Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,503,899 A→G 46.7% V274V (GTA→GTG uhpT → hexose phosphate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,503,8990AG46.7% 16.6 / 145.5 164V274V (GTA→GTGuhpThexose phosphate transporter
Reads supporting (aligned to +/- strand):  ref base A (28/59);  new base G (31/45);  total (59/105)
Fisher's exact test for biased strand distribution p-value = 3.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 164 total aligned reads displayed.

GAGTATGTGCTGAAAAACAAAGTGATCTGGCTGCTGTGCTTCGCCAACATTTTCCTCTATGTGGTACGTATTGGTATCGACCAGTGGTCAACCGTATACGCGTTCCAGGAACTGAAACTCTCTAAAGCGG  >  minE/2503834‑2503963
                                                                 |                                                                
gAGTATGTGCTGAAAAACAAAGTGATCTGGCTGCTGTGCTTCGCCAACATTTTCCTCTATGTGGTACg                                                                <  1:3025928/68‑1 (MQ=255)
gAGTATGTGCTGAAAAACAAAGTGATCTGGCTGCTGTGCTTCGCCAACATTTTCCTCTATGTGGTACg                                                                <  1:1816482/68‑1 (MQ=255)
 aGTATGTGCTGAAAAACAAAGTGATCTGGCTGCTGTGCTTCGCCAACATTTTCCTCTATGTGGTACg                                                                <  1:129399/67‑1 (MQ=255)
   tATGTGCTGAAAAACAAAGTGATCTGGCTGCTGTGCTTCGCCAACATTTTCCTCTATGTGGTACGTAtt                                                            >  1:1787686/1‑69 (MQ=255)
   tATGTGCTGAAAAACAAAGTGATCTGGCTGCTGTGCTTCGCCAACATTTTCCTCTATGTGGTACGTAtt                                                            >  1:1143977/1‑69 (MQ=255)
   tATGTGCTGAAAAACAAAGTGATCTGGCTGCTGTGCTTCGCCAACATTTTCCTCTATGTGGTACGTAtt                                                            >  1:346293/1‑69 (MQ=255)
   tATGTGCTGAAAAACAAAGTGATCTGGCTGCTGTGCTTCGCCAACATTTTCCTCTATGTGGTACGTAtt                                                            >  1:2486507/1‑69 (MQ=255)
     tgtgCTGAAAAACAAAGTGATCTGGCTGCTGTGCTTCGCCAACATTTTCCTCTATGTGGTACGTATTg                                                           >  1:2911317/1‑68 (MQ=255)
       tgCTGAAAAACAAAGTGATCTGGCTGCTGTGCTTCGCCAACATTTTCCTCTATGTGGTACGTATTGGt                                                         >  1:1752182/1‑68 (MQ=255)
       tgCTGAAAAACAAAGTGATCTGGCTGCTGTGCTTCGCCAACATTTTCCTCTATGTGGTACGTATTGGTa                                                        >  1:2698978/1‑69 (MQ=255)
       tgCTGAAAAACAAAGTGATCTGGCTGCTGTGCTTCGCCAACATTTTCCTCTATGTGGTACGTATTGGTa                                                        >  1:759466/1‑69 (MQ=255)
         cTGAAAAACAAAGTGATCTGGCTGCTGTGCTTTGCCAACATTTTCCTCTATGTGGTGCGTATTGGTATc                                                      <  1:1036109/69‑1 (MQ=255)
         cTGAAAAACAAAGTGATCTGGCTGCTGTGCTTTGCCAACATTTTCCTCTATGTGGTGCGTATTGGTATc                                                      <  1:2198987/69‑1 (MQ=255)
         cTGAAAAACAAAGTGATCTGGCTGCTGTGCTTTGCCAACATTTTCCTCTATGTGGTGCGTATTGGTATc                                                      <  1:2833673/69‑1 (MQ=255)
         cTGAAAAACAAAGTGATCTGGCTGCTGTGCTTTGCCAACATTTTCCTCTATGTGGTGCGTATTGGTATc                                                      <  1:1910069/69‑1 (MQ=255)
         cTGAAAAACAAAGTGATCTGGCTGCTGTGCTTTGCCAACATTTTCCTCTATGTGGTGCGTATTGGTATc                                                      <  1:242231/69‑1 (MQ=255)
         cTGAAAAACAAAGTGATCTGGCTGCTGTGCTTTGCCAACATTTTCCTCTATGTGGTGCGTATTGGTATc                                                      <  1:2222666/69‑1 (MQ=255)
         cTGAAAAACAAAGTGATCTGGCTGCTGTGCTTTGCCAACATTTTCCTCTATGTGGTGCGTATTGGTATc                                                      <  1:349807/69‑1 (MQ=255)
          tGAAAAACAAAGTGATCTGGCTGCTGTGCTTTGCCAACATTTTCCTCTATGTGGTGCGTATTGGTATc                                                      <  1:1309171/68‑1 (MQ=255)
          tGAAAAACAAAGTGATCTGGCTGCTGTGCTTTGCCAACATTTTCCTCTATGTGGTGCGTATTGGTATc                                                      <  1:762198/68‑1 (MQ=255)
            aaaaaCAAAGTGATCTGGCTGCTGTGCTTCGCCAACATTTTCCTCTATGTGGTACGTATTGGTATCg                                                     >  1:2835239/1‑67 (MQ=255)
            aaaaaCAAAGTGATCTGGCTGCTGTGCTTCGCCAACATTTTCCTCTATGTGGTACGTATTGGTATCg                                                     >  1:3402820/1‑67 (MQ=255)
              aaaCAAAGTGATCTGGCTGCTGTGCTTCGCCAACATTTTCCTCTATGTGGTACGTATTGGTATCGAcc                                                  <  1:1537279/68‑1 (MQ=255)
              aaaCAAAGTGATCTGGCTGCTGTGCTTCGCCAACATTTTCCTCTATGTGGTACGTATTGGTATCGAcc                                                  <  1:1696249/68‑1 (MQ=255)
              aaaCAAAGTGATCTGGCTGCTGTGCTTCGCCAACATTTTCCTCTATGTGGTACGTATTGGTATCGAcc                                                  <  1:840427/68‑1 (MQ=255)
              aaaCAAAGTGATCTGGCTGCTGTGCTTCGCCAACATTTTCCTCTATGTGGTACGTATTGGTATCGAcc                                                  <  1:1087109/68‑1 (MQ=255)
              aaaCAAAGTGATCTGGCTGCTGTGCTTCGCCAACATTTTCCTCTATGTGGTACGTATTGGTATCGAcc                                                  <  1:711904/68‑1 (MQ=255)
              aaaCAAAGTGATATGGCTGCTGTGCTTCGCCAACATTTTCCTCTATGTGGTACGTATTGGTATCGAcc                                                  <  1:3228895/68‑1 (MQ=255)
               aaCAAAGTGATCTGGCTGCTGTGCTTCGCCAACATTTTCCTCTATGTGGTACGTATTGGTATCGAcc                                                  <  1:1584511/67‑1 (MQ=255)
               aaCAAAGTGATCTGGCTGCTGTGCTTCGCCAACATTTTCCTCTATGTGGTACGTATTGGTATCGACCAg                                                <  1:745600/69‑1 (MQ=255)
               aaCAAAGTGATCTGGCTGCTGTGCTTCGCCAACATTTTCCTCTATGTGGTACGTATTGGTATCGACCAg                                                <  1:1242909/69‑1 (MQ=255)
               aaCAAAGTGATCTGGCTGCTGTGCTTCGCCAACATTTTCCTCTATGTGGTACGTATTGGTATCGACCAg                                                <  1:1371383/69‑1 (MQ=255)
               aaCAAAGTGATCTGGCTGCTGTGCTTCGCCAACATTTTCCTCTATGTGGTACGTATTGGTATCGACCAg                                                <  1:2316593/69‑1 (MQ=255)
               aaCAAAGTGATCTGGCTGCTGTGCTTCGCCAACATTTTCCTCTATGTGGTACGTATTGGTATCGACCAg                                                <  1:1665443/69‑1 (MQ=255)
               aaCAAAGTGATCTGGCTGCTGTGCTTCGCCAACATTTTCCTCTATGTGGTACGTATTGGTATCGACCAg                                                <  1:212970/69‑1 (MQ=255)
                aCAAAGTGATCTGGCTGCTGTGCTTCGCCAACATTTTCCTCTATGTGGTACGTATTGGTATCGACCAGt                                               >  1:645883/1‑69 (MQ=255)
                aCAAAGTGATCTGGCTGCTGTGCTTCGCCAACATTTTCCTCTATGTGGTACGTATTGGTATCGACCAGt                                               >  1:504303/1‑69 (MQ=255)
                aCAAAGTGATCTGGCTGCTGTGCTTCGCCAACATTTTCCTCTATGTGGTACGTATTGGTATCGACCAGt                                               >  1:446461/1‑69 (MQ=255)
                          cTGGCTGCTGTGCTTCGCCAACATTTTCCTCTATGTGGTACGTATTGGTATCGACCAGTGGTCAACCg                                      <  1:2003996/68‑1 (MQ=255)
                          cTGGCTGCTGTGCTTCGCCAACATTTTCCTCTATGTGGTACGTATTGGTATCGACCAGTGGTCAACCg                                      <  1:2453599/68‑1 (MQ=255)
                          cTGGCTGCTGTGCTTCGCCAACATTTTCCTCTATGTGGTACGTATTGGTATCGACCAGTGGTCAACCg                                      <  1:227717/68‑1 (MQ=255)
                          cTGGCTGCTGTGCTTCGCCAACATTTTCCTCTATGTGGTACGTATTGGTATCGACCAGTGGTCAACCg                                      <  1:1370222/68‑1 (MQ=255)
                          cTGGCTGCTGTGCTTCGCCAACATTTTCCTCTATGTGGTACGTATTGGTATCGACCAGTGGTCAACCg                                      <  1:1469622/68‑1 (MQ=255)
                          cTGGCTGCTGTGCTTCGCCAACATTTTCCTCTATGTGGTACGTATTGGTATCGACCAGTGGTCAACCg                                      <  1:2892891/68‑1 (MQ=255)
                          cTGGCTGCTGTGCTTCGCCAACATTTTCCTCTATGTGGTACGTATTGGTATCGACCAGTGGTCAACCg                                      <  1:1522726/68‑1 (MQ=255)
                          cTGGCTGCTGTGCTTCGCCAACATTTTCCTCTATGTGGTACGTATTGGTATCGACCAGTGGTCAACCg                                      <  1:3278975/68‑1 (MQ=255)
                               tgctgTGCTTCGCCAACATTTTCCTCTATGTGGTACGTATTGGTATCGACCAGTGGTCAACCGta                                    <  1:2732932/65‑1 (MQ=255)
                               tgctgTGCTTCGCCAACATTTTCCTCTATGTGGTACGTATTGGTATCGACCAGTGGTCAACCGta                                    <  1:2403322/65‑1 (MQ=255)
                               tgctgTGCTTCGCCAACATTTTCCTCTATGTGGTACGTATTGGTATCGACCAGTGGTCAACCGta                                    <  1:2532247/65‑1 (MQ=255)
                               tgctgTGCTTCGCCAACATTTTCCTCTATGTGGTACGTATTGGTATCGACCAGTGGTCAACCGta                                    <  1:2796443/65‑1 (MQ=255)
                               tgctgTGCTTCGCCAACATTTTCCTCTATGTGGTACGTATTGGTATCGACCAGTGGTCAACCGta                                    <  1:881950/65‑1 (MQ=255)
                               tgctgTGCTTCGCCAACATTTTCCTCTATGTGGTACGTATTGGTATCGACCAGTGGTCAACCGta                                    <  1:1591896/65‑1 (MQ=255)
                               tgctgTGCTTCGCCAACATTTTCCTCTATGTGGTACGTATTGGTATCGACCAGTGGTCAACCGta                                    <  1:1195461/65‑1 (MQ=255)
                                   gtgCTTCGCCAACATTTTCCTCTATGTGGTACGTATTGGTATCGACCAGTGGTCAACCGTATACGCGt                             >  1:1364683/1‑68 (MQ=255)
                                    tgCTTTGCCAACATTTTCCTCTATGTGGTGCGTATTGGTATCGCCCAGTGGTCAACCGTGTATGCGt                             >  1:1044114/1‑67 (MQ=255)
                                    tgCTTTGCCAACATTTTCCTCTATGTGGTGCGTATTGGTATCGACCAGTGGTCAACCGTGTAtgcg                              >  1:1732989/1‑66 (MQ=255)
                                    tgCTTTGCCAACATTTTCCTCTATGTGGTGCGTATTGGTATCGACCAGTGGTCAACCGTGTAtgcg                              >  1:497997/1‑66 (MQ=255)
                                    tgCTTTGCCAACATTTTCCTCTATGTGGTGCGTATTGGTATCGACCAGTGGTCAACCGTGTATGCGt                             >  1:2113201/1‑67 (MQ=255)
                                    tgCTTTGCCAACATTTTCCTCTATGTGGTGCGTATTGGTATCGACCAGTGGTCAACCGTGTATGCGt                             >  1:1026999/1‑67 (MQ=255)
                                    tgCTTTGCCAACATTTTCCTCTATGTGGTGCGTATTGGTATCGACCAGTGGTCAACCGTGTATGCGt                             >  1:1544483/1‑67 (MQ=255)
                                    tgCTTTGCCAACATTTTCCTCTATGTGGTGCGTATTGGTATCGACCAGTGGTCAACCGTGTATGCGt                             >  1:2209409/1‑67 (MQ=255)
                                    tgCTTTGCCAACATTTTCCTCTATGTGGTGCGTATTGGTATCGACCAGTGGTCAACCGTGTATGCGt                             >  1:2203091/1‑67 (MQ=255)
                                    tgCTTTGCCAACATTTTCCTCTATGTGGTGCGTATTGGTATCGACCAGTGGTCAACCGTGTATGCGt                             >  1:609577/1‑67 (MQ=255)
                                    tgCTTTGCCAACATTTTCCTCTATGTGGTGCGTATTGGTATCGACCAGTGGTCAACCGTGTATGCGt                             >  1:1630311/1‑67 (MQ=255)
                                    tgCTTTGCCAACATTTTCCTCTATGTGGTGCGTATTGGTATCGACCAGTGGTCAACCGTGTATGCGt                             >  1:1648676/1‑67 (MQ=255)
                                    tgCTTTGCCAACATTTTCCTCTATGTGGTGCGTATTGGTATCGACCAGTGGTCAACCGTGTATGCGt                             >  1:1150319/1‑67 (MQ=255)
                                    tgCTTTGCCAACATTTTCCTCTATGTGGTGCGTATTGGTATCGACCAGTGGTCAACCGTGTATGCGt                             >  1:168538/1‑67 (MQ=255)
                                    tgCTTTGCCAACATTTTCCTCTATGTGGTGCGTATTGGTATCGACCAGTGGTCAACCGTGTATGCGt                             >  1:1046220/1‑67 (MQ=255)
                                    tgCTTTGCCAACATTTTCCTCTATGTGGTGCGTATTGGTATCGACCAGTGGTCAACCGTGTATGCGt                             >  1:1895945/1‑67 (MQ=255)
                                    tgCTTTGCCAACATTTTCCTCTATGTGGTGCGTATTGGTATCGACCAGTGGTCAACCGTGTATGCGt                             >  1:1946329/1‑67 (MQ=255)
                                    tgCTTTGCCAACATTTTCCTCTATGTGGTGCGTATTGGTATCGACCAGTGGTCAACCGTGTATGCGt                             >  1:779386/1‑67 (MQ=255)
                                    tgCTTTGCCAACATTTTCCTCTATGTGGTGCGTATTGGTATCGACCAGTGGTCAACCGTGTATGCGt                             >  1:2545809/1‑67 (MQ=255)
                                    tgCTTTGCCAACATTTTCCTCTATGTGGTGCGTATTGGTATCGACCAGTGGTCAACCGTGTATGCGt                             >  1:2627681/1‑67 (MQ=255)
                                    agCTTTGCCAACATTATCCTCTATGTGGTGCGTATTGGTATCGACCAGAGGTCAACCGTGTATGCGt                             >  1:857912/2‑67 (MQ=255)
                                        tCGCCAACATTTTCCTCTATGTGGTACGTATTGGTATCGACCAGTGGTCAACCGTATACGCGTTCCAgg                       <  1:738383/69‑1 (MQ=255)
                                        tCGCCAACATTTTCCTCTATGTGGTACGTATTGGTATCGACCAGTGGTCAACCGTATACGCGTTCCAgg                       <  1:1200581/69‑1 (MQ=255)
                                        tCGCCAACATTTTCCTCTATGTGGTACGTATTGGTATCGACCAGTGGTCAACCGTATACGCGTTCCAgg                       <  1:1556361/69‑1 (MQ=255)
                                        tCGCCAACATTTTCCTCTATGTGGTACGTATTGGTATCGACCAGTGGTCAACCGTATACGCGTTCCAgg                       <  1:711298/69‑1 (MQ=255)
                                        tCGCCAACATTTTCCTCTATGTGGTACGTATTGGTATCGACCAGTGGTCAACCGTATACGCGTTCCAgg                       <  1:3067874/69‑1 (MQ=255)
                                          gCCAACATTTTCCTCTATGTGGTGCGTATTGGTATCGACCAGTGGTCaa                                         <  1:3207776/49‑1 (MQ=255)
                                          gCCAACATTTTCCTCTATGTGGTGCGTATTGGTATCGACCAGTGGTCaa                                         <  1:2272351/49‑1 (MQ=255)
                                          gCCAACATTTTCCTCTATGTGGTGCGTATTGGTATCGACCAGTGGTCaa                                         <  1:2067145/49‑1 (MQ=255)
                                          gCCAACATTTTCCTCTATGTGGTGCGTATTGGTATCGACCAGTGGTCaa                                         <  1:81409/49‑1 (MQ=255)
                                          gCCAACATTTTCCTCTATGTGGTGCGTATTGGTATCGACCAGTGGTCaa                                         <  1:786663/49‑1 (MQ=255)
                                          gCCAACATTTTCCTCTATGTGGTGCGTATTGGTATCGACCAGTGGTCaa                                         <  1:1957429/49‑1 (MQ=255)
                                          gCCAACATTTTCCTCTATGTGGTGCGTATTGGTATCGACCAGTGGTCaa                                         <  1:1888072/49‑1 (MQ=255)
                                          gCCAACATTTTCCTCTATGTGGTGCGTATTGGTATCGACCAGTGGTCaa                                         <  1:3173352/49‑1 (MQ=255)
                                          gCCAACATTTTCCTCTATGTGGTGCGTATTGGTATCGACCAGTGGTCaa                                         <  1:3193535/49‑1 (MQ=255)
                                          gCCAACATTTTCCTCTATGTGGTGCGTATTGGTATCGACCAGTGGTCaa                                         <  1:410972/49‑1 (MQ=255)
                                          gCCAACATTTTCCTCTATGTGGTGCGTATTGGTATCGACCAGTGGTCaa                                         <  1:3327314/49‑1 (MQ=255)
                                          gCCAACATTTTCCTCTATGTGGTGCGTATTGGTATCGACCAGTGGTCaa                                         <  1:671966/49‑1 (MQ=255)
                                          gCCAACATTTTCCTCTATGTGGTGCGTATTGGTATCGACCAGTGGTCaa                                         <  1:659306/49‑1 (MQ=255)
                                          gCCAACATTTTCCTCTATGTGGTGCGTATTGGTATCGACCAGTGGTCaa                                         <  1:332910/49‑1 (MQ=255)
                                          gCCAACATTTTCCTCTATGTGGTGCGTATTGGTATCGACCAGTGGTCaa                                         <  1:557963/49‑1 (MQ=255)
                                          gCCAACATTTTCCTCTATGTGGTGCGTATTGGTATCGACCAGTGGTCaa                                         <  1:530149/49‑1 (MQ=255)
                                          gCCAACATTTTCCTCTATGTGGTGCGTATTGGTATCGACCAGTGGTCaa                                         <  1:2341136/49‑1 (MQ=255)
                                           ccAACATTTTCCTCTATGTGGTGCGTATTGGTATCGACCAGTGGTCaa                                         <  1:1217866/48‑1 (MQ=255)
                                           ccAACATTTTCCTCTATGTGGTGCGTATTGGTATCGACCAGTGGTCaa                                         <  1:410140/48‑1 (MQ=255)
                                           ccAACATTTTCCTCTATGTGGTGCGTATTGGTATCGACCAGTGGTCaa                                         <  1:2180505/48‑1 (MQ=255)
                                                             tgGTACGTATTGGTATCGACCAGTGGTCAACCGTATACGCGTTCCAGGAACTGAAACTCTCTAAAGCgg  >  1:1393887/1‑69 (MQ=255)
                                                                 |                                                                
GAGTATGTGCTGAAAAACAAAGTGATCTGGCTGCTGTGCTTCGCCAACATTTTCCTCTATGTGGTACGTATTGGTATCGACCAGTGGTCAACCGTATACGCGTTCCAGGAACTGAAACTCTCTAAAGCGG  >  minE/2503834‑2503963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: