| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 213,289 | A→G | 37.7% | A106A (GCT→GCC) | gloB ← | predicted hydroxyacylglutathione hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 213,289 | 0 | A | G | 37.7% | 66.6 / 80.9 | 122 | A106A (GCT→GCC) | gloB | predicted hydroxyacylglutathione hydrolase |
| Reads supporting (aligned to +/- strand): ref base A (33/43); new base G (14/32); total (47/75) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 122 total aligned reads displayed.
GTCGCCGCAAAATAGATAAGGTTTACTGAAGTAACAGATATGTCCTAAAGTGTGACCCGGCGTAGCAATTACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTG > minE/213226‑213350 | gtCGCCGCAAAATAGATAAGGTTTACTGAAGTAACAGATATGTCCTAAAGTGTGACCCGGTGTGGCAAt < 1:2854540/69‑1 (MQ=255)gtCGCCGCAAAATAGATAAGGTTTACTGAAGTAACAGATATGTCCTAAAGTGTGACCCGGTGTGGCAAt < 1:1877193/69‑1 (MQ=255)gtCGCCGCAAAATAGATAAGGTTTACTGAAGTAACAGATATGTCCTAAAGTGTGACCCGGTGTGGCAAt < 1:2995224/69‑1 (MQ=255)gtCGCCGCAAAATAGATAAGGTTTACTGAAGTAACAGATATGTCCTAAAGTGTGACCCGGTGTGGCAAt < 1:1854344/69‑1 (MQ=255)gtCGCCGCAAAATAGATAAGGTTTACTGAAGTAACAGATATGTCCTAAAGTGTGACCCGGTGTGGCAAt < 1:1892329/69‑1 (MQ=255)gtCGCCGCAAAATAGATAAGGTTTACTGAAGTAACAGATATGTCCTAAAGTGTGACCCGGTGTGGCAAt < 1:3211526/69‑1 (MQ=255)gtCGCCGCAAAATAGATAAGGTTTACTGAAGTAACAGATATGTCCTAAAGTGTGACCCGGTGTGGCAAt < 1:2858192/69‑1 (MQ=255)gtCGCCGCAAAATAGATAAGGTTTACTGAAGTAACAGATATGTCCTAAAGTGTGACCCGGTGTGGCAAt < 1:3430543/69‑1 (MQ=255)gtCGCCGCAAAATAGATAAGGTTTACTGAAGTAACAGATATGTCCTAAAGTGTGACCCGGTGTGGCAAt < 1:352094/69‑1 (MQ=255)gtCGCCGCAAAATAGATAAGGTTTACTGAAGTAACAGATATGTCCTAAAGTGTGACCCGGTGTGGCAAt < 1:513399/69‑1 (MQ=255)gtCGCCGCAAAATAGATAAGGTTTACTGAAGTAACAGATATGTCCTAAAGTGTGACCCGGTGTGGCAAt < 1:2016364/69‑1 (MQ=255)gtCGCCGCAAAATAGATAAGGTTTACTGAAGTAACAGATATGTCCTAAAGTGTGACCCGGTGTGGCAAt < 1:261859/69‑1 (MQ=255)gtCGCCGCAAAATAGATAAGGTTTACTGAAGTAACAGATATGTCCTAAAGTGTGACCCGGTGTGGCAAt < 1:2269792/69‑1 (MQ=255)gtCGCCGCAAAATAGATAAGGTTTACTGAAGTAACAGATATGTCCTAAAGTGTGACCCGGTGTGGCAAt < 1:939289/69‑1 (MQ=255)gtCGCCGCAAAATAGATAAGGTTTACTGAAGTAACAGATATGTCCTAAAGTGTGACCCGGTGTGGCAAt < 1:2219189/69‑1 (MQ=255)gtCGCCGCAAAATAGATAAGGTTTACTGAAGTAACAGATATGTCCTAAAGTGTGACCCGGTGTGGCAAt < 1:869444/69‑1 (MQ=255)gtCGCCGCAAAATAGATAAGGTTTACTGAAGTAACAGATATGTCCTAAAGTGTGACCCGGTGTGGCAAt < 1:139435/69‑1 (MQ=255)gtCGCCGCAAAATAGATAAGGTTTACTGAAGTAACAGATATGTCCTAAAGTGTGACCCGGTGTGGCAAt < 1:1237831/69‑1 (MQ=255)gtCGCCGCAAAATAGATAAGGTTTACTGAAGTAACAGATATGTCCTAAAGTGTGACCCGGTGTGGCAAt < 1:1390820/69‑1 (MQ=255) tCGCCGCAAAATAGATAAGGTTTACTGAAGTAACAGATATGTCCTAAAGTGTGACCCGGTGTGGCAAt < 1:3165921/68‑1 (MQ=255) tCGCCGCAAAATAGATAAGGTTTACTGAAGTAACAGATATGTCCTAAAGTGTGACCCGGTGTGGCAAt < 1:663259/68‑1 (MQ=255) tCGCCGCAAAATAGATAAGGTTTACTGAAGTAACAGATATGTCCTAAAGTGTGACCCGGCGTAGCAAt > 1:2625886/1‑68 (MQ=255) tCGCCGCAAAATAGATAAGGTTTACTGAAGTAACAGATATGTCCTAAAGTGTGACCCGGCGTAGCAAt < 1:374862/68‑1 (MQ=255) tCGCCGCAAAATAGATAAGGTTTACTGAAGTAACAGATATGTCCTAAAGTGTGACCCGGCGTAGCAAt < 1:687220/68‑1 (MQ=255) tCGCCGCAAAATAGATAAGGTTTACTGAAGTAACAGATATGTCCTAAAGTGTGACCCGGCGTAGCAAt < 1:3347977/68‑1 (MQ=255) tCGCCGCAAAATAGATAAGGTTTACTGAAGTAACAGATATGTCCTAAAGTGTGACCCGGCGTAGCAAt < 1:3226618/68‑1 (MQ=255) tCGCCGCAAAATAGATAAGGTTTACTGAAGTAACAGATATGTCCTAAAGTGTGACCCGGCGTAGCAAt < 1:1444081/68‑1 (MQ=255) tCGCCGCAAAATAGATAAGGTTTACTGAAGTAACAGATATGTCCTAAAGTGTGACCCGGCGTAGCAAt > 1:3105265/1‑68 (MQ=255) tCGCCGCAAAATAGATAAGGTTTACTGAAGTAACAGATATGTCCTAAAGTGTGACCCGGCGTAGCAAt > 1:901508/1‑68 (MQ=255) aaaaTAGATAAGGTTTACTGAAGTAACAGATATGTCCTAAAGTGTGACCCGGTGTGGCAAt < 1:2478439/61‑1 (MQ=255) aTAGATAAGGTTTACTGAAGTAACAGATATGTCCTAAAGTGTGACCCGGCGTAGCAATTACACTAAAtt < 1:3377925/69‑1 (MQ=255) aTAGATAAGGTTTACTGAAGTAACAGATATGTCCTAAAGTGTGACCCGGCGTAGCAATTACACTAAAtt < 1:1999/69‑1 (MQ=255) aTAGATAAGGTTTACTGAAGTAACAGATATGTCCTAAAGTGTGACCCGGCGTAGCAATTACACTAAAtt < 1:1417819/69‑1 (MQ=255) aTAGATAAGGTTTACTGAAGTAACAGATATGTCCTAAAGTGTGACCCGGCGTAGCAATTACACTAAAtt < 1:781005/69‑1 (MQ=255) aTAGATAAGGTTTACTGAAGTAACAGATATGTCCTAAAGTGTGACCCGGCGTAGCAATTACACTAAAtt < 1:2587414/69‑1 (MQ=255) aTAGATAAGGTTTACTGAAGTAACAGATATGTCCTAAAGTGTGACCCGGCGTAGCAATTACACTAAAtt < 1:256903/69‑1 (MQ=255) tAAGGTTTACTGAAGTAACAGATATGTCCTAAAGTGTGACCCGGCGTAGCAATTACACTAAATTCATGc < 1:18272/69‑1 (MQ=255) tAAGGTTTACTGAAGTAACAGATATGTCCTAAAGTGTGACCCGGCGTAGCAATTACACTAAATTCATGc < 1:2776680/69‑1 (MQ=255) gTAACAGATATGTCCTAAAGTGTGACCCGGCGTAGCAATTACACTAAATTCATGCCCCAAAACGAAGGc < 1:2939728/69‑1 (MQ=255) gTAACAGATATGTCCTAAAGTGTGACCCGGCGTAGCAATTACACTAAATTCATGCCCCAAAACGAAGGc < 1:581964/69‑1 (MQ=255) gTAACAGATATGTCCTAAAGTGTGACCCGGCGTAGCAATTACACTAAATTCATGCCCCAAAACGAAGGc < 1:846769/69‑1 (MQ=255) gTAACAGATATGTCCTAAAGTGTGACCCGGCGTAGCAATTACACTAAATTCATGCCCCAAAACGAAGGc < 1:879721/69‑1 (MQ=255) gTAACAGATATGTCCTAAAGTGTGACCCGGCGTAGCAATTACACTAAATTCATGCCCCAAAACGAAGGc < 1:944116/69‑1 (MQ=255) gTAACAGATATGTCCTAAAGTGTGACCCGGCGTAGCAATTACACTAAATTCATGCCCCAAAACGAAGGc < 1:982235/69‑1 (MQ=255) gTAACAGATATGTCCTAAAGTGTGACCCGGCGTAGCAATTACACTAAATTCATGCCCCAAAACGAAGGc < 1:2127742/69‑1 (MQ=255) gTAACAGATATGTCCTAAAGTGTGACCCGGCGTAGCAATTACACTAAATTCATGCCCCAAAACGAAGGc < 1:1216641/69‑1 (MQ=255) gTAACAGATATGTCCTAAAGTGTGACCCGGCGTAGCAATTACACTAAATTCATGCCCCAAAACGAAGGc < 1:1420368/69‑1 (MQ=255) gTAACAGATATGTCCTAAAGTGTGACCCGGCGTAGCAATTACACTAAATTCATGCCCCAAAACGAAGGc < 1:2013774/69‑1 (MQ=255) tAACAGATATGTCCTAAAGTGTGACCCGGCGTAGCAATTACACTAAATTCATGCCCCAAAACGAAGGc < 1:2958135/68‑1 (MQ=255) aaCAGATATGTCCTAAAGTGTGACCCGGCGTAGCAATTACACTAAAtt > 1:1678736/1‑48 (MQ=255) aaCAGATATGTCCTAAAGTGTGACCCGGCGTAGCAATTACACTAAAtt > 1:1234077/1‑48 (MQ=255) aaCAGATATGTCCTAAAGTGTGACCCGGCGTAGCAATTACACTAAAtt > 1:3263736/1‑48 (MQ=255) aaCAGATATGTCCTAAAGTGTGACCCGGCGTAGCAATTACACTAAAtt > 1:1761489/1‑48 (MQ=255) aaCAGATATGTCCTAAAGTGTGACCCGGCGTAGCAATTACACTAAAtt > 1:1800605/1‑48 (MQ=255) aaCAGATATGTCCTAAAGTGTGACCCGGCGTAGCAATTACACTAAAtt > 1:1503437/1‑48 (MQ=255) aaCAGATATGTCCTAAAGTGTGACCCGGCGTAGCAATTACACTAAAtt > 1:1204358/1‑48 (MQ=255) aaCAGATATGTCCTAAAGTGTGACCCGGCGTAGCAATTACACTAAAtt > 1:1996315/1‑48 (MQ=255) aaCAGATATGTCCTAAAGTGTGACCCGGCGTAGCAATTACACTAAAtt > 1:2846476/1‑48 (MQ=255) aaCAGATATGTCCTAAAGTGTGACCCGGCGTAGCAATTACACTAAAtt > 1:1503916/1‑48 (MQ=255) aaCAGATATGTCCTAAAGTGTGACCCGGCGAAGCAAtt > 1:1873090/1‑38 (MQ=255) aCAGATATGTCCTAAAGTGTGACCCGGTGTGTCAATTACACTAAATTCATGCCCCAAAACGAAGGCAGt < 1:3399128/69‑1 (MQ=255) aCAGATATGTCCTAAAGTGTGACCCGGTGTGGCAATTACACTAAATTCATGCCCCAAAACGAAGGCAGt < 1:1237782/69‑1 (MQ=255) aCAGATATGTCCTAAAGTGTGACCCGGTGTGGCAATTACACTAAATTCATGCCCCAAAACGAAGGCAGt < 1:1484589/69‑1 (MQ=255) aCAGATATGTCCTAAAGTGTGACCCGGTGTGGCAATTACACTAAATTCATGCCCCAAAACGAAGGCAGt < 1:1158710/69‑1 (MQ=255) aCAGATATGTCCTAAAGTGTGACCCGGTGTGGCAATTACACTAAATTCATGCCCCAAAACGAAGGCAGt < 1:2188416/69‑1 (MQ=255) aCAGATATGTCCTAAAGTGTGACCCGGTGTGGCAATTACACTAAATTCATGCCCCAAAACGAAGGCAGt < 1:1140923/69‑1 (MQ=255) aCAGATATGTCCTAAAGTGTGACCCGGTGTGGCAATTACACTAAATTCATGCCCCAAAACGAAGGCAGt < 1:2361990/69‑1 (MQ=255) aCAGATATGTCCTAAAGTGTGACCCGGTGTGGCAATTACACTAAATTCATGCCCCAAAACGAAGGCAGt < 1:3289739/69‑1 (MQ=255) aCAGATATGTCCTAAAGTGTGACCCGGCGTAGCAATTACACTAAATTCATGCCCCaa > 1:351646/1‑57 (MQ=255) aCAGATATGTCCTAAAGTGTGACCCGGCGTAGCAATTACACTAAATTCATGCCCCaa > 1:3402804/1‑57 (MQ=255) aCAGATATGTCCTAAAGTGTGACCCGGCGTAGCAATTACACTAAATTCATGCCCCaa > 1:799483/1‑57 (MQ=255) aCAGATATGTCCTAAAGTGTGACCCGGCGTAGCAATTACACTAAATTCATGCCCCaa > 1:1844920/1‑57 (MQ=255) aCAGATATGTCCTAAAGTGTGACCCGGCGTAGCAATTACACTAAATTCATGCCCCaa > 1:1916734/1‑57 (MQ=255) aCAGATATGTCCTAAAGTGTGACCCGGCGTAGCAACTACACTAAATTCATGCCCCaa > 1:1476176/1‑57 (MQ=255) tCCTAAAGTGTGACCCGGCGTAGCAATTACACTAAATTCATGCCTCAAAACGAAGGCAGTTTCGCCATc > 1:1802469/1‑69 (MQ=255) tCCTAAAGTGTGACCCGGCGTAGCAATTACACTAAATTCATGCCCCAAAACGAAGGCAg < 1:1270241/59‑1 (MQ=255) tCCTAAAGTGTGACCCGGCGTAGCAATTACACTAAATTCATGCCCCAAAACGAAGGCAg < 1:948742/59‑1 (MQ=255) tCCTAAAGTGTGACCCGGCGTAGCAATTACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCCATc > 1:1660221/1‑69 (MQ=255) tCCTAAAGTGTGACCCGGCGTAGCAATTACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCCATc > 1:2862810/1‑69 (MQ=255) tCCTAAAGTGTGACCCGGCGTAGCAATTACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCCATc > 1:791902/1‑69 (MQ=255) tCCTAAAGTGTGACCCGGCGTAGCAATTACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCCATc > 1:701190/1‑69 (MQ=255) tCCTAAAGTGTGACCCGGCGTAGCAATTACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCCATc > 1:705095/1‑69 (MQ=255) aaaGTGTGACCCGGCGTAGCAATTACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCCATCttt < 1:1715914/68‑1 (MQ=255) aaaGTGTGACCCGGCGTAGCAATTACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCCATCttt < 1:925011/68‑1 (MQ=255) gtgtGACCCGGCGTAGCAATTACACTAAATTTATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACt < 1:561658/69‑1 (MQ=255) gtgtGACCCGGCGTAGCAATTACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACt < 1:3058530/69‑1 (MQ=255) aCCCGGCGTAGCAATTACACTAAATTCATGCCCCAAAACGAAGGCAg < 1:757882/47‑1 (MQ=255) ccGGTGTGGCAATTACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCTATCttt > 1:3120036/1‑58 (MQ=255) ccGGTGTGGCAATTACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCTATCTTTGACTACCtgtg > 1:1013461/1‑69 (MQ=255) ccGGTGTGGCAATTACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCTATCTTTGACTACCtgtg > 1:694486/1‑69 (MQ=255) ccGGTGTGGCAATTACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCTATCTTTGACTACCtgtg > 1:131182/1‑69 (MQ=255) ccGGTGTGGCAATTACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCTATCTTTGACTACCtgtg > 1:633509/1‑69 (MQ=255) ccGGTGTGGCAATTACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCTATCTTTGACTACCtgtg > 1:404950/1‑69 (MQ=255) ccGGTGTGGCAATTACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCTATCTTTGACTACCtgtg > 1:3007148/1‑69 (MQ=255) ccGGTGTGGCAATTACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCTATCTTTGACTACCtgtg > 1:2778198/1‑69 (MQ=255) ccGGTGTGGCAATTACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCTATCTTTGACTACCtgtg > 1:202543/1‑69 (MQ=255) ccGGTGTGGCAATTACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCTATCTTTGACTACCtgtg > 1:2502182/1‑69 (MQ=255) ccGGTGTGGCAATTACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCTATCTTTGACTACCtgt > 1:2296028/1‑68 (MQ=255) ccGGTGTGGCAATTACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCTATCTTTGACTACCtgt > 1:2441252/1‑68 (MQ=255) ccGGTGTGGCAATTACACTAAATTAATGCCCCAAAACGAAGGCAGTTTCGCTATCTTTGACTACCtgtg > 1:3123746/1‑69 (MQ=255) | GTCGCCGCAAAATAGATAAGGTTTACTGAAGTAACAGATATGTCCTAAAGTGTGACCCGGCGTAGCAATTACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTG > minE/213226‑213350 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |