BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,506,486	C→T	32.4%	P314P (CCC→CCT)	yicJ →	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,506,486	0	C	T	32.4%	109.9 / 62.9	123	P314P (CCC→CCT)	yicJ	predicted transporter
Reads supporting (aligned to +/- strand): ref base C (55/28); new base T (18/22); total (73/50)
Fisher's exact test for biased strand distribution p-value = 3.14e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 123 total aligned reads displayed.

AAGTCACTATCTTCTGGTGGACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACT  >  minE/2506419‑2506545

aaGTCACTATCTTCTGGTGGACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCa                                                            <  1:1045595/69‑1 (MQ=255)

    cACTATCTTCTGGTGGACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCa                                                        >  1:1696623/1‑69 (MQ=255)

    cACTATCTTCTGGTGGACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCa                                                        >  1:1449996/1‑69 (MQ=255)

    cACTATCTTCTGGTGGACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCa                                                        >  1:795778/1‑69 (MQ=255)

    cACTATCTTCTGGTGGACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCa                                                        >  1:122055/1‑69 (MQ=255)

    cACTATCTTCTGGTGGACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCa                                                        >  1:101482/1‑69 (MQ=255)

     aCTATCTTCTGGTGGACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATTTTCTTTGTTCCTATGCAg                                                       <  1:1055401/69‑1 (MQ=255)

     aCTATCTTCTGGTGGACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCTATGCAg                                                       <  1:1714632/69‑1 (MQ=255)

     aCTATCTTCTGGTGGACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCTATGCAg                                                       <  1:1904730/69‑1 (MQ=255)

     aCTATCTTCTGGTGGACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCTATGCAg                                                       <  1:1844779/69‑1 (MQ=255)

     aCTATCTTCTGGTGGACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCTATGCAg                                                       <  1:1996140/69‑1 (MQ=255)

     aCTATCTTCTGGTGGACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCTATGCAg                                                       <  1:2094275/69‑1 (MQ=255)

     aCTATCTTCTGGTGGACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCTATGCAg                                                       <  1:2616934/69‑1 (MQ=255)

     aCTATCTTCTGGTGGACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCTATGCAg                                                       <  1:2933607/69‑1 (MQ=255)

     aCTATCTTCTGGTGGACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCTATGCAg                                                       <  1:2985850/69‑1 (MQ=255)

     aCTATCTTCTGGTGGACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCTATGCAg                                                       <  1:3151979/69‑1 (MQ=255)

     aCTATCTTCTGGTGGACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCTATGCAg                                                       <  1:3225714/69‑1 (MQ=255)

     aCTATCTTCTGGTGGACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCTATGCAg                                                       <  1:427098/69‑1 (MQ=255)

     aCTATCTTCTGGTGGACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCTATGCAg                                                       <  1:540420/69‑1 (MQ=255)

     aCTATCTTCTGGTGGACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCTATGCAg                                                       <  1:1368451/69‑1 (MQ=255)

     aCTATCTTCTGGTGGACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCTATGCAg                                                       <  1:541912/69‑1 (MQ=255)

     aCTATCTTCTGGTGGACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCTATGCAg                                                       <  1:134021/69‑1 (MQ=255)

     aCTATCTTCTGGTGGACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCTATGCAg                                                       <  1:605683/69‑1 (MQ=255)

     aCTATCTTCTGGTGGACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCTATGCAg                                                       <  1:793474/69‑1 (MQ=255)

     aCTATCTTCTGGTGGACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCTATGCAg                                                       <  1:1265904/69‑1 (MQ=255)

     aCTATCTTCTGGTGGACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCTATGCAg                                                       <  1:912987/69‑1 (MQ=255)

           ttctGGTGGACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAg                                                  <  1:677996/68‑1 (MQ=255)

           ttctGGTGGACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAg                                                  <  1:951915/68‑1 (MQ=255)

           ttctGGTGGACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAg                                                  <  1:1416817/68‑1 (MQ=255)

                  ggACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCAc                                            <  1:2139347/67‑1 (MQ=255)

                              tgctgGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCTATGCAg                                                       <  1:2801750/44‑1 (MQ=255)

                              tgctgGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCa                              <  1:126783/69‑1 (MQ=255)

                                        gATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATc                            >  1:3152665/1‑61 (MQ=255)

                                        gATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGt                       >  1:1478886/1‑66 (MQ=255)

                                        gATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGt                       >  1:1308128/1‑66 (MQ=255)

                                        gATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGt                       >  1:2215601/1‑66 (MQ=255)

                                        gATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGt                       >  1:3298585/1‑66 (MQ=255)

                                        gATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGt                       >  1:2300936/1‑66 (MQ=255)

                                        gATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGAt                    >  1:931343/1‑69 (MQ=255)

                                        gATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGAt                    >  1:1873424/1‑69 (MQ=255)

                                         aTTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCa                                             >  1:2664139/1‑43 (MQ=255)

                                         aTTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCt                           >  1:2364201/1‑61 (MQ=255)

                                         aTTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGt                       <  1:660119/65‑1 (MQ=255)

                                         aTTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGt                       <  1:1457713/65‑1 (MQ=255)

                                         aTTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGt                       <  1:3226404/65‑1 (MQ=255)

                                         aTTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGt                       <  1:2641402/65‑1 (MQ=255)

                                         aTTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGt                       <  1:2114378/65‑1 (MQ=255)

                                         aTTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGt                       <  1:1683142/65‑1 (MQ=255)

                                         aTTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGt                       >  1:1983089/1‑65 (MQ=255)

                                         aTTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTg                      >  1:2833950/1‑66 (MQ=255)

                                         aTTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTg                      >  1:1005170/1‑66 (MQ=255)

                                         aTTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTg                      >  1:826377/1‑66 (MQ=255)

                                         aTTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTg                      >  1:1507145/1‑66 (MQ=255)

                                         aTTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTg                      >  1:3395978/1‑66 (MQ=255)

                                         aTTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTg                      >  1:1447234/1‑66 (MQ=255)

                                         aTTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTg                      >  1:1290204/1‑66 (MQ=255)

                                         aTTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTg                      >  1:794576/1‑66 (MQ=255)

                                         aTTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTg                      >  1:223981/1‑66 (MQ=255)

                                         aTTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTg                      >  1:2770039/1‑66 (MQ=255)

                                         aTTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTg                      >  1:2334615/1‑66 (MQ=255)

                                         aTTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTg                      >  1:2451495/1‑66 (MQ=255)

                                         aTTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTg                      >  1:2036697/1‑66 (MQ=255)

                                         aTTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGAtt                   >  1:2703626/1‑69 (MQ=255)

                                         aTTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGAtt                   >  1:1332898/1‑69 (MQ=255)

                                         aTTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGAtt                   >  1:538569/1‑69 (MQ=255)

                                         aTTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGAtt                   >  1:2361471/1‑69 (MQ=255)

                                         aTTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGAtt                   >  1:2420805/1‑69 (MQ=255)

                                         aTTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGAtt                   >  1:2965001/1‑69 (MQ=255)

                                         aTTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGAtt                   >  1:1040344/1‑69 (MQ=255)

                                          ttAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTg                  >  1:2892370/1‑69 (MQ=255)

                                                tCGCGTTGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGgtgtgt            <  1:2826417/69‑1 (MQ=255)

                                                tCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGgtgtgt            <  1:2940272/69‑1 (MQ=255)

                                                tCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGgtgtgt            <  1:606249/69‑1 (MQ=255)

                                                tCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGgtgtgt            <  1:2485498/69‑1 (MQ=255)

                                                tCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGgtgtgt            <  1:676030/69‑1 (MQ=255)

                                                tCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGgtgtgt            <  1:871306/69‑1 (MQ=255)

                                                tCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGgtgtgt            <  1:3222234/69‑1 (MQ=255)

                                                tCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGgtgtgt            <  1:48905/69‑1 (MQ=255)

                                                          cTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACt  >  1:2235542/1‑69 (MQ=255)

                                                          cTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACt  >  1:2810788/1‑69 (MQ=255)

                                                          cTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACt  >  1:3427022/1‑69 (MQ=255)

                                                          cTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACt  >  1:2107564/1‑69 (MQ=255)

                                                          cTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACt  >  1:2374398/1‑69 (MQ=255)

                                                          cTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACt  >  1:2901010/1‑69 (MQ=255)

                                                          cTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACt  >  1:3168632/1‑69 (MQ=255)

                                                          cTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACt  >  1:2495228/1‑69 (MQ=255)

                                                          cTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACt  >  1:2015750/1‑69 (MQ=255)

                                                              gTTCCTATGCAGGCCAGCATCACCATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCAt       >  1:1670108/1‑60 (MQ=255)

                                                              gTTCCTATGCAGGCCAGCATCACCATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATc      >  1:1739574/1‑61 (MQ=255)

                                                              gTTCCTATGCAGGCCAGCATCACCATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATc      >  1:1808163/1‑61 (MQ=255)

                                                              gTTCCTATGCAGGCCAGCATCACCATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATc      >  1:135458/1‑61 (MQ=255)

                                                              gTTCCTATGCAGGCCAGCATCACCATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATc      >  1:3404522/1‑61 (MQ=255)

                                                              gTTCCTATGCAGGCCAGCATCACCATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATc      >  1:3389912/1‑61 (MQ=255)

                                                              gTTCCTATGCAGGCCAGCATCACCATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATc      >  1:3175895/1‑61 (MQ=255)

                                                              gTTCCTATGCAGGCCAGCATCACCATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATc      >  1:154684/1‑61 (MQ=255)

                                                              gTTCCTATGCAGGCCAGCATCACCATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATc      >  1:1515849/1‑61 (MQ=255)

                                                              gTTCCTATGCAGGCCAGCATCACCATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATc      >  1:2882230/1‑61 (MQ=255)

                                                              gTTCCTATGCAGGCCAGCATCACCATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATc      >  1:2849750/1‑61 (MQ=255)

                                                              gTTCCTATGCAGGCCAGCATCACCATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATc      >  1:2638337/1‑61 (MQ=255)

                                                              gTTCCTATGCAGGCCAGCATCACCATGTTTGTCTTCATCTTAGTGATTGGTGTGTTGCATc      >  1:1978881/1‑61 (MQ=255)

AAGTCACTATCTTCTGGTGGACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACT  >  minE/2506419‑2506545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`