| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,503,335 | C→T | 18.9% | S86S (TCC→TCT) | uhpT → | hexose phosphate transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,503,335 | 0 | C | T | 18.9% | 96.8 / 12.1 | 63 | S86S (TCC→TCT) | uhpT | hexose phosphate transporter |
| Reads supporting (aligned to +/- strand): ref base C (35/16); new base T (5/7); total (40/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.03e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01 | |||||||||||
ATCGGCCTGGGTTTCTCCATCACTTATGGCGTGGGTAAAACGCTGGTTTCCTACTACGCCGACGGCAAAAACACCAAACAATTCCTGCCGTTCATGCTGATCCTCTCTGCTATTTG > minE/2503285‑2503400 | atCGGCCTGGGTTTCTCCATCACTTATGGCGTGGGTAAAACGCTGGTTTCCTACTACGCCGACGGCaaa > 1:36433/1‑69 (MQ=255)atCGGCCTGGGTTTCTCCATCACTTATGGCGTGGGTAAAACGCTGGTTTCCTACTACGCCGACGGCaaa > 1:1475302/1‑69 (MQ=255)atCGGCCTGGGCTTCTCCATCACTTATGGCGTGGGTAAAACGCTGGTTTCTTACTACGCCGACGGCaaa < 1:2965516/69‑1 (MQ=255)atCGGCCTGGGCTTCTCCATCACTTATGGCGTGGGTAAAACGCTGGTTTCTTACTACGCCGACGGCaaa < 1:2566074/69‑1 (MQ=255)atCGGCCTGGGCTTCTCCATCACTTATGGCGTGGGTAAAACGCTGGTTTCTTACTACGCCGACGGCaaa < 1:2635314/69‑1 (MQ=255)atCGGCCTGGGCTTCTCCATCACTTATGGCGTGGGTAAAACGCTGGTTTCTTACTACGCCGACGGCaaa < 1:2213191/69‑1 (MQ=255)atCGGCCTGGGCTTCTCCATCACTTATGGCGTGGGTAAAACGCTGGTTTCTTACTACGCCGACGGCaaa < 1:1791729/69‑1 (MQ=255)atCGGCCTGGGCTTCTCCATCACTTATGGCGTGGGTAAAACGCTGGTTTCTTACTACGCCGACGGCaaa < 1:3190942/69‑1 (MQ=255)atCGGCCTGGGCTTCTCCATCACTTATGGCGTGGGTAAAACGCTGGTTTCTTACTACGCCGACGGCaaa < 1:1352500/69‑1 (MQ=255) gTTTCTCCATCACTTATGGCGTGGGTAAAACGCTGGTTTCCTACTACGCCGACGGCAAAAACACCAAAc > 1:147030/1‑69 (MQ=255) tttCTCCATCACTTATGGCGTGGGTAAAACGCTGGTTTCCTACTACGCCGACGGCAAAAACACCAAAc > 1:1474364/1‑68 (MQ=255) tttCTCCATCACTTATGGCGTGGGTAAAACGCTGGTTTCCTACTACGCCGACGGCAAAAACACCAAACa > 1:170422/1‑69 (MQ=255) tttCTCCATCACTTATGGCGTGGGTAAAACGCTGGTTTCCTACTACGCCGACGGCAAAAACACCAAACa > 1:2994153/1‑69 (MQ=255) tttCTCCATCACTTATGGCGTGGGTAAAACGCTGGTTTCCTACTACGCCGACGGCAAAAACACCAAACa > 1:2051361/1‑69 (MQ=255) tttCTCCATCACTTATGGCGTGGGTAAAACGCTGGTTTCCTACTACGCCGACGGCAAAAACACCAAACa > 1:2169781/1‑69 (MQ=255) ttCTCCATCACTTATGGCGTGGGTAAAACGCTGGTTTCCTACTACGCCGACGGCAAAAACACCAAACaa < 1:573969/69‑1 (MQ=255) ttCTCCATCACTTATGGCGTGGGTAAAACGCTGGTTTCCTACTACGCCGACGGCAAAAACACCAAACaa < 1:2492326/69‑1 (MQ=255) ttCTCCATCACTTATGGCGTGGGTAAAACGCTGGTTTCCTACTACGCCGACGGCAAAAACACCAAACaa < 1:320647/69‑1 (MQ=255) ttCTCCATCACTTATGGCGTGGGTAAAACGCTGGTTTCCTACTACGCCGACGGCAAAAACACCAAACaa < 1:2192969/69‑1 (MQ=255) ttCTCCATCACTTATGGCGTGGGTAAAACGCTGGTTTCCTACTACGCCGACGGCAAAAACACCAAACaa < 1:2002439/69‑1 (MQ=255) tcCATCACTTATGGCGTGGGTAAAACGCTGGTTTCCTACTACGCCGACGGCAAAAACACCAAACAATTc > 1:3148975/1‑69 (MQ=255) aCTTATGGCGTGGGTAAAACGCTGGTTTCCTACTACGCCGACGGCaaaaa > 1:2739477/1‑50 (MQ=255) aCTTATGGCGTGGGTAAAACGCTGGTTTCCTACTACGCCGACGGCaaaaa > 1:593047/1‑50 (MQ=255) aCTTATGGCGTGGGTAAAACGCTGGTTTCCTACTACGCCGACGGCaaaaa > 1:2655966/1‑50 (MQ=255) aCTTATGGCGTGGGTAAAACGCTGGTTTCCTACTACGCCGACGGCaaaaa > 1:3005377/1‑50 (MQ=255) aCTTATGGCGTGGGTAAAACGCTGGTTTCCTACTACGCCGACGGCAAAAACACCAAACAATTCCTGcc > 1:631007/1‑68 (MQ=255) aCTTATGGCGTGGGTAAAACGCTGGTTTCCTACTACGCCGACGGCAAAAACACCAAACAATTCCTGcc > 1:3052161/1‑68 (MQ=255) aCTTATGGCGTGGGTAAAACGCTGGTTTCCTACTACGCCGACGGCAAAAACACCAAACAATTCCTGcc > 1:2578224/1‑68 (MQ=255) aCTTATGGCGTGGGTAAAACGCTGGTTTCCTACTACGCCGACGGCAAAAACACCAAACAATTCCTGCCg > 1:61609/1‑69 (MQ=255) aCTTATGGCGTGGGTAAAACGCTGGTTTCCTACTACGCCGACGGCAAAAACACCAAACAATTCCTGCCg > 1:864829/1‑69 (MQ=255) aCTTATGGCGTGGGTAAAACGCTGGTTTCCTACTACGCCGACGGCAAAAACACCAAACAATTCCTGCCg > 1:3054337/1‑69 (MQ=255) aCTTATGGCGTGGGTAAAACGCTGGTTTCCTACTACGCCGACGGCAAAAACACCAAACAATTCCTGCCg > 1:2631822/1‑69 (MQ=255) aCTTATGGCGTGGGTAAAACGCTGGTTTCCTACTACGCCGACGGCAAAAACACCAAACAATTCCTGCCg > 1:1559957/1‑69 (MQ=255) aTGGCGTGGGTAAAACGCTGGTTTCCTACTACGCCGACGGCAAAAACACCAAACAATTCCTGCCGTTCa > 1:1402881/1‑69 (MQ=255) aTGGCGTGGGTAAAACGCTGGTTTCCTACTACGCCGACGGCAAAAACACCAAACAATTCCTGCCGTTCa > 1:206759/1‑69 (MQ=255) aTGGCGTGGGTAAAACGCTGGTTTCCTACTACGCCGACGGCAAAAACACCAAACAATTCCTGCCGTTCa > 1:16426/1‑69 (MQ=255) gttAAAACGCTGGTTTCCTACTACGCCGACGGCAAAAACACCAAACAATTCCTGCCGTTCATGCTGATc > 1:2133929/3‑69 (MQ=255) ggTAAAACGCTGGTTTCCTACTACGCCGACGGCAAAAACACCAAACAATTCCTGCCGTTCATGCTGATc > 1:3420621/1‑69 (MQ=255) ggTAAAACGCTGGTTTCCTACTACGCCGACGGCAAAAACACCAAACAATTCCTGCCGTTCATGCTGATc > 1:184432/1‑69 (MQ=255) aaaaCGCTGGTTTCCTACTACGCCGACGGCAAAAACACCAAACAAt < 1:2431637/46‑1 (MQ=255) aaaaCGCTGGTTTCCTACTACGCCGACGGCAAAAACACCAAACAAt < 1:1478733/46‑1 (MQ=255) aaaaCGCTGGTTTCCTACTACGCCGACGGCAAAAACACCAAACAAt < 1:1482545/46‑1 (MQ=255) aaaaCGCTGGTTTCCTACTACGCCGACGGCAAAAACACCAAACAAt < 1:1594428/46‑1 (MQ=255) aaaaCGCTGGTTTCCTACTACGCCGACGGCAAAAACACCAAACAAt < 1:2387517/46‑1 (MQ=255) aaaaCGCTGGTTTCCTACTACGCCGACGGCAAAAACACCAAACAAt < 1:1092998/46‑1 (MQ=255) aaaaCGCTGGTTTCCTACTACGCCGACGGCAAAAACACCAAACAATTCCTGCCGTTCATGCt > 1:1825736/1‑62 (MQ=255) aaaaCGCTGGTTTCCTACTACGCCGACGGCAAAAACACCAAACAATTCCTGCCGTTCATGCt > 1:742200/1‑62 (MQ=255) aaaaCGCTGGTTTCCTACTACGCCGACGGCAAAAACACCAAACAATTCCTGCCGTTCATGCt > 1:779059/1‑62 (MQ=255) aaaaCGCTGGTTTCCTACTACGCCGACGGCAAAAACACCAAACAATTCCTGCCGTTCATGCt > 1:2899145/1‑62 (MQ=255) aaaaCGCTGGTTTCCTACTACGCCGACGGCAAAAACACCAAACAATTCCTGCCGTTCATGCt > 1:2843689/1‑62 (MQ=255) aaaaCGCTGGTTTCCTACTACGCCGACGGCAAAAACACCAAACAATTCCTGCCGTTCATGCt > 1:516988/1‑62 (MQ=255) aaaaCGCTGGTTTCCTACTACGCCGACGGCAAAAACACCAAACAATTCCTGCCGTTCATGCt > 1:2799907/1‑62 (MQ=255) cTGGTTTCTTACTACGCCGACGGCAAAAACACCAAAc > 1:472988/1‑37 (MQ=255) cTGGTTTCTTACTACGCCGACGGCAAAAACACCAAAc > 1:2615368/1‑37 (MQ=255) cTGGTTTCTTACTACGCCGACGGCAAAAACACCAAAc > 1:3158629/1‑37 (MQ=255) cTGGTTTCTTACTACGCCGACGGCAAAAACACCAAAc > 1:2921266/1‑37 (MQ=255) cTGGTTTCTTACTACGCCGACGGCAAAAACACCAAAc > 1:191625/1‑37 (MQ=255) ttCCTACTACGCCGACGGCAAAAACACCAAACAATTCCTGCCGTTCATg > 1:2797926/1‑49 (MQ=255) ttCCTACTACGCCGACGGCAAAAACACCAAACAATTCCTGCCGTTCATGCTGATCCTCTCTGCTATTTg < 1:3199656/69‑1 (MQ=255) ttCCTACTACGCCGACGGCAAAAACACCAAACAATTCCTGCCGTTCATGCTGATCCTCTCTGCTATTTg < 1:3079874/69‑1 (MQ=255) ttCCTACTACGCCGACGGCAAAAACACCAAACAATTCCTGCCGTTCATGCTGATCCTCTCTGCTATTTg < 1:2311137/69‑1 (MQ=255) ttCCTACTACGCCGACGGCAAAAACACCAAACAATTCCTGCCGTTCATGCTGATCCTCTCTGCTATTTg < 1:1590474/69‑1 (MQ=255) ttCCTACTACGCCGACGGCAAAAACACCAAACAATTCCTGCCGTTCATGCTGATCCTCTCTGCTATTTg < 1:1192727/69‑1 (MQ=255) | ATCGGCCTGGGTTTCTCCATCACTTATGGCGTGGGTAAAACGCTGGTTTCCTACTACGCCGACGGCAAAAACACCAAACAATTCCTGCCGTTCATGCTGATCCTCTCTGCTATTTG > minE/2503285‑2503400 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |