BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,505,874	C→T	11.2%	I110I (ATC→ATT)	yicJ →	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,505,874	0	C	T	11.2%	189.0 / 8.1	96	I110I (ATC→ATT)	yicJ	predicted transporter
Reads supporting (aligned to +/- strand): ref base C (53/32); new base T (3/8); total (56/40)
Fisher's exact test for biased strand distribution p-value = 4.74e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 105 total aligned reads displayed.

CTGCCATTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACCGTCGTCAATATCCCTTACTGCG  >  minE/2505806‑2505938

cTGCCATTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCGGATCTCAGTATGAACGGCAAAATGATc                                                                  <  1:608431/69‑1 (MQ=255)

cTGCCATTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATc                                                                  <  1:3181921/69‑1 (MQ=255)

cTGCCATTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATc                                                                  <  1:2030443/69‑1 (MQ=255)

cTGCCATTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATc                                                                  >  1:3354679/1‑69 (MQ=255)

cTGCCATTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATc                                                                  <  1:2446071/69‑1 (MQ=255)

cTGCCATTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATc                                                                  <  1:6494/69‑1 (MQ=255)

cTGCCATTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATc                                                                  <  1:1758167/69‑1 (MQ=255)

cTGCCATTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATc                                                                  <  1:2521193/69‑1 (MQ=255)

 tGCCATTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATCt                                                                 <  1:2792443/69‑1 (MQ=255)

  gCCATTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTa                                                                >  1:1000073/1‑69 (MQ=255)

  gCCATTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTa                                                                >  1:1573311/1‑69 (MQ=255)

  gCCATTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTa                                                                >  1:3398244/1‑69 (MQ=255)

  gCCATTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTa                                                                >  1:1428270/1‑69 (MQ=255)

  gCCATTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTa                                                                >  1:451319/1‑69 (MQ=255)

  gCCATTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTa                                                                >  1:1864074/1‑69 (MQ=255)

  gCCATTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTa                                                                >  1:2292324/1‑69 (MQ=255)

  gCCATTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTa                                                                >  1:1046318/1‑69 (MQ=255)

        cGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATgcagc                                                          >  1:2379145/1‑69 (MQ=255)

        cGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATgcagc                                                          >  1:2819015/1‑69 (MQ=255)

        cGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATgcagc                                                          >  1:1983094/1‑69 (MQ=255)

        cGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATgcagc                                                          >  1:2946534/1‑69 (MQ=255)

        cGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATgcagc                                                          >  1:2153562/1‑69 (MQ=255)

        cGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATgcagc                                                          >  1:1690934/1‑69 (MQ=255)

        cGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATgcagc                                                          >  1:1689628/1‑69 (MQ=255)

        cGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATgcagc                                                          >  1:435313/1‑69 (MQ=255)

        cGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATgcagc                                                          >  1:1685434/1‑69 (MQ=255)

        cGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATgcagc                                                          >  1:703103/1‑69 (MQ=255)

           gATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGATTTATGCAGCAAt                                                       >  1:1484798/1‑69 (MQ=255)

           gATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGATTTATGCAGCAAt                                                       >  1:1367291/1‑69 (MQ=255)

           gATCGTCTGTGTACTGGCCTATAGCACGCCAGATCCCAGTATGAACGGAAAAATGATTTATGCAGCAAt                                                       >  1:2447248/1‑69 (MQ=255)

                 cTGTTTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCAAttactta                                                 <  1:1137668/69‑1 (MQ=255)

                 cTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCAAttactta                                                 <  1:3049881/69‑1 (MQ=255)

                 cTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCAAttactta                                                 <  1:2135895/69‑1 (MQ=255)

                 cTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCAAttactta                                                 <  1:1410994/69‑1 (MQ=255)

                 cTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCAAttactta                                                 <  1:2274384/69‑1 (MQ=255)

                 cTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCAAttactta                                                 <  1:2279137/69‑1 (MQ=255)

                 cTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCAAttactta                                                 <  1:715524/69‑1 (MQ=255)

                 cTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCAAttactta                                                 <  1:700442/69‑1 (MQ=255)

                  tgtgTACTGGCCTATATCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCAAttactta                                                 <  1:2913430/68‑1 (MQ=255)

                        cTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTa                                          >  1:685335/1‑69 (MQ=255)

                        cTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTa                                          >  1:2777242/1‑69 (MQ=255)

                                taGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTActtacctt                                  >  1:1801813/1‑69 (MQ=255)

                                  gCACGCCCGATCTCAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTActtacctta                                 <  1:3250288/68‑1 (MQ=255)

                                  gCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTActtacctta                                 <  1:2192390/68‑1 (MQ=255)

                                   cACGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTActtaccttac                                >  1:692083/1‑68 (MQ=255)

                                   cACGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTActtaccttac                                >  1:737720/1‑68 (MQ=255)

                                   cACGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTActtaccttac                                >  1:1853974/1‑68 (MQ=255)

                                   cACGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTActtaccttac                                >  1:1276140/1‑68 (MQ=255)

                                     cGCCAGATCTCAGTATGAACGGAAAAATGATTTATGCAGCAATTACTTACACCCTACTTACCTTACttt                             <  1:1600927/69‑1 (MQ=255)

                                     cGCCAGATCTCAGTATGAACGGAAAAATGATTTATGCAGCAATTACTTACACCCTACTTACCTTACttt                             <  1:1818093/69‑1 (MQ=255)

                                     cGCCAGATCTCAGTATGAACGGAAAAATGATTTATGCAGCAATTACTTACACCCTACTTACCTTACttt                             <  1:1499318/69‑1 (MQ=255)

                                     cGCCAGATCTCAGTATGAACGGAAAAATGATTTATGCAGCAATTACTTACACCCTACTTACCTTACttt                             <  1:2579151/69‑1 (MQ=255)

                                     cGCCAGATCTCAGCATGAACGGAAAAATGATTTATGCAGCAATTACTTACACCCTACTTACCTTACttt                             <  1:3163103/69‑1 (MQ=255)

                                      gCCAGATCTCAGTATGAACGGAAAAATGATTTATGCAGCAATTACTTACACCCTACTTACCTTACttt                             <  1:1223696/68‑1 (MQ=255)

                                      gCCAGATCTCAGTATGAACGGAAAAATGATTTATGCAGCAATTACTTACACCCTACTTACCTTACttt                             <  1:1817400/68‑1 (MQ=255)

                                          gATCTCAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATAcc                        <  1:1886783/69‑1 (MQ=255)

                                          gATCTCAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATAcc                        <  1:1500972/69‑1 (MQ=255)

                                          gATCTCAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATAcc                        <  1:1264697/69‑1 (MQ=255)

                                             ctcAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTActtacct                                   >  1:646086/1‑55 (MQ=255)

                                             ctcAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTActtacct                                   >  1:1752197/1‑55 (MQ=255)

                                             ctcAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTActtacct                                   >  1:1748320/1‑55 (MQ=255)

                                             ctcAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTActtacct                                   >  1:3346835/1‑55 (MQ=255)

                                             ctcAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTActtacct                                   >  1:1045373/1‑55 (MQ=255)

                                             ctcAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTActtacct                                   >  1:655843/1‑55 (MQ=255)

                                             ctcAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTActtacct                                   >  1:123091/1‑55 (MQ=255)

                                             ctcAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTActtacct                                   >  1:1144602/1‑55 (MQ=255)

                                             ctcAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTActtacct                                   >  1:2086836/1‑55 (MQ=255)

                                             ctcAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTActtacct                                   >  1:245586/1‑55 (MQ=255)

                                             ctcAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTActtacct                                   >  1:1813401/1‑55 (MQ=255)

                                                  tATGAACTGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACCGTCGTCaa                <  1:2533934/69‑1 (MQ=255)

                                                  tATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACCgtcgtc                  <  1:2803944/67‑1 (MQ=255)

                                                  tATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACCgtcgtc                  <  1:1127346/67‑1 (MQ=255)

                                                  tATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACCgtcgtc                  <  1:2043669/67‑1 (MQ=255)

                                                  tATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACCGTCGTCaa                <  1:3397028/69‑1 (MQ=255)

                                                  tATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACCGTCGTCaa                >  1:2215162/1‑69 (MQ=255)

                                                  tATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACCGTCGTCaa                <  1:736224/69‑1 (MQ=255)

                                                  tATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACCGTCGTCaa                <  1:1249005/69‑1 (MQ=255)

                                                  tATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACCGTCGTCaa                <  1:574500/69‑1 (MQ=255)

                                                  tATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACCGTCGTCaa                <  1:2518170/69‑1 (MQ=255)

                                                  tATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACCGTCGTCaa                <  1:3403318/69‑1 (MQ=255)

                                                  tATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACCGTCGTCaa                <  1:1766498/69‑1 (MQ=255)

                                                  tATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACCGTCGTCaa                <  1:335481/69‑1 (MQ=255)

                                                  tATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACCGTCGTCaa                >  1:3320386/1‑69 (MQ=255)

                                                  tATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACCGTCGTCaa                >  1:3172243/1‑69 (MQ=255)

                                                   aTGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACCGTCGTCaa                <  1:1719040/68‑1 (MQ=255)

                                                   aTGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACCGTCGTCaa                <  1:2041920/68‑1 (MQ=255)

                                                    tGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACCGTCGTCAata              >  1:2738735/1‑69 (MQ=255)

                                                    tGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACCGTCGTCAata              >  1:1705361/1‑69 (MQ=255)

                                                    tGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACCGTCGTCAata              >  1:2420141/1‑69 (MQ=255)

                                                    tGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACCGTCGTCAata              >  1:2488177/1‑69 (MQ=255)

                                                    tGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTAACCTTACTTTATACCGTCGTCAata              >  1:2768219/1‑69 (MQ=255)

                                                      aaCGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACCGTCGTGAaaata            >  1:1392156/1‑68 (MQ=255)

                                                      aaCGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACCGTCGTCAatat             >  1:121026/1‑68 (MQ=255)

                                                      aaCGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACCGTCGTCAatat             >  1:3234015/1‑68 (MQ=255)

                                                      aaCGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACCGTCGTCAATATc            >  1:2657752/1‑69 (MQ=255)

                                                      aaCGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACCGTCGTCAATATc            >  1:2444491/1‑69 (MQ=255)

                                                      aaCGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACCGTCGTCAATATc            >  1:1553512/1‑69 (MQ=255)

                                                      aaCGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACCGTCGTCAATATc            >  1:2638863/1‑69 (MQ=255)

                                                             aaaTGATTTATGCAGCAATTACTTACACCCTACTTACCTTACttt                             <  1:1499320/45‑1 (MQ=255)

                                                                tGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACCGTCGTCAATATCCCTTACTgcg  <  1:2798025/69‑1 (MQ=255)

CTGCCATTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACCGTCGTCAATATCCCTTACTGCG  >  minE/2505806‑2505938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`