| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,499,305 | T→C | 60.4% | H51H (CAT→CAC) | ilvN → | acetolactate synthase I, small subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,499,305 | 0 | T | C | 60.4% | 31.8 / 43.9 | 68 | H51H (CAT→CAC) | ilvN | acetolactate synthase I, small subunit |
| Reads supporting (aligned to +/- strand): ref base T (11/16); new base C (17/24); total (28/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 | |||||||||||
CCGCGCTTTTAACGTTGAAGGCATTCTTTGTCTGCCGATTCAGGACAGCGACAAAAGCCATATCTGGCTACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAA > minE/2499245‑2499371 | ccgcGCTTTTAACGTTGAAGGCATTCTTTGTCTGCCGATTCAGGACAGCGACAAAAGCCATATCTGGCt < 1:2673054/69‑1 (MQ=255)ccgcGCTTTTAACGTTGAAGGCATTCTTTGTCTGCCGATTCAGGACAGCGACAAAAGCCATATCTGGCt < 1:2365219/69‑1 (MQ=255)ccgcGCTTTTAACGTTGAAGGCATTCTTTGTCTGCCGATTCAGGACAGCGACAAAAGCCATATCTGGCt < 1:1420857/69‑1 (MQ=255)ccgcGCTTTTAACGTTGAAGGCATTCTTTGTCTGCCGATTCAGGACAGCGACAAAAGCCATATCTGGCt < 1:1675384/69‑1 (MQ=255) cTTTTAACGTTGAAGGCATTCTCTGCCTGCCGATTCAGGACAGCGACAAAAGCCACATCTGGCTACTgg < 1:1020739/69‑1 (MQ=255) cTTTTAACGTTGAAGGCATTCTCTGCCTGCCGATTCAGGACAGCGACAAAAGCCACATCTGGCTACTgg < 1:2513609/69‑1 (MQ=255) cTTTTAACGTTGAAGGCATTCTCTGCCTGCCGATTCAGGACAGCGACAAAAGCCACATCTGGCTACTgg < 1:2888197/69‑1 (MQ=255) cTTTTAACGTTGAAGGCATTCTCTGCCTGCCGATTCAGGACAGCGACAAAAGCCACATCTGGCTACTgg < 1:2935262/69‑1 (MQ=255) cTTTTAACGTTGAAGGCATTCTCTGCCTGCCGATTCAGGACAGCGACAAAAGCCACATCTGGCTACTgg < 1:2285439/69‑1 (MQ=255) cTTTTAACGTTGAAGGCATTCTCTGCCTGCCGATTCAGGACAGCGACAAAAGCCACATCTGGCTACTgg < 1:2258635/69‑1 (MQ=255) cTTTTAACGTTGAAGGCATTCTCTGCCTGCCGATTCAGGACAGCGACAAAAGCCACATCTGGCTACTgg < 1:2249797/69‑1 (MQ=255) cTTTTAACGTTGAAGGCATTCTCTGCCTGCCGATTCAGGACAGCGACAAAAGCCACATCTGGCTACTgg < 1:3069841/69‑1 (MQ=255) cTTTTAACGTTGAAGGCATTCTCTGCCTGCCGATTCAGGACAGCGACAAAAGCCACATCTGGCTACTgg < 1:3386407/69‑1 (MQ=255) cTTTTAACGTTGAAGGCATTCTCTGCCTGCCGATTCAGGACAGCGACAAAAGCCACATCTGGCTACTgg < 1:160179/69‑1 (MQ=255) cTTTTAACGTTGAAGGCATTCTCTGCCTGCCGATTCAGGACAGCGACAAAAGCCACATCTGGCTACTgg < 1:827241/69‑1 (MQ=255) cTTTTAACGTTGAAGGCATTCTCTGCCTGCCGATTCAGGACAGCGACAAAAGCCACATCTGGCTACTgg < 1:722486/69‑1 (MQ=255) cTTTTAACGTTGAAGGCATTCTCTGCCTGCCGATTCAGGACAGCGACAAAAGCCACATCTGGCTACTgg < 1:1280046/69‑1 (MQ=255) cTTTTAACGTTGAAGGCATTCTCTGCCTGCCGATTCAGGACAGCGACAAAAGCCACATCTGGCTACTgg < 1:1431411/69‑1 (MQ=255) ttttAACGTTGAAGGCATTCTCTGCCTGCCGATTCAGGACAGCGACAAAAGCCACATCTGGCTACTgg < 1:383060/68‑1 (MQ=255) ttttAACGTTGAAGGCATTCTCTGCCTGCCGATTCAGGACAGCGACAAAAGCCACATCTGGCTACTgg < 1:3025838/68‑1 (MQ=255) ttttAACGTTGAAGGCATTCTCTGCCTGCCGATTCAGGACAGCGACAAAAGCCACATCTGGCTACTgg < 1:54471/68‑1 (MQ=255) ttttAACGTTGAAGGCATTCTCTGCCTGCCGATTCAGGACAGCGACAAAAGCCACATCTGGCTACTgg < 1:1466063/68‑1 (MQ=255) ttttAACGTTGAAGGCATTCTCTGCCTGCCGATTCAGGACAGCGACAAAAGCCACATCTGGCTACTgg < 1:1794634/68‑1 (MQ=255) ttttAACGTTGAAGGCATTCTCTGCCTGCCGATTCAGGACAGCGACAAAAGCCACATCTGGCTACTgg < 1:1696039/68‑1 (MQ=255) ttttAACGTTGAAGGCATTCTCTGCCTGCCGATTCAGGACAGCGACAAAAGCCACATCTGGCTACTgg < 1:3351464/68‑1 (MQ=255) tAACGTTTAAGGCATTCTTTGTCTGCCGATTCAGGACAGCGACAAAAGCCATATCTGGCt < 1:2864696/60‑1 (MQ=255) tAACGTTGAAGGCATTCTCTGCCTGCCGATTCAGGACAGCGACAAAAGCCACATCTGGCTACTGGTCa > 1:3208669/1‑68 (MQ=255) tAACGTTGAAGGCATTCTCTGCCTGCCGATTCAGGACAGCGACAAAAGCCACATCTGGCTACTGGTCa > 1:3302426/1‑68 (MQ=255) tAACGTTGAAGGCATTCTCTGCCTGCCGATTCAGGACAGCGACAAAAGCCACATCTGGCTACTGGTCa > 1:3321198/1‑68 (MQ=255) tAACGTTGAAGGCATTCTCTGCCTGCCGATTCAGGACAGCGACAAAAGCCACATCTGGCTACTGGTCa > 1:3018972/1‑68 (MQ=255) tAACGTTGAAGGCATTCTCTGCCTGCCGATTCAGGACAGCGACAAAAGCCACATCTGGCTACTGGTCa > 1:637253/1‑68 (MQ=255) tAACGTTGAAGGCATTCTCTGCCTGCCGATTCAGGACAGCGACAAAAGCCACATCTGGCTACTGGTCa > 1:918133/1‑68 (MQ=255) tAACGTTGAAGGCATTCTCTGCCTGCCGATTCAGGACAGCGACAAAAGCCACATCTGGCTACTGGTCa > 1:283826/1‑68 (MQ=255) tAACGTTGAAGGCATTCTCTGCCTGCCGATTCAGGACAGCGACAAAAGCCACATCTGGCTACTGGTCa > 1:2808826/1‑68 (MQ=255) tAACGTTGAAGGCATTCTCTGCCTGCCGATTCAGGACAGCGACAAAAGCCACATCTGGCTACTGGTCa > 1:945077/1‑68 (MQ=255) tAACGTTGAAGGCATTCTCTGCCTGCCGATTCAGGACAGCGACAAAAGCCACATCTGGCTACTGGTCa > 1:2611434/1‑68 (MQ=255) tAACGTTGAAGGCATTCTCTGCCTGCCGATTCAGGACAGCGACAAAAGCCACATCTGGCTACTGGTCa > 1:2605327/1‑68 (MQ=255) tAACGTTGAAGGCATTCTCTGCCTGCCGATTCAGGACAGCGACAAAAGCCACATCTGGCTACTGGTCa > 1:2736170/1‑68 (MQ=255) tAACGTTGAAGGCATTCTCTGCCTGCCGATTCAGGACAGCGACAAAAGCCACATCTGGCTACTGGTCa > 1:1189577/1‑68 (MQ=255) tAACGTTGAAGGCATTCTCTGCCTGCCGATTCAGGACAGCGACAAAAGCCACATCTGGCTACTGGTCa > 1:22770/1‑68 (MQ=255) tAACGTTGAAGGCATTCTCTGCCTGCCGATTCAGGACAGCGACAAAAGCCACATCTGGCTACTGGTCa > 1:2235071/1‑68 (MQ=255) tAACGTTGAAGGCATTCTCTGCCTGCCGATTCAGGACAGCGACAAAAGCCACATCTGGCTACTGGTCa > 1:1083857/1‑68 (MQ=255) tAACGTTGAAGGCATTCTCTGCCTGCCGATTCAGGACAGCGACAAAAGCCACATCTGGCTACTGGTCa > 1:1883640/1‑68 (MQ=255) gAAGGCATTCTCTGCCTGCCGATTCAGGACAGCGACAAAAGCCACATCTGGCTACTgg < 1:2979480/58‑1 (MQ=255) tctcTGCCTGACGATTCAGGACAGCGACAAAAGCCACATCTGGCTACCgg < 1:1462347/50‑1 (MQ=255) tCTGCCGATTCAGGACAGCGACAAAAGCCATATCTGGCTACTGGTCAATGACGACCAGCGTCTgg > 1:3280902/1‑65 (MQ=255) gCCGATTCAGGACAGCGACAAAAGCCACATCTGGCTACTgg < 1:1669747/41‑1 (MQ=255) gATTCTGGACAGCGACAAAAGCCATATCTGGCTACTGGTCAATGACGACCAGCGTCTGGAGCAgatga < 1:1553303/68‑1 (MQ=255) gATTCAGGACAGCGACAAAAGCCATATCTGGCTACTGGTCAATGACGACCAGCGTCTGGAGCAgatga < 1:175962/68‑1 (MQ=255) gATTCAGGACAGCGACAAAAGCCATATCTGGCTACTGGTCAATGACGACCAGCGTCTGGAGCAgatga < 1:2785319/68‑1 (MQ=255) gATTCAGGACAGCGACAAAAGCCATATCTGGCTACTGGTCAATGACGACCAGCGTCTGGAGCAgatga < 1:1095656/68‑1 (MQ=255) gATTCAGGACAGCGACAAAAGCCATATCTGGCTACTGGTCAATGACGACCAGCGTCTGGAGCAgatga < 1:1271585/68‑1 (MQ=255) gATTCAGGACAGCGACAAAAGCCATATCTGGCTACTGGTCAATGACGACCAGCGTCTGGAGCAgatga < 1:855664/68‑1 (MQ=255) gCGACAAAAGCCATATCTGGCTACTGGTCAATGACGACCAGc > 1:232922/1‑42 (MQ=255) gCGACAAAAGCCATATCTGGCTACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATc > 1:1692380/1‑68 (MQ=255) gCGACAAAAGCCATATCTGGCTACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATc > 1:3246365/1‑68 (MQ=255) gCGACAAAAGCCATATCTGGCTACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATc > 1:2222860/1‑68 (MQ=255) gCGACAAAAGCCATATCTGGCTACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATc > 1:3398034/1‑68 (MQ=255) gCGACAAAAGCCATATCTGGCTACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATc > 1:2382471/1‑68 (MQ=255) gCGACAAAAGCCATATCTGGCTACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATc > 1:2588256/1‑68 (MQ=255) cAAAAGCCATATCTGGCTACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATaa > 1:1063195/1‑69 (MQ=255) cAAAAGCCATATCTGGCTACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATaa > 1:566993/1‑69 (MQ=255) cAAAAGCCATATCTGGCTACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATaa > 1:452707/1‑69 (MQ=255) cATATCTGGCTACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGaa < 1:744977/69‑1 (MQ=255) cATATCTGGCTACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGaa < 1:3368991/69‑1 (MQ=255) cATATCTGGCTACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGaa < 1:3321115/69‑1 (MQ=255) cATATCTGGCTACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGaa < 1:31617/69‑1 (MQ=255) cATATCTGGCTACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGaa < 1:2504225/69‑1 (MQ=255) | CCGCGCTTTTAACGTTGAAGGCATTCTTTGTCTGCCGATTCAGGACAGCGACAAAAGCCATATCTGGCTACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAA > minE/2499245‑2499371 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |