Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,505,679 T→A 54.5% G45G (GGT→GGA)  yicJ → predicted transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,505,6790TA54.5% 27.3 / 109.9 101G45G (GGT→GGA) yicJpredicted transporter
Reads supporting (aligned to +/- strand):  ref base T (11/35);  new base A (7/48);  total (18/83)
Fisher's exact test for biased strand distribution p-value = 1.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 101 total aligned reads displayed.

TAATGTTATATATGATGTTCTTTTATACCGATATTTTTGGCATTCCTGCCGGTTTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATGCGATTTCCGATCCTTGCATGGGG  >  minE/2505627‑2505742
                                                    |                                                               
tAATGTTATATTTGATGTTCTTTTATACCGATATTTTTGGCATTCCTGCCGGATTTGTCGGAACCATGt                                                 >  1:3020857/1‑69 (MQ=255)
tAATGTTATATATGATGTTCTTTTATACCGATATTTTTGGCATTCCTGCCGGATTTGTCGGAACCATGt                                                 >  1:3342587/1‑69 (MQ=255)
tAATGTTATATATGATGTTCTTTTATACCGATATTTTTGGCATTCCTGCCGGATTTGTCGGAACCATGt                                                 >  1:698797/1‑69 (MQ=255)
tAATGTTATATATGATGTTCTTTTATACCGATATTTTTGGCATTCCTGCCGGATTTGTCGGAACCATGt                                                 >  1:1907274/1‑69 (MQ=255)
tAATGTTATATATGATGTTCTTTTATACCGATATTTTTGGCATTCCTGCCGGATTTGTCGGAACCATGt                                                 >  1:1909737/1‑69 (MQ=255)
tAATGTTATATATGATGTTCTTTTATACCGATATTTTTGGCATTCCTGCCGGATTTGTCGGAACCATGt                                                 >  1:47631/1‑69 (MQ=255)
tAATGTTATATATGATGTTCTTTTATACCGATATTTTTGGCATTCCTGCCGGATTTGTCGGAACCATGt                                                 >  1:309320/1‑69 (MQ=255)
  aTGTTATATATGATGTTCTTTTATACCGATATTTTTGGCATTCCTGCCGGTTTTGTCGGAACCATGttt                                               >  1:692494/1‑69 (MQ=255)
                gTTCTTTTATACCGATATTTTTGGCATTCCTGCCGGATTTGTCGGAACCATGTTTTTGGTCGCTCGTg                                  <  1:3387140/68‑1 (MQ=255)
                gTTCTTTTATACCGATATTTTTGGCATTCCTGCCGGATTTGTCGGAACCATGTTTTTGGTCGCTCGTg                                  <  1:332558/68‑1 (MQ=255)
                gTTCTTTTATACCGATATTTTTGGCATTCCTGCCGGATTTGTCGGAACCATGTTTTTGGTCGCTCGTg                                  <  1:2058532/68‑1 (MQ=255)
                gTTCTTTTATACCGATATTTTTGGCATTCCTGCCGGATTTGTCGGAACCATGTTTTTGGTCGCTCGTg                                  <  1:2020833/68‑1 (MQ=255)
                gTTCTTTTATACCGATATTTTTGGCATTCCTGCCGGATTTGTCGGAACCATGTTTTTGGTCGCTCGTg                                  <  1:2194310/68‑1 (MQ=255)
                gTTCTTTTATACCGATATTTTTGGCATTCCTGCCGGATTTGTCGGAACCATGTTTTTGGTCGCTCGTg                                  <  1:3386284/68‑1 (MQ=255)
                gTTCTTTTATACCGATATTTTTGGCATTCCTGCCGGATTTGTCGGAACCATGTTTTTGGTCGCTCGTg                                  <  1:186835/68‑1 (MQ=255)
                gTTCTTTTATACCGATATTTTTGGCATTCCTGCCGGATTTGTCGGAACCATGTTTTTGGTCGCTCGTg                                  <  1:1825500/68‑1 (MQ=255)
                gTTCTTTTATACCGATATTTTTGGCATTCCTGCCGGATTTGTCGGAACCATGTTTTTGGTCGCTCGTg                                  <  1:1003991/68‑1 (MQ=255)
                gTTCTTTTATACCGATATTTTTGGCATTCCTGCCGGATTTGTCGGAACCATGTTTTTGGTCGCTCGTg                                  <  1:65126/68‑1 (MQ=255)
                gTTCTTTTATACCGATATTTTTGGCATTCCTGCCGGATTTGTCGGAACCATGTTTTTGGTCGCTCGTg                                  <  1:1037839/68‑1 (MQ=255)
                gTTCTTTTATACCGATATTTTTGGCATTCCTGCCGGATTTGTCGGAACCATGTTTTTGGTCGCTCGTg                                  <  1:736252/68‑1 (MQ=255)
                gTTCTTTTATACCGATATTTTTGGCATTCCTGCCGGATTTGTCGGAACCATGTTTTTGGTCGCTCGTg                                  <  1:113259/68‑1 (MQ=255)
                gTTCTTTTATACCGATATTTTTGGCATTCCTGCCGGATTTGTCGGAACCATGTTTTTGGTCGCTCGTg                                  <  1:1394927/68‑1 (MQ=255)
                gTTCTTTTATACCGATATTTTTGGCATTCCTGCCGGATTTGTCGGAACCATGTTTTTGGTCGCTCGTg                                  <  1:3126326/68‑1 (MQ=255)
                gTTCTTTTATACCGATATTTTTGGCATTCCTGCCGGATTTGTCGGAACCATGTTTTTGGTCGCTCGTg                                  <  1:1151700/68‑1 (MQ=255)
                gTTCTTTTATACCGATATTTTTGGCATTCCTGCCGGATTTGTCGGAACCATGTTTTTGGTCGCTCGTg                                  <  1:863498/68‑1 (MQ=255)
                gTTCTTTTATACCGATATTTTTGGCATTCCTGCCGGATTTGTCGGAACCATGTTTTTGGTCGCTCGTg                                  <  1:1257422/68‑1 (MQ=255)
                gTTCTTTTATACCGATATTTTTGGCATTCCTGCCGGATTTGTCGGAACCATGTTTTTGGTCGCTCGTg                                  <  1:779253/68‑1 (MQ=255)
                gTTCTTTTATACCGATATTTTTGGCATTCCTGCCGGATTTGTCGGAACCATGTTTTTGGTCGCTCGTg                                  <  1:1497712/68‑1 (MQ=255)
                 ttCTTTTATACCGATATTTTTGGCATTCCTGCCGGTTTTGTCGGAACCATGTTTTTGGTCGCt                                      <  1:2855030/63‑1 (MQ=255)
                 ttCTTTTATACCGATATTTTTGGCATTCCTGCCGGTTTTGTCGGAACCATGTTTTTGGTCGCt                                      <  1:3159317/63‑1 (MQ=255)
                   cTTTTATACCGATATTTTTGGCATTCCTGCCGGTTTTGTCGGAACCATGTTTTTGGTCGCTCGTGCAc                               >  1:2454951/1‑68 (MQ=255)
                        ataCCGATATTTTTGGCATTCCTGCCGGATTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTgg                            <  1:3243301/66‑1 (MQ=255)
                        ataCCGATATTTTTGGCATTCCTGCCGGATTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTgg                            <  1:2838895/66‑1 (MQ=255)
                        ataCCGATATTTTTGGCATTCCTGCCGGATTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTgg                            <  1:1243196/66‑1 (MQ=255)
                        ataCCGATATTTTTGGCATTCCTGCCGGATTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTgg                            <  1:2032716/66‑1 (MQ=255)
                        ataCCGATATTTTTGGCATTCCTGCCGGATTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTgg                            <  1:763654/66‑1 (MQ=255)
                        ataCCGATATTTTTGGCATTCCTGCCGGATTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTgg                            <  1:3210699/66‑1 (MQ=255)
                        ataCCGATATTTTTGGCATTCCTGCCGGATTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTgg                            <  1:2084343/66‑1 (MQ=255)
                        ataCCGATATTTTTGGCATTCCTGCCGGATTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTgg                            <  1:2185030/66‑1 (MQ=255)
                        ataCCGATATTTTTGGCATTCCTGCCGGATTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTgg                            <  1:1415184/66‑1 (MQ=255)
                        ataCCGATATTTTTGGCATTCCTGCCGGATTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTgg                            <  1:2322986/66‑1 (MQ=255)
                        ataCCGATATTTTTGGCATTCCTGCCGGATTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTgg                            <  1:1076256/66‑1 (MQ=255)
                          aCCGATATTTTTGGCATTCCTGCCGGTTTTGTCGGAACCCTGTTTTTGGTCGCTCGTGCACTGGATGc                        <  1:2457644/68‑1 (MQ=255)
                          aCCGATATTTTTGGCATTCCTGCCGGTTTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATGc                        <  1:1955108/68‑1 (MQ=255)
                          aCCGATATTTTTGGCATTCCTGCCGGTTTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATGc                        <  1:804356/68‑1 (MQ=255)
                          aCCGATATTTTTGGCATTCCTGCCCGTTTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATGc                        <  1:636311/68‑1 (MQ=255)
                           ccGATATTTTTGGCATTCCTGCCGGTTTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATga                        <  1:1754540/67‑2 (MQ=255)
                                         aTTCCTGCCGGTTTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATGCGATTTCCGATCCTTg         <  1:3383297/68‑1 (MQ=255)
                                         aTTCCTGCCGGTTTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATGCGATTTCCGATCCTTg         <  1:433952/68‑1 (MQ=255)
                                         aTTCCTGCCGGTTTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATGCGATTTCCGATCCTTg         <  1:2661203/68‑1 (MQ=255)
                                         aTTCCTGCCGGTTTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATGCGATTTCCGATCCTTg         <  1:3242433/68‑1 (MQ=255)
                                         aTTCCTGCCGGTTTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATGCGATTTCCGATCCTTg         <  1:3210180/68‑1 (MQ=255)
                                         aTTCCTGCCGGTTTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATGCGATTTCCGATCCTTg         <  1:753189/68‑1 (MQ=255)
                                         aTTCCTGCCGGTTTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATGCGATTTCCGATCCTTg         <  1:851199/68‑1 (MQ=255)
                                         aTTCCTGCCGGTTTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATGCGATTTCCGATCCTTg         <  1:870455/68‑1 (MQ=255)
                                         aTTCCTGCCGGTTTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATGCGATTTCCGATCCTTg         <  1:918557/68‑1 (MQ=255)
                                         aTTCCTGCCGGTTTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATGCGATTTCCGATCCTTg         <  1:937305/68‑1 (MQ=255)
                                         aTTCCTGCCGGTTTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATGCGATTTCCGATCCTTg         <  1:3081022/68‑1 (MQ=255)
                                         aTTCCTGCCGGTTTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATGCGATTTCCGATCCTTg         <  1:134841/68‑1 (MQ=255)
                                         aTTCCTGCCGGTTTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATGCGATTTCCGATCCTTg         <  1:3050778/68‑1 (MQ=255)
                                         aTTCCTGCCGGTTTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATGCGATTTCCGATCCTTg         <  1:2935248/68‑1 (MQ=255)
                                         aTTCCTGCCGGTTTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATGCGATTTCCGATCCTTg         <  1:1245904/68‑1 (MQ=255)
                                         aTTCCTGCCGGTTTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATGCGATTTCCGATCCTTg         <  1:2101594/68‑1 (MQ=255)
                                         aTTCCTGCCGGTTTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATGCGATTTCCGATCCTTg         <  1:2560023/68‑1 (MQ=255)
                                         aTTCCTGCCGGTTTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATGCGATTTCCGATCCTTg         <  1:1044051/68‑1 (MQ=255)
                                         aTTCCTGCCGGTTTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATGCGATTTCCGATCCTTg         <  1:1159377/68‑1 (MQ=255)
                                            ccTTCCGGTTTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATGCGATTTCCGa               <  1:1189762/59‑1 (MQ=255)
                                            ccTGCCGGTTTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATGCGATTTCCGa               <  1:3058843/59‑1 (MQ=255)
                                            ccTGCCGGTTTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATGCGATTTCCGa               <  1:542448/59‑1 (MQ=255)
                                            ccTGCCGGTTTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATGCGATTTCCGa               <  1:2347056/59‑1 (MQ=255)
                                            ccTGCCGGTTTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATGCGATTTCCGa               <  1:2504953/59‑1 (MQ=255)
                                            ccTGCCGGTTTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATGCGATTTCCGa               <  1:3215113/59‑1 (MQ=255)
                                            ccTGCCGGTTTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATGCGATTTCCGa               <  1:1042253/59‑1 (MQ=255)
                                            ccTGCCGGTTTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATGCGATTTCCGa               <  1:3052173/59‑1 (MQ=255)
                                            ccTGCCGGTTTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATGCGATTTCCGATCCTTGCAt      >  1:239268/1‑68 (MQ=255)
                                            ccTGCCGGTTTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATGCGATTTCCGATCCTTGCATg     >  1:1217240/1‑69 (MQ=255)
                                            ccTGCCGGTTTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATGCGATTTCCGATCCTTGCATg     >  1:2631830/1‑69 (MQ=255)
                                            ccTGCCGGTTTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATGCGATTTCCGATCCTTGCATg     >  1:2069856/1‑69 (MQ=255)
                                            ccTGCCGGTTTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATGCGATTTCCGATCCTTGCATg     >  1:466557/1‑69 (MQ=255)
                                            ccTGCCGGTTTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATGCGATTTCCGATCCTTGCATg     >  1:1324785/1‑69 (MQ=255)
                                            ccTGCCGGTTTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATGCGATTTCCGATCCTTGCATg     >  1:153865/1‑69 (MQ=255)
                                            ccTGCCGGTTTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATGCGATTTCCGATCCTTGCATg     >  1:1628266/1‑69 (MQ=255)
                                            ccTGCCGGTTTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATGCGATTTCCGATCCTTGCATg     >  1:1571716/1‑69 (MQ=255)
                                               gCCGGATTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATGCGATTTCCGATCCTTGCATgggg  <  1:1145119/69‑1 (MQ=255)
                                               gCCGGATTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATGCGATTTCCGATCCTTGCATgggg  <  1:949230/69‑1 (MQ=255)
                                               gCCGGATTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATGCGATTTCCGATCCTTGCATgggg  <  1:965921/69‑1 (MQ=255)
                                               gCCGGATTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATGCGATTTCCGATCCTTGCATgggg  <  1:1481525/69‑1 (MQ=255)
                                               gCCGGATTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATGCGATTTCCGATCCTTGCATgggg  <  1:1822441/69‑1 (MQ=255)
                                               gCCGGATTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATGCGATTTCCGATCCTTGCATgggg  <  1:612460/69‑1 (MQ=255)
                                               gCCGGATTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATGCGATTTCCGATCCTTGCATgggg  <  1:601308/69‑1 (MQ=255)
                                               gCCGGATTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATGCGATTTCCGATCCTTGCATgggg  <  1:596046/69‑1 (MQ=255)
                                               gCCGGATTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATGCGATTTCCGATCCTTGCATgggg  <  1:2014364/69‑1 (MQ=255)
                                               gCCGGATTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATGCGATTTCCGATCCTTGCATgggg  <  1:2383183/69‑1 (MQ=255)
                                               gCCGGATTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATGCGATTTCCGATCCTTGCATgggg  <  1:3309340/69‑1 (MQ=255)
                                               gCCGGATTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATGCGATTTCCGATCCTTGCATgggg  <  1:3298347/69‑1 (MQ=255)
                                               gCCGGATTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATGCGATTTCCGATCCTTGCATgggg  <  1:2524561/69‑1 (MQ=255)
                                               gCCGGATTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATGCGATTTCCGATCCTTGCATgggg  <  1:2558849/69‑1 (MQ=255)
                                               gCCGGATTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATGCGATTTCCGATCCTTGCATgggg  <  1:2830590/69‑1 (MQ=255)
                                               gCCGGATTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATGCGATTTCCGATCCTTGCATgggg  <  1:2906517/69‑1 (MQ=255)
                                               gCCGGATTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATGCGATTTCCGATCCTTGCATgggg  <  1:3108866/69‑1 (MQ=255)
                                                    |                                                               
TAATGTTATATATGATGTTCTTTTATACCGATATTTTTGGCATTCCTGCCGGTTTTGTCGGAACCATGTTTTTGGTCGCTCGTGCACTGGATGCGATTTCCGATCCTTGCATGGGG  >  minE/2505627‑2505742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑