| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,499,377 | C→G | 37.7% | V75V (GTC→GTG) | ilvN → | acetolactate synthase I, small subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,499,377 | 0 | C | G | 37.7% | 167.3 / 179.5 | 199 | V75V (GTC→GTG) | ilvN | acetolactate synthase I, small subunit |
| Reads supporting (aligned to +/- strand): ref base C (65/59); new base G (58/17); total (123/76) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.09e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 199 total aligned reads displayed.
TACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGTTTAACAAGATCGCGGTG > minE/2499313‑2499431 | tACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAGATAGACAAGCTGGAAGATGTGGTGa < 1:1006113/69‑1 (MQ=255)tACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAGATAGACAAGCTGGAAGATGTGGTGa < 1:2193082/69‑1 (MQ=255)tACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAGATAGACAAGCTGGAAGATGTGGTGa < 1:835959/69‑1 (MQ=255)tACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAGATAGACAAGCTGGAAGATGTGGTGa < 1:2285261/69‑1 (MQ=255)tACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAGATAGACAAGCTGGAAGATGTGGTGa < 1:1168638/69‑1 (MQ=255)tACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAGATAGACAAGCTGGAAGATGTGGTGa < 1:1743255/69‑1 (MQ=255)tACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAGATAGACAAGCTGGAAGATGTGGTGa < 1:3317454/69‑1 (MQ=255)tACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGt < 1:1081579/67‑1 (MQ=255) aCTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGaa < 1:2849418/69‑1 (MQ=255) tCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTg > 1:1882214/1‑68 (MQ=255) tCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGc > 1:1036387/1‑69 (MQ=255) tCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGc > 1:1504933/1‑69 (MQ=255) tCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGc > 1:730027/1‑69 (MQ=255) cAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCa < 1:1222335/69‑1 (MQ=255) cAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCa < 1:2064833/69‑1 (MQ=255) cAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCa < 1:1293823/69‑1 (MQ=255) cAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCa < 1:2230615/69‑1 (MQ=255) cAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCa < 1:134609/69‑1 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGGTAAGCTGGAAGATGTCGTGAAAGTGCAGCGTa > 1:2740957/1‑69 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGaaa > 1:3003016/1‑59 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGt > 1:623615/1‑68 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGt > 1:1194803/1‑68 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGt > 1:2792189/1‑68 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGt > 1:2794792/1‑68 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGt > 1:1626286/1‑68 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGt > 1:1526994/1‑68 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGt > 1:684430/1‑68 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGt > 1:334877/1‑68 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTa > 1:2913452/1‑69 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTa > 1:2972701/1‑69 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTa > 1:2503158/1‑69 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTa > 1:2416760/1‑69 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTa > 1:236581/1‑69 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTa > 1:667039/1‑69 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTa > 1:825426/1‑69 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTa > 1:3327822/1‑69 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTa > 1:850075/1‑69 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTa > 1:2021266/1‑69 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTa > 1:93534/1‑69 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTa > 1:171356/1‑69 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTa > 1:1649096/1‑69 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTa > 1:1214075/1‑69 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTa > 1:1234696/1‑69 (MQ=255) acgacCAGCGTCTGGAGCAGATGATAACCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTg > 1:1384873/1‑62 (MQ=255) gCGTCTGGAGCAGATGATAAGCCAGATAGACAAGCTGGAAGATGTGGTGAAAGTGCAGCGTAATCAg > 1:1664099/1‑67 (MQ=255) gCGTCTGGAGCAGATGATAAGCCAGATAGACAAGCTGGAAGATGTGGTGAAAGTGCAGCGTAATCAGt > 1:1970618/1‑68 (MQ=255) gCGTCTGGAGCAGATGATAAGCCAGATAGACAAGCTGGAAGATGTGGTGAAAGTGCAGCGTAATCAGt > 1:2623931/1‑68 (MQ=255) gCGTCTGGAGCAGATGATAAGCCAGATAGACAAGCTGGAAGATGTGGTGAAAGTGCAGCGTAATCAGt > 1:3172050/1‑68 (MQ=255) gCGTCTGGAGCAGATGATAAGCCAGATAGACAAGCTGGAAGATGTGGTGAAAGTGCAGCGTAATCAGt > 1:1741078/1‑68 (MQ=255) gCGTCTGGAGCAGATGATAAGCCAGATAGACAAGCTGGAAGATGTGGTGAAAGTGCAGCGTAATCAGt > 1:1768636/1‑68 (MQ=255) gCGTCTGGAGCAGATGATAAGCCAGATAGACAAGCTGGAAGATGTGGTGAAAGTGCAGCGTAATCAGt > 1:500434/1‑68 (MQ=255) gCGTCTGGAGCAGATGATAAGCCAGATAGACAAGCTGGAAGATGTGGTGAAAGTGCAGCGTAATCAGt > 1:643705/1‑68 (MQ=255) gCGTCTGGAGCAGATGATAAGCCAGATAGACAAGCTGGAAGATGTGGTGAAAGTGCAGCGTAATCAGt > 1:1066483/1‑68 (MQ=255) gCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCcgac < 1:2199916/69‑1 (MQ=255) gCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCcgac < 1:1854549/69‑1 (MQ=255) gCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCcgac < 1:1417309/69‑1 (MQ=255) gCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCcgac < 1:1875404/69‑1 (MQ=255) aGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCcgacga < 1:2253693/69‑1 (MQ=255) aGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCcgacga < 1:2007952/69‑1 (MQ=255) gatgatAAGCCAGATAGACAAGCTGGAAGATGTGGTGa > 1:425562/1‑38 (MQ=21) gatgatAAGCCAGATAGACAAGCTGGAAGATGTGGTGa > 1:3422250/1‑38 (MQ=21) gatgatAAGCCAGATAGACAAGCTGGAAGATGTGGTGa > 1:3336750/1‑38 (MQ=21) gatgatAAGCCAGATAGACAAGCTGGAAGATGTGGTGa > 1:1904728/1‑38 (MQ=21) gatgatAAGCCAGATAGACAAGCTGGAAGATGTGGTGa > 1:1038841/1‑38 (MQ=21) gatgatAAGCCAGATAGACAAGCTGGAAGATGTGGTGa > 1:2273730/1‑38 (MQ=21) gatgatAAGCCAGATAGACAAGCTGGAAGATGTGGTGa > 1:2440383/1‑38 (MQ=21) gatgatAAGCCAGATAGACAAGCTGGAAGATGTGGTGa > 1:1733993/1‑38 (MQ=21) gatgatAAGACAGATAGACAAGCTGGAAGATGTGGTGa > 1:1642974/1‑38 (MQ=16) tgatAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATg > 1:2181262/1‑68 (MQ=255) gatAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGt > 1:899623/1‑68 (MQ=255) gatAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGt > 1:1210174/1‑68 (MQ=255) atAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGttt < 1:1334643/69‑1 (MQ=255) atAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGttt < 1:2357912/69‑1 (MQ=255) tAAGCCAGATAGACAAGCTGGAAGATGTGGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGttt > 1:2704786/1‑68 (MQ=255) tAAGCCAGATAGACAAGCTGGAAGATGTGGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGttt > 1:498615/1‑68 (MQ=255) tAAGCCAGATAGACAAGCTGGAAGATGTGGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGttt > 1:180932/1‑68 (MQ=255) tAAGCCAGATAGACAAGCTGGAAGATGTGGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGttt > 1:3383813/1‑68 (MQ=255) tAAGCCAGATAGACAAGCTGGAAGATGTGGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGttt > 1:3270343/1‑68 (MQ=255) tAAGCCAGATAGACAAGCTGGAAGATGTGGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGttt > 1:1390414/1‑68 (MQ=255) tAAGCCAGATAGACAAGCTGGAAGATGTGGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGttt > 1:2956874/1‑68 (MQ=255) tAAGCCAGATAGACAAGCTGGAAGATGTGGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGttt > 1:1482512/1‑68 (MQ=255) tAAGCCAGATAGACAAGCTGGAAGATGTGGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGttt > 1:1501162/1‑68 (MQ=255) tAAGCCAGATAGACAAGCTGGAAGATGTGGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGttt > 1:1157473/1‑68 (MQ=255) tAAGCCAGATAGACAAGCTGGAAGATGTGGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGttt > 1:1047623/1‑68 (MQ=255) tAAGCCAGATAGACAAGCTGGAAGATGTGGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGtt > 1:3026608/1‑67 (MQ=255) tAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGttt > 1:2626278/1‑68 (MQ=255) aaGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGTTTa < 1:3062961/68‑1 (MQ=255) aaGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGTTTa < 1:2323148/68‑1 (MQ=255) aaGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGTTTa < 1:2117098/68‑1 (MQ=255) aaGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGTTTa < 1:1221066/68‑1 (MQ=255) aGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGTTTa < 1:2121378/67‑1 (MQ=255) ccAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGTTTAACaa < 1:1261536/69‑1 (MQ=255) cAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGTTTAACAAg < 1:1841657/69‑1 (MQ=255) cAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGTTTAACAAg < 1:3415921/69‑1 (MQ=255) cAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGTTTAACAAg < 1:2823785/69‑1 (MQ=255) cAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGTTTAACAAg < 1:2447866/69‑1 (MQ=255) cAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGTTTAACAAg < 1:255903/69‑1 (MQ=255) cAAATCGATAAGCTCGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGTTTAACAAg < 1:709466/69‑1 (MQ=255) aGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGTTTAACAAGATCGCGgt < 1:2046449/67‑1 (MQ=255) tCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGTTTa < 1:2079740/42‑1 (MQ=255) | TACTGGTCAATGACGACCAGCGTCTGGAGCAGATGATAAGCCAAATCGATAAGCTGGAAGATGTCGTGAAAGTGCAGCGTAATCAGTCCGATCCGACGATGTTTAACAAGATCGCGGTG > minE/2499313‑2499431 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |