| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,499,815 | T→C | 34.2% | L99L (CTT→CTC) | uhpA → | DNA‑binding response regulator in two‑component regulatory system wtih UhpB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,499,815 | 0 | T | C | 34.2% | 21.1 / 16.3 | 35 | L99L (CTT→CTC) | uhpA | DNA‑binding response regulator in two‑component regulatory system wtih UhpB |
| Reads supporting (aligned to +/- strand): ref base T (21/2); new base C (3/9); total (24/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.37e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTCCGTTCACGACAGTCCTGCGCTGGTTGAGCAGGCGCTTAACGCGGGGGCACGCGGCTTTCTTTCCAAACGCTGTAGCCCGGATGAACTCATTGCTGCGGTGCATACGGTTGCCACGGGCGGCTGTTAT > minE/2499752‑2499881 | ctcCGTTCACGACAGTCCTGCGCTTGTTGAGCAGGCGCTCAACGCGGGGGCACGCGGCTTTCTTTCCaa > 1:1658479/1‑69 (MQ=255)ctcCGTTCACGACAGTCCTGCGCTGGTTGAGCAGGCGCTTAACGCGGGGGCACGCGGCTTTCTTTCCaa > 1:898698/1‑69 (MQ=255)ctcCGTTCACGACAGTCCTGCGCTGGTTGAGCAGGCGCTTAACGCGGGGGCACGCGGCTTTCTTTCCaa > 1:333084/1‑69 (MQ=255)ctcCGTTCACGACAGTCCTGCGCTGGTTGAGCAGGCGCTTAACGCGGGGGCACGCGGCTTTCTTTCCaa > 1:126139/1‑69 (MQ=255)ctcCGTTCACGACAGTCCTGCGCTGGTTGAGCAGGCGCTTAACGCGGGGGCACGCGGCTTTCTTTCCaa > 1:1635948/1‑69 (MQ=255)ctcCGTTCACGACAGTCCTGCGCTGGTTGAGCAGGCGCTTAACGCGGGGGCACGCGGCTTTCTTTCCa < 1:231761/68‑1 (MQ=255)ctcCGTTCACGACAGTCCTGCGCTGGTTGAGCAGGCGCTTAACGCGGGGGCACGCGGCTTTCTTTCCa > 1:2375140/1‑68 (MQ=255)ctcCGTTCACGACAGTCCTGCGCTGGTTGAGCAGGCGCTTAACGCGGGGGCACGCGGCTTTCTTTCCa > 1:23437/1‑68 (MQ=255) cACGACAGTCCTGCGCTGGTTGAGCAGGCGCTTAACGCGGGGGCACGCGGCTTTCTTTCCAAACGCTGt > 1:1465106/1‑69 (MQ=255) cACGACAGTCCTGCGCTGGTTGAGCAGGCGCTTAACGCGGGGGCACGCGGCTTTCTTTCCAAACGCTGt > 1:835612/1‑69 (MQ=255) cACGACAGTCCTGCGCTGGTTGAGCAGGCGCTTAACGCGGGGGCACGCGGCTTTCTTTCCAAACGCTGt > 1:3341615/1‑69 (MQ=255) cACGACAGTCCTGCGCTGGTTGAGCAGGCGCTTAACGCGGGGGCACGCGGCTTTCTTTCCAAACGCTGt > 1:3032445/1‑69 (MQ=255) cACGACAGTCCTGCGCTGGTTGAGCAGGCGCTTAACGCGGGGGCACGCGGCTTTCTTTCCAAACGCTGt > 1:296544/1‑69 (MQ=255) cACGACAGTCCTGCGCTGGTTGAGCAGGCGCTTAACGCGGGGGCACGCGGCTTTCTTTCCAAACGCTGt > 1:2788761/1‑69 (MQ=255) cACGACAGTCCTGCGCTGGTTGAGCAGGCGCTTAACGCGGGGGCACGCGGCTTTCTTTCCAAACGCTGt > 1:2623021/1‑69 (MQ=255) cACGACAGTCCTGCGCTGGTTGAGCAGGCGCTTAACGCGGGGGCACGCGGCTTTCTTTCCAAACGCTGt > 1:2394510/1‑69 (MQ=255) cACGACAGTCCTGCGCTGGTTGAGCAGGCGCTTAACGCGGGGGCACGCGGCTTTCTTTCCAAACGCTGt > 1:2366145/1‑69 (MQ=255) cACGACAGTCCTGCGCTGGTTGAGCAGGCGCTTAACGCGGGGGCACGCGGCTTTCTTTCCAAACGCTGt > 1:2201521/1‑69 (MQ=255) cACGACAGTCCTGCGCTGGTTGAGCAGGCGCTTAACGCGGGGGCACGCGGCTTTCTTTCCAAACGCTGt > 1:1269862/1‑69 (MQ=255) cACGACAGTCCTGCGCTGGTTGAGCAGGCGCTTAACGCGGGGGCACGCGGCTTTCTTTCCAAACGCTGt > 1:1918663/1‑69 (MQ=255) aGTCCGGCGCTGGTTGAGCAGGCGCTTAACGCGGGGGCGCGCGGCTTTCTCTCCAAACGCTGTAGccc < 1:1938976/68‑1 (MQ=255) aGTCCGGCGCTGGTTGAGCAGGCGCTTAACGCGGGGGCGCGCGGCTTTCTCTCCAAACGCTGTAGccc < 1:1416488/68‑1 (MQ=255) aGTCCGGCGCTGGTTGAGCAGGCGCTTAACGCGGGGGCGCGCGGCTTTCTCTCCAAACGCTGTAGccc < 1:2877145/68‑1 (MQ=255) aGTCCGGCGCTGGTTGAGCAGGCGCTTAACGCGGGGGCGCGCGGCTTTCTCTCCAAACGCTGTAGccc < 1:2662021/68‑1 (MQ=255) aGTCCGGCGCTGGTTGAGCAGGCGCTTAACGCGGGGGCGCGCGGCTTTCTCTCCAAACGCTGTAGccc < 1:1771278/68‑1 (MQ=255) aGTCCGGCGCTGGTTGAGCAGGCGCTTAACGCGGGGGCGCGCGGCTTTCTCTCCAAACGCTGTAGccc < 1:1777837/68‑1 (MQ=255) aGTCCGGCGCTGGTTGAGCAGGCGCTTAACGCGGGGGCGCGCGGCTTTCTCTCCAAACGCTGTAGccc < 1:1433850/68‑1 (MQ=255) aGTCCGGCGCTGGTTGAGCAGGCGCTTAACGCGGGGGCGCGCGGCTTTCTCTCCAAACGCTGTAGccc < 1:2167085/68‑1 (MQ=255) aGTCCGGCGCTGGTTGAGCAGGCGCTTAACGCGGGGGCGCGCGGCTTTCTCTCCAAACGCTGTAGccc < 1:1944918/68‑1 (MQ=255) ccTGCGCTGGTTGAGCAGGCGCTTAACGCGGGGGCACGCGGCTTTCTTTCCAAACGCTGTAGCCCGGa > 1:3379186/1‑68 (MQ=255) ccTGCGCTGGTTGAGCAGGCGCTTAACGCGGGGGCACGCGGCTTTCTTTCCAAACGCTGTAGCCCGGa > 1:3081793/1‑68 (MQ=255) ggTTGAGCAGGCGCTTAACGCGGGGGCGCGCGGCTTTCTCTCCAAACGCTGTAGCCCGGATGAACTCAt > 1:1063870/1‑69 (MQ=255) ggTTGAGCAGGCGCTTAACGCGGGGGCGCGCGGCTTTCTCTCCAAACGCTGTAGCCCGGATGAACTCAt > 1:510101/1‑69 (MQ=255) ggTTGAGCAGGCGCTTAACGCGGGGGCGCGCGGCTTTCTCTCCAAACGCTGTAGCCCGGATGAACTCAt > 1:1948247/1‑69 (MQ=255) gggggCACGCGGCTTTCTTTCCAAACGCTGTAGCCCGGATGAACTCATTGCTGCGGTGCATACGGTTGc < 1:740299/69‑1 (MQ=255) tttctttcCAAACGCTGTAGCCCGGATGAACTCATTGCTGCGGTGCATACGGTTGCCACGGGCGGCTg > 1:224441/1‑68 (MQ=255) ttctttcCAAACGCTGTAGCCCGGATGAACTCATTGCTGCGGTGCATACGGTTTCCACGggcg < 1:2691433/63‑1 (MQ=255) ttctttcCAAACGCTGTAGCCCGGATGAACTCATTGCTGCGGTGCATACGGTTGCCACGggcg < 1:2041151/63‑1 (MQ=255) ttctttcCAAACGCTGTAGCCCGGATGAACTCATTGCTGCGGTGCATACGGTTGCCACGggcg < 1:1505528/63‑1 (MQ=255) ttctttcCAAACGCTGTAGCCCGGATGAACTCATTGCTGCGGTGCATACGGTTGCCACGGGCGGCTGtt < 1:1938806/69‑1 (MQ=255) ttctttcCAAACGCTGTAGCCCGGATGAACTCATTGCTGCGGTGCATACGGTTGCCACGGGCGGCTGtt < 1:1960439/69‑1 (MQ=255) ttctttcCAAACGCTGTAGCCCGGATGAACTCATTGCTGCGGTGCATACGGTTGCCACGGGCGGCTGtt < 1:3153254/69‑1 (MQ=255) ttctttcCAAACGCTGTAGCCCGGATGAACTCATTGCTGCGGTGCATACGGTTGCCACGGGCGGCTGtt < 1:3265920/69‑1 (MQ=255) ttctttcCAAACGCTGTAGCCCGGATGAACTCATTGCTGCGGTGCATACGGTTGCCACGGGCGGCTGtt > 1:1195177/1‑69 (MQ=255) ttctttcCAAACGCTGTAGCCCGGATGAACTCATTGCTGCGGTGCATACGGTTGCCACGGGCGGCTGtt < 1:2418888/69‑1 (MQ=255) ttctttcCAAACGCTGTAGCCCGGATGAACTCATTGCTGCGGTGCATACGGTTGCCACGGGCGGCTGtt < 1:3432070/69‑1 (MQ=255) ttctttcCAAACGCTGTAGCCCGGATGAACTCATTGCTGCGGTGCATACGGTTGCCACGGGCGGCTGtt < 1:542678/69‑1 (MQ=255) ttctttcCAAACGCTGTAGCCCGGATGAACTCATTGCTGCGGTGCATACGGTTGCCACGGGCGGCTGtt < 1:769468/69‑1 (MQ=255) ttctttcCAAACGCTGTAGCCCGGATGAACTCATTGCTGCGGTGCATACGGTTGCCACGGGCGGCTGt > 1:458201/1‑68 (MQ=255) ctttcCAAACGCTGTAGCCCGGATGAACTCATTGCTGCGGTGCATACGGtt < 1:1233700/51‑1 (MQ=255) ctttcAAAACGCTGTAGCCCGGATGAACTCATTGCTGCGGTGCATACGGtt < 1:1733285/51‑1 (MQ=255) tttcCAAACGCTGTAGCCCGGATGAACTCATTGCTGCGGTGCATACGGTTGCCACGGGCGGCTGTTa < 1:1892741/67‑1 (MQ=255) tttcCAAACGCTGTAGCCCGGATGAACTCATTGCTGCGGTGCATACGGTTGCCACGGGCGGCTGTTa < 1:1246333/67‑1 (MQ=255) tttcCAAACGCTGTAGCCCGGATGAACTCATTGCTGCGGTGCATACGGTTGCCACGGGCGGCTGTTAt < 1:1080158/68‑1 (MQ=255) | CTCCGTTCACGACAGTCCTGCGCTGGTTGAGCAGGCGCTTAACGCGGGGGCACGCGGCTTTCTTTCCAAACGCTGTAGCCCGGATGAACTCATTGCTGCGGTGCATACGGTTGCCACGGGCGGCTGTTAT > minE/2499752‑2499881 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |