGATAACGTGAtt > 1:644406/1‑69 (MQ=255) ccAGGTGCGGCGAATACTGAAATGGTGGGGGAATAAGCCATGCAAAACACAACTCATGATAACGTGAtt > 1:800747/1‑69 (MQ=255) ccAGGTGCGGCGAATACTGAAATGGTGGGGGAATAAGCCATGCAAAACACAACTCATGATAACGTGAtt > 1:801080/1‑69 (MQ=255) aTACTGAAATGGTGGGGGAATAAGCCATGCAAAACACAACTCATGACaa < 1:2754680/49‑1 (MQ=255) aCTGAAATGGTGGGGGAATAAGCCATGCAAAACACAACTCATGACAACGTAATTc < 1:958278/55‑1 (MQ=255) aCTGAAATGGTGGGGGAATAAGCCATGCAAAACACAACTCATGACAACGTAATTc < 1:23972/55‑1 (MQ=255) aCTGAAATGGTGGGGGAATAAGCCATGCAAAACACAACTCATGACAACGTAATTc < 1:1748123/55‑1 (MQ=255) aCTGAAATGGTGGGGGAATAAGCCATGCAAAACACAACTCATGACAACGTAATTc < 1:168135/55‑1 (MQ=255) aCTGAAATGGTGGGGGAATAAGCCATGCAAAACACAACTCATGACAACGTAATTc < 1:1007858/55‑1 (MQ=255) cTGAAATGGTGGGGGAATAAGCCATGCAAAACACAACTCATGACAACGTAAt > 1:1839674/1‑52 (MQ=255) cTGAAATGGTGGGGGAATAAGCCATGCAAAACACAACTCATGACAACGTAATTCTGGAGCTCACCGtt > 1:2744850/1‑68 (MQ=255) cTGAAATGGTGGGGGAATAAGCCATGCAAAACACAACTCATGACAACGTAATTCTGGAGCTCACCGtt > 1:2722072/1‑68 (MQ=255) cTGAAATGGTGGGGGAATAAGCCATGCAAAACACAACTCATGACAACGTAATTCTGGAGCTCACCGtt > 1:1539153/1‑68 (MQ=255) cTGAAATGGTGGGGGAATAAGCCATGCAAAACACAACTCATGACAACGTAATTCTGGAGCTCACCGtt > 1:3138310/1‑68 (MQ=255) cTGAAATGGTGGGGGAATAAGCCATGCAAAACACAACTCATGACAACGTAATTCTGGAGCTCACCGtt > 1:3192199/1‑68 (MQ=255) cTGAAATGGTGGGGGAATAAGCCATGCAAAACACAACTCATGACAACGTAATTCTGGAGCTCACCGtt > 1:1772236/1‑68 (MQ=255) tGAAATGGTGGTGTAATAAGCCATGCAAAACACAACTCATGATAACGTGATTCTGGAACTCACCGt < 1:886429/66‑1 (MQ=255) tGAAATGGTGGGGGATTAAGCCATGCAAAACACAACTCATGATAACGTGATTCTGGAACTCACCGt < 1:2789458/66‑1 (MQ=255) tGAAATGGTGGGGGAATAAGCCATGCAAAACACAACTCATGATAACGTGATTCTGGAACTCACCGt < 1:452429/66‑1 (MQ=255) tGAAATGGTGGGGGAATAAGCCATGCAAAACACAACTCATGATAACGTGATTCTGGAACTCACCGt < 1:1530582/66‑1 (MQ=255) tGAAATGGTGGGGGAATAAGCCATGCAAAACACAACTCATGATAACGTGATTCTGGAACTCACCGt < 1:2621958/66‑1 (MQ=255) tGAAATGGTGGGGGAATAAGCCATGCAAAACACAACTCATGATAACGTGATTCTGGAACTCACCGt < 1:278605/66‑1 (MQ=255) tGAAATGGTGGGGGAATAAGCCATGCAAAACACAACTCATGATAACGTGATTCTGGAACTCACCGt < 1:2479652/66‑1 (MQ=255) tGAAATGGTGGGGGAATAAGCCATGCAAAACACAACTCATGATAACGTGATTCTGGAACTCACCGt < 1:2729349/66‑1 (MQ=255) tGAAATGGTGGGGGAATAAGCCATGCAAAACACAACTCATGATAACGTGATTCTGGAACTCACCGt < 1:1547282/66‑1 (MQ=255) tGAAATGGTGGGGGAATAAGCCATGCAAAACACAACTCATGATAACGTGATTCTGGAACTCACCGt < 1:95261/66‑1 (MQ=255) tGAAATGGTGGGGGAATAAGCCATGCAAAACACAACTCATGATAACGTGATTCTGGAACTCACCGt < 1:1452635/66‑1 (MQ=255) tGAAATGGTGGGGGAATAAGCCATGCAAAACACAACTCATGATAACGTGATTCTGGAACTCACCGt < 1:3416282/66‑1 (MQ=255) tGAAATGGTGGGGGAATAAGCCATGCAAAACACAACTCATGATAACGTGATTCTGGAACTCACCGt < 1:371587/66‑1 (MQ=255) tGAAATGGTGGGGGAATAAGCCATGCAAAACACAACTCATGATAACGTGATTCTGGAACTCACCGt < 1:1225777/66‑1 (MQ=255) tGAAATGGTGGGGGAATAAGCCATGCAAAACACAACTCATGATAACGTGATTCTGCAACTCACCGt < 1:3311534/66‑1 (MQ=255) gAAATGGTGGGGGAATAAGCCATGCAAAACACAACTCATGATAACGTGATTCTGGAACTCACCGt < 1:2974152/65‑1 (MQ=255) gAAATGGTGGGGGAATAAGCCATGCAAAACACAACTCATGATAACGTGATTCTGGAACTCACCGt < 1:2847448/65‑1 (MQ=255) gAAATGGTGGGGGAATAAGCCATGCAAAACACAACTCATGATAACGTGATTCTGGAACTCACCGt < 1:379791/65‑1 (MQ=255) tggGGGAATAAGCCATGCAAAACACAACTCATGATAACGTGATTCTGGAACt > 1:2958952/1‑52 (MQ=255) tggGGGAATAAGCCATGCAAAACACAACTCATGATAACGTGATTCTGGAACTCACCGTTCGCAACCATc > 1:1715448/1‑69 (MQ=255) tggGGGAATAAGCCATGCAAAACACAACTCATGATAACGTGATTCTGGAACTCACCGTTCGCAACCATc > 1:1155852/1‑69 (MQ=255) tggGGGAATAAGCCATGCAAAACACAACTCATGATAACGTGATTCTGGAACTCACCGTTCGCAACCATc > 1:1240648/1‑69 (MQ=255) tggGGGAATAAGCCATGCAAAACACAACTCATGATAACGTGATTCTGGAACTCACCGTTCGCAACCATc > 1:1002057/1‑69 (MQ=255) tggGGGAATAAGCCATGCAAAACACAACTCATGATAACGTGATTCTGGAACTCACCGTTCGCAACCATc > 1:1544808/1‑69 (MQ=255) tggGGGAATAAGCCATGCAAAACACAACTCATGATAACGTGATTCTGGAACTCACCGTTCGCAACCATc > 1:1465920/1‑69 (MQ=255) aaTAAGCCATGCAAAACACAACTCATGACAACGTAATTCTGGAGCTCACCGTTCGCAACCATCCGGGc > 1:419493/1‑68 (MQ=255) aaGCCATGCAAAACACAACTCATGATAACGTGATTCTGGAACTCACCGt < 1:908172/49‑1 (MQ=38) ccATGCAAAACACAAATCATGATAACGTGATTCTTGAACTCACCGTTCGCAACCATCCGGGTGTAATGa < 1:1386885/69‑1 (MQ=255) ccATGCAAAACACAAATCATGATAACGTGATTCTGGAACTCACCGTTCGCAACCATCCGGGTGTAATGa < 1:2165481/69‑1 (MQ=255) ccATGCAAAACACAAATCATGATAACGTGATTCTGGAACTCACCGTTCGCAACCATCCGGGTGTAATGa < 1:1578105/69‑1 (MQ=255) ccATGCAAAACACAAATCATGATAACGTGATTCTGGAACTCACCGTTCGCAACCATCCGGGTGTAATGa < 1:1814379/69‑1 (MQ=255) ccATGCAAAACACAAATCATGATAACGTGATTCTGGAACTCACCGTTCGCAACCATCCGGGTGTAATGa < 1:157715/69‑1 (MQ=255) ccATGCAAAACACAAATCATGATAACGTGATTCTGGAACTCACCGTTCGCAACCATCCGGGTGTAATGa < 1:1972595/69‑1 (MQ=255) ccATGCAAAACACAAATCATGATAACGTGATTCTGGAACTCACCGTTCGCAACCATCCGGGTGTAATGa < 1:1048444/69‑1 (MQ=255) ccATGCAAAACACAAATCATGATAACGTGATTCTGGAACTCACCGTTCGCAACCATCCGGGTGTAATGa < 1:2074895/69‑1 (MQ=255) ccATGCAAAACACAAATCATGATAACGTGATTCTGGAACTCACCGTTCGCAACCATCCGGGTGTAATGa < 1:2112520/69‑1 (MQ=255) ccATGCAAAACACAAATCATGATAACGTGATTCTGGAACTCACCGTTCGCAACCATCCGGGTGTAATGa < 1:2184781/69‑1 (MQ=255) ccATGCAAAACACAAATCATGATAACGTGATTCTGGAACTCACCGTTCGCAACCATCCGGGTGTAATGa < 1:2188644/69‑1 (MQ=255) ccATGCAAAACACAAATCATGATAACGTGATTCTGGAACTCACCGTTCGCAACCATCCGGGTGTAATGa < 1:2586378/69‑1 (MQ=255) ccATGCAAAACACAAATCATGATAACGTGATTCTGGAACTCACCGTTCGCAACCATCCGGGTGTAATGa < 1:3095096/69‑1 (MQ=255) ccATGCAAAACACAAATCATGATAACGTGATTCTGGAACTCACCGTTCGCAACCATCCGGGTGTAATGa < 1:2444778/69‑1 (MQ=255) ccATGCAAAACACAAATCATGATAACGTGATTCTGGAACTCACCGTTCGCAACCATCCGGGTGTAATGa < 1:129523/69‑1 (MQ=255) cAAAACACAACTCATGACAACGTAATTCTGGAGCTCACCGTTCGCAACCATCCGGGCGTAATGACCCAc > 1:1572744/1‑69 (MQ=255) cAAAACACAACTCATGACAACGTAATTCTGGAGCTCACCGTTCGCAACCATCCGGGCGTAATGACCCAc > 1:3046642/1‑69 (MQ=255) cAAAACACAACTCATGACAACGTAATTCTGGAGCTCACCGTTCGCAACCATCCGGGCGTAATGACCCAc > 1:2905902/1‑69 (MQ=255) cAAAACACAACTCATGACAACGTAATTCTGGAGCTCACCGTTCGCAACCATCCGGGCGTAATGACCCAc > 1:700130/1‑69 (MQ=255) cacaACTCATGACAACGTAATTCTGGAGCTCACCGTTCGCAACCATCCGGGCGTAATGACCCACGTTtg > 1:3091701/1‑69 (MQ=255) cacaACTCATGACAACGTAATTCTGGAGCTCACCGTTCGCAACCATCCGGGCGTAATGACCCACGTTtg > 1:154841/1‑69 (MQ=255) cacaACTCATGACAACGTAATTCTGGAGCTCACCGTTCGCAACCATCCGGGCGTAATGACCCACGTTtg > 1:1333135/1‑69 (MQ=255) cacaACTCATGACAACGTAATTCTGGAGCTCACCGTTCGCAACCATCCGGGCGTAATGACCCACGTTtg > 1:725543/1‑69 (MQ=255) cacaACTCATGACAACGTAATTCTGGAGCTCACCGTTCGCAACCATCCGGGCGTAATGACCCACGTTtg > 1:472443/1‑69 (MQ=255) cacaACTCATGACAACGTAATTCTGGAGCTCACCGTTCGCAACCATCCGGGCGTAATGACCCACGTTtg > 1:2020776/1‑69 (MQ=255) cATGACAACGTAATTCTGGAGCTCACCGTTCGCAACCATCCGGGCGTAATGACCCACGTTTGTGGCCtt < 1:2608797/69‑1 (MQ=255) cATGACAACGTAATTCTGGAGCTCACCGTTCGCAACCATCCGGGCGTAATGACCCACGTTTGTGGCCtt < 1:2200045/69‑1 (MQ=255) aCAACGTAATTCTGGAGCTCACCGTTCGCAACCATCCGGGCGTAATGa < 1:1080347/48‑1 (MQ=255) aCAACGTAATTCTGGAGCTCACCGTTCGCAACCATCCGGGCGTAATGa < 1:470228/48‑1 (MQ=255) aCAACGTAATTCTGGAGCTCACCGTTCGCAACCATCCGGGCGTAATGa < 1:2031616/48‑1 (MQ=255) aCAACGTAATTCTGGAGCTCACCGTTCGCAACCATCCGGGCGTAATGa < 1:290662/48‑1 (MQ=255) aCAACGTAATTCTGGAGCTCACCGTTCGCAACCATCCGGGCGTAATGa < 1:1281247/48‑1 (MQ=255) aCAACGTAATTCTGGAGCTCACCGTTCGCAACCATCCGGGCGTAATGACCCACGTTTGTGGCCTTTTTg > 1:664743/1‑69 (MQ=255) aCAACGTAATTCTGGAGCTCACCGTTCGCAACCATCCGGGCGTAATGACCCACGTTTGTGGCCTTTTTg > 1:1136960/1‑69 (MQ=255) aCAACGTAATTCTGGAGCTCACCGTTCGCAACCATCCGGGCGTAATGACCCACGTTTGTGGCCTTTTTg > 1:2838723/1‑69 (MQ=255) aCAACGTAATTCTGGAGCTCACCGTTCGCAACCATCCGGGCGTAATGACCCACGTTTGTGGCCTTTTTg > 1:2091807/1‑69 (MQ=255) | GGTGCCGCCAGGTGCGGCGAATACTGAAATGGTGGGGGAATAAGCCATGCAAAACACAACTCATGACAACGTAATTCTGGAGCTCACCGTTCGCAACCATCCGGGCGTAATGACCCACGTTTGTGGCCTTTTTG > minE/2499107‑2499240 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |