| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,499,356 | A→G | 32.8% | Q68Q (CAA→CAG) | ilvN → | acetolactate synthase I, small subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,499,356 | 0 | A | G | 32.8% | 191.5 / 128.0 | 228 | Q68Q (CAA→CAG) | ilvN | acetolactate synthase I, small subunit |
| Reads supporting (aligned to +/- strand): ref base A (92/61); new base G (58/17); total (150/78) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.15e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.64e-01 | |||||||||||
Only 100 of 228 total aligned reads displayed.
GCGACAAAAGCCATATCTGGCTACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGTTTAACAAG > minE/2499292‑2499422 | gCGACAAAAGCCATATCTGGCTACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATc > 1:3398034/1‑68 (MQ=255)gCGACAAAAGCCATATCTGGCTACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATc > 1:1692380/1‑68 (MQ=255) cAAAAGCCATATCTGGCTACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATaa > 1:566993/1‑69 (MQ=255) cATATCTGGCTACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGaa < 1:3321115/69‑1 (MQ=255) cATATCTGGCTACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGaa < 1:744977/69‑1 (MQ=255) tCTGGCTACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATg > 1:3171597/1‑69 (MQ=255) tCTGGCTACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATg > 1:3003726/1‑69 (MQ=255) tCTGGCTACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATg > 1:2743225/1‑69 (MQ=255) tCTGGCTACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATg > 1:2691022/1‑69 (MQ=255) tCTGGCTACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATg > 1:1561729/1‑69 (MQ=255) tCTGGCTACTGGTCAATGACGACAAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATg > 1:2386228/1‑69 (MQ=255) cTACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGat < 1:3294408/60‑2 (MQ=255) tACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAGATAGACAAGCTGGAAGATGTGGTGa < 1:1006113/69‑1 (MQ=255) tACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAGATAGACAAGCTGGAAGATGTGGTGa < 1:672159/69‑1 (MQ=255) tACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAGATAGACAAGCTGGAAGATGTGGTGa < 1:2196881/69‑1 (MQ=255) tACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAGATAGACAAGCTGGAAGATGTGGTGa < 1:1359355/69‑1 (MQ=255) tACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGt < 1:492931/67‑1 (MQ=255) tACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGt < 1:1718602/67‑1 (MQ=255) aCTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTTGAAGATGTCGTGaa < 1:1741600/69‑1 (MQ=255) tCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGc > 1:1384922/1‑69 (MQ=255) tCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGc > 1:1871687/1‑69 (MQ=255) cAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCa < 1:134609/69‑1 (MQ=255) tGACGACCAGCGTCTGGAGCAGATGATAAGCCAGATAGACAAGCTGGAAGATGTGGTGa < 1:2469463/59‑1 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGt > 1:2792189/1‑68 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGt > 1:2794792/1‑68 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGt > 1:1174072/1‑68 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGt > 1:2617786/1‑68 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGt > 1:1233685/1‑68 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGt > 1:1526994/1‑68 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGt > 1:334877/1‑68 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTa > 1:2913452/1‑69 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTa > 1:2972701/1‑69 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTa > 1:1147297/1‑69 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTa > 1:850075/1‑69 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTa > 1:93534/1‑69 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTa > 1:825426/1‑69 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTa > 1:3327822/1‑69 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTa > 1:2021266/1‑69 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTa > 1:236581/1‑69 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTa > 1:1214075/1‑69 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTa > 1:1881291/1‑69 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTa > 1:1649096/1‑69 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTa > 1:1850873/1‑69 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTa > 1:660391/1‑69 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTa > 1:1739281/1‑69 (MQ=255) gCGTCTGGAGCAGATGATAAGCCAGATAGACAAGCTGGAAGATGTGGTGAAAGTGCAGCGTAATCAg > 1:1664099/1‑67 (MQ=255) gCGTCTGGAGCAGATGATAAGCCAGATAGACAAGCTGGAAGATGTGGTGAAAGTGCAGCGTAATCAGt > 1:2884434/1‑68 (MQ=255) gCGTCTGGAGCAGATGATAAGCCAGATAGACAAGCTGGAAGATGTGGTGAAAGTGCAGCGTAATCAGt > 1:150394/1‑68 (MQ=255) gCGTCTGGAGCAGATGATAAGCCAGATAGACAAGCTGGAAGATGTGGTGAAAGTGCAGCGTAATCAGt > 1:2280330/1‑68 (MQ=255) gCGTCTGGAGCAGATGATAAGCCAGATAGACAAGCTGGAAGATGTGGTGAAAGTGCAGCGTAATCAGt > 1:2072466/1‑68 (MQ=255) gCGTCTGGAGCAGATGATAAGCCAGATAGACAAGCTGGAAGATGTGGTGAAAGTGCAGCGTAATCAGt > 1:1970618/1‑68 (MQ=255) gCGTCTGGAGCAGATGATAAGCCAGATAGACAAGCTGGAAGATGTGGTGAAAGTGCAGCGTAATCAGt > 1:1430627/1‑68 (MQ=255) tCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGtccga > 1:632967/1‑69 (MQ=255) gCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCcgac < 1:1417309/69‑1 (MQ=255) gCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCcgac < 1:1767899/69‑1 (MQ=255) aGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCcgacga < 1:2007952/69‑1 (MQ=255) gatgatAAGCCAGATAGACAAGCTGGAAGATGTGGTGa > 1:1499919/1‑38 (MQ=21) gatgatAAGCCAGATAGACAAGCTGGAAGATGTGGTGa > 1:425562/1‑38 (MQ=21) gatgatAAGCCAGATAGACAAGCTGGAAGATGTGGTGa > 1:1057773/1‑38 (MQ=21) gatgatAAGCCAGATAGACAAGCTGGAAGATGTGGTGa > 1:2440383/1‑38 (MQ=21) gatgatAAGCCAGATAGACAAGCTGGAAGATGTGGTGa > 1:1319323/1‑38 (MQ=21) gatgatAAGCCAGATAGACAAGCTGGAAGATGTGGTGa > 1:1904728/1‑38 (MQ=21) gatgatAAGCCAGATAGACAAGCTGGAAGATGTGGTGa > 1:3336750/1‑38 (MQ=21) tgatAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATg > 1:2181262/1‑68 (MQ=255) gatAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGt > 1:899623/1‑68 (MQ=255) gatAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGt > 1:1210174/1‑68 (MQ=255) atAAGCCAAATCGCTAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGttt < 1:3180195/69‑1 (MQ=255) atAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGttt < 1:1165509/69‑1 (MQ=255) atAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGttt < 1:1293086/69‑1 (MQ=255) atAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGttt < 1:2054402/69‑1 (MQ=255) atAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGttt < 1:1068410/69‑1 (MQ=255) atAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGttt < 1:2357912/69‑1 (MQ=255) tAAGCCAGATAGACAAGCTGGAAGATGTGGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGttt > 1:2265892/1‑68 (MQ=255) tAAGCCAGATAGACAAGCTGGAAGATGTGGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGttt > 1:3383813/1‑68 (MQ=255) tAAGCCAGATAGACAAGCTGGAAGATGTGGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGttt > 1:2956874/1‑68 (MQ=255) tAAGCCAGATAGACAAGCTGGAAGATGTGGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGttt > 1:3270343/1‑68 (MQ=255) tAAGCCAGATAGACAAGCTGGAAGATGTGGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGttt > 1:2208806/1‑68 (MQ=255) tAAGCCAGATAGACAAGCTGGAAGATGTGGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGttt > 1:1260119/1‑68 (MQ=255) tAAGCCAGATAGACAAGCTGGAAGATGTGGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGttt > 1:499468/1‑68 (MQ=255) tAAGCCAGATAGACAAGCTGGAAGATGTGGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGttt > 1:180932/1‑68 (MQ=255) tAAGCCAGATAGACAAGCTGGAAGATGTGGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGtt > 1:3026608/1‑67 (MQ=255) tAAGCCAGATAGACAAGCTGGAAGATGTGGTGAAAGTGCAGCGTAATCAGTCAGATCCGACGATGttt > 1:341722/1‑68 (MQ=255) tAAGCCAGATAGACAAGCTGGAAGATCTGGTGAAAGTGc > 1:2714704/1‑39 (MQ=16) tAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGttt > 1:724064/1‑68 (MQ=255) tAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGttt > 1:103787/1‑68 (MQ=255) tAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGttt > 1:2626278/1‑68 (MQ=255) tAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGttt > 1:1041903/1‑68 (MQ=255) aaGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGTTTa < 1:3062961/68‑1 (MQ=255) aaGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGTTTa < 1:2323148/68‑1 (MQ=255) aaGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGTTTa < 1:2235308/68‑1 (MQ=255) aaGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGTTTa < 1:2117098/68‑1 (MQ=255) aaGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGTTTa < 1:1221066/68‑1 (MQ=255) ccAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGTTTAACaa < 1:2189343/69‑1 (MQ=255) cAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGTTTAACAAg < 1:2823785/69‑1 (MQ=255) cAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGTTTAACAAg < 1:255903/69‑1 (MQ=255) cAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGTTTAACAAg < 1:2512908/69‑1 (MQ=255) cAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGTTTAACAAg < 1:2447866/69‑1 (MQ=255) cAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGTTTAACAAg < 1:2432022/69‑1 (MQ=255) cAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGTTTAACAAg < 1:1642470/69‑1 (MQ=255) cAAATCGATAAGCTCGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGTTTAACAAg < 1:709466/69‑1 (MQ=255) | GCGACAAAAGCCATATCTGGCTACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGTTTAACAAG > minE/2499292‑2499422 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |