| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,503,968 | G→A | 50.0% | A297A (GCG→GCA) | uhpT → | hexose phosphate transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,503,968 | 0 | G | A | 50.0% | ‑5.2 / 39.8 | 46 | A297A (GCG→GCA) | uhpT | hexose phosphate transporter |
| Reads supporting (aligned to +/- strand): ref base G (15/8); new base A (8/15); total (23/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.58e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01 | |||||||||||
TATTGGTATCGACCAGTGGTCAACCGTATACGCGTTCCAGGAACTGAAACTCTCTAAAGCGGTGGCGATTCAGGGCTTTACGCTGTTTGAAGCTGGTGCGCTGGTCGGTACGCTGCTGTGGGGCTGG > minE/2503902‑2504028 | tATTGGTATCGACCAGTGGTCAACCGTATACGCGTTCCAGGAACTGAAACTCTCTAAAGCGGTGGCGa > 1:3307075/1‑68 (MQ=255)tATTGGTATCGACCAGTGGTCAACCGTATACGCGTTCCAGGAACTGAAACTCTCTAAAGCGGTGGCGa > 1:743231/1‑68 (MQ=255) gTCAACCGTATACGCGTTCCAGGAACTGAAACTCTCTAAAGCGGTGGCGATTCAGGGCTTTACGCTGt > 1:1586840/1‑68 (MQ=255) tataCGCGTTCCAGGAACTGAAACTCTCTAAAGCGGTGGCGATTCAGGGCTTTACGCTGTTTGAAGCTg > 1:1844258/1‑69 (MQ=255) tataCGCGTTCCAGGAACTGAAACTCTCTAAAGCGGTGGCGATTCAGGGCTTTACGCTGTTTGAAGCTg > 1:1772548/1‑69 (MQ=255) tataCGCGTTCCAGGAACTGAAACTCTCTAAAGCGGTGGCGATTCAGGGCTTTACGCTGTTTGAAGCTg > 1:1440955/1‑69 (MQ=255) tataCGCGTTCCAGGAACTGAAACTCTCTAAAGCGGTGGCGATTCAGGGCTTTACGCTGTTTGAAGCTg > 1:3006703/1‑69 (MQ=255) cgTTCCATGAACTGAAACTCTCTAAAGCGGTGGCAATTCAGGGCTTTACGCTGTTTGAAGCTGGTgcct < 1:676161/69‑3 (MQ=255) cgTTCCAGGAACTGAAACTCTCTAAAGCGGTGGCAATTCAGGGCTTTACGCTGTTTg > 1:2880828/1‑57 (MQ=255) cgTTCCAGGAACTGAAACTCTCTAAAGCGGTGGCAATTCAGGGCTTTACGCTGTTTg > 1:2082610/1‑57 (MQ=255) cgTTCCAGGAACTGAAACTCTCTAAAGCGGTGGCAATTCAGGGCTTTACGCTGTTTg > 1:1981454/1‑57 (MQ=255) cgTTCCAGGAACTGAAACTCTCTAAAGCGGTGGCAATTCAGGGCTTTACGCTGTTTg > 1:2114959/1‑57 (MQ=255) cgTTCCAGGAACTGAAACTCTCTAAAGCGGTGGCAATTCAGGGCTTTACGCTGTTTg > 1:1313435/1‑57 (MQ=255) cgTTCCAGGAACTGAAACTCTCTAAAGCGGTGGCAATTCAGGGCTTTACGCTGTTTg > 1:2748845/1‑57 (MQ=255) cgTTCCAGGAACTGAAACTCTCTAAAGCGGTGGCAATTCAGGGCTTTACGCTGTTTg > 1:2984967/1‑57 (MQ=255) cgTTCCAGGAACTGAAACTCTCTAAAGCGGTGGCAATTCAGGGCTTTACGCTGTTTg > 1:2831344/1‑57 (MQ=255) ttCCAGGAACTGAAACTCTCTAAAGCGGTGGCAATTCAGGGCTTTACGCTGTTTGAAGCTGGTGCGCt < 1:321012/68‑1 (MQ=255) ttCCAGGAACTGAAACTCTCTAAAGCGGTGGCAATTCAGGGCTTTACGCTGTTTGAAGCTGGTGCGCt < 1:2132022/68‑1 (MQ=255) ttCCAGGAACTGAAACTCTCTAAAGCGGTGGCAATTCAGGGCTTTACGCTGTTTGAAGCTGGTGCGCt < 1:3006365/68‑1 (MQ=255) ttCCAGGAACTGAAACTCTCTAAAGCGGTGGCAATTCAGGGCTTTACGCTGTTTGAAGCTGGTGCGCt < 1:1677914/68‑1 (MQ=255) ttCCAGGAACTGAAACTCTCTAAAGCGGTGGCAATTCAGGGCTTTACGCTGTTTGAAGCTGGTGCGCt < 1:449862/68‑1 (MQ=255) ttCCAGGAACTGAAACTCTCTAAAGCGGTGGCAATTCAGGGCTTTACGCTGTTTGAAGCTGGTGCGCt < 1:486870/68‑1 (MQ=255) ttCCAGGAACTGAAACTCTCTAAAGCGGTGGCAATTCAGGGCTTTACGCTGTTTGAAGCTGGTGCGCt < 1:1548833/68‑1 (MQ=255) ttCCAGGAACTGAAACTCTCTAAAGCGGTGGCAATTCAGGGCTTTACGCTGTTTGAAGCTGGTGCGCt < 1:1523492/68‑1 (MQ=255) ttCCAGGAACTGAAACTCTCTAAAGCGGTGGCAATTCAGGGCTTTACGCTGTTTGAAGCTGGTGCGCt < 1:1504946/68‑1 (MQ=255) ttCCAGGAACTGAAACTCTCTAAAGCGGTGGCAATTCAGGGCTTTACGCTGTTTGAAGCTGGTGCGCt < 1:1298032/68‑1 (MQ=255) ttCCAGGAACTGAAACTCTCTAAAGCGGTGGCAATTCAGGGCTTTACGCTGTTTGAAGCTGGTGCGCt < 1:858789/68‑1 (MQ=255) ttCCAGGAACTGAAACTCTCTAAAGCGGTGGCAATTCAGGGCTTTACGCTGTTTGAAGCTGGTGCGCt < 1:1247485/68‑1 (MQ=255) ttCCAGGAACTGAAACTCTCTAAAGCGGTGGCAATTCAGGGCTTTACGCTGTTTGAAGCTGGTGCGCt < 1:1184025/68‑1 (MQ=255) ttCCAGGAACTGAAACTCTCTAAAGCGGTGGCAATTCAGGGCTTTACGCTGTTTGAAGCTGGTGCGCt < 1:999546/68‑1 (MQ=255) ggAACTGAAACTCTCTAAAGCTGTGGCGATTCAGGGCTTTAGGCTGTTTTAAGCTTGTGCGCTGGTCgg < 1:212842/69‑1 (MQ=255) ggAACTGAAACTCTCTAAAGCGGTGGCGATTCAGGGCTTTACGCTGTTTGAAGCTTGTGCGCTGGTCgg < 1:164238/69‑1 (MQ=255) ggAACTGAAACTCTCTAAAGCGGTGGCGATTCAGGGCTTTACGCTGTTTGAAGCTGGTGCGCTGGTCgg < 1:2605802/69‑1 (MQ=255) ggAACTGAAACTCTCTAAAGCGGTGGCGATTCAGGGCTTTACGCTGTTTGAAGCTGGTGCGCTGGTCgg < 1:1078012/69‑1 (MQ=255) ggAACTGAAACTCTCTAAAGCGGTGGCGATTCAGGGCTTTACGCTGTTTGAAGCTGGTGCGCTGGTCgg < 1:206481/69‑1 (MQ=255) ggAACTGAAACTCTCTAAAGCGGTGGCGATTCAGGGCTTTACGCTGTTTGAAGCTGGTGCGCTGGTCgg < 1:1478837/69‑1 (MQ=255) ggAACTGAAACTCTCTAAAGCGGTGGCGATTCAGGGCTTTACGCTGTTTGAAGCTGGTGCGCTGGTCgg < 1:763173/69‑1 (MQ=255) gAAACTCTCTAAAGCGGTGGCGATTCAGGGCTTTACGCTGTTTGAGGCTGGTGCGCTGGTCGGTACgct > 1:2116453/1‑69 (MQ=255) tAAAGCGGTGGCGATTCAGGGCTTTACGCTGTTTGAAGCTGGTGCGCTGGTCGGTACGCTGCTGTgggg > 1:1144378/1‑69 (MQ=255) aaGCGGTGGCGATTCAGGGCTTTACGCTGTTTGAAGCTGGTGCGCTGGTCGGTACGCTGCTGTGGggct > 1:2755911/1‑69 (MQ=255) aaGCGGTGGCGATTCAGGGCTTTACGCTGTTTGAAGCTGGTGCGCTGGTCGGTACGCTGCTGTGGggct > 1:1282726/1‑69 (MQ=255) aaGCGGTGGCGATTCAGGGCTTTACGCTGTTTGAAGCTGGTGCGCTGGTCGGTACGCTGCTGTGGggct > 1:951137/1‑69 (MQ=255) aGCGGTGGCGATTCAGGGCTTTACGCTGTTTGAAGCTGGTGCGCTGGTCGGTACGCTGCTGTGGggctg > 1:2773806/1‑69 (MQ=255) aGCGGTGGCGATTCAGGGCTTTACGCTGTTTGAAGCTGGTGCGCTGGTCGGTACGCTGCTGTGGggctg > 1:340935/1‑69 (MQ=255) aGCGGTGGCGATTCAGGGCTTTACGCTGTTTGAAGCTGGTGCGCTGGTCGGTACGCTGCTGTGGggctg > 1:916339/1‑69 (MQ=255) gCGGTGGCGATTCAGGGCTTTACGCTGTTTGAAGCTGGTGCGCTGGTCGGTACGCTGCTGTGGggctgg < 1:1638773/69‑1 (MQ=255) | TATTGGTATCGACCAGTGGTCAACCGTATACGCGTTCCAGGAACTGAAACTCTCTAAAGCGGTGGCGATTCAGGGCTTTACGCTGTTTGAAGCTGGTGCGCTGGTCGGTACGCTGCTGTGGGGCTGG > minE/2503902‑2504028 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |