| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,499,362 | T→C | 33.7% | D70D (GAT→GAC) | ilvN → | acetolactate synthase I, small subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,499,362 | 0 | T | C | 33.7% | 177.4 / 129.9 | 222 | D70D (GAT→GAC) | ilvN | acetolactate synthase I, small subunit |
| Reads supporting (aligned to +/- strand): ref base T (86/61); new base C (58/17); total (144/78) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.22e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.24e-01 | |||||||||||
Only 100 of 225 total aligned reads displayed.
CAAAAGCCATATCTGGCTACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGTTTAACAAGATCGCGGT > minE/2499296‑2499430 | cATATCTGGCTACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGaa < 1:744977/69‑1 (MQ=255) tCTGGCTACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAATCTGGAAGATg > 1:814637/1‑69 (MQ=255) tCTGGCTACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATg > 1:2187987/1‑69 (MQ=255) tCTGGCTACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATg > 1:2790313/1‑69 (MQ=255) tCTGGCTACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATg > 1:2051159/1‑69 (MQ=255) tCTGGCTACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATg > 1:2910693/1‑69 (MQ=255) tCTGGCTACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATg > 1:3171597/1‑69 (MQ=255) tCTGGCTACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATg > 1:1720936/1‑69 (MQ=255) tCTGGCTACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATg > 1:142460/1‑69 (MQ=255) cTGGCTACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGaa < 1:2462521/64‑1 (MQ=255) cTACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGat < 1:3294408/60‑2 (MQ=255) tACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAGATAGACAAGCTGGAAGATGTGGTGa < 1:835959/69‑1 (MQ=255) tACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAGATAGACAAGCTGGAAGATGTGGTGa < 1:1006113/69‑1 (MQ=255) tACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAGATAGACAAGCTGGAAGATGTGGTGa < 1:3025666/69‑1 (MQ=255) tACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAGATAGACAAGCTGGAAGATGTGGTGa < 1:672159/69‑1 (MQ=255) tACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAGATAGACAAGCTGGAAGATGTGGTGa < 1:2793565/69‑1 (MQ=255) tACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAGATAGACAAGCTGGAAGATGTGGTGa < 1:3317454/69‑1 (MQ=255) tACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAGATAGACAAGCTGGAAGATGTGGTGa < 1:3324263/69‑1 (MQ=255) tACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAGATAGACAAGCTGGAAGATGTGGTGa < 1:2193082/69‑1 (MQ=255) tACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAGATAGACAAGCTGGAAGATGTGGTGa < 1:1359355/69‑1 (MQ=255) tACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGt < 1:492931/67‑1 (MQ=255) aCTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTTGAAGATGTCGTGaa < 1:1741600/69‑1 (MQ=255) tCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGc > 1:1384922/1‑69 (MQ=255) tCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGc > 1:3396932/1‑69 (MQ=255) cAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCa < 1:2230615/69‑1 (MQ=255) cAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCa < 1:2064833/69‑1 (MQ=255) cAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCa < 1:1899250/69‑1 (MQ=255) cAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCa < 1:1222335/69‑1 (MQ=255) ccgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTa > 1:2356728/2‑69 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGGTAAGCTGGAAGATGTCGTGAAAGTGCAGCGTa > 1:2740957/1‑69 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTTGAAGATATCGTGAAAGTGCAGCGTa > 1:2927655/1‑69 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGt > 1:334877/1‑68 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGt > 1:2811313/1‑68 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGt > 1:2846169/1‑68 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGt > 1:1174072/1‑68 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGt > 1:597280/1‑68 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGt > 1:1523995/1‑68 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTa > 1:1147297/1‑69 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTa > 1:2503158/1‑69 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTa > 1:850075/1‑69 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTa > 1:1739281/1‑69 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTa > 1:1214075/1‑69 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTa > 1:1655002/1‑69 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTa > 1:1647875/1‑69 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTa > 1:236581/1‑69 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTa > 1:660391/1‑69 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTa > 1:3371889/1‑69 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTa > 1:93534/1‑69 (MQ=255) gCGTCTGGAGCAGATGATAAGCCAGATAGACAAGCTGGAAGATGTGGTGAAAGTGCAGCGTAATCAGt > 1:3094529/1‑68 (MQ=255) gCGTCTGGAGCAGATGATAAGCCAGATAGACAAGCTGGAAGATGTGGTGAAAGTGCAGCGTAATCAGt > 1:2280330/1‑68 (MQ=255) gCGTCTGGAGCAGATGATAAGCCAGATAGACAAGCTGGAAGATGTGGTGAAAGTGCAGCGTAATCAGt > 1:2018884/1‑68 (MQ=255) gCGTCTGGAGCAGATGATAAGCCAGATAGACAAGCTGGAAGATGTGGTGAAAGTGCAGCGTAATCAGt > 1:150394/1‑68 (MQ=255) tCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGtccga > 1:2312118/1‑69 (MQ=255) tCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGtccga > 1:632967/1‑69 (MQ=255) gCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCcgac < 1:1767899/69‑1 (MQ=255) gCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCcgac < 1:1854549/69‑1 (MQ=255) gCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCcgac < 1:1875404/69‑1 (MQ=255) gCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCcgac < 1:2199916/69‑1 (MQ=255) aGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCcgac < 1:1918051/67‑1 (MQ=255) gatgatAAGCCAGATAGACAAGCTGGAAGATGTGGTGa > 1:2440383/1‑38 (MQ=21) gatgatAAGCCAGATAGACAAGCTGGAAGATGTGGTGa > 1:3336750/1‑38 (MQ=21) gatgatAAGCCAGATAGACAAGCTGGAAGATGTGGTGa > 1:1499919/1‑38 (MQ=21) gatgatAAGCCAGATAGACAAGCTGGAAGATGTGGTGa > 1:3422250/1‑38 (MQ=21) gatgatAAGCCAGATAGACAAGCTGGAAGATGTGGTGa > 1:425562/1‑38 (MQ=21) gatgatAAGCCAGATAGACAAGCTGGAAGATGTGGTGa > 1:1319323/1‑38 (MQ=21) gatgatAAGCCAGATAGACAAGCTGGAAGATGTGGTGa > 1:1988921/1‑38 (MQ=21) gatgatAAGCCAGATAGACAAGCTGGAAGATGTGGTGa > 1:2624147/1‑38 (MQ=21) gatgatAAGCCAGATAGACAAGCTGGAAGATGTGGTGa > 1:1057773/1‑38 (MQ=21) gatAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGt > 1:1210174/1‑68 (MQ=255) gatAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGt > 1:899623/1‑68 (MQ=255) atAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGttt < 1:1293086/69‑1 (MQ=255) atAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGttt < 1:2962883/69‑1 (MQ=255) atAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGttt < 1:3002548/69‑1 (MQ=255) atAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGttt < 1:1165509/69‑1 (MQ=255) atAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGttt < 1:1068410/69‑1 (MQ=255) atAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGttt < 1:1538796/69‑1 (MQ=255) tAAGCCAGATAGACAAGCTGGAAGATGTGGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGttt > 1:499468/1‑68 (MQ=255) tAAGCCAGATAGACAAGCTGGAAGATGTGGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGttt > 1:1260119/1‑68 (MQ=255) tAAGCCAGATAGACAAGCTGGAAGATGTGGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGttt > 1:1673508/1‑68 (MQ=255) tAAGCCAGATAGACAAGCTGGAAGATGTGGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGttt > 1:3270343/1‑68 (MQ=255) tAAGCCAGATAGACAAGCTGGAAGATGTGGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGttt > 1:2265892/1‑68 (MQ=255) tAAGCCAGATAGACAAGCTGGAAGATGTGGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGttt > 1:180932/1‑68 (MQ=255) tAAGCCAGATAGACAAGCTGGAAGATGTGGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGttt > 1:304494/1‑68 (MQ=255) tAAGCCAGATAGACAAGCTGGAAGATGTGGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGttt > 1:2116509/1‑68 (MQ=255) tAAGCCAGATAGACAAGCTGGAAGATGTGGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGttt > 1:2662222/1‑68 (MQ=255) tAAGCCAGATAGACAAGCTGGAAGATGTGGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGttt > 1:2704786/1‑68 (MQ=255) tAAGCCAGATAGACAAGCTGGAAGATGTGGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGtt > 1:2399890/1‑67 (MQ=255) tAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGttt > 1:103787/1‑68 (MQ=255) tAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGttt > 1:1041903/1‑68 (MQ=255) tAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGttt > 1:724064/1‑68 (MQ=255) aaGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGTTTa < 1:1221066/68‑1 (MQ=255) aGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGTTTa < 1:2121378/67‑1 (MQ=255) cAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGTTTAACAAg < 1:1841657/69‑1 (MQ=255) cAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGTTTAACAAg < 1:255903/69‑1 (MQ=255) cAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGTTTAACAAg < 1:2512908/69‑1 (MQ=255) cAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGTTTAACAAg < 1:1338919/69‑1 (MQ=255) cAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGTTTAACAAg < 1:140190/69‑1 (MQ=255) cAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGTTTAACAAg < 1:2432022/69‑1 (MQ=255) cAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGTTTAACAAg < 1:1642470/69‑1 (MQ=255) cAAATCGATAAGCTCGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGTTTAACAAg < 1:709466/69‑1 (MQ=255) | CAAAAGCCATATCTGGCTACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGTTTAACAAGATCGCGGT > minE/2499296‑2499430 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |